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The map label for this gene is gatA

Identifier: 86606088

GI number: 86606088

Start: 1427888

End: 1429417

Strand: Reverse

Name: gatA

Synonym: CYA_1419

Alternate gene names: 86606088

Gene position: 1429417-1427888 (Counterclockwise)

Preceding gene: 86606089

Following gene: 86606083

Centisome position: 48.74

GC content: 63.2

Gene sequence:

>1530_bases
ATGTCTGTCATCCGTGCCCTCCACAGGCAGTTGGTAACCAAAGAGCGCTCTGCCGAAGAGATCGCCCGCGAGTACCTGGA
GCGCCTGGCCCAACTGGAGCCGCAGCTTAAAAGCTTTATTACTGTAACCGAAGAACTGGCTCTGCAACAGGCCAAGGCGG
TGGACGCCCGCATTCGGGCCGGGGAAGAGATTGGCCCTTTGGCCGGGATCCCGTTAGCTGTCAAAGACAACCTCTGTACC
CAAGGGATCCGCACCACCTGCGCTTCCCGGATGCTGGAGGGGTTCATCCCGCCCTACGAGTCGACGGTGACGGCGCGGCT
GGCGGCTGCCGGCATGGTGACGGTGGGCAAGACCAACCTGGACGAGTTTGCCATGGGCAGTTCTACGGAAAACTCGGCCT
TTCAGCGCACCGCCAACCCCTGGGACCTGACGCGGGTGCCGGGGGGATCCTCAGGCGGGTCGGCGGCGGCGGTGGCGGCA
GACCAGGCGGTGGTGGCCCTGGGATCGGATACGGGGGGATCGATCCGCCAGCCGGCGGCCTTCTGTGGCGTGGTCGGCCT
CAAGCCCACCTATGGGCTGGTCTCCCGCTATGGGCTGGTGGCTTTTGCCTCCTCTTTGGATCAAGTGGGGCCCTTTGGGC
GCACGGTGGAGGACGTGGCCCTGCTGCTGCAGGGGATTGCCGGCCATGACCCCCTGGATTCCACCAGCCTCAAGGTGGAG
ATCCCGGACTACAGCCAGGCTTTGATCCCCGAGATCAAAGGCTTCAAGATTGGAGTCATCCGCGACCTGCTGGGAGAAGG
GTGTGGGGAAGAGACCCGCGCTGCCGTGCAAGCGGCCATCCAACACCTAGAGGAGCTGGGGGCAGAGATCCTGGAGATCG
ACTGTCCTAGCTTTCGCTATGGCCTGGCCACCTATTACATCATTGCCCCTTCGGAAGCTTCTTCCAATCTGGCCCGCTAC
GACGGCGTCAAGTACGGCCTGCGGGAACCCGCCGACTCGCTCTTGGCTATGTACGGCAAAACGCGCGCCCGCGGCTTTGG
GCCGGAGGTGAAGCGGCGCATCATGATCGGCACCTATGCCCTCTCTGCCGGCTACTACGACGCCTACTACCTCAAAGCCC
AGAAGGTGCGCACCCTGATCAAGCAGGATTTCCTCAGGGCCTTCGAGAAGGTGGATGTGCTGGTCAGCCCCACCACCCCC
ACGCCGGCCTTCAAGGCGGGGGAGCGGGAGGATCCCTTGAGTATGTACCTGTGCGACCTGATGACGATCCCGGTCAATTT
GGCGGGCCTGCCGGGGCTGAGCCTGCCCTGTGGCTTTGCCAACGGTCTGCCCATTGGCCTGCAGATCATAGGCAACGTCT
TGCAGGAGAGCAAGGTGCTGCGGGTGGCCTATGCCTATGAGCAGAGCACCGACTGGCACAAGCGGCGACCTCCCCTGGGA
CAGCCCCCCCTTGAGCAGGCCCAGGGAACCGCTCAACAGCCCAAAGCCAAGAGCAAGTCCACCAAGGGGAGCAAAAAATC
CAAAAGCTGA

Upstream 100 bases:

>100_bases
ATCTTTAGGGAGTTAGGGTTGGGAAGGGATCCCACCTAGATTAATCTAGAGGAACTTTGTCTGGCTTCACTGGCATTTGC
CCTTCCCCACGAGTTTGCCT

Downstream 100 bases:

>100_bases
GCCCACTAGGCCATGCCCATCTGGTTGGCTGCCGAGAAGCGCTCCATGAAGCGCAGGAAGCGCTCCCATTCTGCTTCTGT
TTCCATGTCGTAGATCACTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQAKAVDARIRAGEEIGPLAGIPLAVKDNLCT
QGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNLDEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAA
DQAVVALGSDTGGSIRQPAAFCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPLDSTSLKVE
IPDYSQALIPEIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLEELGAEILEIDCPSFRYGLATYYIIAPSEASSNLARY
DGVKYGLREPADSLLAMYGKTRARGFGPEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKQDFLRAFEKVDVLVSPTTP
TPAFKAGEREDPLSMYLCDLMTIPVNLAGLPGLSLPCGFANGLPIGLQIIGNVLQESKVLRVAYAYEQSTDWHKRRPPLG
QPPLEQAQGTAQQPKAKSKSTKGSKKSKS

Sequences:

>Translated_509_residues
MSVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQAKAVDARIRAGEEIGPLAGIPLAVKDNLCT
QGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNLDEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAA
DQAVVALGSDTGGSIRQPAAFCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPLDSTSLKVE
IPDYSQALIPEIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLEELGAEILEIDCPSFRYGLATYYIIAPSEASSNLARY
DGVKYGLREPADSLLAMYGKTRARGFGPEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKQDFLRAFEKVDVLVSPTTP
TPAFKAGEREDPLSMYLCDLMTIPVNLAGLPGLSLPCGFANGLPIGLQIIGNVLQESKVLRVAYAYEQSTDWHKRRPPLG
QPPLEQAQGTAQQPKAKSKSTKGSKKSKS
>Mature_508_residues
SVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQAKAVDARIRAGEEIGPLAGIPLAVKDNLCTQ
GIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNLDEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAAD
QAVVALGSDTGGSIRQPAAFCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPLDSTSLKVEI
PDYSQALIPEIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLEELGAEILEIDCPSFRYGLATYYIIAPSEASSNLARYD
GVKYGLREPADSLLAMYGKTRARGFGPEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKQDFLRAFEKVDVLVSPTTPT
PAFKAGEREDPLSMYLCDLMTIPVNLAGLPGLSLPCGFANGLPIGLQIIGNVLQESKVLRVAYAYEQSTDWHKRRPPLGQ
PPLEQAQGTAQQPKAKSKSTKGSKKSKS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=466, Percent_Identity=40.1287553648069, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI195972892, Length=479, Percent_Identity=26.5135699373695, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI166795287, Length=157, Percent_Identity=35.6687898089172, Blast_Score=73, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=439, Percent_Identity=42.1412300683371, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=489, Percent_Identity=26.9938650306748, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=294, Percent_Identity=30.2721088435374, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=237, Percent_Identity=29.1139240506329, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=340, Percent_Identity=27.6470588235294, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=340, Percent_Identity=27.6470588235294, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=270, Percent_Identity=25.5555555555556, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=464, Percent_Identity=33.8362068965517, Blast_Score=243, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=435, Percent_Identity=32.183908045977, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=236, Percent_Identity=28.8135593220339, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=41.4473684210526, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=27.6892430278884, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24652985, Length=493, Percent_Identity=25.9634888438134, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19922090, Length=493, Percent_Identity=25.9634888438134, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24652981, Length=493, Percent_Identity=25.9634888438134, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24652983, Length=493, Percent_Identity=25.9634888438134, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=26.9155206286837, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24648435, Length=238, Percent_Identity=32.7731092436975, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24648437, Length=238, Percent_Identity=32.7731092436975, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI45550774, Length=238, Percent_Identity=32.7731092436975, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21356731, Length=481, Percent_Identity=22.6611226611227, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648441, Length=179, Percent_Identity=32.9608938547486, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648439, Length=179, Percent_Identity=32.9608938547486, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54662; Mature: 54531

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQAKAVDARIRA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEEIGPLAGIPLAVKDNLCTQGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNL
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEECCCCH
DEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAADQAVVALGSDTGGSIRQPAA
HHHHCCCCCCCHHHHHCCCCCCCEECCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHH
FCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPLDSTSLKVE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
IPDYSQALIPEIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLEELGAEILEIDCPSFRY
CCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHEECCCCCHHH
GLATYYIIAPSEASSNLARYDGVKYGLREPADSLLAMYGKTRARGFGPEVKRRIMIGTYA
CCEEEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHEEEEEEEE
LSAGYYDAYYLKAQKVRTLIKQDFLRAFEKVDVLVSPTTPTPAFKAGEREDPLSMYLCDL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHH
MTIPVNLAGLPGLSLPCGFANGLPIGLQIIGNVLQESKVLRVAYAYEQSTDWHKRRPPLG
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCHHHCCCCCC
QPPLEQAQGTAQQPKAKSKSTKGSKKSKS
CCCHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVIRALHRQLVTKERSAEEIAREYLERLAQLEPQLKSFITVTEELALQQAKAVDARIRA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GEEIGPLAGIPLAVKDNLCTQGIRTTCASRMLEGFIPPYESTVTARLAAAGMVTVGKTNL
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCEEEECCCCH
DEFAMGSSTENSAFQRTANPWDLTRVPGGSSGGSAAAVAADQAVVALGSDTGGSIRQPAA
HHHHCCCCCCCHHHHHCCCCCCCEECCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHH
FCGVVGLKPTYGLVSRYGLVAFASSLDQVGPFGRTVEDVALLLQGIAGHDPLDSTSLKVE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEE
IPDYSQALIPEIKGFKIGVIRDLLGEGCGEETRAAVQAAIQHLEELGAEILEIDCPSFRY
CCCCHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHEECCCCCHHH
GLATYYIIAPSEASSNLARYDGVKYGLREPADSLLAMYGKTRARGFGPEVKRRIMIGTYA
CCEEEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHEEEEEEEE
LSAGYYDAYYLKAQKVRTLIKQDFLRAFEKVDVLVSPTTPTPAFKAGEREDPLSMYLCDL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHH
MTIPVNLAGLPGLSLPCGFANGLPIGLQIIGNVLQESKVLRVAYAYEQSTDWHKRRPPLG
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEHHHCCCCHHHCCCCCC
QPPLEQAQGTAQQPKAKSKSTKGSKKSKS
CCCHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA