Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 86608775

GI number: 86608775

Start: 1362410

End: 1363360

Strand: Reverse

Name: potB [H]

Synonym: CYB_1299

Alternate gene names: 86608775

Gene position: 1363360-1362410 (Counterclockwise)

Preceding gene: 86608776

Following gene: 86608773

Centisome position: 44.75

GC content: 60.67

Gene sequence:

>951_bases
ATGTTGAAGCTGCCATCTCCTGTGGGCAAGTGGATCCCTGGCCAAGCCCAGGTGCGATTTGGGGAGCTGCTCACCCCTTG
GCAGGAGCTGGGACGCAGCCTGATTTTTGCTGGGCCAGGGGTGCTGTGGGTGATGTTTTTTCTGGTGCTGCCGGGGCTGT
TGCTGTTGGTGTGCAGCTTTCTCAGCCGCGGGGAGTTTGGCCAGATTCAACTGCCCCTGACCCTGGAGAACTATCTGCGC
ATTCTCGGCTTTGGCCCCTTGGGATGGACGCCCATCTACTGGCAGATCTTGGGCCGCAGCCTGCTGGTGGCGGGGGTGAC
CACCTTTTTTTGCGTGCTGCTGGCCTACCCATTGGCTTTCTTCATTGCCGCCCACCCACCCCGACAACGGGATCTGTTGC
TCACCTTGGTGGTGGTGCCCTTTTGGACGAACCTGGTGATCCGCACCTACGCCTGGATGGTGGTGCTGGCCCCGGAAGGG
TGGCCGGCTCGCTTGGCGGCCTGGCTGGGCTGGATCCCACCGCAAACGGCTCTGTATCCCAGTTGGCTGGCGGTTTATCT
GGGCATGGTCTACACGTTTTTGCCCTACATGGTCTTGCCCCTGTACACGGCGGTGGAGCGCATCGATTGGCGGCTGGCGG
AAGCGGCACGGGATCTCTATGCCAAGGGTTGGCAGGTCTTTTGGTGGGTGCTTCTGCCCCAGACCTTGCCTGGACTGGCG
GCGGGCCTCATTCTGGTGGGGATCCCGGCTTTCGGCATGTTCGTAGTGCCGGATTTGTTGGGGGGCAGCAAAACGCTGTT
GATCGGGAATGTGATTCAGTTGCAGTTTGGCTCCAGCCTGGACTATCCTTTCGGGGCGGCCCTCAGCTTTTTGGTTACCG
GCCTCACCCTGGGGTTGATCTACGCCTACAGCCGGTATGTTGGCACCCGCAGCCTAGAACATCTGGTTTGA

Upstream 100 bases:

>100_bases
TTGAGGATGCAGGGTTTGAGATCGGCGCAGCGCTGGTGGCCCACCTACCCCCGAAAAGCCTAATCCGTTTGCGACCGGAT
CCCCGTCAGGAGGAGACTGG

Downstream 100 bases:

>100_bases
GGAGCTGCTGTGGGCCTTGAGCAAAGCCGCCAGTTGCTTGCGCATCAGGATCCCGGGCTTATCGGTGAGCATGTGCTGCT
CGGCGCTGAGGGTGAGGGGC

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLKLPSPVGKWIPGQAQVRFGELLTPWQELGRSLIFAGPGVLWVMFFLVLPGLLLLVCSFLSRGEFGQIQLPLTLENYLR
ILGFGPLGWTPIYWQILGRSLLVAGVTTFFCVLLAYPLAFFIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEG
WPARLAAWLGWIPPQTALYPSWLAVYLGMVYTFLPYMVLPLYTAVERIDWRLAEAARDLYAKGWQVFWWVLLPQTLPGLA
AGLILVGIPAFGMFVVPDLLGGSKTLLIGNVIQLQFGSSLDYPFGAALSFLVTGLTLGLIYAYSRYVGTRSLEHLV

Sequences:

>Translated_316_residues
MLKLPSPVGKWIPGQAQVRFGELLTPWQELGRSLIFAGPGVLWVMFFLVLPGLLLLVCSFLSRGEFGQIQLPLTLENYLR
ILGFGPLGWTPIYWQILGRSLLVAGVTTFFCVLLAYPLAFFIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEG
WPARLAAWLGWIPPQTALYPSWLAVYLGMVYTFLPYMVLPLYTAVERIDWRLAEAARDLYAKGWQVFWWVLLPQTLPGLA
AGLILVGIPAFGMFVVPDLLGGSKTLLIGNVIQLQFGSSLDYPFGAALSFLVTGLTLGLIYAYSRYVGTRSLEHLV
>Mature_316_residues
MLKLPSPVGKWIPGQAQVRFGELLTPWQELGRSLIFAGPGVLWVMFFLVLPGLLLLVCSFLSRGEFGQIQLPLTLENYLR
ILGFGPLGWTPIYWQILGRSLLVAGVTTFFCVLLAYPLAFFIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEG
WPARLAAWLGWIPPQTALYPSWLAVYLGMVYTFLPYMVLPLYTAVERIDWRLAEAARDLYAKGWQVFWWVLLPQTLPGLA
AGLILVGIPAFGMFVVPDLLGGSKTLLIGNVIQLQFGSSLDYPFGAALSFLVTGLTLGLIYAYSRYVGTRSLEHLV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=273, Percent_Identity=42.8571428571429, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1787080, Length=248, Percent_Identity=39.5161290322581, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1787713, Length=269, Percent_Identity=31.5985130111524, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35303; Mature: 35303

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLPSPVGKWIPGQAQVRFGELLTPWQELGRSLIFAGPGVLWVMFFLVLPGLLLLVCSF
CCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
LSRGEFGQIQLPLTLENYLRILGFGPLGWTPIYWQILGRSLLVAGVTTFFCVLLAYPLAF
HCCCCCCEEEECEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEGWPARLAAWLGWIPPQTALYP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCHHHHH
SWLAVYLGMVYTFLPYMVLPLYTAVERIDWRLAEAARDLYAKGWQVFWWVLLPQTLPGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AGLILVGIPAFGMFVVPDLLGGSKTLLIGNVIQLQFGSSLDYPFGAALSFLVTGLTLGLI
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
YAYSRYVGTRSLEHLV
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKLPSPVGKWIPGQAQVRFGELLTPWQELGRSLIFAGPGVLWVMFFLVLPGLLLLVCSF
CCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
LSRGEFGQIQLPLTLENYLRILGFGPLGWTPIYWQILGRSLLVAGVTTFFCVLLAYPLAF
HCCCCCCEEEECEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIAAHPPRQRDLLLTLVVVPFWTNLVIRTYAWMVVLAPEGWPARLAAWLGWIPPQTALYP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCCCCCHHHHH
SWLAVYLGMVYTFLPYMVLPLYTAVERIDWRLAEAARDLYAKGWQVFWWVLLPQTLPGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AGLILVGIPAFGMFVVPDLLGGSKTLLIGNVIQLQFGSSLDYPFGAALSFLVTGLTLGLI
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
YAYSRYVGTRSLEHLV
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]