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The map label for this gene is rpoD2 [H]

Identifier: 86608620

GI number: 86608620

Start: 1178803

End: 1179762

Strand: Reverse

Name: rpoD2 [H]

Synonym: CYB_1142

Alternate gene names: 86608620

Gene position: 1179762-1178803 (Counterclockwise)

Preceding gene: 86608627

Following gene: 86608618

Centisome position: 38.72

GC content: 58.23

Gene sequence:

>960_bases
ATGTCAGAAAGTCTTGACTTGCAAGATGCGGTGGGGCGTTTCTTAAAGACGATTGGCCGCTACTCGTTGCTCACCGCTGA
AGAAGAGCTCCGCCTGGCTCACCAGGTGCAGCGGATGGTGACGCTCTTGGAGCAACGGCAGCCCCATGAGTCTGTGGAAG
GTTGGAGCCAGCGGCTGGGGATCCCTTTAGCAGAGCTGCAGCAGATCCTGCGGCAGGGGGAAGCAGCCAAGAAACGAATG
GTGGAGTGTAACCTCCGCTTGGTGGTGTCGATTGCCAAGCGCTATCGCAATCGAGGCTTGCCGTTTCTGGATCTGATTCA
GGAAGGCACCATCGGCCTCACCCGCGCCGTGGAAAAGTTCGATCCCACCCGTGGCTACAAGTTCTCCACCTATGCCACTT
GGTGGATCCGGCAAAGCGTGAACCGGGGCATCCAAAACAAGGGGCGGATGATCCGTCTGCCGGCCCACATGTACGAAAAG
GTCTCCCAGGTGAAGCGGCGCTACCGCGAGTTATCTGCCGAAAGGGGATCCCGTCCCCAAGCGGAAGAAGTTTTTGATTC
TCTGGATATTGGCGATAACTACCGGGAGCTGATCACCCGCCACATGGCAGAGATGCGGTCTTTGAATGTGGGGGTAGGGG
ATGAAGACGGCACCGAGCTGCAGCACTTCATCCCAGATGAAGACAACGACCCTGTTGAACACCTAACCCAGCTTGCCCGT
CGAGAGGAAGTCCGCGCGCTGGTGGGCCAACTGAGTGAACGAGAACGACAGGTGATCGTGGCTCGCTATGGGCTGGATGG
ATCGAGGGGCCTAACGCTGCGGGAAATTGGGGAGCACATCGGCCTTTCGCGAGAGCGAGTCCGACAAATTGAGCGGCGGG
CCTTGCGGAAATTGCGGCGGCTGCAACAGGAATACTCCACGCCCAAAGCTGCCAAACCCTGGGTTAACGGGAACCTGTAA

Upstream 100 bases:

>100_bases
TGTTTGCAGAAAGGTCAGCTTCGTGTGTAGGAAGAGGGCCCGATGCAGCAGGAGAAGTAGAGATTCTGACAGGACATGAA
TTTATGGTGTGGGGTTTGAT

Downstream 100 bases:

>100_bases
CCGTTTGCGTCAGCGGTGCGCAGCACTGTAGAAAGGTTTTTTCACCACTTCGGCAGGCTGGCGCTGCCCCCGAATTTCAA
TTTCCAGCCGGGATCCCACC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSESLDLQDAVGRFLKTIGRYSLLTAEEELRLAHQVQRMVTLLEQRQPHESVEGWSQRLGIPLAELQQILRQGEAAKKRM
VECNLRLVVSIAKRYRNRGLPFLDLIQEGTIGLTRAVEKFDPTRGYKFSTYATWWIRQSVNRGIQNKGRMIRLPAHMYEK
VSQVKRRYRELSAERGSRPQAEEVFDSLDIGDNYRELITRHMAEMRSLNVGVGDEDGTELQHFIPDEDNDPVEHLTQLAR
REEVRALVGQLSERERQVIVARYGLDGSRGLTLREIGEHIGLSRERVRQIERRALRKLRRLQQEYSTPKAAKPWVNGNL

Sequences:

>Translated_319_residues
MSESLDLQDAVGRFLKTIGRYSLLTAEEELRLAHQVQRMVTLLEQRQPHESVEGWSQRLGIPLAELQQILRQGEAAKKRM
VECNLRLVVSIAKRYRNRGLPFLDLIQEGTIGLTRAVEKFDPTRGYKFSTYATWWIRQSVNRGIQNKGRMIRLPAHMYEK
VSQVKRRYRELSAERGSRPQAEEVFDSLDIGDNYRELITRHMAEMRSLNVGVGDEDGTELQHFIPDEDNDPVEHLTQLAR
REEVRALVGQLSERERQVIVARYGLDGSRGLTLREIGEHIGLSRERVRQIERRALRKLRRLQQEYSTPKAAKPWVNGNL
>Mature_318_residues
SESLDLQDAVGRFLKTIGRYSLLTAEEELRLAHQVQRMVTLLEQRQPHESVEGWSQRLGIPLAELQQILRQGEAAKKRMV
ECNLRLVVSIAKRYRNRGLPFLDLIQEGTIGLTRAVEKFDPTRGYKFSTYATWWIRQSVNRGIQNKGRMIRLPAHMYEKV
SQVKRRYRELSAERGSRPQAEEVFDSLDIGDNYRELITRHMAEMRSLNVGVGDEDGTELQHFIPDEDNDPVEHLTQLARR
EEVRALVGQLSERERQVIVARYGLDGSRGLTLREIGEHIGLSRERVRQIERRALRKLRRLQQEYSTPKAAKPWVNGNL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is a component of the biological clock pathway that affects the circadian expression of a subset of ge

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=248, Percent_Identity=43.1451612903226, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789098, Length=298, Percent_Identity=39.5973154362416, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789871, Length=237, Percent_Identity=28.2700421940928, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37048; Mature: 36917

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESLDLQDAVGRFLKTIGRYSLLTAEEELRLAHQVQRMVTLLEQRQPHESVEGWSQRLG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
IPLAELQQILRQGEAAKKRMVECNLRLVVSIAKRYRNRGLPFLDLIQEGTIGLTRAVEKF
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHC
DPTRGYKFSTYATWWIRQSVNRGIQNKGRMIRLPAHMYEKVSQVKRRYRELSAERGSRPQ
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
AEEVFDSLDIGDNYRELITRHMAEMRSLNVGVGDEDGTELQHFIPDEDNDPVEHLTQLAR
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHH
REEVRALVGQLSERERQVIVARYGLDGSRGLTLREIGEHIGLSRERVRQIERRALRKLRR
HHHHHHHHHHHHHHHHHEEHHEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LQQEYSTPKAAKPWVNGNL
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SESLDLQDAVGRFLKTIGRYSLLTAEEELRLAHQVQRMVTLLEQRQPHESVEGWSQRLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
IPLAELQQILRQGEAAKKRMVECNLRLVVSIAKRYRNRGLPFLDLIQEGTIGLTRAVEKF
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHC
DPTRGYKFSTYATWWIRQSVNRGIQNKGRMIRLPAHMYEKVSQVKRRYRELSAERGSRPQ
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
AEEVFDSLDIGDNYRELITRHMAEMRSLNVGVGDEDGTELQHFIPDEDNDPVEHLTQLAR
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHH
REEVRALVGQLSERERQVIVARYGLDGSRGLTLREIGEHIGLSRERVRQIERRALRKLRR
HHHHHHHHHHHHHHHHHEEHHEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LQQEYSTPKAAKPWVNGNL
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8665856; 1368828 [H]