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The map label for this gene is 86609266

Identifier: 86609266

GI number: 86609266

Start: 1892268

End: 1893431

Strand: Reverse

Name: 86609266

Synonym: CYB_1808

Alternate gene names: NA

Gene position: 1893431-1892268 (Counterclockwise)

Preceding gene: 86609267

Following gene: 86609265

Centisome position: 62.15

GC content: 59.97

Gene sequence:

>1164_bases
ATGAAAGACAAGCTGATATTGATGATCCCCGGCCCCACTCCCGTTCCCGAGAGTGCGCTGCTGGCGATGGCACGGGGGCC
CATCGAGCACCGCAGCAAAGAGTTCACCGCCATTTTGGAAGAGGTCACCGATGGGTTGCGCTGGCTGCACCAAACCACCT
CCGATGTGTTTGTCTTTTCCGCCAGTGGCACCGGCGCTATGGAAGCCGGGATCCTCAACACCCTCAGCCCTGGGGATCGG
GTTTTGGTGGGGGTGAACGGCAAGTTTGGGGAGCGCTGGGCGGAAATGTCCGAGCAGTTTGGCCTTCAGTGTGAGCGGGT
GGAAACCCCCTGGGGGATCCCTTACCCCGTCGAGATCTTCCAAGAAAAGCTGGCTGCCGACCGGGAAAAAACCATCAAAG
CCGTCATCCTCACCCACTCGGAGACTTCCAGCGGCGTAGCCAACGATGTCCAAGCCATTGCTGCTGCTGCCCGCGAGCAC
GGGGAAGCCCTCGTCCTGGTGGATGGGGTCACCAGCGTGGGGGCAATGCCGGTGTTAATGGACGAATGGGGCTTGGATGT
GGTGGCCTCGGGATCCCAGAAGGGCTATATGATCCCACCAGGGCTGGGATTTGTGGCCGTCAGCCCGCGGGCCATGGCCG
CTACGGAGCGCTCTAGGTTTCCCAAGTACTACTGGAGCTTTAAGCTGGCGCAAAAGGCCCTGCGCCAAGGCACCACTCCC
TTTACACCGGCGGTGAACCTGTTTTATGCCCTGCGAACGACGTTACAGATGATGCGGGCAGAGGGTTTGGAGGCCATTTA
CCGGCGTCATGCCAGGTTATCCCAAGCCACCCGTGCTGGCGTCAAGGCTCTGGGGCTAAAGCTCTTGGTTGATCCAGAGT
CAGCGGCCAGTCCTTCGGTAACAGCTGTACTCGCTCCCGAAGGGATCAATGCCGATACCCTTCGCAGCACCTTGAAGAAA
CACTTCGACATCGCCCTTGCCGCTGGACAAGACCACCTCAAGGGCAAAATCTTCCGCATTGGCCATTTGGGCTTTGTGAG
TGACCGCGATGTGCTGATGACCTTGGCTGCTCTGGAGTCAGCTTTACACACCGTGGGCTACAGCGACTTCACCCCAGGGG
CGGGAACTCGCGCCGCCGAGGAAGTACTGGCCCACGGCTTCTGA

Upstream 100 bases:

>100_bases
GTAGCCAAGTGGTAAGGCAGAGGTCTGCAAAACCTCTATGCGCCAGTTCGAATCTGGCCGCCACCTTTTAAACCTCACTT
GCATCCCAAGCAGGGCAAGC

Downstream 100 bases:

>100_bases
GCCCCCAGCTCAGTAGTCTTTGACCCATAGCACTGACCCTTGCCCCTGGCTACAATGGGGGGACATGCTTAATAAAACTT
CAGAACCCAAGGGTGGCTAT

Product: soluble hydrogenase, tritium exchange subunit

Products: NA

Alternate protein names: Tritium exchange subunit [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKDKLILMIPGPTPVPESALLAMARGPIEHRSKEFTAILEEVTDGLRWLHQTTSDVFVFSASGTGAMEAGILNTLSPGDR
VLVGVNGKFGERWAEMSEQFGLQCERVETPWGIPYPVEIFQEKLAADREKTIKAVILTHSETSSGVANDVQAIAAAAREH
GEALVLVDGVTSVGAMPVLMDEWGLDVVASGSQKGYMIPPGLGFVAVSPRAMAATERSRFPKYYWSFKLAQKALRQGTTP
FTPAVNLFYALRTTLQMMRAEGLEAIYRRHARLSQATRAGVKALGLKLLVDPESAASPSVTAVLAPEGINADTLRSTLKK
HFDIALAAGQDHLKGKIFRIGHLGFVSDRDVLMTLAALESALHTVGYSDFTPGAGTRAAEEVLAHGF

Sequences:

>Translated_387_residues
MKDKLILMIPGPTPVPESALLAMARGPIEHRSKEFTAILEEVTDGLRWLHQTTSDVFVFSASGTGAMEAGILNTLSPGDR
VLVGVNGKFGERWAEMSEQFGLQCERVETPWGIPYPVEIFQEKLAADREKTIKAVILTHSETSSGVANDVQAIAAAAREH
GEALVLVDGVTSVGAMPVLMDEWGLDVVASGSQKGYMIPPGLGFVAVSPRAMAATERSRFPKYYWSFKLAQKALRQGTTP
FTPAVNLFYALRTTLQMMRAEGLEAIYRRHARLSQATRAGVKALGLKLLVDPESAASPSVTAVLAPEGINADTLRSTLKK
HFDIALAAGQDHLKGKIFRIGHLGFVSDRDVLMTLAALESALHTVGYSDFTPGAGTRAAEEVLAHGF
>Mature_387_residues
MKDKLILMIPGPTPVPESALLAMARGPIEHRSKEFTAILEEVTDGLRWLHQTTSDVFVFSASGTGAMEAGILNTLSPGDR
VLVGVNGKFGERWAEMSEQFGLQCERVETPWGIPYPVEIFQEKLAADREKTIKAVILTHSETSSGVANDVQAIAAAAREH
GEALVLVDGVTSVGAMPVLMDEWGLDVVASGSQKGYMIPPGLGFVAVSPRAMAATERSRFPKYYWSFKLAQKALRQGTTP
FTPAVNLFYALRTTLQMMRAEGLEAIYRRHARLSQATRAGVKALGLKLLVDPESAASPSVTAVLAPEGINADTLRSTLKK
HFDIALAAGQDHLKGKIFRIGHLGFVSDRDVLMTLAALESALHTVGYSDFTPGAGTRAAEEVLAHGF

Specific function: Soluble hydrogenase catalyzes both production and consumption of hydrogen from suitable artificial electron donors or acceptors. This subunit catalyzes the tritium-exchange activity [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=371, Percent_Identity=31.5363881401617, Blast_Score=169, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17536281, Length=362, Percent_Identity=31.4917127071823, Blast_Score=157, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321079, Length=356, Percent_Identity=26.685393258427, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17530823, Length=367, Percent_Identity=29.4277929155313, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 41683; Mature: 41683

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKLILMIPGPTPVPESALLAMARGPIEHRSKEFTAILEEVTDGLRWLHQTTSDVFVFS
CCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ASGTGAMEAGILNTLSPGDRVLVGVNGKFGERWAEMSEQFGLQCERVETPWGIPYPVEIF
CCCCCCHHHCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHH
QEKLAADREKTIKAVILTHSETSSGVANDVQAIAAAAREHGEALVLVDGVTSVGAMPVLM
HHHHHCCHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCHHH
DEWGLDVVASGSQKGYMIPPGLGFVAVSPRAMAATERSRFPKYYWSFKLAQKALRQGTTP
HCCCCCEEECCCCCCEECCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
FTPAVNLFYALRTTLQMMRAEGLEAIYRRHARLSQATRAGVKALGLKLLVDPESAASPSV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCE
TAVLAPEGINADTLRSTLKKHFDIALAAGQDHLKGKIFRIGHLGFVSDRDVLMTLAALES
EEEECCCCCCHHHHHHHHHHHHCEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
ALHTVGYSDFTPGAGTRAAEEVLAHGF
HHHHCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKDKLILMIPGPTPVPESALLAMARGPIEHRSKEFTAILEEVTDGLRWLHQTTSDVFVFS
CCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ASGTGAMEAGILNTLSPGDRVLVGVNGKFGERWAEMSEQFGLQCERVETPWGIPYPVEIF
CCCCCCHHHCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHH
QEKLAADREKTIKAVILTHSETSSGVANDVQAIAAAAREHGEALVLVDGVTSVGAMPVLM
HHHHHCCHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHCCCCHHH
DEWGLDVVASGSQKGYMIPPGLGFVAVSPRAMAATERSRFPKYYWSFKLAQKALRQGTTP
HCCCCCEEECCCCCCEECCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
FTPAVNLFYALRTTLQMMRAEGLEAIYRRHARLSQATRAGVKALGLKLLVDPESAASPSV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCE
TAVLAPEGINADTLRSTLKKHFDIALAAGQDHLKGKIFRIGHLGFVSDRDVLMTLAALES
EEEECCCCCCHHHHHHHHHHHHCEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
ALHTVGYSDFTPGAGTRAAEEVLAHGF
HHHHCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2513553 [H]