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The map label for this gene is ruvC

Identifier: 86607987

GI number: 86607987

Start: 506211

End: 506717

Strand: Direct

Name: ruvC

Synonym: CYB_0493

Alternate gene names: 86607987

Gene position: 506211-506717 (Clockwise)

Preceding gene: 86607986

Following gene: 86607988

Centisome position: 16.62

GC content: 58.78

Gene sequence:

>507_bases
GTGTTCAGCGTAAATGTATCAGACACCCCACCTGAGCAGCGCATCCTCGGCCTAGATCCAGGTCTGGGAACCATGGGTTT
TGGTTGCATTCTGGCGGGATCTGCGGGGTTGAAACCTGTGGATTATGGGGTGATCAGCACCCCCAGCCAACACTCGATGC
CGGAGCGGCTGCAAACGCTGCACGAGGATTTGAGCCAGTTGCTGCGCCAACTGCGCCCGCATCAGGTGGGGCTGGAAAAA
CTTTTTTTCTACAAAATGGGCAATACCATTGCCATCGCCCAAGCCCGTGGCGTGATCCTGCTCACCTTGGCCCAGCATCA
TCTGTTGCCGGTGGAGTTTACGCCCGCCCAAGTCAAGCAAAGTTTGACAGGCTACGGACGCGCCGACAAGCAGGCTGTAC
AAGCTGCCGTGGCCCGGGAACTCAACCTGCCCAGCTTGCCCCGCCCGGATGATGCCGCCGATGCCTTGGCGATTGCCATC
ACAGTCTGGCACCACCGCTTCTCTTGA

Upstream 100 bases:

>100_bases
CTTTGCGGGATACCGCCATTCTGTTTGGACTCCGTCCCGCTAACGCGAATGGAAACAAACAGAATGCTATCGCTGCATAG
TTGAACAGTGATGTTCAGCA

Downstream 100 bases:

>100_bases
GGGATCCCCTCCCTGTCAATCCCCTACCTTTAGCAGAGATAAAGATTGGCCAATAAAGATGGGCTAGACTCAGTACAGCG
AGAAGCACAAGCCCTATGCC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MFSVNVSDTPPEQRILGLDPGLGTMGFGCILAGSAGLKPVDYGVISTPSQHSMPERLQTLHEDLSQLLRQLRPHQVGLEK
LFFYKMGNTIAIAQARGVILLTLAQHHLLPVEFTPAQVKQSLTGYGRADKQAVQAAVARELNLPSLPRPDDAADALAIAI
TVWHHRFS

Sequences:

>Translated_168_residues
MFSVNVSDTPPEQRILGLDPGLGTMGFGCILAGSAGLKPVDYGVISTPSQHSMPERLQTLHEDLSQLLRQLRPHQVGLEK
LFFYKMGNTIAIAQARGVILLTLAQHHLLPVEFTPAQVKQSLTGYGRADKQAVQAAVARELNLPSLPRPDDAADALAIAI
TVWHHRFS
>Mature_168_residues
MFSVNVSDTPPEQRILGLDPGLGTMGFGCILAGSAGLKPVDYGVISTPSQHSMPERLQTLHEDLSQLLRQLRPHQVGLEK
LFFYKMGNTIAIAQARGVILLTLAQHHLLPVEFTPAQVKQSLTGYGRADKQAVQAAVARELNLPSLPRPDDAADALAIAI
TVWHHRFS

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=135, Percent_Identity=31.8518518518519, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_SYNJB (Q2JP15)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_476749.1
- ProteinModelPortal:   Q2JP15
- SMR:   Q2JP15
- STRING:   Q2JP15
- GeneID:   3902030
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0493
- TIGR:   CYB_0493
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- PhylomeDB:   Q2JP15
- ProtClustDB:   PRK00039
- BioCyc:   SSP321332:CYB_0493-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18281; Mature: 18281

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVNVSDTPPEQRILGLDPGLGTMGFGCILAGSAGLKPVDYGVISTPSQHSMPERLQTL
CEECCCCCCCCHHEEEECCCCCCHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
HEDLSQLLRQLRPHQVGLEKLFFYKMGNTIAIAQARGVILLTLAQHHLLPVEFTPAQVKQ
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEHHHCCCCEEECHHHHHH
SLTGYGRADKQAVQAAVARELNLPSLPRPDDAADALAIAITVWHHRFS
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSVNVSDTPPEQRILGLDPGLGTMGFGCILAGSAGLKPVDYGVISTPSQHSMPERLQTL
CEECCCCCCCCHHEEEECCCCCCHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
HEDLSQLLRQLRPHQVGLEKLFFYKMGNTIAIAQARGVILLTLAQHHLLPVEFTPAQVKQ
HHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEHHHCCCCEEECHHHHHH
SLTGYGRADKQAVQAAVARELNLPSLPRPDDAADALAIAITVWHHRFS
HHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA