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The map label for this gene is 86607762

Identifier: 86607762

GI number: 86607762

Start: 278269

End: 279717

Strand: Direct

Name: 86607762

Synonym: CYB_0263

Alternate gene names: NA

Gene position: 278269-279717 (Clockwise)

Preceding gene: 86607758

Following gene: 86607764

Centisome position: 9.13

GC content: 60.87

Gene sequence:

>1449_bases
ATGGTCAGCGTCGCTTCCCTTCCCTACAGCACCACCGACTGGCAATTGGGCTATCGCTCCCTATCCCAGGAGTACGACTA
TTGGATTACGGAGATCGAAGGACGGATCCCGGCAGGGTTGCGGGGAACCCTGTTTCGCAACGGCCCGGGCAAGCTGGAGG
CGGGATCCCAGCGCTACGGGCACCCGTTCGATGGCGATGGCATGGTCTGCGCCATCACCTTTGTGGAGGGGAAGGTTCAT
TTTCGCAACCGGTTTGTGCGCACCCGCGAGTTTGAAGCAGAGGAAAAAGCCGGTCGGGTGCTTTACCGAGGGGTGTTTGG
CACCCAAAAGCCGGGCGGCTGGTGGAGCAACTTTCTCGACTTGCGGTTTAAAAATCCCGCCAACACCAACGTCATCTACC
AGGGAGATAAGCTGCTGGCCCTGTGGGAGGCCAGTGCCCCTTACCGGCTGAACCCGGCCACCTTGGCCACCGAGGGGATC
GAAACGTTTGCAGGAGGGATCTCGGCCAGCCAGCCTTTCACTGCCCATCCCCGGCGGGATCCCCACACGGGAGATCTCAT
CGCCTTTGGGGTGCGGGCTGCCCAGAACTCCACGTTGCTGTTTTACCGTCTGGACTCGCGCGGGCGCTTGGTGGAAAAGC
GGGATTACCGCCTGCCCGGTTTTGCCTTTCTGCATGATTTTGTCTGGACACCCCGCTACCGCATCTTTTTCCAAAATCCC
TTGGCTTTCAATCCCCTGCCGTTTGTGTTGGGCTGGCAACCGGCGGGCACCTGTCTGACCTTGAAACCGGGGGAGCCGAC
CCGCATCTGGCTCTTTCCCCAGGGCGACGAGCCGCTGCAACTGGAAACGGAGCCGGGGTTTGTTTTCCATTTTGTCAACG
GCTACGGAGTCGGGGATCGGGTTGTGGTGGATGCCGTCCTCTATGAGAGCTACCCTGCCCTGGAGCCGGATGCCGATTAC
CTGCAGATTGACTTTGCCCAAATTCCAGCGGGCAAGCTGTGGCGGTTTTATCTGGATCCCAAGGGGGGCAGCGTGAAGCG
GGAACTGCTGCTGGATCGCTCGGTGGAGTTTCCGGCCATCCACCCGGCTCGAATGGGGGGATCCCACCGCTACATCTACA
TCGGGGCCACCCATGCCCCCGGCCCCAATGCTCCCCTACAGGCCATCCTCAAGCTGGATACCGCCACAGGCCGCACACAG
CAGTACAGCTTTGCCCCCCGCGGCTTTGTTGGAGAACCGGTGTTCATCCCCAATCCAGAAGACCAGGGCGAAGAGGCAGG
GTGGATCGTGACGGTGGTTTTTGATGCGGCCAGCCAGCGCTCTCAAGTGGTGATCCTAGAAGCAGCCGACCTGACAGCCG
GCCCGGTTGCGCGGATCCCATTGCGGCACCACATTCCCTATGGCCTACACGGCAGTTTTACTCCCCAGGTGTGGGTAACG
CCTGCCTAA

Upstream 100 bases:

>100_bases
ATCTTGGGGATCCCCACCCAGGAGACCATCCCGGCCCAGAAAAACTGGCGACTTCACAAAAGTTTAAGCAACAATAGAGG
GCGTTGCATGCAGATCTTCC

Downstream 100 bases:

>100_bases
GCGATGAGCTTCAAGATGGCTGAATGGATCCCATCGGCTCGGGAAAGATCATGCGCCCCAGCCTTTGCGGGAGCCCTGGG
GATCGGTCAGTCGGATCCCG

Product: lignostilbene-alpha,beta-dioxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MVSVASLPYSTTDWQLGYRSLSQEYDYWITEIEGRIPAGLRGTLFRNGPGKLEAGSQRYGHPFDGDGMVCAITFVEGKVH
FRNRFVRTREFEAEEKAGRVLYRGVFGTQKPGGWWSNFLDLRFKNPANTNVIYQGDKLLALWEASAPYRLNPATLATEGI
ETFAGGISASQPFTAHPRRDPHTGDLIAFGVRAAQNSTLLFYRLDSRGRLVEKRDYRLPGFAFLHDFVWTPRYRIFFQNP
LAFNPLPFVLGWQPAGTCLTLKPGEPTRIWLFPQGDEPLQLETEPGFVFHFVNGYGVGDRVVVDAVLYESYPALEPDADY
LQIDFAQIPAGKLWRFYLDPKGGSVKRELLLDRSVEFPAIHPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTATGRTQ
QYSFAPRGFVGEPVFIPNPEDQGEEAGWIVTVVFDAASQRSQVVILEAADLTAGPVARIPLRHHIPYGLHGSFTPQVWVT
PA

Sequences:

>Translated_482_residues
MVSVASLPYSTTDWQLGYRSLSQEYDYWITEIEGRIPAGLRGTLFRNGPGKLEAGSQRYGHPFDGDGMVCAITFVEGKVH
FRNRFVRTREFEAEEKAGRVLYRGVFGTQKPGGWWSNFLDLRFKNPANTNVIYQGDKLLALWEASAPYRLNPATLATEGI
ETFAGGISASQPFTAHPRRDPHTGDLIAFGVRAAQNSTLLFYRLDSRGRLVEKRDYRLPGFAFLHDFVWTPRYRIFFQNP
LAFNPLPFVLGWQPAGTCLTLKPGEPTRIWLFPQGDEPLQLETEPGFVFHFVNGYGVGDRVVVDAVLYESYPALEPDADY
LQIDFAQIPAGKLWRFYLDPKGGSVKRELLLDRSVEFPAIHPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTATGRTQ
QYSFAPRGFVGEPVFIPNPEDQGEEAGWIVTVVFDAASQRSQVVILEAADLTAGPVARIPLRHHIPYGLHGSFTPQVWVT
PA
>Mature_482_residues
MVSVASLPYSTTDWQLGYRSLSQEYDYWITEIEGRIPAGLRGTLFRNGPGKLEAGSQRYGHPFDGDGMVCAITFVEGKVH
FRNRFVRTREFEAEEKAGRVLYRGVFGTQKPGGWWSNFLDLRFKNPANTNVIYQGDKLLALWEASAPYRLNPATLATEGI
ETFAGGISASQPFTAHPRRDPHTGDLIAFGVRAAQNSTLLFYRLDSRGRLVEKRDYRLPGFAFLHDFVWTPRYRIFFQNP
LAFNPLPFVLGWQPAGTCLTLKPGEPTRIWLFPQGDEPLQLETEPGFVFHFVNGYGVGDRVVVDAVLYESYPALEPDADY
LQIDFAQIPAGKLWRFYLDPKGGSVKRELLLDRSVEFPAIHPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTATGRTQ
QYSFAPRGFVGEPVFIPNPEDQGEEAGWIVTVVFDAASQRSQVVILEAADLTAGPVARIPLRHHIPYGLHGSFTPQVWVT
PA

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI74027270, Length=517, Percent_Identity=24.1779497098646, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI4506591, Length=513, Percent_Identity=24.1715399610136, Blast_Score=103, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71998242, Length=509, Percent_Identity=24.1650294695481, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24646669, Length=304, Percent_Identity=26.6447368421053, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 53891; Mature: 53891

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSVASLPYSTTDWQLGYRSLSQEYDYWITEIEGRIPAGLRGTLFRNGPGKLEAGSQRYG
CCEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCC
HPFDGDGMVCAITFVEGKVHFRNRFVRTREFEAEEKAGRVLYRGVFGTQKPGGWWSNFLD
CCCCCCCEEEEEEEEECEEEEHHHEEEECCCCCHHHCCEEEEEECCCCCCCCCHHCCEEE
LRFKNPANTNVIYQGDKLLALWEASAPYRLNPATLATEGIETFAGGISASQPFTAHPRRD
EEECCCCCCCEEEECCEEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PHTGDLIAFGVRAAQNSTLLFYRLDSRGRLVEKRDYRLPGFAFLHDFVWTPRYRIFFQNP
CCCCCEEEEEEEECCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCCEEEEEECC
LAFNPLPFVLGWQPAGTCLTLKPGEPTRIWLFPQGDEPLQLETEPGFVFHFVNGYGVGDR
CCCCCCCEEEECCCCCCEEEECCCCCCEEEEEECCCCCEEEECCCCEEEEEECCCCCCCH
VVVDAVLYESYPALEPDADYLQIDFAQIPAGKLWRFYLDPKGGSVKRELLLDRSVEFPAI
HEEEEEHHCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCEEHHHHHCCCCCCCCC
HPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTATGRTQQYSFAPRGFVGEPVFIPNPE
CHHHCCCCEEEEEEEECCCCCCCCHHEEEEEEECCCCCCEEEECCCCCCCCCCEECCCCC
DQGEEAGWIVTVVFDAASQRSQVVILEAADLTAGPVARIPLRHHIPYGLHGSFTPQVWVT
CCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCEEEE
PA
CC
>Mature Secondary Structure
MVSVASLPYSTTDWQLGYRSLSQEYDYWITEIEGRIPAGLRGTLFRNGPGKLEAGSQRYG
CCEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCC
HPFDGDGMVCAITFVEGKVHFRNRFVRTREFEAEEKAGRVLYRGVFGTQKPGGWWSNFLD
CCCCCCCEEEEEEEEECEEEEHHHEEEECCCCCHHHCCEEEEEECCCCCCCCCHHCCEEE
LRFKNPANTNVIYQGDKLLALWEASAPYRLNPATLATEGIETFAGGISASQPFTAHPRRD
EEECCCCCCCEEEECCEEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PHTGDLIAFGVRAAQNSTLLFYRLDSRGRLVEKRDYRLPGFAFLHDFVWTPRYRIFFQNP
CCCCCEEEEEEEECCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHHHCCCCEEEEEECC
LAFNPLPFVLGWQPAGTCLTLKPGEPTRIWLFPQGDEPLQLETEPGFVFHFVNGYGVGDR
CCCCCCCEEEECCCCCCEEEECCCCCCEEEEEECCCCCEEEECCCCEEEEEECCCCCCCH
VVVDAVLYESYPALEPDADYLQIDFAQIPAGKLWRFYLDPKGGSVKRELLLDRSVEFPAI
HEEEEEHHCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCEEHHHHHCCCCCCCCC
HPARMGGSHRYIYIGATHAPGPNAPLQAILKLDTATGRTQQYSFAPRGFVGEPVFIPNPE
CHHHCCCCEEEEEEEECCCCCCCCHHEEEEEEECCCCCCEEEECCCCCCCCCCEECCCCC
DQGEEAGWIVTVVFDAASQRSQVVILEAADLTAGPVARIPLRHHIPYGLHGSFTPQVWVT
CCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCEEECCHHHCCCCCCCCCCCCCEEEE
PA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]