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The map label for this gene is wcaJ [H]

Identifier: 86609241

GI number: 86609241

Start: 1868929

End: 1870488

Strand: Direct

Name: wcaJ [H]

Synonym: CYB_1783

Alternate gene names: 86609241

Gene position: 1868929-1870488 (Clockwise)

Preceding gene: 86609240

Following gene: 86609242

Centisome position: 61.34

GC content: 59.1

Gene sequence:

>1560_bases
TTGGCCGAACGCGGCTATAAAACTCGTCTGGATCTGCATCGTTTGTGGGTGCCCTTGGCCTGTTTTGGCGATGGCCTTTG
TATTTGGTTGGCCTTTGGGGCGGGCTACTGGCTGCGCAGCTTGCGTCCTCATCAGCTCTTGCCTTGGGATGTCTACGCGG
CCTTGGGATTGTTGACCGCCCTCAGTTATTGGGGCATTCAGGCGGGCTTGGGCGGATACGGTGAGTCGAAGCGCTTGTCT
CGCTTTGAAGAATGGGCCAGTTTGCTGGTGGGGTTGGGATTGACGTTTGGTTTGGTGCTGGCAGGGGCCTTTTTCGTTCG
CTCGGTCTCTTTGTCGCGGCTGGTGGTGGGCTACGCCGGCTTGTTGGCTCTGCTGAGTTTGACTTTGGTACGGGTGGGGT
TGCGGCTGGGCATGGGCTGGTTGTGCTCTAGGGGCTACGGTCGGCGGCAGGTCATCTGGGTGGGATCCTGGTCGGTGTTG
GCGGAAGTGTTGGAGCGATTGCAGCGATCCCCGCAGTTGGGCTTCCAGGTAGTCGCCTGGGCAGGCGTAGAGCAAGGCTC
TTTTGAGGAAGATCCCCCATCTCAAAATGGGGATCCCTGTCAAGATTCATATCCAGTACTAAACACCGCCCGCCCTACCT
CTGGCGGGGTTGAGTCTGCCGACAGGACTCTGTCCGGCTCATCCCGAGGAACCCTCTCCTGCTTGTGCAGTCAGCTTGAG
CCTTCCTGGTCGGCTCTTGAGCGTCTGGATCTGGTCCATTTGATTGCCTATCTGCAAGCCCACCCAGAAGTGCAGGAGGT
GTGGTTTGGGCAACCGGAGCTGGCAACTGCTCCTCTGTTGCTCAGTTTGCAAACATTGTCGGTGCAGGTGCGCTTGGTGC
CGGATATCTTAAGTTTTTTAACGGTGAACTTCAGCCTGCAGTCTCTCGACGGGATCCCGCTGCTAACTCTGCGTCAGCCC
CCTTTGCGCTACGCCCACAACCGGTTCCTCAAGCGAGCTGTAGACATCCTAGGAGCTTCCCTGGGCCTGATCTTGCTTAG
CCCTCTGTTTTTGATTATTGCCCTGCTGGTAGCCTGCAGCAGTCCTGGCCCCATTCTGTATTGGCAAGAGCGGGTGAGCT
TGGACGGGCGTCGCTTTTGGATTGCCAAGTTCCGCACCATGCGAGTGGATGCTGAGGCAGCCGGCCAGCCGGGCTGGACG
CGCCCCGGGGATCCGCGCCGAACCAAGATAGGAGCCTTTTTGCGCCGCACCAACCTGGACGAGCTGCCCCAACTGTGGAA
CGTCCTGAAAGGGGAAATGAGCCTAGTGGGCCCCCGCCCAGAGCGGCCCTACTTTGTCGAGCAGTTTAGCCAGGAGATCC
CCAAGTATCCGGATCGGCATTTGGTAAAAAGTGGCCTCACCGGCTGGGCCCAGATCCACGGCCTGCGGGGAGATACCAGC
ATTGCCCGCCGCACCCAGTATGATCTCTACTATGTGCAAAACTGGTCGCTGTGGCTGGATCTGCGCATCCTGGCCATCAC
CTTCTGGCAAGGGATCCGCGGACGGCTGGCCGGATACTAG

Upstream 100 bases:

>100_bases
CCATTCAAGTTTTGGTCAACACTGATCTTGTCCAATAGCCTCAGATGCACAATCTTTTCTGTAAGATTGTCAACAGTCTT
GATGCCGAAGGAGATAACCC

Downstream 100 bases:

>100_bases
GGGTTAGCGTATGCCATTCTCCCCCCAAGAGCTGCTCTACGAGGGCAAGGCCAAGCGCATCTACCGCACTGCCGATCCCA
GGGTTTATCTTTGCCAATAC

Product: sugar transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAERGYKTRLDLHRLWVPLACFGDGLCIWLAFGAGYWLRSLRPHQLLPWDVYAALGLLTALSYWGIQAGLGGYGESKRLS
RFEEWASLLVGLGLTFGLVLAGAFFVRSVSLSRLVVGYAGLLALLSLTLVRVGLRLGMGWLCSRGYGRRQVIWVGSWSVL
AEVLERLQRSPQLGFQVVAWAGVEQGSFEEDPPSQNGDPCQDSYPVLNTARPTSGGVESADRTLSGSSRGTLSCLCSQLE
PSWSALERLDLVHLIAYLQAHPEVQEVWFGQPELATAPLLLSLQTLSVQVRLVPDILSFLTVNFSLQSLDGIPLLTLRQP
PLRYAHNRFLKRAVDILGASLGLILLSPLFLIIALLVACSSPGPILYWQERVSLDGRRFWIAKFRTMRVDAEAAGQPGWT
RPGDPRRTKIGAFLRRTNLDELPQLWNVLKGEMSLVGPRPERPYFVEQFSQEIPKYPDRHLVKSGLTGWAQIHGLRGDTS
IARRTQYDLYYVQNWSLWLDLRILAITFWQGIRGRLAGY

Sequences:

>Translated_519_residues
MAERGYKTRLDLHRLWVPLACFGDGLCIWLAFGAGYWLRSLRPHQLLPWDVYAALGLLTALSYWGIQAGLGGYGESKRLS
RFEEWASLLVGLGLTFGLVLAGAFFVRSVSLSRLVVGYAGLLALLSLTLVRVGLRLGMGWLCSRGYGRRQVIWVGSWSVL
AEVLERLQRSPQLGFQVVAWAGVEQGSFEEDPPSQNGDPCQDSYPVLNTARPTSGGVESADRTLSGSSRGTLSCLCSQLE
PSWSALERLDLVHLIAYLQAHPEVQEVWFGQPELATAPLLLSLQTLSVQVRLVPDILSFLTVNFSLQSLDGIPLLTLRQP
PLRYAHNRFLKRAVDILGASLGLILLSPLFLIIALLVACSSPGPILYWQERVSLDGRRFWIAKFRTMRVDAEAAGQPGWT
RPGDPRRTKIGAFLRRTNLDELPQLWNVLKGEMSLVGPRPERPYFVEQFSQEIPKYPDRHLVKSGLTGWAQIHGLRGDTS
IARRTQYDLYYVQNWSLWLDLRILAITFWQGIRGRLAGY
>Mature_518_residues
AERGYKTRLDLHRLWVPLACFGDGLCIWLAFGAGYWLRSLRPHQLLPWDVYAALGLLTALSYWGIQAGLGGYGESKRLSR
FEEWASLLVGLGLTFGLVLAGAFFVRSVSLSRLVVGYAGLLALLSLTLVRVGLRLGMGWLCSRGYGRRQVIWVGSWSVLA
EVLERLQRSPQLGFQVVAWAGVEQGSFEEDPPSQNGDPCQDSYPVLNTARPTSGGVESADRTLSGSSRGTLSCLCSQLEP
SWSALERLDLVHLIAYLQAHPEVQEVWFGQPELATAPLLLSLQTLSVQVRLVPDILSFLTVNFSLQSLDGIPLLTLRQPP
LRYAHNRFLKRAVDILGASLGLILLSPLFLIIALLVACSSPGPILYWQERVSLDGRRFWIAKFRTMRVDAEAAGQPGWTR
PGDPRRTKIGAFLRRTNLDELPQLWNVLKGEMSLVGPRPERPYFVEQFSQEIPKYPDRHLVKSGLTGWAQIHGLRGDTSI
ARRTQYDLYYVQNWSLWLDLRILAITFWQGIRGRLAGY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=400, Percent_Identity=34.25, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 58070; Mature: 57939

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERGYKTRLDLHRLWVPLACFGDGLCIWLAFGAGYWLRSLRPHQLLPWDVYAALGLLTA
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LSYWGIQAGLGGYGESKRLSRFEEWASLLVGLGLTFGLVLAGAFFVRSVSLSRLVVGYAG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLALLSLTLVRVGLRLGMGWLCSRGYGRRQVIWVGSWSVLAEVLERLQRSPQLGFQVVAW
HHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEE
AGVEQGSFEEDPPSQNGDPCQDSYPVLNTARPTSGGVESADRTLSGSSRGTLSCLCSQLE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCC
PSWSALERLDLVHLIAYLQAHPEVQEVWFGQPELATAPLLLSLQTLSVQVRLVPDILSFL
CCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHEEEEEHEEEEEEHHHHHHHH
TVNFSLQSLDGIPLLTLRQPPLRYAHNRFLKRAVDILGASLGLILLSPLFLIIALLVACS
HHCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPGPILYWQERVSLDGRRFWIAKFRTMRVDAEAAGQPGWTRPGDPRRTKIGAFLRRTNLD
CCCCEEEEHHHCCCCCCEEEEEEHHEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHCCHH
ELPQLWNVLKGEMSLVGPRPERPYFVEQFSQEIPKYPDRHLVKSGLTGWAQIHGLRGDTS
HHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCHH
IARRTQYDLYYVQNWSLWLDLRILAITFWQGIRGRLAGY
HHHHHEEEEEEEECCEEEHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AERGYKTRLDLHRLWVPLACFGDGLCIWLAFGAGYWLRSLRPHQLLPWDVYAALGLLTA
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LSYWGIQAGLGGYGESKRLSRFEEWASLLVGLGLTFGLVLAGAFFVRSVSLSRLVVGYAG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLALLSLTLVRVGLRLGMGWLCSRGYGRRQVIWVGSWSVLAEVLERLQRSPQLGFQVVAW
HHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEEE
AGVEQGSFEEDPPSQNGDPCQDSYPVLNTARPTSGGVESADRTLSGSSRGTLSCLCSQLE
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCC
PSWSALERLDLVHLIAYLQAHPEVQEVWFGQPELATAPLLLSLQTLSVQVRLVPDILSFL
CCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHEEEEEHEEEEEEHHHHHHHH
TVNFSLQSLDGIPLLTLRQPPLRYAHNRFLKRAVDILGASLGLILLSPLFLIIALLVACS
HHCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPGPILYWQERVSLDGRRFWIAKFRTMRVDAEAAGQPGWTRPGDPRRTKIGAFLRRTNLD
CCCCEEEEHHHCCCCCCEEEEEEHHEEEECHHHCCCCCCCCCCCCCHHHHHHHHHHCCHH
ELPQLWNVLKGEMSLVGPRPERPYFVEQFSQEIPKYPDRHLVKSGLTGWAQIHGLRGDTS
HHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCCCCCCHH
IARRTQYDLYYVQNWSLWLDLRILAITFWQGIRGRLAGY
HHHHHEEEEEEEECCEEEHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]