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The map label for this gene is spoT [H]

Identifier: 86609008

GI number: 86609008

Start: 1617330

End: 1619612

Strand: Reverse

Name: spoT [H]

Synonym: CYB_1543

Alternate gene names: 86609008

Gene position: 1619612-1617330 (Counterclockwise)

Preceding gene: 86609009

Following gene: 86609007

Centisome position: 53.16

GC content: 56.02

Gene sequence:

>2283_bases
ATGGGTCAGACGGCTGTCATTGCCACCTCAGATGGAGGTCTCGACCTGAATAGCCTCGGTTTTGAGGTGCCAGGGTGGCT
GCGTGCTCTGCTTTCCGGTGCCGAGGATCCCGGCGATGGGCTGCGGCCCTACACGGCTGAAGAAGCCGATCTGCTGCGCC
GTGCCTTTGAGTTTTCTTACTGTTTGCATCAGGGCCAAAAACGCAAATCGGGGGATCCCTACATTGCCCATCCGGTAGCC
GTGGCTACTCTCCTGCGGGAGCTGGGGGGAGATGCGGTGACGGTGGCAGCGGGCCTTTTGCACGATGTTATTGAGGATAC
AGAAGTTACCCCAGAAGCCCTGGAAGCTGAATTCGGCTCTGAAGTTCGCCTACTGGTAGAAGGAGTTACCAAACTCTCTA
AGTTCAATTTCTCCAGCAAAACCGAGCAGCAAGCGGAAAATTTCCGCCGCATGTTTGTGGCAATGGCCAAAGATATTCGA
GTCATCGTGGTCAAGCTGGCGGATCGCTTGCACAACATGCGCACGCTGCAGTATCTCTCCCCCAGCAAGCAACGGCAGAT
TGCAGCCGAAACCATGGAAATTTTCGCCCCTTTGGCCAACCGGCTGGGGATCTGGCACTTCAAATGGGAACTGGAAGACT
TGGCTTTTAAGTATCTGGACTACGAGGCTTACCGGAAGATTCAGGAATTGGTCAATGCCAAGCGCGCCGAGCGAGAGGCC
GAGATCCAAGCTTTTATCGAAGAGCTGCGGCAGAACCTTCTTCAAGCGGGGCTGGAACATTTCGAGATCAGCGGGCGGCC
CAAACATTTGTACAGCATCTATCGCAAGATGCAGCAGCAGCACAAAGAATTTCACGAGATTTACGATCTCTCGGCAGTGC
GGGTAATCGTGCATACCAACAGCGAGTGCTATCGGGTGCTGGCAGTCGTTCACAACTGCTTTCGCCCAATCCCTGGGCGT
TTCAAAGACTACATTGGTCTGCCCAAGGCCAATCGTTACCAATCTTTGCATACGGCAGTGATCGGCCTGCAGGGACGACC
GGTGGAGGTGCAGATTCGTACCGAAGAGATGCACCGCGTGGCTGAGTACGGGATCGCCGCCCACTGGAAGTACAAGGAGG
CCGGCAGCACCACTGTCAAGCCCGACGAGGAGCGCTTCACCTGGCTGCGACAGCTGTTGGAGTGGCAAAACGACCTCAAA
GATGACAAGGAGTATCTGGACACCCTTCGCGAGAACCTGTTTGAGAGCGAGGTTTACGTCTTTACGCCCAAGGGGGACTT
GCTGGCTCTGCCCCAGGGATCCTGTCCGGTGGACTTCGCCTATCGCATCCACTCCGAAATTGGGGATCACTGTGCAGGAG
CGCGGGTAAACAACAAGATCGTGCCCCTGGATACCCGCCTGCGCAACGGCGACATCGTGCAGATCATTACCCACAAGAAT
GCCCATCCCAGCCTGGACTGGATCAATTTCGTTGCCACCAGCTCTGCCCGCAATCGCATTCGCCAATGGTTTAAGCGCTC
CAATCGCGAGCAAAACATCGCGCGGGGCCGGCATTTGCTGGAGCGGGAGCTGGGCAAAACGGGCTTTGATGCCCTGCTCA
AATCAGAGCGGATGCAGAAGGTAGCCGAAAAGCTCAACTACGCCAGTGTAGAGGATTTGTTAGCCGCTCTGGGTTATGGT
GAGACAACCCCCACGCTGGTGATCCATCGGCTGCAGGAGGTCAGCAGGCCGGCAGTATCCCCCCCTACAGAGGAGGAATC
GCTTGAGGGATCCAAGCTGTTGTTGCCGCGGGCTTCTGTGGCTTCCAGTTCCGTCAAATCCCAATCTCCCATTCTGGGGG
TGGAGGGTCTGAAACACCAGTTGGCCAAGTGTTGTAACCCGCTGCCGGGGGATCCGATTTTGGGCGTAGTCACCCGCTTT
AAGGGTATTAGTATCCACCATCAGGACTGTCCACGGGTGGCTCAGGTTTCGGGGGAACGCCTGGTGCCAGTACGCTGGAA
CGAAGAGCAACTGCGGCGGAGCACCCAGATCTATCCGGTAGAACTGCATCTGGAGGTTATCGACCGCGTTGGGGTGCTGC
GGGACATTCTTTCCCGTCTATCGGATCAAGGCATTAATGTTCGCAATGCTAAGGTACAAACTTTTGCCAACCGCACCGCC
CTGATCGATCTCTGTATCGATGTACGTGATAAGAAACAGCTCACCCAGGTTCAAAGTCAACTGCGCCAGCTTTCTGACGT
GATCGTGCTGCGCTCCCGCCATCACCAAAACGGATCCCGTTGA

Upstream 100 bases:

>100_bases
GCTTCTTTCTATTCCACCCGTTTTTTCCCTCTTGATCTGTCGGCGGTTAAGCTGGATGAACTATAGTCAGTAATCAAACA
TCCCTTTTCGCCGGGTTCGC

Downstream 100 bases:

>100_bases
CCTACGCCTGGCCCTTCGCGGGTACGGACTCTAGACCGCATCCCTATTGCGACTCGCTCCTGCAGCAGCCCAATTTTGGG
TCAGGCGCTGTCCCATCCTC

Product: RelA/SpoT family protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MGQTAVIATSDGGLDLNSLGFEVPGWLRALLSGAEDPGDGLRPYTAEEADLLRRAFEFSYCLHQGQKRKSGDPYIAHPVA
VATLLRELGGDAVTVAAGLLHDVIEDTEVTPEALEAEFGSEVRLLVEGVTKLSKFNFSSKTEQQAENFRRMFVAMAKDIR
VIVVKLADRLHNMRTLQYLSPSKQRQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRKIQELVNAKRAEREA
EIQAFIEELRQNLLQAGLEHFEISGRPKHLYSIYRKMQQQHKEFHEIYDLSAVRVIVHTNSECYRVLAVVHNCFRPIPGR
FKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRVAEYGIAAHWKYKEAGSTTVKPDEERFTWLRQLLEWQNDLK
DDKEYLDTLRENLFESEVYVFTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLDTRLRNGDIVQIITHKN
AHPSLDWINFVATSSARNRIRQWFKRSNREQNIARGRHLLERELGKTGFDALLKSERMQKVAEKLNYASVEDLLAALGYG
ETTPTLVIHRLQEVSRPAVSPPTEEESLEGSKLLLPRASVASSSVKSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRF
KGISIHHQDCPRVAQVSGERLVPVRWNEEQLRRSTQIYPVELHLEVIDRVGVLRDILSRLSDQGINVRNAKVQTFANRTA
LIDLCIDVRDKKQLTQVQSQLRQLSDVIVLRSRHHQNGSR

Sequences:

>Translated_760_residues
MGQTAVIATSDGGLDLNSLGFEVPGWLRALLSGAEDPGDGLRPYTAEEADLLRRAFEFSYCLHQGQKRKSGDPYIAHPVA
VATLLRELGGDAVTVAAGLLHDVIEDTEVTPEALEAEFGSEVRLLVEGVTKLSKFNFSSKTEQQAENFRRMFVAMAKDIR
VIVVKLADRLHNMRTLQYLSPSKQRQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRKIQELVNAKRAEREA
EIQAFIEELRQNLLQAGLEHFEISGRPKHLYSIYRKMQQQHKEFHEIYDLSAVRVIVHTNSECYRVLAVVHNCFRPIPGR
FKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRVAEYGIAAHWKYKEAGSTTVKPDEERFTWLRQLLEWQNDLK
DDKEYLDTLRENLFESEVYVFTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLDTRLRNGDIVQIITHKN
AHPSLDWINFVATSSARNRIRQWFKRSNREQNIARGRHLLERELGKTGFDALLKSERMQKVAEKLNYASVEDLLAALGYG
ETTPTLVIHRLQEVSRPAVSPPTEEESLEGSKLLLPRASVASSSVKSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRF
KGISIHHQDCPRVAQVSGERLVPVRWNEEQLRRSTQIYPVELHLEVIDRVGVLRDILSRLSDQGINVRNAKVQTFANRTA
LIDLCIDVRDKKQLTQVQSQLRQLSDVIVLRSRHHQNGSR
>Mature_759_residues
GQTAVIATSDGGLDLNSLGFEVPGWLRALLSGAEDPGDGLRPYTAEEADLLRRAFEFSYCLHQGQKRKSGDPYIAHPVAV
ATLLRELGGDAVTVAAGLLHDVIEDTEVTPEALEAEFGSEVRLLVEGVTKLSKFNFSSKTEQQAENFRRMFVAMAKDIRV
IVVKLADRLHNMRTLQYLSPSKQRQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRKIQELVNAKRAEREAE
IQAFIEELRQNLLQAGLEHFEISGRPKHLYSIYRKMQQQHKEFHEIYDLSAVRVIVHTNSECYRVLAVVHNCFRPIPGRF
KDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRVAEYGIAAHWKYKEAGSTTVKPDEERFTWLRQLLEWQNDLKD
DKEYLDTLRENLFESEVYVFTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLDTRLRNGDIVQIITHKNA
HPSLDWINFVATSSARNRIRQWFKRSNREQNIARGRHLLERELGKTGFDALLKSERMQKVAEKLNYASVEDLLAALGYGE
TTPTLVIHRLQEVSRPAVSPPTEEESLEGSKLLLPRASVASSSVKSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRFK
GISIHHQDCPRVAQVSGERLVPVRWNEEQLRRSTQIYPVELHLEVIDRVGVLRDILSRLSDQGINVRNAKVQTFANRTAL
IDLCIDVRDKKQLTQVQSQLRQLSDVIVLRSRHHQNGSR

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=138, Percent_Identity=38.4057971014493, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1790082, Length=706, Percent_Identity=42.3512747875354, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1789147, Length=716, Percent_Identity=37.5698324022346, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25143118, Length=147, Percent_Identity=36.0544217687075, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 86401; Mature: 86270

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTAVIATSDGGLDLNSLGFEVPGWLRALLSGAEDPGDGLRPYTAEEADLLRRAFEFSY
CCCEEEEEECCCCCCHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CLHQGQKRKSGDPYIAHPVAVATLLRELGGDAVTVAAGLLHDVIEDTEVTPEALEAEFGS
HHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
EVRLLVEGVTKLSKFNFSSKTEQQAENFRRMFVAMAKDIRVIVVKLADRLHNMRTLQYLS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSKQRQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRKIQELVNAKRAEREA
CCHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFIEELRQNLLQAGLEHFEISGRPKHLYSIYRKMQQQHKEFHEIYDLSAVRVIVHTN
HHHHHHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
SECYRVLAVVHNCFRPIPGRFKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRV
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHEECCCCCEEEEEEHHHHHHH
AEYGIAAHWKYKEAGSTTVKPDEERFTWLRQLLEWQNDLKDDKEYLDTLRENLFESEVYV
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
FTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLDTRLRNGDIVQIITHKN
ECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCC
AHPSLDWINFVATSSARNRIRQWFKRSNREQNIARGRHLLERELGKTGFDALLKSERMQK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAEKLNYASVEDLLAALGYGETTPTLVIHRLQEVSRPAVSPPTEEESLEGSKLLLPRASV
HHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEECCCHHH
ASSSVKSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRFKGISIHHQDCPRVAQVSGER
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHCCCCE
LVPVRWNEEQLRRSTQIYPVELHLEVIDRVGVLRDILSRLSDQGINVRNAKVQTFANRTA
EEEEECCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LIDLCIDVRDKKQLTQVQSQLRQLSDVIVLRSRHHQNGSR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GQTAVIATSDGGLDLNSLGFEVPGWLRALLSGAEDPGDGLRPYTAEEADLLRRAFEFSY
CCEEEEEECCCCCCHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
CLHQGQKRKSGDPYIAHPVAVATLLRELGGDAVTVAAGLLHDVIEDTEVTPEALEAEFGS
HHHCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
EVRLLVEGVTKLSKFNFSSKTEQQAENFRRMFVAMAKDIRVIVVKLADRLHNMRTLQYLS
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PSKQRQIAAETMEIFAPLANRLGIWHFKWELEDLAFKYLDYEAYRKIQELVNAKRAEREA
CCHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIQAFIEELRQNLLQAGLEHFEISGRPKHLYSIYRKMQQQHKEFHEIYDLSAVRVIVHTN
HHHHHHHHHHHHHHHHCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECC
SECYRVLAVVHNCFRPIPGRFKDYIGLPKANRYQSLHTAVIGLQGRPVEVQIRTEEMHRV
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHEECCCCCEEEEEEHHHHHHH
AEYGIAAHWKYKEAGSTTVKPDEERFTWLRQLLEWQNDLKDDKEYLDTLRENLFESEVYV
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEE
FTPKGDLLALPQGSCPVDFAYRIHSEIGDHCAGARVNNKIVPLDTRLRNGDIVQIITHKN
ECCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEECCC
AHPSLDWINFVATSSARNRIRQWFKRSNREQNIARGRHLLERELGKTGFDALLKSERMQK
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VAEKLNYASVEDLLAALGYGETTPTLVIHRLQEVSRPAVSPPTEEESLEGSKLLLPRASV
HHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEECCCHHH
ASSSVKSQSPILGVEGLKHQLAKCCNPLPGDPILGVVTRFKGISIHHQDCPRVAQVSGER
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHCCCCE
LVPVRWNEEQLRRSTQIYPVELHLEVIDRVGVLRDILSRLSDQGINVRNAKVQTFANRTA
EEEEECCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LIDLCIDVRDKKQLTQVQSQLRQLSDVIVLRSRHHQNGSR
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]