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The map label for this gene is walK [H]

Identifier: 86608725

GI number: 86608725

Start: 1296879

End: 1297727

Strand: Reverse

Name: walK [H]

Synonym: CYB_1249

Alternate gene names: 86608725

Gene position: 1297727-1296879 (Counterclockwise)

Preceding gene: 86608726

Following gene: 86608724

Centisome position: 42.59

GC content: 54.53

Gene sequence:

>849_bases
ATGTTCAGGAGAGTTCAGCGTGAATGTATCAGTTTTAGCGATTGGGAAATCGATTTGGCCCAGCAAATGGCGGCCCAGTG
CGCGATTGCCCTGCGACAGGCCAGGCTCTACCTCAGCAGCCAGGCTCAGGTGACGGAATTGAAAAAACTCAGCCAAATTA
GGGATAACTTCCTGGCCGCCGTCTCCCATGAGCTGCGCACTCCCCTTACCAGCATTAGCCTCTCGGTTCACCTGCTGAAA
CTGAGCTATCAAGTTTCTCCTCTCACCCCCAAGCAAATCCAGTATCTTGAGGTTCTGGAGCAGGCGTGCGAGCGAGAGAT
CTCCCTAATTAACGATCTTCTGGATCTACAAAATTTGTCGGGCAATACGCTCAAGCTGCCCTCTGAGCAGGTTAACCTGC
CTCTCCTTTTGGCACCTCTTATCAATCGGTTCCAAGACCGGATGCGCAAGCGGCAGCAAACCCTAGAAGTTCACTGGGAT
CCCCACCTGCCCTATCTGCACACCCATCCCCGCCTGTTGGAACGCTTGGTCTGTGAGCTTTTGACCAATGCCCACAAATT
CACGCCCGCCGGCAAACACATTGTCCTAGAGGTTGTTGCTTTGGGTTCCGACCGCTGGGAGCTGCGCTTGACCAACACGG
GCACCACCATTCCCCTCGAAGAACAAGAACGCATTTTCGAGCAGTTTTATCGCATCCCCCACTCGGATCCCTGGCAGCAG
GGGGGAACAGGCTTGGGATTGGCTCTGGCCCAAGCCATTGCCAACCACTTGGGCGGCTCTTTGCAGGTGGAAAGCACTGC
CCACCAAACCACGTTTATTCTTCGCTTGCCCGCAGCAGAATCTTGTTGA

Upstream 100 bases:

>100_bases
GTATTCCTCAGGGCTTTGCGGGATACCGCCTTCCTGCTTGGACTCCGTCCCGCTGACGCGAACAGAGATAGGCAGGATGC
GATCGCTTCTTTCCTGAACC

Downstream 100 bases:

>100_bases
CGATTCGGTTTGGGCCACTTATGGTGAGATTTACCATATGGGGGGCAGGGTGAAGGTAGCGCTGTTGCAACTGAACTACA
TCGTCGGCGATCTAAGCGGA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MFRRVQRECISFSDWEIDLAQQMAAQCAIALRQARLYLSSQAQVTELKKLSQIRDNFLAAVSHELRTPLTSISLSVHLLK
LSYQVSPLTPKQIQYLEVLEQACEREISLINDLLDLQNLSGNTLKLPSEQVNLPLLLAPLINRFQDRMRKRQQTLEVHWD
PHLPYLHTHPRLLERLVCELLTNAHKFTPAGKHIVLEVVALGSDRWELRLTNTGTTIPLEEQERIFEQFYRIPHSDPWQQ
GGTGLGLALAQAIANHLGGSLQVESTAHQTTFILRLPAAESC

Sequences:

>Translated_282_residues
MFRRVQRECISFSDWEIDLAQQMAAQCAIALRQARLYLSSQAQVTELKKLSQIRDNFLAAVSHELRTPLTSISLSVHLLK
LSYQVSPLTPKQIQYLEVLEQACEREISLINDLLDLQNLSGNTLKLPSEQVNLPLLLAPLINRFQDRMRKRQQTLEVHWD
PHLPYLHTHPRLLERLVCELLTNAHKFTPAGKHIVLEVVALGSDRWELRLTNTGTTIPLEEQERIFEQFYRIPHSDPWQQ
GGTGLGLALAQAIANHLGGSLQVESTAHQTTFILRLPAAESC
>Mature_282_residues
MFRRVQRECISFSDWEIDLAQQMAAQCAIALRQARLYLSSQAQVTELKKLSQIRDNFLAAVSHELRTPLTSISLSVHLLK
LSYQVSPLTPKQIQYLEVLEQACEREISLINDLLDLQNLSGNTLKLPSEQVNLPLLLAPLINRFQDRMRKRQQTLEVHWD
PHLPYLHTHPRLLERLVCELLTNAHKFTPAGKHIVLEVVALGSDRWELRLTNTGTTIPLEEQERIFEQFYRIPHSDPWQQ
GGTGLGLALAQAIANHLGGSLQVESTAHQTTFILRLPAAESC

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=30.3964757709251, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=30.4526748971193, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=29.4117647058824, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081816, Length=269, Percent_Identity=30.1115241635688, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=30, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786783, Length=279, Percent_Identity=27.5985663082437, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790346, Length=218, Percent_Identity=30.7339449541284, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=28.82096069869, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=223, Percent_Identity=27.3542600896861, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788713, Length=235, Percent_Identity=25.531914893617, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788393, Length=237, Percent_Identity=27.8481012658228, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787894, Length=241, Percent_Identity=27.3858921161826, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=27.1493212669683, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 32101; Mature: 32101

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRVQRECISFSDWEIDLAQQMAAQCAIALRQARLYLSSQAQVTELKKLSQIRDNFLAA
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSHELRTPLTSISLSVHLLKLSYQVSPLTPKQIQYLEVLEQACEREISLINDLLDLQNLS
HHHHHHCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNTLKLPSEQVNLPLLLAPLINRFQDRMRKRQQTLEVHWDPHLPYLHTHPRLLERLVCEL
CCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHH
LTNAHKFTPAGKHIVLEVVALGSDRWELRLTNTGTTIPLEEQERIFEQFYRIPHSDPWQQ
HHCCHHCCCCCCCEEEEHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
GGTGLGLALAQAIANHLGGSLQVESTAHQTTFILRLPAAESC
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MFRRVQRECISFSDWEIDLAQQMAAQCAIALRQARLYLSSQAQVTELKKLSQIRDNFLAA
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VSHELRTPLTSISLSVHLLKLSYQVSPLTPKQIQYLEVLEQACEREISLINDLLDLQNLS
HHHHHHCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNTLKLPSEQVNLPLLLAPLINRFQDRMRKRQQTLEVHWDPHLPYLHTHPRLLERLVCEL
CCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHH
LTNAHKFTPAGKHIVLEVVALGSDRWELRLTNTGTTIPLEEQERIFEQFYRIPHSDPWQQ
HHCCHHCCCCCCCEEEEHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
GGTGLGLALAQAIANHLGGSLQVESTAHQTTFILRLPAAESC
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA