Click here to switch to the map view.

The map label for this gene is truA

Identifier: 86606117

GI number: 86606117

Start: 1454899

End: 1455762

Strand: Reverse

Name: truA

Synonym: CYA_1449

Alternate gene names: 86606117

Gene position: 1455762-1454899 (Counterclockwise)

Preceding gene: 86606118

Following gene: 86606116

Centisome position: 49.64

GC content: 65.62

Gene sequence:

>864_bases
ATGCAGAGCCGTCGCATTGCCCTGAAAATTCAGTACCTCGGCAGCGGCTTTCGCGGCTGGCAGCGGCAAGCGGGCCACCG
CACCGTGCAAGGGGTACTGGAGGAGGCGATTGCGTTGCGGGCCGGGCACCCGGTGGCCCTCTACGGGGCAGGTCGCACCG
ATACGGGGGTTCATGCCTCGGGCCAGGTGGCCCACTTCGACACCACCTCTGGCATCCCGCCGACGGAGTGGGCGCGGGTG
CTCAACGACTGTCTGCCGGAAGATGTGGTGGTATTGGCTTCCGCTCAGGTGCCCGATTTCTGGCATGCCCGCTTTTCGGC
TCGCTGGCGCCGCTATCGCTACTTGATCCTGAACCGGGAGCTGCCGGATGTGTTCTGGCGCCTCTTTAGCTGGCAGGTGC
GCTGGCCTTTGCAGGTGGAGCCGATGGCGGAGGCCCTCAACTCTCTTCTGGGCGAGCACAGCCTAGAAGCCTTTCGCCGC
CGGGGATCCGACCGGCCCCACTCGCGGGTGCAGGTGCAGGAGGTGCTCTGCCAGCGGCTGGGGGATCTCATCAGCATCGA
GGTGCAGGCCAGCGGCTTTCTCTACCGCATGATGCGGCTGCTGGTGGGATCCCTGGAGGTGGTGGGCCGTGGCGAGATCA
GCCCACAACAATTTGTCCACCTCTGGCAACACAACGACTGGAGCCGGATGCGCACCCGCTACAGCGCCCCTCCGCAAGGG
CTGTGCCTGGTGGATGTGGGCTATGCTGGGGATCCCTTTGGCCGCCGCTTCGCTCACTCGCCGCCCTTTTGGGCCTTGCC
CCTGCAGGGGTTGCCCAAAACCCCCGATTGGTCGGCGGCTTTGTGTGTTTCTTCCCTGGCCTAG

Upstream 100 bases:

>100_bases
GCTACCGCGATCGGCCTGGGGGCTACACCCGCATCCTGCGCACGGTGCGTCGACGGGGCGACGGGGCAGAAATGGCGGTC
ATCGAGTTGGTGTAGGCCCC

Downstream 100 bases:

>100_bases
GCGTTTTCTTGTCCATTCCATTCTGCGGTTGATGTCATCCTTGGCTGCCATGAACACAACTCCCGTTCCTCCCCCCGAAA
CTCTAACCCCTCGCTGGTAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MQSRRIALKIQYLGSGFRGWQRQAGHRTVQGVLEEAIALRAGHPVALYGAGRTDTGVHASGQVAHFDTTSGIPPTEWARV
LNDCLPEDVVVLASAQVPDFWHARFSARWRRYRYLILNRELPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRR
RGSDRPHSRVQVQEVLCQRLGDLISIEVQASGFLYRMMRLLVGSLEVVGRGEISPQQFVHLWQHNDWSRMRTRYSAPPQG
LCLVDVGYAGDPFGRRFAHSPPFWALPLQGLPKTPDWSAALCVSSLA

Sequences:

>Translated_287_residues
MQSRRIALKIQYLGSGFRGWQRQAGHRTVQGVLEEAIALRAGHPVALYGAGRTDTGVHASGQVAHFDTTSGIPPTEWARV
LNDCLPEDVVVLASAQVPDFWHARFSARWRRYRYLILNRELPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRR
RGSDRPHSRVQVQEVLCQRLGDLISIEVQASGFLYRMMRLLVGSLEVVGRGEISPQQFVHLWQHNDWSRMRTRYSAPPQG
LCLVDVGYAGDPFGRRFAHSPPFWALPLQGLPKTPDWSAALCVSSLA
>Mature_287_residues
MQSRRIALKIQYLGSGFRGWQRQAGHRTVQGVLEEAIALRAGHPVALYGAGRTDTGVHASGQVAHFDTTSGIPPTEWARV
LNDCLPEDVVVLASAQVPDFWHARFSARWRRYRYLILNRELPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRR
RGSDRPHSRVQVQEVLCQRLGDLISIEVQASGFLYRMMRLLVGSLEVVGRGEISPQQFVHLWQHNDWSRMRTRYSAPPQG
LCLVDVGYAGDPFGRRFAHSPPFWALPLQGLPKTPDWSAALCVSSLA

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=33.2129963898917, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1788657, Length=244, Percent_Identity=37.7049180327869, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32560; Mature: 32560

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSRRIALKIQYLGSGFRGWQRQAGHRTVQGVLEEAIALRAGHPVALYGAGRTDTGVHAS
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
GQVAHFDTTSGIPPTEWARVLNDCLPEDVVVLASAQVPDFWHARFSARWRRYRYLILNRE
CCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEECCC
LPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRRRGSDRPHSRVQVQEVLCQRL
HHHHHHHHHHHEECCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GDLISIEVQASGFLYRMMRLLVGSLEVVGRGEISPQQFVHLWQHNDWSRMRTRYSAPPQG
CCEEEEEEECCHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCC
LCLVDVGYAGDPFGRRFAHSPPFWALPLQGLPKTPDWSAALCVSSLA
EEEEEECCCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MQSRRIALKIQYLGSGFRGWQRQAGHRTVQGVLEEAIALRAGHPVALYGAGRTDTGVHAS
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
GQVAHFDTTSGIPPTEWARVLNDCLPEDVVVLASAQVPDFWHARFSARWRRYRYLILNRE
CCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEECCC
LPDVFWRLFSWQVRWPLQVEPMAEALNSLLGEHSLEAFRRRGSDRPHSRVQVQEVLCQRL
HHHHHHHHHHHEECCCEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GDLISIEVQASGFLYRMMRLLVGSLEVVGRGEISPQQFVHLWQHNDWSRMRTRYSAPPQG
CCEEEEEEECCHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCC
LCLVDVGYAGDPFGRRFAHSPPFWALPLQGLPKTPDWSAALCVSSLA
EEEEEECCCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]