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The map label for this gene is phoA [H]

Identifier: 86608674

GI number: 86608674

Start: 1242592

End: 1243941

Strand: Direct

Name: phoA [H]

Synonym: CYB_1198

Alternate gene names: 86608674

Gene position: 1242592-1243941 (Clockwise)

Preceding gene: 86608673

Following gene: 86608675

Centisome position: 40.79

GC content: 58.81

Gene sequence:

>1350_bases
GTGCTAGCTACGCGTAGCTATTCTCTGAGGGCAGCGTTGGCTGCGACTGTATGCGTTTTGTCTTCTTCCGTCTTCTCCTC
TAGCCTGGCTCAAGCAGCGCCTCAGCCGACCGGAAACGGGGCCATCTTCTTCCACCCGGATGGCACTTCGGCTTCTCACT
GGGACATCACACGCATTCTCCACTACGGCCCCGATGGCTTTCTGAACTGGGATCGTCTTCCCGTGATTACCACCTATCGC
GGGCACCTTTTCGACCAGCTTGGGGGAACTTCCAACGCCGGAGCGGTGACCCATGCCACAGGAACTCGTGCCCACGCCAA
GAGCTTTGGGTTGGATCCGGACGGCAAAGAGTACTCCTCTGCCAACGGCACCCTGAACACGATCATGGAAGAGGCTGTGG
CGGCAGGCTTGGGTACGGCTCTGGTGCAAACGGGATCCATCATTGAGCCGGGGACGGCTGCCTTCGTTGCCAAAGCTGCC
AAGCGCAGCGACTATGAGGAGATTGCCCTGCAGGTGGTGGAGTCAGGGGTAGACATCATCCTCGGCGCTGGCGAGGAATG
GCTGCTGCCGGAAGGTGTCCAGGGCCGCTTTGGCAAAGGGAAGCGCAAGGATGGCCGCAACCTGATTGAGGAGGCCAAGA
AAAAGGGGTACGCCGTTGTCTACACCCGCGACGAGCTGCTCAAGCTGCCGGCAGATGTCTCCAAGGTTTTGGGGGTGTTC
AACGTCGAGGACACCTTCTACGACAAGCCTGAAGAAGAGTTGCGGAAAGAAAACTTGCCCAACTACATCGCCAGCGCCCC
CACCATTGCCGAGATGACCGAATTTGCCCTGGCGCGGATCTCCCGCAATCCTAAGGGCTTCTTCGCCGTCATCGAAGAAG
AGGGCACTGATAACATGTGCAACAACCTCAATGCCTCTGGCTGCCTGGAGGCCATGAAGCGGGCCGACGATGCCATCGGG
GTGATTCTCAACTTTATCGAGAAGAATCCCAAGACCTTCATGATTACCACCAGCGACAGCAACGCCGGCGGGATGCAGTT
GGTGGATATCGAATCCGCCGAGGAGCCGCTGCCCCCTACTGACCCCAAAGGCAGTGGTGCCCCCATTGACGGAGTTGACG
GCACGGGCACAAAGCCGTTTATTTCTGCTCCTGACAAGAATGGCAAGCGCTTTCCCTTTGCCGTGGCTTGGGCTACCGGA
GATGACGTCGGCAGTGGAGTCATCGCTCGCGCTGCGGGCCTGAACGCTCGAGAGCTAGTGCCGGTCACTGGAATCCTGAA
CACAGACATCTACCGCATTCTCTACTTCGTGCTCTTTGGGGAGCGCATCCCCAGCGAGCTGAAGCCCTAG

Upstream 100 bases:

>100_bases
CACTTTGGGCACGTCGCACACTGGTAAGTTGCTCTGCTCTTGCAAACTCGTCCGAAGCTGTGTTACTTTTCTTAACGTTC
AGCTTTGAACGAGCAAGAGC

Downstream 100 bases:

>100_bases
TGTCTGCTGTGGTCACCTGCCTTGGGCGGGTGTTTGCCCCACAAGCTCTGTCCGTCACGGGGGATCCATCCAGTGGATCC
CCTTTTTTTATGAGAACCAC

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MLATRSYSLRAALAATVCVLSSSVFSSSLAQAAPQPTGNGAIFFHPDGTSASHWDITRILHYGPDGFLNWDRLPVITTYR
GHLFDQLGGTSNAGAVTHATGTRAHAKSFGLDPDGKEYSSANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAKAA
KRSDYEEIALQVVESGVDIILGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMCNNLNASGCLEAMKRADDAIG
VILNFIEKNPKTFMITTSDSNAGGMQLVDIESAEEPLPPTDPKGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATG
DDVGSGVIARAAGLNARELVPVTGILNTDIYRILYFVLFGERIPSELKP

Sequences:

>Translated_449_residues
MLATRSYSLRAALAATVCVLSSSVFSSSLAQAAPQPTGNGAIFFHPDGTSASHWDITRILHYGPDGFLNWDRLPVITTYR
GHLFDQLGGTSNAGAVTHATGTRAHAKSFGLDPDGKEYSSANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAKAA
KRSDYEEIALQVVESGVDIILGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMCNNLNASGCLEAMKRADDAIG
VILNFIEKNPKTFMITTSDSNAGGMQLVDIESAEEPLPPTDPKGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATG
DDVGSGVIARAAGLNARELVPVTGILNTDIYRILYFVLFGERIPSELKP
>Mature_449_residues
MLATRSYSLRAALAATVCVLSSSVFSSSLAQAAPQPTGNGAIFFHPDGTSASHWDITRILHYGPDGFLNWDRLPVITTYR
GHLFDQLGGTSNAGAVTHATGTRAHAKSFGLDPDGKEYSSANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAKAA
KRSDYEEIALQVVESGVDIILGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMCNNLNASGCLEAMKRADDAIG
VILNFIEKNPKTFMITTSDSNAGGMQLVDIESAEEPLPPTDPKGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATG
DDVGSGVIARAAGLNARELVPVTGILNTDIYRILYFVLFGERIPSELKP

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=324, Percent_Identity=28.0864197530864, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI157266292, Length=320, Percent_Identity=27.5, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI94721246, Length=320, Percent_Identity=27.1875, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI116734717, Length=317, Percent_Identity=25.8675078864353, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI294660770, Length=316, Percent_Identity=25.6329113924051, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI294660772, Length=258, Percent_Identity=26.3565891472868, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI48994877, Length=348, Percent_Identity=26.1494252873563, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320689, Length=367, Percent_Identity=25.8855585831063, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585213, Length=338, Percent_Identity=26.0355029585799, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358067, Length=321, Percent_Identity=25.5451713395639, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI85725200, Length=328, Percent_Identity=24.6951219512195, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI85815833, Length=328, Percent_Identity=24.6951219512195, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 47907; Mature: 47907

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATRSYSLRAALAATVCVLSSSVFSSSLAQAAPQPTGNGAIFFHPDGTSASHWDITRIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHH
HYGPDGFLNWDRLPVITTYRGHLFDQLGGTSNAGAVTHATGTRAHAKSFGLDPDGKEYSS
HCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCC
ANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAKAAKRSDYEEIALQVVESGVDII
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
LGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHEE
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMC
CCCHHHCCCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
NNLNASGCLEAMKRADDAIGVILNFIEKNPKTFMITTSDSNAGGMQLVDIESAEEPLPPT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCC
DPKGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATGDDVGSGVIARAAGLNARELV
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCHHHEE
PVTGILNTDIYRILYFVLFGERIPSELKP
EECCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLATRSYSLRAALAATVCVLSSSVFSSSLAQAAPQPTGNGAIFFHPDGTSASHWDITRIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHH
HYGPDGFLNWDRLPVITTYRGHLFDQLGGTSNAGAVTHATGTRAHAKSFGLDPDGKEYSS
HCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCEEEECCCCHHHHHHCCCCCCCCCCCC
ANGTLNTIMEEAVAAGLGTALVQTGSIIEPGTAAFVAKAAKRSDYEEIALQVVESGVDII
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEE
LGAGEEWLLPEGVQGRFGKGKRKDGRNLIEEAKKKGYAVVYTRDELLKLPADVSKVLGVF
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHEE
NVEDTFYDKPEEELRKENLPNYIASAPTIAEMTEFALARISRNPKGFFAVIEEEGTDNMC
CCCHHHCCCCHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
NNLNASGCLEAMKRADDAIGVILNFIEKNPKTFMITTSDSNAGGMQLVDIESAEEPLPPT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCC
DPKGSGAPIDGVDGTGTKPFISAPDKNGKRFPFAVAWATGDDVGSGVIARAAGLNARELV
CCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCHHHEE
PVTGILNTDIYRILYFVLFGERIPSELKP
EECCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]