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The map label for this gene is ndhB

Identifier: 86608938

GI number: 86608938

Start: 1546359

End: 1547942

Strand: Reverse

Name: ndhB

Synonym: CYB_1471

Alternate gene names: 86608938

Gene position: 1547942-1546359 (Counterclockwise)

Preceding gene: 86608939

Following gene: 86608925

Centisome position: 50.81

GC content: 57.95

Gene sequence:

>1584_bases
ATGGATGTCTTTGCCCCAACCGGAAACCTGTATGCCGGTGCCATTTGGCCAGAAGTGGTGGTTACCCTCACCCTCTTGCT
CGTCTTGGTGGTGGATTTGGTCGGTGGTTCGGCGGCGCGCAAAAGTCTTCCAGCCCTCTCCATCTTTGGCTTGATCGGGG
CCTTGGTGACCTTGGTGTTGCAGTGGCAGCAGCCCCAGTTGGAGAGCTTCTTGGGCAGCTTTGCGGCGGATCCCGTCAGC
ATTCTCTTCCGTGGCCTGGTGGTGGGGACGGCGGTGCTGACGGTGATGATGGCAGAACGCTACATCGCCCAGTCGGGAGC
CCCCACAGGCGAGTTTTATGTGTTGCTGCTCACGGCCACCTTGGGGGGCATGTTCCTCTCCGGGGCGACGGACTTGATCC
TAGTGTTTGTATCTCTGGAAACCCTGGGCATCGCCAGCTACCTGATGGCCGGCTATACCAAGCGGGATCCGCGTTCTTCG
GAGGCAGCCCTGAAATATCTCTTGATCGGGGCCAGCAGCACGGCCATTTTTCTCTACGGTATGTCGCTGCTCTACGGCCT
TTCGGGTGGGCAAACGAAGCTGGAAGCCATTGCCTCCTCTATGGGGGATGCCGGCTTAGCAGGGATCATCGCTTTGGTCC
TCTGCATTGGTGGTATCGGGTTTAAGTTGGCGGCAGTGCCCTTTCACCAGTGGACTCCTGATGTCTACGAGGGATCCCCA
ACGCCGGTGGTGGCCTTTTTGTCGGTGGGATCCAAAGCGGCAGGCTTTGCCTTGGCGGTGCGCTTTCTGGCCACCGTGTT
TCCGGCCATGACGGAAGAGTGGCGGGCGGTGCTCTCGGTGCTGGCCATTTTGACCATGGTGCTGGGGAATGTGGTGGCCA
TTGCCCAAACCCGCCTCAAACGGCTACTGGCCTATTCCTCGATTGGGCAAGCGGGCTTCGTCATGATTGGTCTGGTGGTG
GGCACAGAAGCAGGCTACGCCAGCTTGATCTTCTATCTGCTGGTCTACCTGGTCATGAACCTGGGGGCCTTCTTGTGCAT
TACCCTGTTCAGCCTGAAAACCGGTACCGACGAAATTAACGAGTACAGCGGCCTTTACCAGAAGGATCCCTTCCTCACCC
TGTGCTTGAGCATTTGTTTGCTTTCGTTGGGTGGGTTGCCGCCTCTGGCGGGCTTCTTCGGCAAGTTGTACCTGTTCTGG
GCCGGTTGGCAGGCGGGGGCCTATACCCTGGTGTTGGTGGGGTTGGTCACCAGTGTTATCTCCATTTACTACTACGTGCG
GGTGGTGAAAACGATTGTGGTGAAAGAGCCGCAAGAGATGTCGGTTTCGGTGCAAAATTACCCCGAGACCAACTGGCAGG
CAGAAGGTATGCCTGCTCTGCGGGTCGGTATGGTGTTGACTCTGGTGGCCACCATCTTTGCCGGGATTCTCTCCAACCCC
TTGTTTACCTTGTCCATCCAGGCGGTGGAGCAAAGCCCGTTCTTAGGGTTCCCGACAGCACAAGCGCCCATTCCAGAGTC
GGCGAGTCTTCAGGTGGCAGTCGATGGATCTTTGGGTTCATTGACTCAGCCCAGCCAAGGGTAG

Upstream 100 bases:

>100_bases
GAGACGATTGGGAGAAGAGATGCCTGAAGCGGGGAGAGAGACCAGAAATCGCGGTATGCTGGCTGTGGATCCCCTTAATC
TCTCTTCATAGTCTTCTCCT

Downstream 100 bases:

>100_bases
TCTGTAGTTTTTCAGGGATCCACCAAGCTGACCACAATGCGTCGGTTTTGCTTGCCTTTGCTTTCGATCTTGCTCACCAC
CAACTTGCCAATTTCTCCTG

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MDVFAPTGNLYAGAIWPEVVVTLTLLLVLVVDLVGGSAARKSLPALSIFGLIGALVTLVLQWQQPQLESFLGSFAADPVS
ILFRGLVVGTAVLTVMMAERYIAQSGAPTGEFYVLLLTATLGGMFLSGATDLILVFVSLETLGIASYLMAGYTKRDPRSS
EAALKYLLIGASSTAIFLYGMSLLYGLSGGQTKLEAIASSMGDAGLAGIIALVLCIGGIGFKLAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKAAGFALAVRFLATVFPAMTEEWRAVLSVLAILTMVLGNVVAIAQTRLKRLLAYSSIGQAGFVMIGLVV
GTEAGYASLIFYLLVYLVMNLGAFLCITLFSLKTGTDEINEYSGLYQKDPFLTLCLSICLLSLGGLPPLAGFFGKLYLFW
AGWQAGAYTLVLVGLVTSVISIYYYVRVVKTIVVKEPQEMSVSVQNYPETNWQAEGMPALRVGMVLTLVATIFAGILSNP
LFTLSIQAVEQSPFLGFPTAQAPIPESASLQVAVDGSLGSLTQPSQG

Sequences:

>Translated_527_residues
MDVFAPTGNLYAGAIWPEVVVTLTLLLVLVVDLVGGSAARKSLPALSIFGLIGALVTLVLQWQQPQLESFLGSFAADPVS
ILFRGLVVGTAVLTVMMAERYIAQSGAPTGEFYVLLLTATLGGMFLSGATDLILVFVSLETLGIASYLMAGYTKRDPRSS
EAALKYLLIGASSTAIFLYGMSLLYGLSGGQTKLEAIASSMGDAGLAGIIALVLCIGGIGFKLAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKAAGFALAVRFLATVFPAMTEEWRAVLSVLAILTMVLGNVVAIAQTRLKRLLAYSSIGQAGFVMIGLVV
GTEAGYASLIFYLLVYLVMNLGAFLCITLFSLKTGTDEINEYSGLYQKDPFLTLCLSICLLSLGGLPPLAGFFGKLYLFW
AGWQAGAYTLVLVGLVTSVISIYYYVRVVKTIVVKEPQEMSVSVQNYPETNWQAEGMPALRVGMVLTLVATIFAGILSNP
LFTLSIQAVEQSPFLGFPTAQAPIPESASLQVAVDGSLGSLTQPSQG
>Mature_527_residues
MDVFAPTGNLYAGAIWPEVVVTLTLLLVLVVDLVGGSAARKSLPALSIFGLIGALVTLVLQWQQPQLESFLGSFAADPVS
ILFRGLVVGTAVLTVMMAERYIAQSGAPTGEFYVLLLTATLGGMFLSGATDLILVFVSLETLGIASYLMAGYTKRDPRSS
EAALKYLLIGASSTAIFLYGMSLLYGLSGGQTKLEAIASSMGDAGLAGIIALVLCIGGIGFKLAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKAAGFALAVRFLATVFPAMTEEWRAVLSVLAILTMVLGNVVAIAQTRLKRLLAYSSIGQAGFVMIGLVV
GTEAGYASLIFYLLVYLVMNLGAFLCITLFSLKTGTDEINEYSGLYQKDPFLTLCLSICLLSLGGLPPLAGFFGKLYLFW
AGWQAGAYTLVLVGLVTSVISIYYYVRVVKTIVVKEPQEMSVSVQNYPETNWQAEGMPALRVGMVLTLVATIFAGILSNP
LFTLSIQAVEQSPFLGFPTAQAPIPESASLQVAVDGSLGSLTQPSQG

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=434, Percent_Identity=22.1198156682028, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI251831108, Length=304, Percent_Identity=26.9736842105263, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI145693160, Length=451, Percent_Identity=33.9246119733925, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI1788614, Length=381, Percent_Identity=25.7217847769029, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788831, Length=304, Percent_Identity=26.6447368421053, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788829, Length=396, Percent_Identity=26.7676767676768, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788613, Length=367, Percent_Identity=26.7029972752044, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788827, Length=322, Percent_Identity=22.9813664596273, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NU2C_SYNJB (Q2JLH4)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477700.1
- STRING:   Q2JLH4
- GeneID:   3901470
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1471
- TIGR:   CYB_1471
- HOGENOM:   HBG747830
- OMA:   FAGWADH
- PhylomeDB:   Q2JLH4
- ProtClustDB:   PRK02504
- BioCyc:   SSP321332:CYB_1471-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.6.5.3

Molecular weight: Translated: 55672; Mature: 55672

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13b904b8)-; HASH(0x14103928)-; HASH(0x14103814)-; HASH(0x140d0ddc)-; HASH(0x14103aa8)-; HASH(0x140f7bbc)-; HASH(0x13c6225c)-; HASH(0x1410388c)-; HASH(0x140ec2a8)-; HASH(0x1410397c)-; HASH(0x140e985c)-; HASH(0x13b943a8)-; HASH(0x1317668c)-; HASH(0x140d0fbc)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFAPTGNLYAGAIWPEVVVTLTLLLVLVVDLVGGSAARKSLPALSIFGLIGALVTLVL
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHH
QWQQPQLESFLGSFAADPVSILFRGLVVGTAVLTVMMAERYIAQSGAPTGEFYVLLLTAT
HCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGGMFLSGATDLILVFVSLETLGIASYLMAGYTKRDPRSSEAALKYLLIGASSTAIFLYG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHH
MSLLYGLSGGQTKLEAIASSMGDAGLAGIIALVLCIGGIGFKLAAVPFHQWTPDVYEGSP
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCC
TPVVAFLSVGSKAAGFALAVRFLATVFPAMTEEWRAVLSVLAILTMVLGNVVAIAQTRLK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLAYSSIGQAGFVMIGLVVGTEAGYASLIFYLLVYLVMNLGAFLCITLFSLKTGTDEIN
HHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EYSGLYQKDPFLTLCLSICLLSLGGLPPLAGFFGKLYLFWAGWQAGAYTLVLVGLVTSVI
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHH
SIYYYVRVVKTIVVKEPQEMSVSVQNYPETNWQAEGMPALRVGMVLTLVATIFAGILSNP
HHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LFTLSIQAVEQSPFLGFPTAQAPIPESASLQVAVDGSLGSLTQPSQG
EEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH
>Mature Secondary Structure
MDVFAPTGNLYAGAIWPEVVVTLTLLLVLVVDLVGGSAARKSLPALSIFGLIGALVTLVL
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHH
QWQQPQLESFLGSFAADPVSILFRGLVVGTAVLTVMMAERYIAQSGAPTGEFYVLLLTAT
HCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LGGMFLSGATDLILVFVSLETLGIASYLMAGYTKRDPRSSEAALKYLLIGASSTAIFLYG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEECCCCHHHHHHH
MSLLYGLSGGQTKLEAIASSMGDAGLAGIIALVLCIGGIGFKLAAVPFHQWTPDVYEGSP
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCC
TPVVAFLSVGSKAAGFALAVRFLATVFPAMTEEWRAVLSVLAILTMVLGNVVAIAQTRLK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLAYSSIGQAGFVMIGLVVGTEAGYASLIFYLLVYLVMNLGAFLCITLFSLKTGTDEIN
HHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
EYSGLYQKDPFLTLCLSICLLSLGGLPPLAGFFGKLYLFWAGWQAGAYTLVLVGLVTSVI
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHH
SIYYYVRVVKTIVVKEPQEMSVSVQNYPETNWQAEGMPALRVGMVLTLVATIFAGILSNP
HHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LFTLSIQAVEQSPFLGFPTAQAPIPESASLQVAVDGSLGSLTQPSQG
EEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA