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The map label for this gene is xth [H]

Identifier: 86607730

GI number: 86607730

Start: 240593

End: 241378

Strand: Direct

Name: xth [H]

Synonym: CYB_0231

Alternate gene names: 86607730

Gene position: 240593-241378 (Clockwise)

Preceding gene: 86607729

Following gene: 86607731

Centisome position: 7.9

GC content: 56.23

Gene sequence:

>786_bases
ATGCAAGTGGCTACCTGGAATGTCAACTCCATTCGCACCCGGCTGCCTCAGGTTTTGGCTTGGCTGGACAGCCAGGGATC
CCTGGATGTGCTCTGCCTGCAGGAGACTAAGGTGGTGGATGAAGACTTTCCCCGGGCGGCCTTTCAGGAGCGGGGCTTTC
ATGTGGAGGTCTACGGGCAAAAGTCCTACAACGGTGTGGCTCTGATCAGCCGGGATCCCCCGGAAAAAGTAGAACGGGGG
TTTGGTGCGCTGCTGCCTTCGGCGGCAGATTTGGACGAGCAAAAGCGGCTGATGAGCGCCATCTACCGCGGGATCCGTAT
TCTCAACCTTTATGTGCCCAACGGTGCTGAGGTGGGATCGGAAAAATACGCCTATAAATTGCGCTGGCTGGCCACGCTGA
AGGATTACCTCGCGGTTGCCCTGAGCCAAGGGGATCCCATCTTGCTCTGCGGGGACTTTAACATTGCCCTGGAAGATCGG
GATATTTACGATCCCCAAAAAGCGGGAGAAATCATGGCTTCCGAACCGGAGCGACAAGCCCTGCAGGAGATCCTGGCTTT
AGGGTTTGAAGATGCGTTTCGCAGGTTCACCCCAGATCCCGGCCATTTTAGCTGGTGGGACTATCGCTCGGGGGGGTTTC
AAAGGAATCGCGGCTGGCGCATTGATCATCATTATCTGTCTCGTGAGCTACAGGAGAAAGCCACCGCTTGCTGGATCGAT
GTTGAGCCACGGCGGGCCGAAAAACCCAGCGATCATGCCCCTGTCATTGTTGCCCTGGATATTTAA

Upstream 100 bases:

>100_bases
CAAAAAAGCTGCCGGCTACTACACTGAGGGTGAGGTACTGGATGCGGAGCGGCTGGCCCAAATCCGAGGAAGAGCCTTGG
TGGAGTGAGCGGGATCCCTC

Downstream 100 bases:

>100_bases
GCGCCTTGTTATAGTCATTTCAAGCTAGGTTGAGACACTCCCCAAACGCTCCCTGCCACTGCCTTCACCCCTTCTCTCAG
AGGAAGAGGGGAGCCAGGCG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MQVATWNVNSIRTRLPQVLAWLDSQGSLDVLCLQETKVVDEDFPRAAFQERGFHVEVYGQKSYNGVALISRDPPEKVERG
FGALLPSAADLDEQKRLMSAIYRGIRILNLYVPNGAEVGSEKYAYKLRWLATLKDYLAVALSQGDPILLCGDFNIALEDR
DIYDPQKAGEIMASEPERQALQEILALGFEDAFRRFTPDPGHFSWWDYRSGGFQRNRGWRIDHHYLSRELQEKATACWID
VEPRRAEKPSDHAPVIVALDI

Sequences:

>Translated_261_residues
MQVATWNVNSIRTRLPQVLAWLDSQGSLDVLCLQETKVVDEDFPRAAFQERGFHVEVYGQKSYNGVALISRDPPEKVERG
FGALLPSAADLDEQKRLMSAIYRGIRILNLYVPNGAEVGSEKYAYKLRWLATLKDYLAVALSQGDPILLCGDFNIALEDR
DIYDPQKAGEIMASEPERQALQEILALGFEDAFRRFTPDPGHFSWWDYRSGGFQRNRGWRIDHHYLSRELQEKATACWID
VEPRRAEKPSDHAPVIVALDI
>Mature_261_residues
MQVATWNVNSIRTRLPQVLAWLDSQGSLDVLCLQETKVVDEDFPRAAFQERGFHVEVYGQKSYNGVALISRDPPEKVERG
FGALLPSAADLDEQKRLMSAIYRGIRILNLYVPNGAEVGSEKYAYKLRWLATLKDYLAVALSQGDPILLCGDFNIALEDR
DIYDPQKAGEIMASEPERQALQEILALGFEDAFRRFTPDPGHFSWWDYRSGGFQRNRGWRIDHHYLSRELQEKATACWID
VEPRRAEKPSDHAPVIVALDI

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=29.7397769516729, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=29.7397769516729, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=29.7397769516729, Blast_Score=117, Evalue=8e-27,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=35.0746268656716, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=28.7272727272727, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI221330655, Length=273, Percent_Identity=29.3040293040293, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17136678, Length=275, Percent_Identity=29.4545454545455, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29753; Mature: 29753

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVATWNVNSIRTRLPQVLAWLDSQGSLDVLCLQETKVVDEDFPRAAFQERGFHVEVYGQ
CEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCEEEEEEC
KSYNGVALISRDPPEKVERGFGALLPSAADLDEQKRLMSAIYRGIRILNLYVPNGAEVGS
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
EKYAYKLRWLATLKDYLAVALSQGDPILLCGDFNIALEDRDIYDPQKAGEIMASEPERQA
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCHHHHCCCCCCCHHHHH
LQEILALGFEDAFRRFTPDPGHFSWWDYRSGGFQRNRGWRIDHHYLSRELQEKATACWID
HHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEE
VEPRRAEKPSDHAPVIVALDI
CCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MQVATWNVNSIRTRLPQVLAWLDSQGSLDVLCLQETKVVDEDFPRAAFQERGFHVEVYGQ
CEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCEEEEEEC
KSYNGVALISRDPPEKVERGFGALLPSAADLDEQKRLMSAIYRGIRILNLYVPNGAEVGS
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCC
EKYAYKLRWLATLKDYLAVALSQGDPILLCGDFNIALEDRDIYDPQKAGEIMASEPERQA
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCHHHHCCCCCCCHHHHH
LQEILALGFEDAFRRFTPDPGHFSWWDYRSGGFQRNRGWRIDHHYLSRELQEKATACWID
HHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEE
VEPRRAEKPSDHAPVIVALDI
CCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]