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The map label for this gene is pleD [H]

Identifier: 86607844

GI number: 86607844

Start: 365638

End: 366588

Strand: Direct

Name: pleD [H]

Synonym: CYB_0347

Alternate gene names: 86607844

Gene position: 365638-366588 (Clockwise)

Preceding gene: 86607835

Following gene: 86607847

Centisome position: 12.0

GC content: 59.41

Gene sequence:

>951_bases
GTGCTTACTGCTAACCCGCACAATTCGTTGGGCCTCTCCATGCCTCTCGACCCTCTCTCTCAGTTGCCAGAGGAGCCAAC
CACTCAGATCCCCTTGGAGTTTTACGAGCGCAGACCCCAGCCTGAGGCAACTGCGGCCTTTCTGACGGTGCTCAGCGGCG
CCAATGTGGGCATGACGTTTCCCTTGGCGAGGGGTGGAGTTTTGGGGCGCAGCCACCGCGCGGACATTCAGCTCTTGGAT
CACGCCGTCTCCCGGCAGCACTGTCGGCTGGAAAGAGAAAGTGAGGGTTATGTCCTCACCGACCTGGAGAGCCTCAACGG
CACATATGTTAACGGCGAGCGCGTCAGCCGCGTTCTCCTGAAGGATGGAGATCGCATTCAAGTGGGGGCCAGCACCCTCA
AGTTTGCCTACTACGACGCGGCAGAAGAAGCCTTCTTTTTGCAAATGGCCACTGCTGCCCTCTATGACCCGCTAACCGGT
CTGTATAACCGACGTTTTTTCCTGAAGCAACTGGAGCTGGAGATCCCCTTTGCCCTTCGCCATCGCATTCCCCTCCACGT
GCTCTATCTGGATTTGGATGGGTTTAAGCAAATCAACGACCGCTGGGGGCACTGGGCTGGGGATCAAACCTTAATCCAGG
TGGCGCGGCGATTGCAAGCTCACGTTCGCCAAGACGATCTGGTGGCCCGCTTGGGGGGCGATGAGTTTGCCCTGCTGGTG
CGCGGGATCCCGAACTCGCAGATCCTAGGGCTGGCAGAACGCTTGCGGCAGGCTGTCCAGACCCTGTTGGTGCAGGCGGG
GACGGAGACCATCTCCCTAAGCTGCAGTGTGGGCATCGCCTCTCTGCTGGAGCTGGGAGAGGGAACTGACCCACAAAGTC
TGCTGGCTGCTGCCGACAAGGCCCTCTACCAAGCCAAAGCTGAGGGGGGTAACCGAGAGACTTTGCTGTAG

Upstream 100 bases:

>100_bases
AACTCCAGAGTTCCGCAAGTTTACAGATCAGAACCGGCGAGACATCCCTCGCTCACCCAAACACGGCCTGGGGAAAACCG
ACATAATACGGATATAACGA

Downstream 100 bases:

>100_bases
TTCAGGGCCAACTGCTGGAGTTTAGGATCCCTCTACCAAGCTGGCATAGGCTTCGGCACTCATGGTGTCTTCCAAGTCTT
CGGGGTTGGCAATGCGCACC

Product: GGDEF/FHA domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLTANPHNSLGLSMPLDPLSQLPEEPTTQIPLEFYERRPQPEATAAFLTVLSGANVGMTFPLARGGVLGRSHRADIQLLD
HAVSRQHCRLERESEGYVLTDLESLNGTYVNGERVSRVLLKDGDRIQVGASTLKFAYYDAAEEAFFLQMATAALYDPLTG
LYNRRFFLKQLELEIPFALRHRIPLHVLYLDLDGFKQINDRWGHWAGDQTLIQVARRLQAHVRQDDLVARLGGDEFALLV
RGIPNSQILGLAERLRQAVQTLLVQAGTETISLSCSVGIASLLELGEGTDPQSLLAAADKALYQAKAEGGNRETLL

Sequences:

>Translated_316_residues
MLTANPHNSLGLSMPLDPLSQLPEEPTTQIPLEFYERRPQPEATAAFLTVLSGANVGMTFPLARGGVLGRSHRADIQLLD
HAVSRQHCRLERESEGYVLTDLESLNGTYVNGERVSRVLLKDGDRIQVGASTLKFAYYDAAEEAFFLQMATAALYDPLTG
LYNRRFFLKQLELEIPFALRHRIPLHVLYLDLDGFKQINDRWGHWAGDQTLIQVARRLQAHVRQDDLVARLGGDEFALLV
RGIPNSQILGLAERLRQAVQTLLVQAGTETISLSCSVGIASLLELGEGTDPQSLLAAADKALYQAKAEGGNRETLL
>Mature_316_residues
MLTANPHNSLGLSMPLDPLSQLPEEPTTQIPLEFYERRPQPEATAAFLTVLSGANVGMTFPLARGGVLGRSHRADIQLLD
HAVSRQHCRLERESEGYVLTDLESLNGTYVNGERVSRVLLKDGDRIQVGASTLKFAYYDAAEEAFFLQMATAALYDPLTG
LYNRRFFLKQLELEIPFALRHRIPLHVLYLDLDGFKQINDRWGHWAGDQTLIQVARRLQAHVRQDDLVARLGGDEFALLV
RGIPNSQILGLAERLRQAVQTLLVQAGTETISLSCSVGIASLLELGEGTDPQSLLAAADKALYQAKAEGGNRETLL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=43.0379746835443, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1787262, Length=157, Percent_Identity=35.6687898089172, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=37.6543209876543, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=32.7956989247312, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=36.6459627329193, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1787802, Length=188, Percent_Identity=34.0425531914894, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=38.0952380952381, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=38.9221556886228, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787541, Length=155, Percent_Identity=37.4193548387097, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788956, Length=160, Percent_Identity=34.375, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787056, Length=174, Percent_Identity=32.183908045977, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=32.3529411764706, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=30.625, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787816, Length=191, Percent_Identity=28.2722513089005, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34940; Mature: 34940

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50006 FHA_DOMAIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTANPHNSLGLSMPLDPLSQLPEEPTTQIPLEFYERRPQPEATAAFLTVLSGANVGMTF
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEC
PLARGGVLGRSHRADIQLLDHAVSRQHCRLERESEGYVLTDLESLNGTYVNGERVSRVLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCEECHHHHHHHHH
KDGDRIQVGASTLKFAYYDAAEEAFFLQMATAALYDPLTGLYNRRFFLKQLELEIPFALR
CCCCEEEECHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HRIPLHVLYLDLDGFKQINDRWGHWAGDQTLIQVARRLQAHVRQDDLVARLGGDEFALLV
CCCCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEE
RGIPNSQILGLAERLRQAVQTLLVQAGTETISLSCSVGIASLLELGEGTDPQSLLAAADK
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHH
ALYQAKAEGGNRETLL
HHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLTANPHNSLGLSMPLDPLSQLPEEPTTQIPLEFYERRPQPEATAAFLTVLSGANVGMTF
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEC
PLARGGVLGRSHRADIQLLDHAVSRQHCRLERESEGYVLTDLESLNGTYVNGERVSRVLL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCEECHHHHHHHHH
KDGDRIQVGASTLKFAYYDAAEEAFFLQMATAALYDPLTGLYNRRFFLKQLELEIPFALR
CCCCEEEECHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HRIPLHVLYLDLDGFKQINDRWGHWAGDQTLIQVARRLQAHVRQDDLVARLGGDEFALLV
CCCCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEE
RGIPNSQILGLAERLRQAVQTLLVQAGTETISLSCSVGIASLLELGEGTDPQSLLAAADK
ECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHH
ALYQAKAEGGNRETLL
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA