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The map label for this gene is acnB

Identifier: 86606112

GI number: 86606112

Start: 1449821

End: 1452454

Strand: Reverse

Name: acnB

Synonym: CYA_1444

Alternate gene names: 86606112

Gene position: 1452454-1449821 (Counterclockwise)

Preceding gene: 86606113

Following gene: 86606111

Centisome position: 49.53

GC content: 65.26

Gene sequence:

>2634_bases
ATGCTGGCCGAGTACCGCCAACATGCTGCTGCCCGCGCGGCCCAAGGGATCCCGCCGCTGCCCTTGAACGCCGCCCAGAC
CAGCCAGGTGTGCGAGCTGCTCAAGGATCCCCCCGCCGGCGAAGAGGCCTTTTTGTTGCATCTGCTTACAGAGCGCGTCC
CGCCGGGGGTGGACGAAGCGGCCTATGTGAAGGCGGGCTTTTTGGCCGCTGTGGCTAAGGAAGAAGTGCACTCGCCCCTC
ATTACCCCCCTGCGGGCGGTAGAGCTCTTGGGCACGATGCTGGGGGGCTACAACGTCCAGGTGTTGCTGGAGCTGCTGCA
GGATGAGCGACCGGAAATTGCCGCCGCAGCGGCCCGCGCCCTCAGCCACACGCTGCTGGTTTTCGACGCCTTCCACGACG
TGCTGGAGCTGAGCCAAGCGGGCAACGCCTATGCCCGACAGGTGCTCCACTCTTGGGCGGAAGGGGAGTGGTTCACCGCC
AGGCCGCCCCTGGCCGAGGCGATCACGGCGACGGTGTTCAAAATTGCCGGCGAGATCAACACCGACGACCTCTCCCCGGC
CGCCCACGCCACCACCCGCCCCGATATTCCCCTTCATGCCCAGAGCATGCTGGAATCGCGGGCGCCGGGAGCGATAGCAG
CCATTGCCGAGCTGAAGCGCCGCGGCCATCCGGTGGCCTTTGTGGGGGATGTGGTGGGCACGGGATCTTCCCGCAAGTCC
GCCCTTAATTCCCTGCTCTGGCACATCGGCCACGACATTCCCTATGTGCCCAACAAGCGGGCCGGCGGGGTGATTCTGGG
GGGCACGATCGCGCCCATTTTCTTCACCACCGCCGAAGATGCCGGCGCCCTGCCCATCCAGTGCGATGTCTCCTGCCTGG
AGACGGGCATGGTAGTGACGATCTACCCCTACGAGGGCGTTATCCGCTCTGAGGATGGCCAAGTCCTCACCACCTTTTCC
CTGCAGCCTGTGACGCTGCCCGACGAGGTGCGGGCCGGGGGCCGCATCCCTCTCTTGATTGGCCGGGCCCTTACCGACAA
AACCCGCCAGGCCCTGGGGCTGGATCCCTCCCCCCTGTTCATTCGCCCCACCCCGCCCGCCGACACGGGCAAGGGCTATA
CCCTGGCCCAGAAAATCGTCGGCAAAGCCTGCGGGCTGCCCGGAGTGCGCCCCGGCACCAGTTGCGAGCCGCTGATCACC
ACGGTGGGATCCCAGGACACCACCGGCCCCATGACCCGCGACGAGCTCAAGGAATTGGCCTGTCTGGGCTTCAGCGCCGA
CCTGGTGCTGCAGAGCTTCTGCCACACCGCCGCCTATCCCAAGCCGGTGGATATCCAGGTTCACAAAGAGCTGCCCGACT
TTTTTGCCTCGCGGGGAGGTGTCGTCCTGCGGCCAGGCGACGGCATCATCCACTCCTGGCTCAACCGCATGCTGCTGCCG
GATACTGTTGGCACCGGGGGCGACTCCCACACCCGCTTCCCTTTGGGCATTTCCTTCCCGGCTGGCTCTGGGCTGGTGGC
CTTTGCCGCCGCCATGGGGGTGATGCCCCTGAATATGCCGGAGTCGGTGCTGGTGCGCTTTAAGGGGGAACTGCAGCCCG
GGATCACCCTGCGGGACATTGTCCACGCCATCCCCTACGTGGCCATCCAGCGGGGGCTGCTCACCGTCGAGAAACAGGGC
AAGAAAAACGTCTTTGCCGGGCGAATTATGGAGATCGAGGGCCTGCCCAACTTGAAGGTGGAGCAAGCCTTCGAGTTCAC
CGACGCCTCGGCGGAACGCAACTGCGCCGCTTGCACCATCCAGCTGAGCGTCGAGACGGTTGCCGAGTACCTGCGCTCCA
ACGTGGTGCTGCTGAAAAACCTGATCGCCCGCGGCTATCAGGATGCGAGAACCCTGCTGCGGCGCATCGCCAAGATGGAG
GCCTGGCTGGCCAACCCCACCCTGATGGAGGCGGATCCCGACGCCGAATACGCCGCCGTCATCGAGATTGATCTCAGCAC
CATCACCGAGCCAATTGTCTGTGCCCCCAACGATCCCGACAAGGTGATGACCCTGAGCGAAGTCCACGCCCAGCACGCGG
CCGGCAACCCCGACACACCCATCCACATTGACGAGGTGTTCATTGGCTCCTGCATGACCAACATTGGCCACTACCGCGCC
GCCGCCAAGATTTTGGAAGGCCAGGGGCGGGCCAAGGTGCGCCAATTTTGGATCTGTCCTCCCACCCGCATGGACGAGGC
GCAACTGCGCGCCGAAGGGGTGTACGGCACCTTTGCCGCTGCCGGGGCGAGGACGGAGATGCCCGGCTGCAGCCTGTGCA
TGGGCAACCAGGCGCGGGTGGCAGACGGGGCCACGGTTATGTCCACCTCCACCCGCAACTTCAACAACCGCATGGGCAAA
GACGCGCGGGTCTATCTGGGATCCGCCGAGCTGGCTGCCGCCTGCGCCCTCTTGGGCCGGATCCCTACCCCTGAGGAATA
CCGGGAGATCGTCGCCACCAAGATCCAGCCCTTTGCCGGCGAGCTGTACCGCTACCTCAACTTCCACGAGATCCCCGGCT
TTGAGGACGAAGGGCGGGTTATCCCCCGCGAGCAAATGCCCAAGCTGGAGGAAGCCCTCGGGATCCGTCCCTAG

Upstream 100 bases:

>100_bases
AAGAGCTGTTGGCGGAGCTATCCTGAGGCGGCAGAGCTGGTTGCTGCCGAGCTGGCGGTAGAATGAATACCCACATCGAG
TGACCTAAGGAATCCTCCCC

Downstream 100 bases:

>100_bases
ACCCAGGGGGGCTTGGCAGCTTGGGCTGCCCTGCTACCATGGCAAGGTGGTGGCCTTAGCCTTGGAGAAACCCTGATGGC
CCCGCCTCGAAAACTCGCCC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 877; Mature: 877

Protein sequence:

>877_residues
MLAEYRQHAAARAAQGIPPLPLNAAQTSQVCELLKDPPAGEEAFLLHLLTERVPPGVDEAAYVKAGFLAAVAKEEVHSPL
ITPLRAVELLGTMLGGYNVQVLLELLQDERPEIAAAAARALSHTLLVFDAFHDVLELSQAGNAYARQVLHSWAEGEWFTA
RPPLAEAITATVFKIAGEINTDDLSPAAHATTRPDIPLHAQSMLESRAPGAIAAIAELKRRGHPVAFVGDVVGTGSSRKS
ALNSLLWHIGHDIPYVPNKRAGGVILGGTIAPIFFTTAEDAGALPIQCDVSCLETGMVVTIYPYEGVIRSEDGQVLTTFS
LQPVTLPDEVRAGGRIPLLIGRALTDKTRQALGLDPSPLFIRPTPPADTGKGYTLAQKIVGKACGLPGVRPGTSCEPLIT
TVGSQDTTGPMTRDELKELACLGFSADLVLQSFCHTAAYPKPVDIQVHKELPDFFASRGGVVLRPGDGIIHSWLNRMLLP
DTVGTGGDSHTRFPLGISFPAGSGLVAFAAAMGVMPLNMPESVLVRFKGELQPGITLRDIVHAIPYVAIQRGLLTVEKQG
KKNVFAGRIMEIEGLPNLKVEQAFEFTDASAERNCAACTIQLSVETVAEYLRSNVVLLKNLIARGYQDARTLLRRIAKME
AWLANPTLMEADPDAEYAAVIEIDLSTITEPIVCAPNDPDKVMTLSEVHAQHAAGNPDTPIHIDEVFIGSCMTNIGHYRA
AAKILEGQGRAKVRQFWICPPTRMDEAQLRAEGVYGTFAAAGARTEMPGCSLCMGNQARVADGATVMSTSTRNFNNRMGK
DARVYLGSAELAAACALLGRIPTPEEYREIVATKIQPFAGELYRYLNFHEIPGFEDEGRVIPREQMPKLEEALGIRP

Sequences:

>Translated_877_residues
MLAEYRQHAAARAAQGIPPLPLNAAQTSQVCELLKDPPAGEEAFLLHLLTERVPPGVDEAAYVKAGFLAAVAKEEVHSPL
ITPLRAVELLGTMLGGYNVQVLLELLQDERPEIAAAAARALSHTLLVFDAFHDVLELSQAGNAYARQVLHSWAEGEWFTA
RPPLAEAITATVFKIAGEINTDDLSPAAHATTRPDIPLHAQSMLESRAPGAIAAIAELKRRGHPVAFVGDVVGTGSSRKS
ALNSLLWHIGHDIPYVPNKRAGGVILGGTIAPIFFTTAEDAGALPIQCDVSCLETGMVVTIYPYEGVIRSEDGQVLTTFS
LQPVTLPDEVRAGGRIPLLIGRALTDKTRQALGLDPSPLFIRPTPPADTGKGYTLAQKIVGKACGLPGVRPGTSCEPLIT
TVGSQDTTGPMTRDELKELACLGFSADLVLQSFCHTAAYPKPVDIQVHKELPDFFASRGGVVLRPGDGIIHSWLNRMLLP
DTVGTGGDSHTRFPLGISFPAGSGLVAFAAAMGVMPLNMPESVLVRFKGELQPGITLRDIVHAIPYVAIQRGLLTVEKQG
KKNVFAGRIMEIEGLPNLKVEQAFEFTDASAERNCAACTIQLSVETVAEYLRSNVVLLKNLIARGYQDARTLLRRIAKME
AWLANPTLMEADPDAEYAAVIEIDLSTITEPIVCAPNDPDKVMTLSEVHAQHAAGNPDTPIHIDEVFIGSCMTNIGHYRA
AAKILEGQGRAKVRQFWICPPTRMDEAQLRAEGVYGTFAAAGARTEMPGCSLCMGNQARVADGATVMSTSTRNFNNRMGK
DARVYLGSAELAAACALLGRIPTPEEYREIVATKIQPFAGELYRYLNFHEIPGFEDEGRVIPREQMPKLEEALGIRP
>Mature_877_residues
MLAEYRQHAAARAAQGIPPLPLNAAQTSQVCELLKDPPAGEEAFLLHLLTERVPPGVDEAAYVKAGFLAAVAKEEVHSPL
ITPLRAVELLGTMLGGYNVQVLLELLQDERPEIAAAAARALSHTLLVFDAFHDVLELSQAGNAYARQVLHSWAEGEWFTA
RPPLAEAITATVFKIAGEINTDDLSPAAHATTRPDIPLHAQSMLESRAPGAIAAIAELKRRGHPVAFVGDVVGTGSSRKS
ALNSLLWHIGHDIPYVPNKRAGGVILGGTIAPIFFTTAEDAGALPIQCDVSCLETGMVVTIYPYEGVIRSEDGQVLTTFS
LQPVTLPDEVRAGGRIPLLIGRALTDKTRQALGLDPSPLFIRPTPPADTGKGYTLAQKIVGKACGLPGVRPGTSCEPLIT
TVGSQDTTGPMTRDELKELACLGFSADLVLQSFCHTAAYPKPVDIQVHKELPDFFASRGGVVLRPGDGIIHSWLNRMLLP
DTVGTGGDSHTRFPLGISFPAGSGLVAFAAAMGVMPLNMPESVLVRFKGELQPGITLRDIVHAIPYVAIQRGLLTVEKQG
KKNVFAGRIMEIEGLPNLKVEQAFEFTDASAERNCAACTIQLSVETVAEYLRSNVVLLKNLIARGYQDARTLLRRIAKME
AWLANPTLMEADPDAEYAAVIEIDLSTITEPIVCAPNDPDKVMTLSEVHAQHAAGNPDTPIHIDEVFIGSCMTNIGHYRA
AAKILEGQGRAKVRQFWICPPTRMDEAQLRAEGVYGTFAAAGARTEMPGCSLCMGNQARVADGATVMSTSTRNFNNRMGK
DARVYLGSAELAAACALLGRIPTPEEYREIVATKIQPFAGELYRYLNFHEIPGFEDEGRVIPREQMPKLEEALGIRP

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=868, Percent_Identity=65.668202764977, Blast_Score=1122, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=375, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323335, Length=403, Percent_Identity=26.5508684863524, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=370, Percent_Identity=26.2162162162162, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=201, Percent_Identity=31.3432835820896, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571643, Length=415, Percent_Identity=23.3734939759036, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 94358; Mature: 94358

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEYRQHAAARAAQGIPPLPLNAAQTSQVCELLKDPPAGEEAFLLHLLTERVPPGVDEA
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCH
AYVKAGFLAAVAKEEVHSPLITPLRAVELLGTMLGGYNVQVLLELLQDERPEIAAAAARA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHH
LSHTLLVFDAFHDVLELSQAGNAYARQVLHSWAEGEWFTARPPLAEAITATVFKIAGEIN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCC
TDDLSPAAHATTRPDIPLHAQSMLESRAPGAIAAIAELKRRGHPVAFVGDVVGTGSSRKS
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECHHHCCCCHHHH
ALNSLLWHIGHDIPYVPNKRAGGVILGGTIAPIFFTTAEDAGALPIQCDVSCLETGMVVT
HHHHHHHHHCCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCEEEECHHHHCCCCEEE
IYPYEGVIRSEDGQVLTTFSLQPVTLPDEVRAGGRIPLLIGRALTDKTRQALGLDPSPLF
EECCCCEEECCCCCEEEEEECCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHCCCCCCEE
IRPTPPADTGKGYTLAQKIVGKACGLPGVRPGTSCEPLITTVGSQDTTGPMTRDELKELA
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHH
CLGFSADLVLQSFCHTAAYPKPVDIQVHKELPDFFASRGGVVLRPGDGIIHSWLNRMLLP
HCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCC
DTVGTGGDSHTRFPLGISFPAGSGLVAFAAAMGVMPLNMPESVLVRFKGELQPGITLRDI
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCHHHH
VHAIPYVAIQRGLLTVEKQGKKNVFAGRIMEIEGLPNLKVEQAFEFTDASAERNCAACTI
HHHHHHHHHHHCHHEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEE
QLSVETVAEYLRSNVVLLKNLIARGYQDARTLLRRIAKMEAWLANPTLMEADPDAEYAAV
EEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEE
IEIDLSTITEPIVCAPNDPDKVMTLSEVHAQHAAGNPDTPIHIDEVFIGSCMTNIGHYRA
EEEEHHHCCCCEEECCCCCHHHEEHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHH
AAKILEGQGRAKVRQFWICPPTRMDEAQLRAEGVYGTFAAAGARTEMPGCSLCMGNQARV
HHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCHHHHCCCCCCCCCCCEEECCCCEE
ADGATVMSTSTRNFNNRMGKDARVYLGSAELAAACALLGRIPTPEEYREIVATKIQPFAG
CCCCEEEECCCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
ELYRYLNFHEIPGFEDEGRVIPREQMPKLEEALGIRP
HHHHHHCHHCCCCCCCCCCCCCHHHCCCHHHHHCCCC
>Mature Secondary Structure
MLAEYRQHAAARAAQGIPPLPLNAAQTSQVCELLKDPPAGEEAFLLHLLTERVPPGVDEA
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCH
AYVKAGFLAAVAKEEVHSPLITPLRAVELLGTMLGGYNVQVLLELLQDERPEIAAAAARA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHH
LSHTLLVFDAFHDVLELSQAGNAYARQVLHSWAEGEWFTARPPLAEAITATVFKIAGEIN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCC
TDDLSPAAHATTRPDIPLHAQSMLESRAPGAIAAIAELKRRGHPVAFVGDVVGTGSSRKS
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECHHHCCCCHHHH
ALNSLLWHIGHDIPYVPNKRAGGVILGGTIAPIFFTTAEDAGALPIQCDVSCLETGMVVT
HHHHHHHHHCCCCCCCCCCCCCCEEECCEEEEEEEEECCCCCCCEEEECHHHHCCCCEEE
IYPYEGVIRSEDGQVLTTFSLQPVTLPDEVRAGGRIPLLIGRALTDKTRQALGLDPSPLF
EECCCCEEECCCCCEEEEEECCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHCCCCCCEE
IRPTPPADTGKGYTLAQKIVGKACGLPGVRPGTSCEPLITTVGSQDTTGPMTRDELKELA
EECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHH
CLGFSADLVLQSFCHTAAYPKPVDIQVHKELPDFFASRGGVVLRPGDGIIHSWLNRMLLP
HCCCCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCC
DTVGTGGDSHTRFPLGISFPAGSGLVAFAAAMGVMPLNMPESVLVRFKGELQPGITLRDI
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHEECCCCCCCCCHHHH
VHAIPYVAIQRGLLTVEKQGKKNVFAGRIMEIEGLPNLKVEQAFEFTDASAERNCAACTI
HHHHHHHHHHHCHHEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEE
QLSVETVAEYLRSNVVLLKNLIARGYQDARTLLRRIAKMEAWLANPTLMEADPDAEYAAV
EEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEE
IEIDLSTITEPIVCAPNDPDKVMTLSEVHAQHAAGNPDTPIHIDEVFIGSCMTNIGHYRA
EEEEHHHCCCCEEECCCCCHHHEEHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHH
AAKILEGQGRAKVRQFWICPPTRMDEAQLRAEGVYGTFAAAGARTEMPGCSLCMGNQARV
HHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCHHHHCCCCCCCCCCCEEECCCCEE
ADGATVMSTSTRNFNNRMGKDARVYLGSAELAAACALLGRIPTPEEYREIVATKIQPFAG
CCCCEEEECCCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
ELYRYLNFHEIPGFEDEGRVIPREQMPKLEEALGIRP
HHHHHHCHHCCCCCCCCCCCCCHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]