Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 86606113

GI number: 86606113

Start: 1452529

End: 1453614

Strand: Reverse

Name: prfA

Synonym: CYA_1445

Alternate gene names: 86606113

Gene position: 1453614-1452529 (Counterclockwise)

Preceding gene: 86606114

Following gene: 86606112

Centisome position: 49.56

GC content: 61.79

Gene sequence:

>1086_bases
ATGGCCTCTGCTCAACTCATCGAGCAGCTTAAAAACGTTGAAGCCACCTTTCAGGAGCTGACGGTACGTCTGGCCGATCC
CGATGTGGCGACCGATCCGGCTGAGCTGCAGCGGATTGCCAAGGCCCGCTCCTCCCTAGAAGCCACCGTCCAGACCTTTC
ACGAGTGGCAAAAGCTCACTCGCGAGCTGAAGCAGACGGAAGAGCTGCTGCGGGAGGTGGCTTCCGACGACCCAGAGCTG
GAGGCGATGGCCAGGGCAGAACTGGAGCAACTGCGCGTCCGCCAAGAACAACTGGAGGAAAAGCTGACCCTGCTGCTGCT
GCCCCGGGATCCCAACGACGAGAAAAACATTATGTTGGAAATCCGCGCCGGCACCGGCGGCGAGGAGGCGGCCCTGTGGG
CGGCGGATTTGGCCCGCATGTACACCCGCTACGCCGAGAAGATGGGCTGGTCGGTGCGCCTGGCCAGCCTCTCGGAAGGA
GAGATGGGAGGCTACAAAGAGGCCATCCTGGAAATTCGCGGGGATCAGGTCTACAGCAAGCTGAAGTTCGAGGCCGGCGT
GCATCGGGTGCAGCGGGTGCCCGTGACAGAGGCCCAGGGGCGCGTCCACACCTCGACGGCCACGGTGGCGGTGATGCCGG
AAGTGGACGAGGTGGAGGTGGTGATCGACCCGAAGGACATCGAGATCAAGACGGCGCGCTCCGGCGGGGCGGGCGGCCAA
AACGTGAACAAGGTGGAAACGGCGGTGGACCTCCTCCACAAGCCCACCGGCATCCGCGTGTTTTGTACCGAGGAGCGCTC
CCAGCTGCAGAACCGGCAGCGGGCGATGCAGATTTTGCGGGCCAAGCTCTACGAGATGAAGCTGCAGGAGCAACAGGCCT
CCATCAGCTCCGCCCGTCGCATGCAGGTGGGAACGGGATCGCGCTCGGAAAAAATTCGCACCTACAACTACAAAGACAAC
CGGGTTACCGATCACCGCCTCAACCAGAACTTCCTGCTCGAGCCAATTTTGGAAGGGGAGCTGGACGAGTTGATCCAGGC
TTGTATTGGCCTATACCAAAAAGAGCTGTTGGCGGAGCTATCCTGA

Upstream 100 bases:

>100_bases
TACGGCATGGGCGGCAAGAAAGCTAGGTAGCCGAGCTTCAATCTGAGTTTCTTTTCTGCCTTGCTGGGCATTGCTGTGAG
TCTTCGCTGACACTGGATCT

Downstream 100 bases:

>100_bases
GGCGGCAGAGCTGGTTGCTGCCGAGCTGGCGGTAGAATGAATACCCACATCGAGTGACCTAAGGAATCCTCCCCATGCTG
GCCGAGTACCGCCAACATGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MASAQLIEQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQTFHEWQKLTRELKQTEELLREVASDDPEL
EAMARAELEQLRVRQEQLEEKLTLLLLPRDPNDEKNIMLEIRAGTGGEEAALWAADLARMYTRYAEKMGWSVRLASLSEG
EMGGYKEAILEIRGDQVYSKLKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQ
NVNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRMQVGTGSRSEKIRTYNYKDN
RVTDHRLNQNFLLEPILEGELDELIQACIGLYQKELLAELS

Sequences:

>Translated_361_residues
MASAQLIEQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQTFHEWQKLTRELKQTEELLREVASDDPEL
EAMARAELEQLRVRQEQLEEKLTLLLLPRDPNDEKNIMLEIRAGTGGEEAALWAADLARMYTRYAEKMGWSVRLASLSEG
EMGGYKEAILEIRGDQVYSKLKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQ
NVNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRMQVGTGSRSEKIRTYNYKDN
RVTDHRLNQNFLLEPILEGELDELIQACIGLYQKELLAELS
>Mature_360_residues
ASAQLIEQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQTFHEWQKLTRELKQTEELLREVASDDPELE
AMARAELEQLRVRQEQLEEKLTLLLLPRDPNDEKNIMLEIRAGTGGEEAALWAADLARMYTRYAEKMGWSVRLASLSEGE
MGGYKEAILEIRGDQVYSKLKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQN
VNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARRMQVGTGSRSEKIRTYNYKDNR
VTDHRLNQNFLLEPILEGELDELIQACIGLYQKELLAELS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=45.2145214521452, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI34577120, Length=318, Percent_Identity=37.7358490566038, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI166795305, Length=196, Percent_Identity=43.8775510204082, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=45.2513966480447, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=37.9411764705882, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=303, Percent_Identity=37.953795379538, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=356, Percent_Identity=39.8876404494382, Blast_Score=219, Evalue=8e-58,
Organism=Drosophila melanogaster, GI19921226, Length=326, Percent_Identity=38.6503067484663, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SYNJA (Q2JUK1)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_474876.1
- ProteinModelPortal:   Q2JUK1
- SMR:   Q2JUK1
- STRING:   Q2JUK1
- GeneID:   3898988
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_1445
- TIGR:   CYA_1445
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   SSP321327:CYA_1445-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40842; Mature: 40711

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAQLIEQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQTFHEWQKLT
CCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELKQTEELLREVASDDPELEAMARAELEQLRVRQEQLEEKLTLLLLPRDPNDEKNIMLE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEE
IRAGTGGEEAALWAADLARMYTRYAEKMGWSVRLASLSEGEMGGYKEAILEIRGDQVYSK
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHH
LKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQ
HHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCEEEEECCCCCEEEECCCCCCCCC
NVNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARR
CHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQVGTGSRSEKIRTYNYKDNRVTDHRLNQNFLLEPILEGELDELIQACIGLYQKELLAEL
HCCCCCCCCCCEEEECCCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure 
ASAQLIEQLKNVEATFQELTVRLADPDVATDPAELQRIAKARSSLEATVQTFHEWQKLT
CHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELKQTEELLREVASDDPELEAMARAELEQLRVRQEQLEEKLTLLLLPRDPNDEKNIMLE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEE
IRAGTGGEEAALWAADLARMYTRYAEKMGWSVRLASLSEGEMGGYKEAILEIRGDQVYSK
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHH
LKFEAGVHRVQRVPVTEAQGRVHTSTATVAVMPEVDEVEVVIDPKDIEIKTARSGGAGGQ
HHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCEEEEECCCCCEEEECCCCCCCCC
NVNKVETAVDLLHKPTGIRVFCTEERSQLQNRQRAMQILRAKLYEMKLQEQQASISSARR
CHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQVGTGSRSEKIRTYNYKDNRVTDHRLNQNFLLEPILEGELDELIQACIGLYQKELLAEL
HCCCCCCCCCCEEEECCCCCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA