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The map label for this gene is phrB-1 [H]

Identifier: 86607703

GI number: 86607703

Start: 212976

End: 214439

Strand: Direct

Name: phrB-1 [H]

Synonym: CYB_0202

Alternate gene names: 86607703

Gene position: 212976-214439 (Clockwise)

Preceding gene: 86607702

Following gene: 86607707

Centisome position: 6.99

GC content: 61.89

Gene sequence:

>1464_bases
ATGACTTCCCCCGTTTTGCTTTGGCATCGTCGCGATCTGCGGTTAGGGGACAACACAGCCCTGCATGAGGCAGCCCAGCG
CAGCTCCCAGGTGGTGCCTGTCTTCATTTTCGACCCGCAGATGCTGAGACGGTCGGACATGGCCCCAGCTAGGGTGGCCT
TTTTGCTTCAGGCTTTGCAAGAGCTCCAAGAGCGCTACGCCCAGATGGGGATCCCACTGATCTGGCGGCTGGGGGATCCC
TCGGTGGAGTTGCGGTGCTTGGCCATAGACCTGGGAGCCAGGGCCGTGTTTTGGAATGCCGATGGGGAGCCGTTCGCCCT
ACAGCAAGAGAGTCGAGTGCAGGCCAGTTTGGCCGCAGCCGGGATCCAGTCCTTCTCCTACCAAGACATGCTGCTGCACG
GGCCGGGGGAAGTGTTGACCCAGGCGGGGGAGCCCTATTCGGTCTTCACTCCCTTCTGGCGCAGGTGGGCGAGCTTGCCC
AAGCCGGATCCCTACCCGATTCCCAAAGGGCTACAACCGGTGTCCGGTCTGAAGGCGCAGCCTCTGCCCACTTTGGCAGA
TTTGGGCTTTGTCTGCGACCAGCGGATCCCAGCAGCGGGGGAAGCAGCAGCTCAGGCCCTTTTGGAAGACTTTTGTCAGG
GTTTGCGCATTTTGGACTACGAGCGAGCCCGCAACTTCCCGGCTGAGCCGGGCACTTCTCTACTGAGCCCCCACTTGTGC
TGGGGAACGATCGGCATTCGCCAGGTGTGGCAAGCCACGCGGGAGGTGGAAGCCGAGGTGCGCAGCCAAGAGGCCGAAAG
CAGCTTGCAAACTTGGCGGCAGGAGCTGTGCTGGCGAGAGTTTTACAAGCATGTCTTGGCCCACTGGCCCCACGTGGAAA
CCGGAGCCTATCGACGAGCCTTCGACGCCCTGGAGTGGGACAATCGGCAGGACTGGTTTCAGGCCTGGTGCACCGGTCAG
ACGGGCTACCCCATCGTGGATGCGGCCATGCGCCAACTGAACGAAACTGGCTGGATGCACAACCGCTGCCGCATGATTGT
GGCCAGCTTCCTGACCAAGGATCTGCTTATCGATTGGCGCTGGGGGGAACGCTACTTTATGCAAAAGCTGGTGGATGGGG
ATCTGGCGGCCAATAACGGCGGTTGGCAGTGGAGCGCCAGTGTGGGCACGGATCCCAAGCCCCTGCGCATTTTCAACCCG
GCCACGCAAGCAGCCCGCTATGACCCGGAAGGAGAGTACATCCGTCGCTATCTGCCGGAGCTGGCACGGCTGGATACGCC
GGCTCTGCTGTGGGTGGGAGATGACCCCAAGGGAGCCTGGGCTTTGCGGGAACGGCGAAGTTGTGGCTATCCGGATCCCA
TTGTGGATCACAAGGTGCAGCAGCAGGCGTTCAAACGGCGCTACCAGGCGGTGCAGCAGGCCTACTCTAGGTCTGTTCGA
GACGGTCTCCCCCCTCCTTCCTAG

Upstream 100 bases:

>100_bases
GAATGTAGCTTCTGGCCGCCCCGGCTCCCAGCCTTGGGGGGAGTGAGTTGAAAGGGACGGTGGCAACCAGCCAAGATGGG
ATCCCATTGCTTGGCAGCCT

Downstream 100 bases:

>100_bases
GTTGTGTTGATCTTTCATTTGAGCCAAAAGAGAGCACTGACGAGATAGAGAAAACTCAAGAAGTTCCTGGCCAATTTGTC
AAAACGAGAAAATAGATGCC

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTSPVLLWHRRDLRLGDNTALHEAAQRSSQVVPVFIFDPQMLRRSDMAPARVAFLLQALQELQERYAQMGIPLIWRLGDP
SVELRCLAIDLGARAVFWNADGEPFALQQESRVQASLAAAGIQSFSYQDMLLHGPGEVLTQAGEPYSVFTPFWRRWASLP
KPDPYPIPKGLQPVSGLKAQPLPTLADLGFVCDQRIPAAGEAAAQALLEDFCQGLRILDYERARNFPAEPGTSLLSPHLC
WGTIGIRQVWQATREVEAEVRSQEAESSLQTWRQELCWREFYKHVLAHWPHVETGAYRRAFDALEWDNRQDWFQAWCTGQ
TGYPIVDAAMRQLNETGWMHNRCRMIVASFLTKDLLIDWRWGERYFMQKLVDGDLAANNGGWQWSASVGTDPKPLRIFNP
ATQAARYDPEGEYIRRYLPELARLDTPALLWVGDDPKGAWALRERRSCGYPDPIVDHKVQQQAFKRRYQAVQQAYSRSVR
DGLPPPS

Sequences:

>Translated_487_residues
MTSPVLLWHRRDLRLGDNTALHEAAQRSSQVVPVFIFDPQMLRRSDMAPARVAFLLQALQELQERYAQMGIPLIWRLGDP
SVELRCLAIDLGARAVFWNADGEPFALQQESRVQASLAAAGIQSFSYQDMLLHGPGEVLTQAGEPYSVFTPFWRRWASLP
KPDPYPIPKGLQPVSGLKAQPLPTLADLGFVCDQRIPAAGEAAAQALLEDFCQGLRILDYERARNFPAEPGTSLLSPHLC
WGTIGIRQVWQATREVEAEVRSQEAESSLQTWRQELCWREFYKHVLAHWPHVETGAYRRAFDALEWDNRQDWFQAWCTGQ
TGYPIVDAAMRQLNETGWMHNRCRMIVASFLTKDLLIDWRWGERYFMQKLVDGDLAANNGGWQWSASVGTDPKPLRIFNP
ATQAARYDPEGEYIRRYLPELARLDTPALLWVGDDPKGAWALRERRSCGYPDPIVDHKVQQQAFKRRYQAVQQAYSRSVR
DGLPPPS
>Mature_486_residues
TSPVLLWHRRDLRLGDNTALHEAAQRSSQVVPVFIFDPQMLRRSDMAPARVAFLLQALQELQERYAQMGIPLIWRLGDPS
VELRCLAIDLGARAVFWNADGEPFALQQESRVQASLAAAGIQSFSYQDMLLHGPGEVLTQAGEPYSVFTPFWRRWASLPK
PDPYPIPKGLQPVSGLKAQPLPTLADLGFVCDQRIPAAGEAAAQALLEDFCQGLRILDYERARNFPAEPGTSLLSPHLCW
GTIGIRQVWQATREVEAEVRSQEAESSLQTWRQELCWREFYKHVLAHWPHVETGAYRRAFDALEWDNRQDWFQAWCTGQT
GYPIVDAAMRQLNETGWMHNRCRMIVASFLTKDLLIDWRWGERYFMQKLVDGDLAANNGGWQWSASVGTDPKPLRIFNPA
TQAARYDPEGEYIRRYLPELARLDTPALLWVGDDPKGAWALRERRSCGYPDPIVDHKVQQQAFKRRYQAVQQAYSRSVRD
GLPPPS

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=499, Percent_Identity=27.8557114228457, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI4758072, Length=509, Percent_Identity=27.1119842829077, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI188536103, Length=453, Percent_Identity=26.9315673289183, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1786926, Length=466, Percent_Identity=41.4163090128755, Blast_Score=290, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6324962, Length=509, Percent_Identity=31.041257367387, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17137248, Length=511, Percent_Identity=27.9843444227006, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24585455, Length=511, Percent_Identity=27.9843444227006, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24648152, Length=527, Percent_Identity=25.426944971537, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55541; Mature: 55409

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPVLLWHRRDLRLGDNTALHEAAQRSSQVVPVFIFDPQMLRRSDMAPARVAFLLQALQ
CCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEECHHHHHHCCCCHHHHHHHHHHHH
ELQERYAQMGIPLIWRLGDPSVELRCLAIDLGARAVFWNADGEPFALQQESRVQASLAAA
HHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCEEEEECCCCCCEEECHHHHHHHHHHHH
GIQSFSYQDMLLHGPGEVLTQAGEPYSVFTPFWRRWASLPKPDPYPIPKGLQPVSGLKAQ
HHHHCCCCHHEEECCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PLPTLADLGFVCDQRIPAAGEAAAQALLEDFCQGLRILDYERARNFPAEPGTSLLSPHLC
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHH
WGTIGIRQVWQATREVEAEVRSQEAESSLQTWRQELCWREFYKHVLAHWPHVETGAYRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FDALEWDNRQDWFQAWCTGQTGYPIVDAAMRQLNETGWMHNRCRMIVASFLTKDLLIDWR
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECC
WGERYFMQKLVDGDLAANNGGWQWSASVGTDPKPLRIFNPATQAARYDPEGEYIRRYLPE
CCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCHHHHHCCCCCHHHHHHHHHH
LARLDTPALLWVGDDPKGAWALRERRSCGYPDPIVDHKVQQQAFKRRYQAVQQAYSRSVR
HHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGLPPPS
CCCCCCC
>Mature Secondary Structure 
TSPVLLWHRRDLRLGDNTALHEAAQRSSQVVPVFIFDPQMLRRSDMAPARVAFLLQALQ
CCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEECHHHHHHCCCCHHHHHHHHHHHH
ELQERYAQMGIPLIWRLGDPSVELRCLAIDLGARAVFWNADGEPFALQQESRVQASLAAA
HHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCEEEEECCCCCCEEECHHHHHHHHHHHH
GIQSFSYQDMLLHGPGEVLTQAGEPYSVFTPFWRRWASLPKPDPYPIPKGLQPVSGLKAQ
HHHHCCCCHHEEECCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PLPTLADLGFVCDQRIPAAGEAAAQALLEDFCQGLRILDYERARNFPAEPGTSLLSPHLC
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCHH
WGTIGIRQVWQATREVEAEVRSQEAESSLQTWRQELCWREFYKHVLAHWPHVETGAYRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FDALEWDNRQDWFQAWCTGQTGYPIVDAAMRQLNETGWMHNRCRMIVASFLTKDLLIDWR
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECC
WGERYFMQKLVDGDLAANNGGWQWSASVGTDPKPLRIFNPATQAARYDPEGEYIRRYLPE
CCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCHHHHHCCCCCHHHHHHHHHH
LARLDTPALLWVGDDPKGAWALRERRSCGYPDPIVDHKVQQQAFKRRYQAVQQAYSRSVR
HHHCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGLPPPS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]