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The map label for this gene is nifU

Identifier: 86606476

GI number: 86606476

Start: 1830244

End: 1831182

Strand: Reverse

Name: nifU

Synonym: CYA_1825

Alternate gene names: 86606476

Gene position: 1831182-1830244 (Counterclockwise)

Preceding gene: 86606477

Following gene: 86606475

Centisome position: 62.44

GC content: 60.38

Gene sequence:

>939_bases
ATGTGGGACTACAGCGAAAAAGTTTTGGAACTCTTCTACCACCCCAAAAACCAAGGGGCGATGGATCCCAGCTCCGAGCC
TGGTGTTCGGGTGGTGGTGGGAGAAGTGGGCAGCATCACCTGTGGCGATGCCCTGCGGCTCTACTTAAAGGTGGACGAGG
CTAGCGAGCGCATCTTGGCTGCCAGCTTCCAAACCTTCGGCTGCACCAGCGCCATTGCCTCTTCCTCGGCCCTGACGGAG
CTAATCACCGGCCTCACTCTGGATGAAGCCCTGCGGATCGACAACCGCCAAATCGCCGATTACCTGGGCGGCTTGCCCCC
CGCCAAGATGCACTGCTCGGTGATGGGGCAAGAGGCTCTGGAGGCAGCCATCTACAGCTATCGCGGCCTTCCTCTGCCTG
ACCACACGGATGATGACAGCGCTTTGGTCTGCTCCTGCTTTGGCGTCAGCGAGCAAAAGATCCGCCGCCTGATCCGGGAA
AACCACCTCACCTCCGCTGAGGAAGTAACAGCCTACAGCAAAGCGGGCGGCGGTTGTGGCTCCTGCCTGGCCCAGATTGA
AGACCTGATCGCCGCCGTTCTGGCCGAGACTGACGCAACCCCTTCAGAAGATTCAAGAAATTCAAAAGGGAACTCCCAAA
ACCAGACAGAGCCCTCTCCCGCTGCTCCCTCTCCTGTCTCGGCAACAGCGACACCCCCTCCCCTCACCACCTTGCAAAAG
ATCGCCCGCATTCAGGCCGTCCTGGAGGAGGAAGTTCGCCCGCTGCTGTTGGCCGATGGCGGCGATGTGGAGCTGTACGA
CCTGGAAGGGGATCAGGTGTGGGTACGCCTCAAGGGATCCTGCACCACCTGCCCCAGCCAGCAAAACACCCTGCGGTTGC
TCATCGAAACCCGCCTGCAAGAACAGGTGTGGCCCACCTTAACCGTGCATGCCCTCTAG

Upstream 100 bases:

>100_bases
AAGACTGGCTGCAACAGCAGGAACGGGAACTGGCTCGCCGCTGACCCCCCTCAAACTCATCTTCCCTCGACCGTTAGCAT
CCTCAACTCAGGATCCAGCC

Downstream 100 bases:

>100_bases
TTGCCCATTCTCGATAAGCCTGAGAGCCCTATGATCTGCCCCACTGTTACTCCCCGTCGCTTCCCCCATCTCCTGCCTCG
ATTCGGGAGAGCCCCACCGC

Product: Fe-S cluster assembly protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MWDYSEKVLELFYHPKNQGAMDPSSEPGVRVVVGEVGSITCGDALRLYLKVDEASERILAASFQTFGCTSAIASSSALTE
LITGLTLDEALRIDNRQIADYLGGLPPAKMHCSVMGQEALEAAIYSYRGLPLPDHTDDDSALVCSCFGVSEQKIRRLIRE
NHLTSAEEVTAYSKAGGGCGSCLAQIEDLIAAVLAETDATPSEDSRNSKGNSQNQTEPSPAAPSPVSATATPPPLTTLQK
IARIQAVLEEEVRPLLLADGGDVELYDLEGDQVWVRLKGSCTTCPSQQNTLRLLIETRLQEQVWPTLTVHAL

Sequences:

>Translated_312_residues
MWDYSEKVLELFYHPKNQGAMDPSSEPGVRVVVGEVGSITCGDALRLYLKVDEASERILAASFQTFGCTSAIASSSALTE
LITGLTLDEALRIDNRQIADYLGGLPPAKMHCSVMGQEALEAAIYSYRGLPLPDHTDDDSALVCSCFGVSEQKIRRLIRE
NHLTSAEEVTAYSKAGGGCGSCLAQIEDLIAAVLAETDATPSEDSRNSKGNSQNQTEPSPAAPSPVSATATPPPLTTLQK
IARIQAVLEEEVRPLLLADGGDVELYDLEGDQVWVRLKGSCTTCPSQQNTLRLLIETRLQEQVWPTLTVHAL
>Mature_312_residues
MWDYSEKVLELFYHPKNQGAMDPSSEPGVRVVVGEVGSITCGDALRLYLKVDEASERILAASFQTFGCTSAIASSSALTE
LITGLTLDEALRIDNRQIADYLGGLPPAKMHCSVMGQEALEAAIYSYRGLPLPDHTDDDSALVCSCFGVSEQKIRRLIRE
NHLTSAEEVTAYSKAGGGCGSCLAQIEDLIAAVLAETDATPSEDSRNSKGNSQNQTEPSPAAPSPVSATATPPPLTTLQK
IARIQAVLEEEVRPLLLADGGDVELYDLEGDQVWVRLKGSCTTCPSQQNTLRLLIETRLQEQVWPTLTVHAL

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=125, Percent_Identity=44.8, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI24307953, Length=126, Percent_Identity=44.4444444444444, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788878, Length=126, Percent_Identity=46.031746031746, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17543474, Length=125, Percent_Identity=45.6, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6325122, Length=125, Percent_Identity=47.2, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324800, Length=125, Percent_Identity=45.6, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355597, Length=125, Percent_Identity=44.8, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 33605; Mature: 33605

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDYSEKVLELFYHPKNQGAMDPSSEPGVRVVVGEVGSITCGDALRLYLKVDEASERILA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEECCCEEEEEEEECCHHHHHHH
ASFQTFGCTSAIASSSALTELITGLTLDEALRIDNRQIADYLGGLPPAKMHCSVMGQEAL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHH
EAAIYSYRGLPLPDHTDDDSALVCSCFGVSEQKIRRLIRENHLTSAEEVTAYSKAGGGCG
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
SCLAQIEDLIAAVLAETDATPSEDSRNSKGNSQNQTEPSPAAPSPVSATATPPPLTTLQK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
IARIQAVLEEEVRPLLLADGGDVELYDLEGDQVWVRLKGSCTTCPSQQNTLRLLIETRLQ
HHHHHHHHHHCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
EQVWPTLTVHAL
HHHCCCEEEEEC
>Mature Secondary Structure
MWDYSEKVLELFYHPKNQGAMDPSSEPGVRVVVGEVGSITCGDALRLYLKVDEASERILA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEECCCEEEEEEEECCHHHHHHH
ASFQTFGCTSAIASSSALTELITGLTLDEALRIDNRQIADYLGGLPPAKMHCSVMGQEAL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHH
EAAIYSYRGLPLPDHTDDDSALVCSCFGVSEQKIRRLIRENHLTSAEEVTAYSKAGGGCG
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
SCLAQIEDLIAAVLAETDATPSEDSRNSKGNSQNQTEPSPAAPSPVSATATPPPLTTLQK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
IARIQAVLEEEVRPLLLADGGDVELYDLEGDQVWVRLKGSCTTCPSQQNTLRLLIETRLQ
HHHHHHHHHHCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
EQVWPTLTVHAL
HHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]