| Definition | Synechococcus sp. JA-3-3Ab, complete genome. |
|---|---|
| Accession | NC_007775 |
| Length | 2,932,766 |
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The map label for this gene is nifS
Identifier: 86606477
GI number: 86606477
Start: 1831239
End: 1832429
Strand: Reverse
Name: nifS
Synonym: CYA_1826
Alternate gene names: 86606477
Gene position: 1832429-1831239 (Counterclockwise)
Preceding gene: 86606478
Following gene: 86606476
Centisome position: 62.48
GC content: 63.9
Gene sequence:
>1191_bases GTGATCTACCTGGACAACAACGCCACCACCGCCGTGGATCCCCTGGTTCTGGAGGCGATGCTGCCCTATTTGCAGACCTT CTACGGCAACCCCTCCTCGATGCACAGTTTTGGCGGCCAGGTGGGGCGTGCCCTTAACCAAGCCCGCGAACAGGTGGCAG CCCTTCTGGGAGCCGACCCGACGGAGATTGTCTTCACCAGTGGCGGCACCGAAGGCAACACCACGGCCATTTATGCAGCC TTGGCAGCCCAACCCCAGAAGCGCCACATCATCACCACGCAGGTAGAGCACGCCTCGGTGCTCAACGTCTGCAAGCACCT GGAAAAGCAAGGCTACCGCGTCACCTACCTCTCGGTGGATGAGCAAGGCTGCCTGGACTTGCTGGAGTTGGAAGCAGCCC TGACCGGCGATACTGCCCTGGTGAGCTGCATGGCCGCCAACAACGAGACGGGCGTGCTTTTCCCCATCGAGGCGGTGGGA TCCCTGGCTCGCAGCTATGGGGCTGTTGTCCATGTGGACGCAGTGCAGGCGGTGGGCAAGATCCCTCTCGATCTGCAGCG CCTGCCGGTGGATCTCCTCACCCTTTCCGGGCACAAGCTGCATGCCCCCAAGGGGATCGGCGCTCTCTATGTGCGGCGGG GCTTGCGCTTCCGTCCGTTGCTGCTGGGAGGGCATCAGGAGCGAGGCCGCCGCGCCGGGACGGAAAATGTGCCGGGCATC ATCGCCCTGGGCAAGGCAGCGGAGCTGGCCCAAGCCCACCTCGGGGATCCCCATGAGCGGCTGCTGCGGGATCGCTTGGA GCAGGGCTTGCTACAGCGGATCCCGGATGTCCAGGTTAACGGCCACCCCACTCAGCGGCTGCCCAACACCACCAACTTGG GCTTCAAGTTCGTGGAAGGGGAAGCGATCCTCTTCTGGCTGAACAAATACGGCATCTGCGCCTCCTCCGGCTCCGCCTGC ACTTCCGGATCCCTGGAACCCTCCCATGTGCTGCGGGCCATGCGCCTGCCCTACACCGTTTTGCACGGCTCAATCCGCTT CAGCCTCTCCCGCTTTACCCAGCCGGCAGAGGTGGAGCGGGTGCTGGAGGTTTTGCCGGGGATCATTGAGCATTTGCGGG CCATCTCCCCCTTCAGCCACGAGGCGGAAGACTGGCTGCAACAGCAGGAACGGGAACTGGCTCGCCGCTGA
Upstream 100 bases:
>100_bases CGCCCGCATTTGCCCAAAGCAGTGCCATTCCCACGCTCCGCAAGCCGTTTAACGTTGCCCAACCCCTACCTTGTATCGAT TCGGAGCCTGTATGAACCCC
Downstream 100 bases:
>100_bases CCCCCCTCAAACTCATCTTCCCTCGACCGTTAGCATCCTCAACTCAGGATCCAGCCATGTGGGACTACAGCGAAAAAGTT TTGGAACTCTTCTACCACCC
Product: cysteine desulfurase
Products: NA
Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS2 [H]
Number of amino acids: Translated: 396; Mature: 396
Protein sequence:
>396_residues MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADPTEIVFTSGGTEGNTTAIYAA LAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVDEQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVG SLARSYGAVVHVDAVQAVGKIPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGLRFRPLLLGGHQERGRRAGTENVPGI IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEGEAILFWLNKYGICASSGSAC TSGSLEPSHVLRAMRLPYTVLHGSIRFSLSRFTQPAEVERVLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR
Sequences:
>Translated_396_residues MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADPTEIVFTSGGTEGNTTAIYAA LAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVDEQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVG SLARSYGAVVHVDAVQAVGKIPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGLRFRPLLLGGHQERGRRAGTENVPGI IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEGEAILFWLNKYGICASSGSAC TSGSLEPSHVLRAMRLPYTVLHGSIRFSLSRFTQPAEVERVLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR >Mature_396_residues MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADPTEIVFTSGGTEGNTTAIYAA LAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVDEQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVG SLARSYGAVVHVDAVQAVGKIPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGLRFRPLLLGGHQERGRRAGTENVPGI IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEGEAILFWLNKYGICASSGSAC TSGSLEPSHVLRAMRLPYTVLHGSIRFSLSRFTQPAEVERVLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR
Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]
COG id: COG1104
COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]
Homologues:
Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=48.0719794344473, Blast_Score=358, Evalue=5e-99, Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=36.231884057971, Blast_Score=213, Evalue=2e-55, Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=48.3116883116883, Blast_Score=338, Evalue=3e-94, Organism=Escherichia coli, GI1787970, Length=389, Percent_Identity=28.0205655526992, Blast_Score=99, Evalue=3e-22, Organism=Escherichia coli, GI1789175, Length=370, Percent_Identity=30.5405405405405, Blast_Score=99, Evalue=5e-22, Organism=Caenorhabditis elegans, GI25143064, Length=396, Percent_Identity=45.2020202020202, Blast_Score=327, Evalue=8e-90, Organism=Caenorhabditis elegans, GI17533177, Length=323, Percent_Identity=35.6037151702786, Blast_Score=176, Evalue=2e-44, Organism=Saccharomyces cerevisiae, GI6319831, Length=395, Percent_Identity=44.8101265822785, Blast_Score=322, Evalue=7e-89, Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=48.0519480519481, Blast_Score=338, Evalue=4e-93,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000192 - InterPro: IPR020578 - InterPro: IPR017772 - InterPro: IPR016454 - InterPro: IPR015424 - InterPro: IPR015421 - InterPro: IPR015422 [H]
Pfam domain/function: PF00266 Aminotran_5 [H]
EC number: =2.8.1.7 [H]
Molecular weight: Translated: 43142; Mature: 43142
Theoretical pI: Translated: 6.92; Mature: 6.92
Prosite motif: PS00595 AA_TRANSFER_CLASS_5
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 1.3 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADP CEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC TEIVFTSGGTEGNTTAIYAALAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVD CEEEEECCCCCCCCCEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEC EQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVGSLARSYGAVVHVDAVQAVGK CCHHHHHHHHHHHHCCCHHHHEEEECCCCCCEEEEHHHHHHHHHHCCCEEEEHHHHHHCC IPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGLRFRPLLLGGHQERGRRAGTENVPGI CCCCHHHCCEEEEEECCCEEECCCCCHHHHHHCCCCCCEEEECCCHHHHCCCCCCCCCCE IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEG EEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCHHCCCCCCCCCEEEECC EAILFWLNKYGICASSGSACTSGSLEPSHVLRAMRLPYTVLHGSIRFSLSRFTQPAEVER CEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHH VLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC >Mature Secondary Structure MIYLDNNATTAVDPLVLEAMLPYLQTFYGNPSSMHSFGGQVGRALNQAREQVAALLGADP CEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC TEIVFTSGGTEGNTTAIYAALAAQPQKRHIITTQVEHASVLNVCKHLEKQGYRVTYLSVD CEEEEECCCCCCCCCEEEEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEC EQGCLDLLELEAALTGDTALVSCMAANNETGVLFPIEAVGSLARSYGAVVHVDAVQAVGK CCHHHHHHHHHHHHCCCHHHHEEEECCCCCCEEEEHHHHHHHHHHCCCEEEEHHHHHHCC IPLDLQRLPVDLLTLSGHKLHAPKGIGALYVRRGLRFRPLLLGGHQERGRRAGTENVPGI CCCCHHHCCEEEEEECCCEEECCCCCHHHHHHCCCCCCEEEECCCHHHHCCCCCCCCCCE IALGKAAELAQAHLGDPHERLLRDRLEQGLLQRIPDVQVNGHPTQRLPNTTNLGFKFVEG EEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCHHCCCCCCCCCEEEECC EAILFWLNKYGICASSGSACTSGSLEPSHVLRAMRLPYTVLHGSIRFSLSRFTQPAEVER CEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHH VLEVLPGIIEHLRAISPFSHEAEDWLQQQERELARR HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7568132 [H]