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The map label for this gene is pcrA [H]

Identifier: 86608842

GI number: 86608842

Start: 1437380

End: 1439743

Strand: Reverse

Name: pcrA [H]

Synonym: CYB_1368

Alternate gene names: 86608842

Gene position: 1439743-1437380 (Counterclockwise)

Preceding gene: 86608845

Following gene: 86608840

Centisome position: 47.26

GC content: 52.66

Gene sequence:

>2364_bases
ATGACTCACGCCTCTTTGCTGGATGCCCTCAACCCTTCTCAACGGCAAGCGGTGCAACACTTTTGTGGCCCGCTGCTGGT
GGTGGCGGGGGCAGGTTCCGGCAAGACGCGCGCCCTCACCCACCGCATTGCCTACCTGGTTCGCCACTATCGGGTGGATC
CAGGAGAAATTTTGGCCGTGACCTTCACCAACAAGGCCGCCCGTGAGATGAAAGAGCGCATTGAGCAGCTCTTTGCCGAA
CAAGAAGCTCAAGAACAATTTGGGATCCCCTTAGAGGAATTGGAGCCGGCCCAGGCAACGCGACTAAAATCTGCTGTTTA
CCACCGCTGGATTAAGCCTCTTTGGATTGGCACGTTCCACAGCTTATTTGCCCAGATCCTGCGCCTGGAGATCGAAAAAT
ATCAGGATCCCAAGGGGCGCAAGTGGACTCGCCACTTCTCGATCTTCGATGAATCAGATGCCCAATCTCTGGTGAAGGAG
ATTGTAACCAAGCAACTGAACTTGGATGAGCGCAAGTTTGACCCAAGGTCGGTACGCTACGCCATTAGCAATGCCAAAAA
CCAAGGTTGGACTCCTGCCGACCTGGAGCGCAATCAGCCCAACTTCCGTGGCCGTACCATTGCCCAAGTTTACGAAATCT
ATGAAGACCAACTGGCAGCCAACAATGCTCTGGACTTTGATAACTTGATTTGGATACCAGTGCAGCTTTTTCGACAAAAC
GAGCAGGTGTTGGCTTACTGGCACCAACGATTTCGACACATTTTGGTGGATGAATACCAAGACACCAACCGCACTCAATA
TGAGCTAATCCGTCTGCTGGCTACCAACGGCGAGACCCGCAGATCTCAGTTGGACTGGAGAAACCGATCCATCTTTGTGG
TGGGAGATGCGGATCAAAGCATCTATCGCTTTCGCGGGGCAGATTTTACCATCTTGCTGGAGTTTCAAGAGACCTTTGGC
GATGGTCTCCCCGATGACGATACCCGCACCCTGATCAAGCTGGAGGAGAACTATCGCTCTACAGCTACCATTCTGCAGGC
AGCTAACGCTCTCATCGAGAAAAATACAGAGCGCATTGATAAAGTTCTCAGGCCAACCCGTCAGCAAGGAAATCCGATTT
TTCTTCATCAGGCGGATGACGAGATTGCAGAGGCGGAGTTTGTCGTCAATACCATCCGACGATTACACGGCCAAAATGCC
AATGCTTTTTCCTGGGGAGATTTTGCCATTCTCTACCGCATCAACGCTCAATCCCGTCCTTTTGAAGAGGTCTTGCTGCG
GTGGGGGATCCCTTACACGGTGGTAGGCGGACTGCGATTTTACGATCGCAAAGAAATTAAGGATGTCCTGGCCTATTTGC
GGGTTGTGGCCAACCCTGCCGACTCCTTTAGCCTGCTGCGAATTATTAACGTGCCGCGCCGTGGCATCGGCAAATCCACC
CTGGACAAGCTGACCGAAGCGGCGGCTGCTCTAAACGTGCCTTTGTGGGCAATTCTCAGCGACGAAACCTCTGTAAGTAG
CTTGGCGGGCCGTTCGGCTAAGCCTGTCATTCAGTTTGCTCAGTTGATTCAGCATTGGCATCGACAGGCTTCTCAGACTT
CAGCCGCCACCCTTATCGAAGGGTTGCTAAGGGATTCCGGCTATTTGGAGGATCTCAAGGCACAGGGTACAGACGAAGCC
GAAGAAAGAGCGGCCAACGTGATGGAACTTTACAATGCCGCCCGCCAATTCGAGGAAGAACAGGAGGATCCCAGCCTTGA
GGCTTTTTTGAGCAATGTGTCTTTGGCTTCCGACTTGGACAACCTTCAGGAAGGCGCTGAGAAAGTTTCGCTAATGACGT
TGCACTCTTCCAAAGGTTTGGAGTTTCCTGTTGTGTTCTTGGTGGGGGTGGAACAGGGTCTATTTCCCAATTTCCGGGCT
TTGGAAGATCCCGCTGCTTTGGAAGAAGAGCGACGCCTTTGCTACGTGGGCATTACCCGTGCCCGTGAGCAGTTGTATAT
CAGCCACGCCAGGGAAAGGATGCTCTACGGCAACCGCGAACCAGCTATTCCTTCGGTATTTTTAAGTGAACTGCCCCAAG
AGTTAATAATCGAATTCGACGGACGCGGAGCCGTTTCTCGACAGCTCTCCCACGGCAGTGGACTGTCCCTTTCAGATGCA
CCCAAGGCCAGGCCCAATCGCCGCCAAAGCCGTTCAGAACAGTGGGCGATTGGAGATCGTTTGCAACATCCCCAATTCGG
ACAAGGCCAGATTACCCATGTGTTCGGCAGTGGTGAACGGGTCACGATTGCGGTGAGGTTTCCCGCTATCGGTCAACAGA
AAATCTTGGATCCCCGTATCGATCCTATTCAAAAGCTTAGCTAG

Upstream 100 bases:

>100_bases
TTAACCGATCTTCATCTTTGGTGCTCCAAAATGCCCTCTGGGCTTCTCATCCCCATTGGGTTGGTGCCCATAATGGAAGG
CAGTTTCTGGTTTGCTGGCC

Downstream 100 bases:

>100_bases
GCGGCTGAACTCGGAAGGGCTCCAGCGATGAAGAGAGGCAAAGGGAAGTGCTGTAGAAGTCCGTAGCTCATTCGAGATTG
CCTTGCCGTTGACGTCGTTG

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 787; Mature: 786

Protein sequence:

>787_residues
MTHASLLDALNPSQRQAVQHFCGPLLVVAGAGSGKTRALTHRIAYLVRHYRVDPGEILAVTFTNKAAREMKERIEQLFAE
QEAQEQFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQILRLEIEKYQDPKGRKWTRHFSIFDESDAQSLVKE
IVTKQLNLDERKFDPRSVRYAISNAKNQGWTPADLERNQPNFRGRTIAQVYEIYEDQLAANNALDFDNLIWIPVQLFRQN
EQVLAYWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSQLDWRNRSIFVVGDADQSIYRFRGADFTILLEFQETFG
DGLPDDDTRTLIKLEENYRSTATILQAANALIEKNTERIDKVLRPTRQQGNPIFLHQADDEIAEAEFVVNTIRRLHGQNA
NAFSWGDFAILYRINAQSRPFEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVANPADSFSLLRIINVPRRGIGKST
LDKLTEAAAALNVPLWAILSDETSVSSLAGRSAKPVIQFAQLIQHWHRQASQTSAATLIEGLLRDSGYLEDLKAQGTDEA
EERAANVMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDNLQEGAEKVSLMTLHSSKGLEFPVVFLVGVEQGLFPNFRA
LEDPAALEEERRLCYVGITRAREQLYISHARERMLYGNREPAIPSVFLSELPQELIIEFDGRGAVSRQLSHGSGLSLSDA
PKARPNRRQSRSEQWAIGDRLQHPQFGQGQITHVFGSGERVTIAVRFPAIGQQKILDPRIDPIQKLS

Sequences:

>Translated_787_residues
MTHASLLDALNPSQRQAVQHFCGPLLVVAGAGSGKTRALTHRIAYLVRHYRVDPGEILAVTFTNKAAREMKERIEQLFAE
QEAQEQFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQILRLEIEKYQDPKGRKWTRHFSIFDESDAQSLVKE
IVTKQLNLDERKFDPRSVRYAISNAKNQGWTPADLERNQPNFRGRTIAQVYEIYEDQLAANNALDFDNLIWIPVQLFRQN
EQVLAYWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSQLDWRNRSIFVVGDADQSIYRFRGADFTILLEFQETFG
DGLPDDDTRTLIKLEENYRSTATILQAANALIEKNTERIDKVLRPTRQQGNPIFLHQADDEIAEAEFVVNTIRRLHGQNA
NAFSWGDFAILYRINAQSRPFEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVANPADSFSLLRIINVPRRGIGKST
LDKLTEAAAALNVPLWAILSDETSVSSLAGRSAKPVIQFAQLIQHWHRQASQTSAATLIEGLLRDSGYLEDLKAQGTDEA
EERAANVMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDNLQEGAEKVSLMTLHSSKGLEFPVVFLVGVEQGLFPNFRA
LEDPAALEEERRLCYVGITRAREQLYISHARERMLYGNREPAIPSVFLSELPQELIIEFDGRGAVSRQLSHGSGLSLSDA
PKARPNRRQSRSEQWAIGDRLQHPQFGQGQITHVFGSGERVTIAVRFPAIGQQKILDPRIDPIQKLS
>Mature_786_residues
THASLLDALNPSQRQAVQHFCGPLLVVAGAGSGKTRALTHRIAYLVRHYRVDPGEILAVTFTNKAAREMKERIEQLFAEQ
EAQEQFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQILRLEIEKYQDPKGRKWTRHFSIFDESDAQSLVKEI
VTKQLNLDERKFDPRSVRYAISNAKNQGWTPADLERNQPNFRGRTIAQVYEIYEDQLAANNALDFDNLIWIPVQLFRQNE
QVLAYWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSQLDWRNRSIFVVGDADQSIYRFRGADFTILLEFQETFGD
GLPDDDTRTLIKLEENYRSTATILQAANALIEKNTERIDKVLRPTRQQGNPIFLHQADDEIAEAEFVVNTIRRLHGQNAN
AFSWGDFAILYRINAQSRPFEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVANPADSFSLLRIINVPRRGIGKSTL
DKLTEAAAALNVPLWAILSDETSVSSLAGRSAKPVIQFAQLIQHWHRQASQTSAATLIEGLLRDSGYLEDLKAQGTDEAE
ERAANVMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDNLQEGAEKVSLMTLHSSKGLEFPVVFLVGVEQGLFPNFRAL
EDPAALEEERRLCYVGITRAREQLYISHARERMLYGNREPAIPSVFLSELPQELIIEFDGRGAVSRQLSHGSGLSLSDAP
KARPNRRQSRSEQWAIGDRLQHPQFGQGQITHVFGSGERVTIAVRFPAIGQQKILDPRIDPIQKLS

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=777, Percent_Identity=37.7091377091377, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI48994965, Length=706, Percent_Identity=38.6685552407932, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1787196, Length=371, Percent_Identity=28.8409703504043, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=784, Percent_Identity=27.9336734693878, Blast_Score=220, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6324477, Length=482, Percent_Identity=25.103734439834, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 89722; Mature: 89591

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHASLLDALNPSQRQAVQHFCGPLLVVAGAGSGKTRALTHRIAYLVRHYRVDPGEILAV
CCHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TFTNKAAREMKERIEQLFAEQEAQEQFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFH
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLFAQILRLEIEKYQDPKGRKWTRHFSIFDESDAQSLVKEIVTKQLNLDERKFDPRSVRY
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHH
AISNAKNQGWTPADLERNQPNFRGRTIAQVYEIYEDQLAANNALDFDNLIWIPVQLFRQN
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCC
EQVLAYWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSQLDWRNRSIFVVGDADQS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCH
IYRFRGADFTILLEFQETFGDGLPDDDTRTLIKLEENYRSTATILQAANALIEKNTERID
HHHCCCCCEEEEEEEHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHH
KVLRPTRQQGNPIFLHQADDEIAEAEFVVNTIRRLHGQNANAFSWGDFAILYRINAQSRP
HHHCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC
FEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVANPADSFSLLRIINVPRRGIGKST
HHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
LDKLTEAAAALNVPLWAILSDETSVSSLAGRSAKPVIQFAQLIQHWHRQASQTSAATLIE
HHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLRDSGYLEDLKAQGTDEAEERAANVMELYNAARQFEEEQEDPSLEAFLSNVSLASDLD
HHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEGAEKVSLMTLHSSKGLEFPVVFLVGVEQGLFPNFRALEDPAALEEERRLCYVGITR
HHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEHHH
AREQLYISHARERMLYGNREPAIPSVFLSELPQELIIEFDGRGAVSRQLSHGSGLSLSDA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHEEEECCCCHHHHHHCCCCCCCCCCC
PKARPNRRQSRSEQWAIGDRLQHPQFGQGQITHVFGSGERVTIAVRFPAIGQQKILDPRI
CCCCCCHHHCCCCCCCCHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHCCCCCC
DPIQKLS
CHHHHCC
>Mature Secondary Structure 
THASLLDALNPSQRQAVQHFCGPLLVVAGAGSGKTRALTHRIAYLVRHYRVDPGEILAV
CHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TFTNKAAREMKERIEQLFAEQEAQEQFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFH
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLFAQILRLEIEKYQDPKGRKWTRHFSIFDESDAQSLVKEIVTKQLNLDERKFDPRSVRY
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHH
AISNAKNQGWTPADLERNQPNFRGRTIAQVYEIYEDQLAANNALDFDNLIWIPVQLFRQN
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCC
EQVLAYWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSQLDWRNRSIFVVGDADQS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCH
IYRFRGADFTILLEFQETFGDGLPDDDTRTLIKLEENYRSTATILQAANALIEKNTERID
HHHCCCCCEEEEEEEHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHH
KVLRPTRQQGNPIFLHQADDEIAEAEFVVNTIRRLHGQNANAFSWGDFAILYRINAQSRP
HHHCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC
FEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVANPADSFSLLRIINVPRRGIGKST
HHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHH
LDKLTEAAAALNVPLWAILSDETSVSSLAGRSAKPVIQFAQLIQHWHRQASQTSAATLIE
HHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLRDSGYLEDLKAQGTDEAEERAANVMELYNAARQFEEEQEDPSLEAFLSNVSLASDLD
HHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEGAEKVSLMTLHSSKGLEFPVVFLVGVEQGLFPNFRALEDPAALEEERRLCYVGITR
HHHHHHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEHHH
AREQLYISHARERMLYGNREPAIPSVFLSELPQELIIEFDGRGAVSRQLSHGSGLSLSDA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHEEEECCCCHHHHHHCCCCCCCCCCC
PKARPNRRQSRSEQWAIGDRLQHPQFGQGQITHVFGSGERVTIAVRFPAIGQQKILDPRI
CCCCCCHHHCCCCCCCCHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHCCCCCC
DPIQKLS
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]