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The map label for this gene is rpfG [H]

Identifier: 86608608

GI number: 86608608

Start: 1167880

End: 1168899

Strand: Reverse

Name: rpfG [H]

Synonym: CYB_1130

Alternate gene names: 86608608

Gene position: 1168899-1167880 (Counterclockwise)

Preceding gene: 86608609

Following gene: 86608607

Centisome position: 38.37

GC content: 56.37

Gene sequence:

>1020_bases
ATGCCCGTGACAGTTGGGATCCCTGTCACACCTTCTGCGGATGAGGCGGATGAGACAGAGATCGGTCATCTTTTGATCGT
CGATGATCAGCCCAACAGCCGCCTGCTGCTGGGGGATCTGCTGCAGAGCATTGGCTATCGGGTCAGCGAGGCCGAAAGCG
GCAATCAAGCCCTGCAAATGTTGAAAACCTTCCAGCCGGATCTGATCTTGCTGGATGTCATGATGCCGGGGTTAGATGGG
TTCGAGGTCTGTCGACGCATCAAAGCGAATGACGAGACACGCTTGATCCCGGTGGTATTGGTTACAGCGGCCAGCGATCA
CAAAAATCGCGTGCGCGGTATTGAAGCTGGGGCAGATGATTTCTTGAGCAAGCCCTTTGACGAGTCGGAATTGTTGGCGC
GGGTGCGTTCCCTCGTTCGCCAAAAGCACCTCAATGAAGACTTGGATCATGCTGCTCAGGTTCTGTTCGCCATTGCCCGC
AGTGTGGAAAGCCGGGATCCCACCACCGGGGATCACTGTGAGCGCCTGGCACAAATGGCCAAGAGCTTCGGCGAGTTTTT
GCATCTGCCCCGTCCCCTGATCAAGGTGCTGATGTGGGGAGGTTACCTGCACGACATCGGCAAAGTGGGCATACCCGATG
CCATTTTGGGTAAAACAGGCAAGCATACGCCGGAAGAGTGGGAGGTGATGAAATCCCATGTCTTGATCGGGGAGCAAATC
TGCCGCCCACTACGCACCATGAAAGAGGTGTTGCCCCTCATCCGTCACCACCACGAGCGTTGGGATGGCAGCGGTTATCC
CGACGGCCTCAAGGGGGAAGAGATCCCTCTGGTGGCCCGTATCTTCCAGATCATCGACATCTACGACGCCCTCACCTCGG
AGCGACCTTACAAGCGAGCGTTCACCGTTCAAGAGGCCATAAGCATCTTGCGGGAAGAAACAGACAGGGGTTGGCGGGAT
CCCCAGTTGGTGGAGTATTTCTTTGAGTTTCTGCGATCGCGGGCGCTGGATTTGGAGTAG

Upstream 100 bases:

>100_bases
TCTTTTTTCTCTGATGGGATTACCGTAGAGGTGTTGCTACTGGGATCCTGCAGTGACCAGCTTTTCCTGTGCCCAACTCG
CCAAACTCGAGATGCCCAAG

Downstream 100 bases:

>100_bases
GCTAGATAGGGCTTACTTGTAGTGTACCAGCAGGATAGAGTCTCTTGCCGGGTGCCGATCCGCTGCGTCGTTGTGTAGGG
TGTCGCCAGTTGTTTCCTCG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MPVTVGIPVTPSADEADETEIGHLLIVDDQPNSRLLLGDLLQSIGYRVSEAESGNQALQMLKTFQPDLILLDVMMPGLDG
FEVCRRIKANDETRLIPVVLVTAASDHKNRVRGIEAGADDFLSKPFDESELLARVRSLVRQKHLNEDLDHAAQVLFAIAR
SVESRDPTTGDHCERLAQMAKSFGEFLHLPRPLIKVLMWGGYLHDIGKVGIPDAILGKTGKHTPEEWEVMKSHVLIGEQI
CRPLRTMKEVLPLIRHHHERWDGSGYPDGLKGEEIPLVARIFQIIDIYDALTSERPYKRAFTVQEAISILREETDRGWRD
PQLVEYFFEFLRSRALDLE

Sequences:

>Translated_339_residues
MPVTVGIPVTPSADEADETEIGHLLIVDDQPNSRLLLGDLLQSIGYRVSEAESGNQALQMLKTFQPDLILLDVMMPGLDG
FEVCRRIKANDETRLIPVVLVTAASDHKNRVRGIEAGADDFLSKPFDESELLARVRSLVRQKHLNEDLDHAAQVLFAIAR
SVESRDPTTGDHCERLAQMAKSFGEFLHLPRPLIKVLMWGGYLHDIGKVGIPDAILGKTGKHTPEEWEVMKSHVLIGEQI
CRPLRTMKEVLPLIRHHHERWDGSGYPDGLKGEEIPLVARIFQIIDIYDALTSERPYKRAFTVQEAISILREETDRGWRD
PQLVEYFFEFLRSRALDLE
>Mature_338_residues
PVTVGIPVTPSADEADETEIGHLLIVDDQPNSRLLLGDLLQSIGYRVSEAESGNQALQMLKTFQPDLILLDVMMPGLDGF
EVCRRIKANDETRLIPVVLVTAASDHKNRVRGIEAGADDFLSKPFDESELLARVRSLVRQKHLNEDLDHAAQVLFAIARS
VESRDPTTGDHCERLAQMAKSFGEFLHLPRPLIKVLMWGGYLHDIGKVGIPDAILGKTGKHTPEEWEVMKSHVLIGEQIC
RPLRTMKEVLPLIRHHHERWDGSGYPDGLKGEEIPLVARIFQIIDIYDALTSERPYKRAFTVQEAISILREETDRGWRDP
QLVEYFFEFLRSRALDLE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=40.6779661016949, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788394, Length=119, Percent_Identity=40.3361344537815, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082012, Length=182, Percent_Identity=33.5164835164835, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=36.9747899159664, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786784, Length=182, Percent_Identity=32.967032967033, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788550, Length=171, Percent_Identity=30.4093567251462, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=38.655462184874, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1787229, Length=155, Percent_Identity=32.9032258064516, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=37.9310344827586, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693157, Length=116, Percent_Identity=39.6551724137931, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790863, Length=117, Percent_Identity=31.6239316239316, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI2367329, Length=126, Percent_Identity=38.0952380952381, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787375, Length=119, Percent_Identity=32.7731092436975, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790299, Length=179, Percent_Identity=31.8435754189944, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790437, Length=120, Percent_Identity=36.6666666666667, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789149, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788191, Length=110, Percent_Identity=33.6363636363636, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790552, Length=125, Percent_Identity=36, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788713, Length=127, Percent_Identity=33.8582677165354, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789402, Length=124, Percent_Identity=33.0645161290323, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38352; Mature: 38221

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTVGIPVTPSADEADETEIGHLLIVDDQPNSRLLLGDLLQSIGYRVSEAESGNQALQM
CCEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCHHCCCCHHHHHHH
LKTFQPDLILLDVMMPGLDGFEVCRRIKANDETRLIPVVLVTAASDHKNRVRGIEAGADD
HHHCCCCCEEHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCCHHH
FLSKPFDESELLARVRSLVRQKHLNEDLDHAAQVLFAIARSVESRDPTTGDHCERLAQMA
HHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KSFGEFLHLPRPLIKVLMWGGYLHDIGKVGIPDAILGKTGKHTPEEWEVMKSHVLIGEQI
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
CRPLRTMKEVLPLIRHHHERWDGSGYPDGLKGEEIPLVARIFQIIDIYDALTSERPYKRA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
FTVQEAISILREETDRGWRDPQLVEYFFEFLRSRALDLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PVTVGIPVTPSADEADETEIGHLLIVDDQPNSRLLLGDLLQSIGYRVSEAESGNQALQM
CEEECCCCCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHCCCHHCCCCHHHHHHH
LKTFQPDLILLDVMMPGLDGFEVCRRIKANDETRLIPVVLVTAASDHKNRVRGIEAGADD
HHHCCCCCEEHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCCHHH
FLSKPFDESELLARVRSLVRQKHLNEDLDHAAQVLFAIARSVESRDPTTGDHCERLAQMA
HHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KSFGEFLHLPRPLIKVLMWGGYLHDIGKVGIPDAILGKTGKHTPEEWEVMKSHVLIGEQI
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
CRPLRTMKEVLPLIRHHHERWDGSGYPDGLKGEEIPLVARIFQIIDIYDALTSERPYKRA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHH
FTVQEAISILREETDRGWRDPQLVEYFFEFLRSRALDLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]