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The map label for this gene is folD

Identifier: 86606618

GI number: 86606618

Start: 1992867

End: 1993736

Strand: Reverse

Name: folD

Synonym: CYA_1974

Alternate gene names: 86606618

Gene position: 1993736-1992867 (Counterclockwise)

Preceding gene: 86606619

Following gene: 86606617

Centisome position: 67.98

GC content: 65.17

Gene sequence:

>870_bases
ATGTCTGCCAAGATCCTGGATGGCAAAGCCTTGGCTGGCCGGCTGCAAGCCGAGATGGCCGCTCAGGTGCAAGCTTGGCT
GCCGCGGGTGGGGCGTCCGCCGGGATTGGCAGTGCTGCGGGTGGGGGAGGATCCCGCCAGTGCTGCCTATGTGCGCGGCA
AAGAGCGGGCCTGTGAGCGAGTGGGCATTGCCTCCTTTGGCCGCCATTTTTCCGCCCAAGACTCCCCCGCCCATCTGCTC
GACGCCATCGCCCAACTCAACCAGGACGAGCGCGTCGACGGCATCCTCGTGCAACTGCCTCTGCCCCCCGGCTGGGATCC
CATCCCGCCCCTTCTGGCCATCGACCCCGCCAAAGACGTGGACGGTCTCCACCCCCTCAACCTGGGCCGGCTAGTGCGCG
GCGAGCCCGGCCTGCGCAGTTGTACGCCTCTAGGTGTGATGCGCCTGTTGCAAGCGGAAGGGATCCCCATTGCCGGACGA
AAAGCGGTGGTGGTCGGCCGCAGCCTCCTGGTGGGCAAGCCCCTGAGCCTGATGTTGTTGGCCGCCGACGCAACGGTTAC
CGTGGCCCACTCCCGTACACCCAACCTGGCCGAAGTAACCCGCAGTGCCGACATTGTGGTCATGGCTGTAGGGCGTCCTC
GCTTGCTCACGGCAGACATGGTGAAGCCGGGCGCCGTGGTTGTCGATGTAGGCATTAACCGTATCCAAGACCCTGACGGA
TCTGAGCAGTTGGTGGGAGATGTGGACTACGAAGCGGTCAAAGAGCGGGCCGCTGCCATCACGCCTGTACCAGGCGGCGT
GGGGCCGATGACGGTGACGATGCTGCTGGCCAACACCCTGGAAAGCTACAAGCTTCGCAGCCACCTGTAG

Upstream 100 bases:

>100_bases
GATCCCGCTCTGCCGTCGCCTCTTTGCTGCGCCGAGGCTTGACGGACAATGCTCGACCCGTAACAATCGTCAAGTTGTCA
AGATGTCTTCTGGGGCCCCC

Downstream 100 bases:

>100_bases
CCTGGAAGCTGTCCTCAAAATCCATGAACAACTGCCAATGGCCGGCCACCAGGAGGGAGCCTTGGAGACATCAGTGAGGC
TTTTTGTATAGCTTGTTACT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSAKILDGKALAGRLQAEMAAQVQAWLPRVGRPPGLAVLRVGEDPASAAYVRGKERACERVGIASFGRHFSAQDSPAHLL
DAIAQLNQDERVDGILVQLPLPPGWDPIPPLLAIDPAKDVDGLHPLNLGRLVRGEPGLRSCTPLGVMRLLQAEGIPIAGR
KAVVVGRSLLVGKPLSLMLLAADATVTVAHSRTPNLAEVTRSADIVVMAVGRPRLLTADMVKPGAVVVDVGINRIQDPDG
SEQLVGDVDYEAVKERAAAITPVPGGVGPMTVTMLLANTLESYKLRSHL

Sequences:

>Translated_289_residues
MSAKILDGKALAGRLQAEMAAQVQAWLPRVGRPPGLAVLRVGEDPASAAYVRGKERACERVGIASFGRHFSAQDSPAHLL
DAIAQLNQDERVDGILVQLPLPPGWDPIPPLLAIDPAKDVDGLHPLNLGRLVRGEPGLRSCTPLGVMRLLQAEGIPIAGR
KAVVVGRSLLVGKPLSLMLLAADATVTVAHSRTPNLAEVTRSADIVVMAVGRPRLLTADMVKPGAVVVDVGINRIQDPDG
SEQLVGDVDYEAVKERAAAITPVPGGVGPMTVTMLLANTLESYKLRSHL
>Mature_288_residues
SAKILDGKALAGRLQAEMAAQVQAWLPRVGRPPGLAVLRVGEDPASAAYVRGKERACERVGIASFGRHFSAQDSPAHLLD
AIAQLNQDERVDGILVQLPLPPGWDPIPPLLAIDPAKDVDGLHPLNLGRLVRGEPGLRSCTPLGVMRLLQAEGIPIAGRK
AVVVGRSLLVGKPLSLMLLAADATVTVAHSRTPNLAEVTRSADIVVMAVGRPRLLTADMVKPGAVVVDVGINRIQDPDGS
EQLVGDVDYEAVKERAAAITPVPGGVGPMTVTMLLANTLESYKLRSHL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=47.098976109215, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=45.2380952380952, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=43.7931034482759, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=27.1356783919598, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786741, Length=282, Percent_Identity=48.936170212766, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=43.4615384615385, Blast_Score=193, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6319558, Length=281, Percent_Identity=43.4163701067616, Blast_Score=228, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=293, Percent_Identity=39.5904436860068, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=317, Percent_Identity=25.5520504731861, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=48.2993197278912, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=48.2993197278912, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=44.7916666666667, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=44.7916666666667, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=44.5205479452055, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=44.5205479452055, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30469; Mature: 30338

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKILDGKALAGRLQAEMAAQVQAWLPRVGRPPGLAVLRVGEDPASAAYVRGKERACER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCHHHHHHH
VGIASFGRHFSAQDSPAHLLDAIAQLNQDERVDGILVQLPLPPGWDPIPPLLAIDPAKDV
HCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCC
DGLHPLNLGRLVRGEPGLRSCTPLGVMRLLQAEGIPIAGRKAVVVGRSLLVGKPLSLMLL
CCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHCCCCEEEEE
AADATVTVAHSRTPNLAEVTRSADIVVMAVGRPRLLTADMVKPGAVVVDVGINRIQDPDG
ECCCEEEEEECCCCCHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCC
SEQLVGDVDYEAVKERAAAITPVPGGVGPMTVTMLLANTLESYKLRSHL
CHHHCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAKILDGKALAGRLQAEMAAQVQAWLPRVGRPPGLAVLRVGEDPASAAYVRGKERACER
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHCCHHHHHHH
VGIASFGRHFSAQDSPAHLLDAIAQLNQDERVDGILVQLPLPPGWDPIPPLLAIDPAKDV
HCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCC
DGLHPLNLGRLVRGEPGLRSCTPLGVMRLLQAEGIPIAGRKAVVVGRSLLVGKPLSLMLL
CCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHCCCCEEEEE
AADATVTVAHSRTPNLAEVTRSADIVVMAVGRPRLLTADMVKPGAVVVDVGINRIQDPDG
ECCCEEEEEECCCCCHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEEECCCCCCCCCCC
SEQLVGDVDYEAVKERAAAITPVPGGVGPMTVTMLLANTLESYKLRSHL
CHHHCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA