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The map label for this gene is ygcM [C]

Identifier: 86605788

GI number: 86605788

Start: 1123531

End: 1124484

Strand: Reverse

Name: ygcM [C]

Synonym: CYA_1099

Alternate gene names: 86605788

Gene position: 1124484-1123531 (Counterclockwise)

Preceding gene: 86605792

Following gene: 86605787

Centisome position: 38.34

GC content: 62.58

Gene sequence:

>954_bases
ATGTCCAGAACCTGTACCATCTACCGCCGCGCCCACTTTGCCGCCAGCCACCGCTACTGGCTGCCGGAGCTCTCCGAAGC
TGAGAACCTGGCCCGCTTCGGCTCCCACGCCCGCTTTCCCGGCCACGGCCACAACTACGTGCTGGAGGTGGGCCTGACGG
GGGAGGTAGACGAGTACGGCATGGTGCTCAACCTCTCCCAGGTCAAGCAGGTAATTCGGGAGCGGGTGATTCAAGAACTG
AACTTCGCCTACCTCAACCAAGTCTGGCCAGAGTTTGCCCAAACCCTGCCCACCACCGAGTTCATTGCCTACACCATCTG
GCAGCGCCTAGCCGACGCGCTGCCGCTCAGCAGTGTGCGCCTCTACGAAGATCCCCAGCTTTGGGCCGAATACCGAGGAG
AAGCGATGCAAGCCTATTTGACCGTTGCCACCCACTTTAGCGCCGCCCACCGCTTGGCCCTCGATCACCTCAGCCTGGAA
GAGAACACTGCCATCTACGGCCTCTGCGCTCGCCCCCACGGCCACGGCCACAACTACGGCCTGGAGATTACCGTCAAGGG
ATCCATCGACCCGCGCACAGGGATGATCGTGGATTTGGCAGCTCTGCAGCGGGTGATTGAGGAGCAGGTGGTGAAGCCCC
TGGATCACACTTTCTTGAACAAAGACGTCCCCTACTTTGCCCAGGTGGTGCCCACCGCCGAAAACATCGCCCTCTACGTC
CAGCGGCTGCTGACTTTGCCCCTGCGCGAGCTGGGGGTGCAACTGCATCGGGTTCGCCTGCAAGAGAGCCCCAACAACAG
CAGCGAAGTCTACGGGGAGTTTCCGCTAGAGGAGCTGGGCGCAGCTACCGCTGCCACCACATACCCCGCGAGCGAGGGCA
AGAGGGATCCCTCCGAAGTGGAGCCCCTGGCCGCCGGCAGGGCTGAGCCAGCCGCCTATGCTGGATCTGGTTAA

Upstream 100 bases:

>100_bases
AGGAAGGCGCGCCGAGCCAGGCGCAGGGATCCCTCGCCGCAGGGGCAGTGCTGGTTTGGAGTTTATGCTGAAGGAGGGAT
CCCTTTCTCTCATAGCCCTC

Downstream 100 bases:

>100_bases
GCTGAGCGGGCAGATTCCCGCCTTTGCCCGCCACCTGCAGCAGGAGTCGAAGGCCAACCAAGAGCGTCTGCGCCTGGCCT
TGCAGGAGTTTGCCCGCCTG

Product: 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; Queuosine Biosynthesis Protein QueD; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSRTCTIYRRAHFAASHRYWLPELSEAENLARFGSHARFPGHGHNYVLEVGLTGEVDEYGMVLNLSQVKQVIRERVIQEL
NFAYLNQVWPEFAQTLPTTEFIAYTIWQRLADALPLSSVRLYEDPQLWAEYRGEAMQAYLTVATHFSAAHRLALDHLSLE
ENTAIYGLCARPHGHGHNYGLEITVKGSIDPRTGMIVDLAALQRVIEEQVVKPLDHTFLNKDVPYFAQVVPTAENIALYV
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELGAATAATTYPASEGKRDPSEVEPLAAGRAEPAAYAGSG

Sequences:

>Translated_317_residues
MSRTCTIYRRAHFAASHRYWLPELSEAENLARFGSHARFPGHGHNYVLEVGLTGEVDEYGMVLNLSQVKQVIRERVIQEL
NFAYLNQVWPEFAQTLPTTEFIAYTIWQRLADALPLSSVRLYEDPQLWAEYRGEAMQAYLTVATHFSAAHRLALDHLSLE
ENTAIYGLCARPHGHGHNYGLEITVKGSIDPRTGMIVDLAALQRVIEEQVVKPLDHTFLNKDVPYFAQVVPTAENIALYV
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELGAATAATTYPASEGKRDPSEVEPLAAGRAEPAAYAGSG
>Mature_316_residues
SRTCTIYRRAHFAASHRYWLPELSEAENLARFGSHARFPGHGHNYVLEVGLTGEVDEYGMVLNLSQVKQVIRERVIQELN
FAYLNQVWPEFAQTLPTTEFIAYTIWQRLADALPLSSVRLYEDPQLWAEYRGEAMQAYLTVATHFSAAHRLALDHLSLEE
NTAIYGLCARPHGHGHNYGLEITVKGSIDPRTGMIVDLAALQRVIEEQVVKPLDHTFLNKDVPYFAQVVPTAENIALYVQ
RLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELGAATAATTYPASEGKRDPSEVEPLAAGRAEPAAYAGSG

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=141, Percent_Identity=46.8085106382979, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532224, Length=121, Percent_Identity=40.495867768595, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115532226, Length=121, Percent_Identity=40.495867768595, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19550074, Length=139, Percent_Identity=44.6043165467626, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585378, Length=139, Percent_Identity=44.6043165467626, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 35482; Mature: 35351

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTCTIYRRAHFAASHRYWLPELSEAENLARFGSHARFPGHGHNYVLEVGLTGEVDEYG
CCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCC
MVLNLSQVKQVIRERVIQELNFAYLNQVWPEFAQTLPTTEFIAYTIWQRLADALPLSSVR
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEE
LYEDPQLWAEYRGEAMQAYLTVATHFSAAHRLALDHLSLEENTAIYGLCARPHGHGHNYG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCC
LEITVKGSIDPRTGMIVDLAALQRVIEEQVVKPLDHTFLNKDVPYFAQVVPTAENIALYV
EEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHH
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELGAATAATTYPASEGKRDPSEV
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHCCHHHHCCCCCCCCCCCHHHC
EPLAAGRAEPAAYAGSG
CHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SRTCTIYRRAHFAASHRYWLPELSEAENLARFGSHARFPGHGHNYVLEVGLTGEVDEYG
CCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCC
MVLNLSQVKQVIRERVIQELNFAYLNQVWPEFAQTLPTTEFIAYTIWQRLADALPLSSVR
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEE
LYEDPQLWAEYRGEAMQAYLTVATHFSAAHRLALDHLSLEENTAIYGLCARPHGHGHNYG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCC
LEITVKGSIDPRTGMIVDLAALQRVIEEQVVKPLDHTFLNKDVPYFAQVVPTAENIALYV
EEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHH
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELGAATAATTYPASEGKRDPSEV
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHCCHHHHCCCCCCCCCCCHHHC
EPLAAGRAEPAAYAGSG
CHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA