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The map label for this gene is phnJ [H]

Identifier: 86607667

GI number: 86607667

Start: 177458

End: 178324

Strand: Direct

Name: phnJ [H]

Synonym: CYB_0165

Alternate gene names: 86607667

Gene position: 177458-178324 (Clockwise)

Preceding gene: 86607666

Following gene: 86607668

Centisome position: 5.82

GC content: 61.71

Gene sequence:

>867_bases
TTGAAACCCAAGCATAGCTATGCCTACCTGGATCTGGAGGCCAAGCGAGAAATTCGGCGCAAGGTTCTCAAGGCCCTGGC
CATACCGGGCCATCAAGTTCCCTTTGCTAGCCGGGAAATGCCGCTGGCTCGCGGCTGGGGCACAGGGGGATTGCAGCTTA
CGCTCAGCCTGGTGGGGCCGCAGGACGTGGTGAAGGTCATTGACCAGGGGGCCGATGACTCTGTCAATGCCGCCAACTTG
CGCCGCTTCATTGCCCAGGTCAGCGGCGCTCGAACAACCACCGACACGCTGGAGGCCACCCTCATCCAAAGCCGCCACCG
CATCCCCGAGGAGCGGCTCCAAGAAGGTCAAATCCTAATCCTGCAAGTTCCCATCCCTGAGCCTCTGCGCTATGTGGAGC
CCTCTGACGCCAGGGCGCGGGAGCTACATGCTCTGGGCGACTATAGCCTGCTGTGGGTGGATCTTTTTGAGGAGCACCTA
CGCCACGGCCAGAGCCGCAAGGGAGCGGCCTACCCAGTTCTGGTCCATGGGCGCTATGGGATGGATCCCAGCCCTCTGCC
CCGCTGGGATCTGCCCAAGCTGCATCTGGCAGCCCATATCACCCTCTTTGGGGCCGGGCGGGAGAAGCGGCTCTATGCCG
TCCCTCCCTACACCCGGGTGGAGCCCTTGGCCTTTGTGGATCGGCCCTTCCAGCCGGAGCCCATTTTGGGATCCTGCCGT
CGCAGCGGTTGCCGCCACGTCTTCCTCAACGCCATTCCCCAAGAAGATGGCAGCTTGGCCTATGAAATCTCCGACCAGGG
CTATATGGAAAAGCTGCTGGCCGGGAAAACGAGCGGTCCTCTATTTGTGGAGAAAGAGCATGGCTGA

Upstream 100 bases:

>100_bases
GATCCCGTGGAGGCCTCCGGCTTTGCCCTCCACTACAAGCTACCCCACTACGTAACCTTTCTCAGCAACTGGATGAGCCA
CTTCCAAAAGGAGGCCTCCC

Downstream 100 bases:

>100_bases
GGAGACCCCCCTTCTGGAGCTGGTCAAGGTTTGCAAAGCCTACGGGGATCGCTGGGCGCTTAAGAACGTCAGCCTCAAGC
TCTATCCCCAAGAGGTGCTG

Product: phosphonate metabolism protein PhnJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKPKHSYAYLDLEAKREIRRKVLKALAIPGHQVPFASREMPLARGWGTGGLQLTLSLVGPQDVVKVIDQGADDSVNAANL
RRFIAQVSGARTTTDTLEATLIQSRHRIPEERLQEGQILILQVPIPEPLRYVEPSDARARELHALGDYSLLWVDLFEEHL
RHGQSRKGAAYPVLVHGRYGMDPSPLPRWDLPKLHLAAHITLFGAGREKRLYAVPPYTRVEPLAFVDRPFQPEPILGSCR
RSGCRHVFLNAIPQEDGSLAYEISDQGYMEKLLAGKTSGPLFVEKEHG

Sequences:

>Translated_288_residues
MKPKHSYAYLDLEAKREIRRKVLKALAIPGHQVPFASREMPLARGWGTGGLQLTLSLVGPQDVVKVIDQGADDSVNAANL
RRFIAQVSGARTTTDTLEATLIQSRHRIPEERLQEGQILILQVPIPEPLRYVEPSDARARELHALGDYSLLWVDLFEEHL
RHGQSRKGAAYPVLVHGRYGMDPSPLPRWDLPKLHLAAHITLFGAGREKRLYAVPPYTRVEPLAFVDRPFQPEPILGSCR
RSGCRHVFLNAIPQEDGSLAYEISDQGYMEKLLAGKTSGPLFVEKEHG
>Mature_288_residues
MKPKHSYAYLDLEAKREIRRKVLKALAIPGHQVPFASREMPLARGWGTGGLQLTLSLVGPQDVVKVIDQGADDSVNAANL
RRFIAQVSGARTTTDTLEATLIQSRHRIPEERLQEGQILILQVPIPEPLRYVEPSDARARELHALGDYSLLWVDLFEEHL
RHGQSRKGAAYPVLVHGRYGMDPSPLPRWDLPKLHLAAHITLFGAGREKRLYAVPPYTRVEPLAFVDRPFQPEPILGSCR
RSGCRHVFLNAIPQEDGSLAYEISDQGYMEKLLAGKTSGPLFVEKEHG

Specific function: Belongs to an operon involved in utilization of glyphosate and other phosphonates [H]

COG id: COG3627

COG function: function code P; Uncharacterized enzyme of phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phnJ family [H]

Homologues:

Organism=Escherichia coli, GI1790536, Length=274, Percent_Identity=51.4598540145985, Blast_Score=294, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010306 [H]

Pfam domain/function: PF06007 PhnJ [H]

EC number: NA

Molecular weight: Translated: 32239; Mature: 32239

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKHSYAYLDLEAKREIRRKVLKALAIPGHQVPFASREMPLARGWGTGGLQLTLSLVGP
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCH
QDVVKVIDQGADDSVNAANLRRFIAQVSGARTTTDTLEATLIQSRHRIPEERLQEGQILI
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCEEE
LQVPIPEPLRYVEPSDARARELHALGDYSLLWVDLFEEHLRHGQSRKGAAYPVLVHGRYG
EECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
MDPSPLPRWDLPKLHLAAHITLFGAGREKRLYAVPPYTRVEPLAFVDRPFQPEPILGSCR
CCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCHHHHCCCCCCCCHHHHHH
RSGCRHVFLNAIPQEDGSLAYEISDQGYMEKLLAGKTSGPLFVEKEHG
HCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure
MKPKHSYAYLDLEAKREIRRKVLKALAIPGHQVPFASREMPLARGWGTGGLQLTLSLVGP
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCH
QDVVKVIDQGADDSVNAANLRRFIAQVSGARTTTDTLEATLIQSRHRIPEERLQEGQILI
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCEEE
LQVPIPEPLRYVEPSDARARELHALGDYSLLWVDLFEEHLRHGQSRKGAAYPVLVHGRYG
EECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
MDPSPLPRWDLPKLHLAAHITLFGAGREKRLYAVPPYTRVEPLAFVDRPFQPEPILGSCR
CCCCCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCHHHHCCCCCCCCHHHHHH
RSGCRHVFLNAIPQEDGSLAYEISDQGYMEKLLAGKTSGPLFVEKEHG
HCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]