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The map label for this gene is prfA [H]

Identifier: 86609051

GI number: 86609051

Start: 1656499

End: 1657581

Strand: Direct

Name: prfA [H]

Synonym: CYB_1586

Alternate gene names: 86609051

Gene position: 1656499-1657581 (Clockwise)

Preceding gene: 86609050

Following gene: 86609052

Centisome position: 54.37

GC content: 60.3

Gene sequence:

>1083_bases
ATGGCTTCTGCTCAACTGATCGACCAGCTTAAGAATGTCGAAGCCACTTTTCAGGAGCTGACGGTGCGCTTGGCCGATCC
GGACGTGGCCACGGATCCAGCTGAGCTGCAGCGGATTGCCAAGGCCCGCTCCTCCTTGGAGGCCACCGTCCAGGCTTTTC
ACGACTGGCAAAAGCTGAATCGCGATCTGCAACAGACGGAAGAACTTTTGCGGGAATCGGCCTCCGACCCGGAGCTGGAG
GCGATGGCAAGAGCGGAACTTGAGCAACTGCGGGCCCGCCAAGAACAACTGGAGGAAAAGCTCACCCTGCTGCTGCTGCC
CCGGGATCCCAACGACGACAAGAACATCATGTTGGAGATTCGCGCCGGCACCGGTGGGGAGGAGGCGGCGCTGTGGGCGG
CGGATCTGGCCCGCATGTACACCCGCTACGCCGAAAAGCTGGGCTGGTCGGTGCGTCTGGCGAGCCTCTCGGAAGGAGAG
CTGGGGGGCTACAAAGAGGCGATCCTGGAGATTCAAGGGGATCAGGTGTACAGCAAGCTCAAGTTTGAAGCTGGCGTGCA
TCGGGTGCAGCGGGTGCCCGTCACCGAAGCGCAAGGCCGTGTCCACACCTCGACGGCCACGGTGGCGGTGATGCCAGAGG
TGGACGAGGTGGAGGTGGTCATCGACCCGAAAGACATCGAAATCAAAACGGCACGCTCCGGGGGGGCAGGCGGCCAAAAC
GTGAACAAGGTGGAGACGGCGGTGGATCTTCTCCACAAGCCCACCGGGATCCGGGTGTTTTGTACCGAGGAGCGTTCCCA
ACTGCAGAACCGCCAGCGGGCCATGCAGATTTTGCGGGCCAAACTCTACGAGATGAAGCTGCAGGAGCAACAGGCCTCCA
TCAGCTCTGCTCGGCGCATGCAGGTGGGAACGGGATCCCGCTCGGAAAAAATTCGCACCTACAACTACAAGGACAACCGC
GTTACCGACCACCGCCTTAACCAGAATTTCCCTCTGCAGCCCATCTTGGATGGGGAGCTGGACGACTTGATCCAAGCTTG
TATTGGCCTTTACCAAAAAGAGCTGTTGGCGGAGCTATCCTAG

Upstream 100 bases:

>100_bases
GTTCATGCGCAAATACGGCATGAGCGGTGGCAAAAAGGCCAAGTAGAGGTTCCCCCATCTGGGTGTGGGCAACGCTTGGA
TCCCTGGGTATTGCGAATCT

Downstream 100 bases:

>100_bases
GCCAAGCTGAGTTACCGCCAGCGGATGGGATCCCTTCTCGAGGACCTTTCCTCTGCCGAGCTAGCGGTAGAATAAATACC
CACGTCGAGTGCCTAAACCC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MASAQLIDQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQAFHDWQKLNRDLQQTEELLRESASDPELE
AMARAELEQLRARQEQLEEKLTLLLLPRDPNDDKNIMLEIRAGTGGEEAALWAADLARMYTRYAEKLGWSVRLASLSEGE
LGGYKEAILEIQGDQVYSKLKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRMQVGTGSRSEKIRTYNYKDNR
VTDHRLNQNFPLQPILDGELDDLIQACIGLYQKELLAELS

Sequences:

>Translated_360_residues
MASAQLIDQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQAFHDWQKLNRDLQQTEELLRESASDPELE
AMARAELEQLRARQEQLEEKLTLLLLPRDPNDDKNIMLEIRAGTGGEEAALWAADLARMYTRYAEKLGWSVRLASLSEGE
LGGYKEAILEIQGDQVYSKLKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRMQVGTGSRSEKIRTYNYKDNR
VTDHRLNQNFPLQPILDGELDDLIQACIGLYQKELLAELS
>Mature_359_residues
ASAQLIDQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQAFHDWQKLNRDLQQTEELLRESASDPELEA
MARAELEQLRARQEQLEEKLTLLLLPRDPNDDKNIMLEIRAGTGGEEAALWAADLARMYTRYAEKLGWSVRLASLSEGEL
GGYKEAILEIQGDQVYSKLKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQNV
NKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRMQVGTGSRSEKIRTYNYKDNRV
TDHRLNQNFPLQPILDGELDDLIQACIGLYQKELLAELS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=44.7019867549669, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI34577120, Length=334, Percent_Identity=36.8263473053892, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=44.1025641025641, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=45.0980392156863, Blast_Score=303, Evalue=8e-84,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=38.0952380952381, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=306, Percent_Identity=37.9084967320261, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=40.6940063091483, Blast_Score=212, Evalue=7e-56,
Organism=Drosophila melanogaster, GI19921226, Length=325, Percent_Identity=38.4615384615385, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40504; Mature: 40373

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAQLIDQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQAFHDWQKLN
CCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDLQQTEELLRESASDPELEAMARAELEQLRARQEQLEEKLTLLLLPRDPNDDKNIMLEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCEEEEEE
RAGTGGEEAALWAADLARMYTRYAEKLGWSVRLASLSEGELGGYKEAILEIQGDQVYSKL
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHEECCHHHHHHH
KFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRM
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVGTGSRSEKIRTYNYKDNRVTDHRLNQNFPLQPILDGELDDLIQACIGLYQKELLAELS
ECCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASAQLIDQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQAFHDWQKLN
CHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDLQQTEELLRESASDPELEAMARAELEQLRARQEQLEEKLTLLLLPRDPNDDKNIMLEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCEEEEEE
RAGTGGEEAALWAADLARMYTRYAEKLGWSVRLASLSEGELGGYKEAILEIQGDQVYSKL
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHEECCHHHHHHH
KFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRM
HHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
QVGTGSRSEKIRTYNYKDNRVTDHRLNQNFPLQPILDGELDDLIQACIGLYQKELLAELS
ECCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA