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The map label for this gene is speE

Identifier: 86607560

GI number: 86607560

Start: 70137

End: 71075

Strand: Direct

Name: speE

Synonym: CYB_0058

Alternate gene names: 86607560

Gene position: 70137-71075 (Clockwise)

Preceding gene: 86607559

Following gene: 86607561

Centisome position: 2.3

GC content: 60.17

Gene sequence:

>939_bases
ATGAGCAGCCCGAGAAGTTTCTTCTGGGTTCAGGAGTACTTTACCCCTTGGGACTACACCGCCCGTGCCGTCACGCGCAT
CTTGGCCTACCGAAAAACCCCTTTCCAGGAGATGCTGATCGCCGAGACGGGGGCCTTCGGCAAGGGTCTGATGCTGGATG
GGCATTGGCAGTCCACTACGGTGGATGAATTTCTGTACCACGAGGCTTTGGTTCATCCGGCTATGGTGCAGGTTGTCCAA
GCGGGCGGGATCCCCCGGCGGGTACTGGTGTTGGGGGGGGCAGAAGGGGCAACGCTGCGGGAGGTGCTGCGTTGGCGCTC
GGTGGAGCAGGTGGTGATGGTGGATATCGATGGGGAGGTGGTGGCCGCCTGTCGAGAGCACCTGCCGGAAATGCACCAGG
GATCCTTTGAAGATCCGCGGGTGGAGGTGGTGATTGCCGATGCCTTGGATTTTCTCCAAGAAACAGGCCCCATTTGGGAT
GTGATCCTTTCCGATCTCTCCGATCCCATTGAGTCCGGCCCTGCCTACCGCCTCTTCACCCAGGAGTTTTTCCGCCAGAT
CCGCTCCAAGTTGCAGCCAGATGGGGCTTTCACCATCCAAGCCGGGCCGACGGGGCCGGTTGAGCTTCATCAACACACCC
GCATTGTTCGCACCCTGAAAACGGTGTTTGCCGCCGTGCAGCCCTACGCCATCTATGCCCCCACCTACGGTGGCCCACTG
GGTTTTGCCCTGGCAGCTCAGGATCCCATCTCCCCCAGGCCAGAACCGGAGCAGATCGACCAGATTCTGAGCCAACAGTT
GGATCCCGAGCGAGGGGCACTGCAATTCATCGATGGCATCACCCTTCTGGGCCTCTACCAAGTCCCAGCTCACCTGCGCC
GTGCCATTGCCGCCGAGACTGTGGTTTATACCCTGGATAACCCGCCTCGCATTGAATGA

Upstream 100 bases:

>100_bases
TTTTTCCAAACTGTCATGGGGGCCATCTGTAGGCTGGGTGGGATCCGATTCACCCCAGAGGTCAGTCTATGTTGCTGGAA
AAAGCTGTAGCGAGGTGCTT

Downstream 100 bases:

>100_bases
GGAGCCATGCAGCGCTCAAAGGGATCCCAGGAGAGTTTGTCTTTGGTGCCATAACTTGGTAGATTCGTGAGCAGCCGAAT
TTTGCTTCCCTGATGAAAGT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSSPRSFFWVQEYFTPWDYTARAVTRILAYRKTPFQEMLIAETGAFGKGLMLDGHWQSTTVDEFLYHEALVHPAMVQVVQ
AGGIPRRVLVLGGAEGATLREVLRWRSVEQVVMVDIDGEVVAACREHLPEMHQGSFEDPRVEVVIADALDFLQETGPIWD
VILSDLSDPIESGPAYRLFTQEFFRQIRSKLQPDGAFTIQAGPTGPVELHQHTRIVRTLKTVFAAVQPYAIYAPTYGGPL
GFALAAQDPISPRPEPEQIDQILSQQLDPERGALQFIDGITLLGLYQVPAHLRRAIAAETVVYTLDNPPRIE

Sequences:

>Translated_312_residues
MSSPRSFFWVQEYFTPWDYTARAVTRILAYRKTPFQEMLIAETGAFGKGLMLDGHWQSTTVDEFLYHEALVHPAMVQVVQ
AGGIPRRVLVLGGAEGATLREVLRWRSVEQVVMVDIDGEVVAACREHLPEMHQGSFEDPRVEVVIADALDFLQETGPIWD
VILSDLSDPIESGPAYRLFTQEFFRQIRSKLQPDGAFTIQAGPTGPVELHQHTRIVRTLKTVFAAVQPYAIYAPTYGGPL
GFALAAQDPISPRPEPEQIDQILSQQLDPERGALQFIDGITLLGLYQVPAHLRRAIAAETVVYTLDNPPRIE
>Mature_311_residues
SSPRSFFWVQEYFTPWDYTARAVTRILAYRKTPFQEMLIAETGAFGKGLMLDGHWQSTTVDEFLYHEALVHPAMVQVVQA
GGIPRRVLVLGGAEGATLREVLRWRSVEQVVMVDIDGEVVAACREHLPEMHQGSFEDPRVEVVIADALDFLQETGPIWDV
ILSDLSDPIESGPAYRLFTQEFFRQIRSKLQPDGAFTIQAGPTGPVELHQHTRIVRTLKTVFAAVQPYAIYAPTYGGPLG
FALAAQDPISPRPEPEQIDQILSQQLDPERGALQFIDGITLLGLYQVPAHLRRAIAAETVVYTLDNPPRIE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=257, Percent_Identity=30.3501945525292, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1786312, Length=229, Percent_Identity=41.0480349344978, Blast_Score=168, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI212645616, Length=230, Percent_Identity=35.6521739130435, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25154106, Length=226, Percent_Identity=35.3982300884956, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323175, Length=262, Percent_Identity=33.206106870229, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6325326, Length=238, Percent_Identity=35.7142857142857, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24645443, Length=244, Percent_Identity=32.3770491803279, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21355941, Length=183, Percent_Identity=32.2404371584699, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_SYNJB (Q2JQ57)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_476322.1
- ProteinModelPortal:   Q2JQ57
- SMR:   Q2JQ57
- STRING:   Q2JQ57
- GeneID:   3899983
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0058
- TIGR:   CYB_0058
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   TRILAYR
- PhylomeDB:   Q2JQ57
- ProtClustDB:   CLSK947465
- BioCyc:   SSP321332:CYB_0058-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 34892; Mature: 34761

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 36-36 BINDING 95-95 BINDING 115-115 BINDING 165-165 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRSFFWVQEYFTPWDYTARAVTRILAYRKTPFQEMLIAETGAFGKGLMLDGHWQSTT
CCCCCCEEEHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEECCCCCCCH
VDEFLYHEALVHPAMVQVVQAGGIPRRVLVLGGAEGATLREVLRWRSVEQVVMVDIDGEV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHH
VAACREHLPEMHQGSFEDPRVEVVIADALDFLQETGPIWDVILSDLSDPIESGPAYRLFT
HHHHHHHCHHHHCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEHHH
QEFFRQIRSKLQPDGAFTIQAGPTGPVELHQHTRIVRTLKTVFAAVQPYAIYAPTYGGPL
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GFALAAQDPISPRPEPEQIDQILSQQLDPERGALQFIDGITLLGLYQVPAHLRRAIAAET
CEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVYTLDNPPRIE
EEEECCCCCCCC
>Mature Secondary Structure 
SSPRSFFWVQEYFTPWDYTARAVTRILAYRKTPFQEMLIAETGAFGKGLMLDGHWQSTT
CCCCCEEEHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEECCCCCCCH
VDEFLYHEALVHPAMVQVVQAGGIPRRVLVLGGAEGATLREVLRWRSVEQVVMVDIDGEV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHH
VAACREHLPEMHQGSFEDPRVEVVIADALDFLQETGPIWDVILSDLSDPIESGPAYRLFT
HHHHHHHCHHHHCCCCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEHHH
QEFFRQIRSKLQPDGAFTIQAGPTGPVELHQHTRIVRTLKTVFAAVQPYAIYAPTYGGPL
HHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
GFALAAQDPISPRPEPEQIDQILSQQLDPERGALQFIDGITLLGLYQVPAHLRRAIAAET
CEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVYTLDNPPRIE
EEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA