Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 86608194

GI number: 86608194

Start: 724745

End: 726217

Strand: Reverse

Name: gatB [H]

Synonym: CYB_0708

Alternate gene names: 86608194

Gene position: 726217-724745 (Counterclockwise)

Preceding gene: 86608195

Following gene: 86608192

Centisome position: 23.84

GC content: 59.27

Gene sequence:

>1473_bases
ATGACTGTTGCTGCCCCTGCCAAAGTGCAATATGAAGCTGTGATCGGGTTGGAAGTTCACTGCCAGCTCAGCACCCGCTC
CAAGATCTTCTCCAGCAGCGCGACAGCCTTTGGCGCTCCCCCCAACACCCAGATTGACCCCATCTGCATGGGCCTGCCCG
GCACCCTGCCGGTGCTCAACGAAAAGGTGCTGGAGTACGCGGTCAAAGCGGGTTTGGCCTTGAATTGCACCATTGCCCCC
TACAGCAAGTTCGACCGCAAGCAGTACTTTTACCCCGACTTGCCCAAAAACTACCAAATCTCCCAATACGACCTGCCGAT
TGCTACCCACGGCTGGATTGAGATCCAGCTCAGCGACGGTCGTACCAAGCGCATCGGCATCACCCGTCTGCACATGGAAG
AAGATGCGGGCAAGCTGGTCCACGCTGGCAGCGACCGCCTATCGGGATCCAGCTACTCTTTGGTGGATTTCAACCGGGCG
GGGGTTCCCCTCATCGAGATCGTCAGCGAGCCGGACATCCGCAGCGGGGAAGAAGCGGCAGAGTACGTGCAAGAGCTGCG
CCGCATCCTGCGCTATGCCGGGCTGTGCGATGGCAACCTGCAGGAGGGATCCCTGCGCTGTGATGTGAACATCTCGGTGC
GTCCGCTGGGATCCCAGACCTTTGGCACGAAGGTGGAAATTAAAAACATGAACTCCTTCAACGCCATCCAACGGGCCATC
GAGTACGAGTTCAACCGCCAAGTCAAGGCTGTGGAGGCAGGCGAGCGGATCGTGCAAGAGACCCGCCTTTGGGAAGAAAA
CAGCCAGCGCACCATCAGCATGCGCAAGAAAGAGGGATCCAGCGACTATCGCTATTTCCCCGAGCCCGATCTGCCGCCCA
TTCGCGTTACCGAAGCGCAGAAAACCCGCTGGCAGGCAGAGCTGCCGGAATTGCCCGGTGTGAAGCGGCGACGTTACCAG
GAGGTGTACGGTCTCTCGGTGTACGATGCCCGCTACCTCAGCGATGAACGCAACACCGCCGAATACTTTGAAGCCGTGAT
CGCCGCCGGGGCCGATCCGAAGGCAGCCGCCAACTGGATGATGAGCGACATCGCCAGCTACCTCAACACCCACAAGCTGG
ACTATCCCGACATTGCCCTGAAGCCGGAAACCCTGGCCGAGCTGATTGGCCTCATCGAGCAGGGCACCATCTCCAGCAAA
ATCGCCAAGGAGATCCTGCCGGAACTGCTGGAAAAAGGGGGATCGGCCCGCGCCCTGGTGGAAGCTAAGGGCATGACCCA
AATCTCGGATAGCGCTCTGCTGGGGCAGATGATCGCCGAGGTGCTGGCAGAAAACCCCGAGCAACTGCAGCAGTATCGGG
GGGGCAAAACCAAGCTCTTCGGCTACTTTGTCGGGCAATTGATGAAAAAAACTCAGGGACGGGCAGATCCAAAGCTGGCC
AACGACCTGCTCAAGCAACACCTCGACGGCTAG

Upstream 100 bases:

>100_bases
CCTCGTCCTGGGTTCTGGGCTGCCGTCCCCAGAAACTGTCTGTGAGCTGGGATCCCCAAGGTCGCACTAGAATGGTTACA
CCGCCAGTTTTTCCCATCCC

Downstream 100 bases:

>100_bases
CTCCAAGCATCGGTGAGGGAATAGCCCAACTCCTGCATCATGCGCCGCAGCAGGGGCAAGCTCAGCCCGATAACATTGCC
GGGGCACCCCTCGATCCGCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTVAAPAKVQYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPICMGLPGTLPVLNEKVLEYAVKAGLALNCTIAP
YSKFDRKQYFYPDLPKNYQISQYDLPIATHGWIEIQLSDGRTKRIGITRLHMEEDAGKLVHAGSDRLSGSSYSLVDFNRA
GVPLIEIVSEPDIRSGEEAAEYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSQTFGTKVEIKNMNSFNAIQRAI
EYEFNRQVKAVEAGERIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQKTRWQAELPELPGVKRRRYQ
EVYGLSVYDARYLSDERNTAEYFEAVIAAGADPKAAANWMMSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSK
IAKEILPELLEKGGSARALVEAKGMTQISDSALLGQMIAEVLAENPEQLQQYRGGKTKLFGYFVGQLMKKTQGRADPKLA
NDLLKQHLDG

Sequences:

>Translated_490_residues
MTVAAPAKVQYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPICMGLPGTLPVLNEKVLEYAVKAGLALNCTIAP
YSKFDRKQYFYPDLPKNYQISQYDLPIATHGWIEIQLSDGRTKRIGITRLHMEEDAGKLVHAGSDRLSGSSYSLVDFNRA
GVPLIEIVSEPDIRSGEEAAEYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSQTFGTKVEIKNMNSFNAIQRAI
EYEFNRQVKAVEAGERIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQKTRWQAELPELPGVKRRRYQ
EVYGLSVYDARYLSDERNTAEYFEAVIAAGADPKAAANWMMSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSK
IAKEILPELLEKGGSARALVEAKGMTQISDSALLGQMIAEVLAENPEQLQQYRGGKTKLFGYFVGQLMKKTQGRADPKLA
NDLLKQHLDG
>Mature_489_residues
TVAAPAKVQYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPICMGLPGTLPVLNEKVLEYAVKAGLALNCTIAPY
SKFDRKQYFYPDLPKNYQISQYDLPIATHGWIEIQLSDGRTKRIGITRLHMEEDAGKLVHAGSDRLSGSSYSLVDFNRAG
VPLIEIVSEPDIRSGEEAAEYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSQTFGTKVEIKNMNSFNAIQRAIE
YEFNRQVKAVEAGERIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQKTRWQAELPELPGVKRRRYQE
VYGLSVYDARYLSDERNTAEYFEAVIAAGADPKAAANWMMSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSKI
AKEILPELLEKGGSARALVEAKGMTQISDSALLGQMIAEVLAENPEQLQQYRGGKTKLFGYFVGQLMKKTQGRADPKLAN
DLLKQHLDG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=38.6, Blast_Score=328, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI115534094, Length=382, Percent_Identity=35.8638743455497, Blast_Score=225, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=517, Percent_Identity=30.7543520309478, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=36.2525458248472, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54649; Mature: 54518

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAAPAKVQYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPICMGLPGTLPVLN
CCCCCCCCEEEEEEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHH
EKVLEYAVKAGLALNCTIAPYSKFDRKQYFYPDLPKNYQISQYDLPIATHGWIEIQLSDG
HHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCCCCCEEECCCEEECCEEEEEECCC
RTKRIGITRLHMEEDAGKLVHAGSDRLSGSSYSLVDFNRAGVPLIEIVSEPDIRSGEEAA
CEEEEEEEEEEECCCCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHH
EYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSQTFGTKVEIKNMNSFNAIQRAI
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHH
EYEFNRQVKAVEAGERIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQ
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCCCEEECCCH
KTRWQAELPELPGVKRRRYQEVYGLSVYDARYLSDERNTAEYFEAVIAAGADPKAAANWM
HHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHH
MSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSKIAKEILPELLEKGGSARALV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
EAKGMTQISDSALLGQMIAEVLAENPEQLQQYRGGKTKLFGYFVGQLMKKTQGRADPKLA
EECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHH
NDLLKQHLDG
HHHHHHHCCC
>Mature Secondary Structure 
TVAAPAKVQYEAVIGLEVHCQLSTRSKIFSSSATAFGAPPNTQIDPICMGLPGTLPVLN
CCCCCCCEEEEEEEEEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCHHHH
EKVLEYAVKAGLALNCTIAPYSKFDRKQYFYPDLPKNYQISQYDLPIATHGWIEIQLSDG
HHHHHHHHHCCCEEEEEECCCHHHCCCCCCCCCCCCCCCCEEECCCEEECCEEEEEECCC
RTKRIGITRLHMEEDAGKLVHAGSDRLSGSSYSLVDFNRAGVPLIEIVSEPDIRSGEEAA
CEEEEEEEEEEECCCCCCEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHH
EYVQELRRILRYAGLCDGNLQEGSLRCDVNISVRPLGSQTFGTKVEIKNMNSFNAIQRAI
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEECCCHHHHHHHHH
EYEFNRQVKAVEAGERIVQETRLWEENSQRTISMRKKEGSSDYRYFPEPDLPPIRVTEAQ
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCCCEEECCCH
KTRWQAELPELPGVKRRRYQEVYGLSVYDARYLSDERNTAEYFEAVIAAGADPKAAANWM
HHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHH
MSDIASYLNTHKLDYPDIALKPETLAELIGLIEQGTISSKIAKEILPELLEKGGSARALV
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEE
EAKGMTQISDSALLGQMIAEVLAENPEQLQQYRGGKTKLFGYFVGQLMKKTQGRADPKLA
EECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHH
NDLLKQHLDG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA