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The map label for this gene is nadE [H]

Identifier: 86608724

GI number: 86608724

Start: 1295153

End: 1296829

Strand: Reverse

Name: nadE [H]

Synonym: CYB_1248

Alternate gene names: 86608724

Gene position: 1296829-1295153 (Counterclockwise)

Preceding gene: 86608725

Following gene: 86608721

Centisome position: 42.57

GC content: 61.6

Gene sequence:

>1677_bases
GTGAAGGTAGCGCTGTTGCAACTGAACTACATCGTCGGCGATCTAAGCGGAAATGCCGAGCGCATCCGTTCGGCAGTCGA
GGCAGTGGCGGCGGCGGGAGCAGAGTTGGCCATCACCTCCGAGCTGGCGTTGCTGGGATATCCACCCCGCGATTTGTTGC
TCTATCCGGCCCTGGTCGAGAGGGCAAGCCAGGTTTTGCAGCACTTGGCCAGCCGGTTACAGGCCAGCATTCCGGTAGTC
GTGGGATCCGTGCAGCCCAATCCTCTGCCTGAGGGTCGGCCCCTTTACAATGCTGCTGCCTGGCTGGAGGGAGGCCAGAT
CCGCCACTGGTTTCAGAAGTCGCTCTTGCCCACCTACGATGTCTTCGACGAAGATCGCTATTTTGAGCCGGGATCCCAGC
CCCAACCCATCGAGTACCGTGGCCGCCGCCTGGGCTTTACCATTTGTGAAGACATTTGGAACGACCGCGACTTTTGGCAG
CACCGCCGCTACCACTGGGATCCGGTGGAACAGATGGCCCGCTGCGGTGCCGACCTGCTGATTAACCTCTCGGCTTCCCC
CTTCAGCCTAGGCAAACAGAAGTTGCGGGTGGAGATGCTGGGATCCCTGGCCCGCAAGCACGCCCTGCCCATTCTCTACG
TCAATCAGGTGGGGGGCAATGATGATTTGATCTTCGATGGGGCCAGTTGTGCCGTGGATCGCCGGGGTCGGGTTGTGGCC
CAAGCGGCTGCCTTTCAGCCGGATCAGCTCATCCTGGATGTGGACGAGTTGACGCAGTCTCCCAACCCAGACCAGAGCCC
ACCGACCGCGGGATCCCGCTGGCCGACCGAGCCGGAAGCTGAGCTGTACGAGGCGTTGGTTTTGGGCACCCGCGATTACG
TCAGCAAATGTGGCTTTCGCGAGGTGCTATTGGGCCTGTCGGGAGGGATCGATTCTGCCGTCACTGCTGTGATTGCTGCC
GATGCTTTGGGGCCGGAGCGGGTGCTGGGGGTGCTCATGCCCTCTCCCTATTCCAGCCCTGGCAGCCTGACCGACGCCTA
CCAACTGGCGCAAAACCTGGGGATCCCGACCCTGAAGCTGCCGATTCAGTCCATCATGGAGAGCTACCACCAGCTTTTGG
CAGAGCCCTTTGCCGGCCTCCCCCCAGACATCACGGAAGAAAACCTGCAATCTCGCATTCGCGGCACCCTCCTCATGGCC
CTTTCCAACAAGTTCGGGCGGCTGCTGCTGACCACAGGCAACAAATCAGAGCTAGCAGTGGGCTACTGCACCATTTACGG
GGATATGTCCGGCGGCCTGGCGGTGATCTCCGATGTGCCCAAAACCTGGGTGTACCGCTTGGCCCGCTGGATCAACCGAC
AGCAGGAGCGGATCCCGCTTGCTACTCTGACCAAGCCCCCCTCGGCAGAATTGCGACCCGGCCAACAAGATAGCGACAGC
TTGCCCCCCTACGATTTGCTGGACAGGATCCTGCAGCGGCACATCGAGCAGCATCAATCCGGCCCCGAGTTGGTGGCTCA
AGGGTTCGATCCCGACACGGTGCAGCACGTGCTGCGGCTGGTTCAGAATGCCGAGTTCAAGCGACACCAAGCTCCCCCTG
GGCTGCGCATCACCGATCGCGCCTTCGGCACCGGCTGGCGGATGCCAATCGCCAAGCGGCAGGAAGGGATCAGTTGA

Upstream 100 bases:

>100_bases
GCCCACCAAACCACGTTTATTCTTCGCTTGCCCGCAGCAGAATCTTGTTGACGATTCGGTTTGGGCCACTTATGGTGAGA
TTTACCATATGGGGGGCAGG

Downstream 100 bases:

>100_bases
AACCCTCATCTCAATTCAAACCAGGACACCGCACCCACACCTGAAACATTAGAATGCACGGGGCCAGAAGGCTTTTCAAG
GACTCCGTCCCGCTGGCGCG

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MKVALLQLNYIVGDLSGNAERIRSAVEAVAAAGAELAITSELALLGYPPRDLLLYPALVERASQVLQHLASRLQASIPVV
VGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYDVFDEDRYFEPGSQPQPIEYRGRRLGFTICEDIWNDRDFWQ
HRRYHWDPVEQMARCGADLLINLSASPFSLGKQKLRVEMLGSLARKHALPILYVNQVGGNDDLIFDGASCAVDRRGRVVA
QAAAFQPDQLILDVDELTQSPNPDQSPPTAGSRWPTEPEAELYEALVLGTRDYVSKCGFREVLLGLSGGIDSAVTAVIAA
DALGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPIQSIMESYHQLLAEPFAGLPPDITEENLQSRIRGTLLMA
LSNKFGRLLLTTGNKSELAVGYCTIYGDMSGGLAVISDVPKTWVYRLARWINRQQERIPLATLTKPPSAELRPGQQDSDS
LPPYDLLDRILQRHIEQHQSGPELVAQGFDPDTVQHVLRLVQNAEFKRHQAPPGLRITDRAFGTGWRMPIAKRQEGIS

Sequences:

>Translated_558_residues
MKVALLQLNYIVGDLSGNAERIRSAVEAVAAAGAELAITSELALLGYPPRDLLLYPALVERASQVLQHLASRLQASIPVV
VGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYDVFDEDRYFEPGSQPQPIEYRGRRLGFTICEDIWNDRDFWQ
HRRYHWDPVEQMARCGADLLINLSASPFSLGKQKLRVEMLGSLARKHALPILYVNQVGGNDDLIFDGASCAVDRRGRVVA
QAAAFQPDQLILDVDELTQSPNPDQSPPTAGSRWPTEPEAELYEALVLGTRDYVSKCGFREVLLGLSGGIDSAVTAVIAA
DALGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPIQSIMESYHQLLAEPFAGLPPDITEENLQSRIRGTLLMA
LSNKFGRLLLTTGNKSELAVGYCTIYGDMSGGLAVISDVPKTWVYRLARWINRQQERIPLATLTKPPSAELRPGQQDSDS
LPPYDLLDRILQRHIEQHQSGPELVAQGFDPDTVQHVLRLVQNAEFKRHQAPPGLRITDRAFGTGWRMPIAKRQEGIS
>Mature_558_residues
MKVALLQLNYIVGDLSGNAERIRSAVEAVAAAGAELAITSELALLGYPPRDLLLYPALVERASQVLQHLASRLQASIPVV
VGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYDVFDEDRYFEPGSQPQPIEYRGRRLGFTICEDIWNDRDFWQ
HRRYHWDPVEQMARCGADLLINLSASPFSLGKQKLRVEMLGSLARKHALPILYVNQVGGNDDLIFDGASCAVDRRGRVVA
QAAAFQPDQLILDVDELTQSPNPDQSPPTAGSRWPTEPEAELYEALVLGTRDYVSKCGFREVLLGLSGGIDSAVTAVIAA
DALGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPIQSIMESYHQLLAEPFAGLPPDITEENLQSRIRGTLLMA
LSNKFGRLLLTTGNKSELAVGYCTIYGDMSGGLAVISDVPKTWVYRLARWINRQQERIPLATLTKPPSAELRPGQQDSDS
LPPYDLLDRILQRHIEQHQSGPELVAQGFDPDTVQHVLRLVQNAEFKRHQAPPGLRITDRAFGTGWRMPIAKRQEGIS

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61623; Mature: 61623

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVALLQLNYIVGDLSGNAERIRSAVEAVAAAGAELAITSELALLGYPPRDLLLYPALVE
CEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCHHEECCCCHHHHHHHHHHH
RASQVLQHLASRLQASIPVVVGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYD
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCC
VFDEDRYFEPGSQPQPIEYRGRRLGFTICEDIWNDRDFWQHRRYHWDPVEQMARCGADLL
CCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCCCEE
INLSASPFSLGKQKLRVEMLGSLARKHALPILYVNQVGGNDDLIFDGASCAVDRRGRVVA
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCHHHCCCCCEEH
QAAAFQPDQLILDVDELTQSPNPDQSPPTAGSRWPTEPEAELYEALVLGTRDYVSKCGFR
HHHCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHH
EVLLGLSGGIDSAVTAVIAADALGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKL
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEHH
PIQSIMESYHQLLAEPFAGLPPDITEENLQSRIRGTLLMALSNKFGRLLLTTGNKSELAV
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
GYCTIYGDMSGGLAVISDVPKTWVYRLARWINRQQERIPLATLTKPPSAELRPGQQDSDS
EEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCHHHHCCCEEEECCCCCCCCCCCCCCCCC
LPPYDLLDRILQRHIEQHQSGPELVAQGFDPDTVQHVLRLVQNAEFKRHQAPPGLRITDR
CCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEECC
AFGTGWRMPIAKRQEGIS
CCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKVALLQLNYIVGDLSGNAERIRSAVEAVAAAGAELAITSELALLGYPPRDLLLYPALVE
CEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCHHEECCCCHHHHHHHHHHH
RASQVLQHLASRLQASIPVVVGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYD
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCC
VFDEDRYFEPGSQPQPIEYRGRRLGFTICEDIWNDRDFWQHRRYHWDPVEQMARCGADLL
CCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHCCCHHHHHHCCCCCCHHHHHHHCCCCEE
INLSASPFSLGKQKLRVEMLGSLARKHALPILYVNQVGGNDDLIFDGASCAVDRRGRVVA
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCHHHCCCCCEEH
QAAAFQPDQLILDVDELTQSPNPDQSPPTAGSRWPTEPEAELYEALVLGTRDYVSKCGFR
HHHCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHH
EVLLGLSGGIDSAVTAVIAADALGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKL
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEHH
PIQSIMESYHQLLAEPFAGLPPDITEENLQSRIRGTLLMALSNKFGRLLLTTGNKSELAV
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEE
GYCTIYGDMSGGLAVISDVPKTWVYRLARWINRQQERIPLATLTKPPSAELRPGQQDSDS
EEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCHHHHCCCEEEECCCCCCCCCCCCCCCCC
LPPYDLLDRILQRHIEQHQSGPELVAQGFDPDTVQHVLRLVQNAEFKRHQAPPGLRITDR
CCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEECC
AFGTGWRMPIAKRQEGIS
CCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]