Click here to switch to the map view.

The map label for this gene is yfkN [H]

Identifier: 86605754

GI number: 86605754

Start: 1075232

End: 1077295

Strand: Reverse

Name: yfkN [H]

Synonym: CYA_1059

Alternate gene names: 86605754

Gene position: 1077295-1075232 (Counterclockwise)

Preceding gene: 86605755

Following gene: 86605753

Centisome position: 36.73

GC content: 64.1

Gene sequence:

>2064_bases
GTGGTCATGAAACCAGCTTGTCGTGCTCGAGGGGCTGCCCCTGCTGAGGGAGGGCTTCCAGCTTGGCTGAACGCTCTGGC
CAAGTTCCTGCGGGTTGGGCTGTGCTGCTTGCTGCTGAGCCTGGCCAGCGCCTGCGGTACCACCGACAGTAGCTCAGTTC
CTCCAGCCGGCGGCGGGGCAGGGTTTATCGCCTCAGATCCCAACGTTAGGCTCTCCTCTCAGCCGCCCGCTTTTACCCTG
CAAATCCTACATGCCAGCGACTTGGAAGCCTCGATCGCTGCCGTACAAGATGCGCCGCGCTTCTCGGCGGTGGTGAACGC
TCTGCGCCCCCAATTCCCAAATACGGTGGTGCTCTCCAGCGGCGACAACTACATTCCCAGCCCCTTCTACAACGCCAGCA
CGGATCCCTCGCTGCGGGGCCGGTATGACCGCACTCCTGGCAAAGCCGACATTGAAATCATCAACGCCATCGGCTTCGAG
GCGGCTGCCTTTGGCAACCATGAATTTGACCAAGGCACACGCCAAATTCGCGACTTGATCCGACCCGACACCAGCCGCGG
CTACGCGGGCGCCCGCTTTCCCTACTTGAGCGCCAACCTCAGCTTCAGCGGCACTGGCGAATTGACCAGCTCTGACTTTG
CTGCCGATGGCCAAGAGGCCAGCTCCATCCGCGGCAAGATCGTCAAGACGGCGGTGATTACGGTGGGAGGGCAGCGGATC
GGCCTGGTGGGTGCCACCACTACGCGCCTGGCCGCCATCTCTAGCCCTGGCCCTGAGGTGAGGGTGGAAGGCAAAGTGGA
CGACACCGACCAGGTGAATGCCGCCCTCATCCAGAACTTCGTCAACCAGCTCACCGGCCAGGGCATTAACAAGATCATCC
TCCTGGCCCACCTGCAGCAGCTCCAAAACGAGGTAACCCTAGCCAGCCAACTGCGGGATGTGGACATCATCATCGCCGGC
GGCTCCCACCGGGTGATCGCCAAGCCCACCGACCGCCTGCGCCCTGAAGATGTGCGCACAGGTGACTACCCCATCCTGCG
CTCCTCGGCCAGCGGCCAACCCGTGGCAGTGGTGAACACCGGCGCCAACTACCGCTACGTGGGCCGCTTGGTGGTCGGTT
TCGACGCCAACGGGGTGATTGCCCGTATCGATCCGGTGAGCGGCGCCTATGCCACCGACGACCAAGGGGTAGCAGCCGTG
CGCGGCACCCCCAACCCCGACGTGGTGCGGATCGCCAACGAGATCGGCAACATCATCCGCACCAAGGATGGCCGCACCTT
TGGCTCTACCACCCAATTCCTCAACGGCCTGCGGGCCGAGGTGCGCACCGAGGAAACCAACCTGGGCAACCTTACGGCGG
ATGCCAATTTGGCCTATGCCAGAACGGTTGACCCCACCACGGTCATCTCCCTCAAAAACGGCGGCGGCATTCGCGCTCCC
ATTGGGGCTACCACCGGCGGTGGCGGCGGCGAGCCGGTGCGGCGGATCCCTCCGCTGGCCAACCCCGATGCCGGCAAGCA
GGCGGGCCAAGTTTCGCAACTGGATATCGAAACTTCCCTTGCCTTTAACAACGGCCTGTCGCTGGTGACGGTTACCGCTG
AGGAGCTGAAGCAACTGATGGAACATGGGGTGGCGCAAAGCCAGCCGGGGGCCACGCCGGGCCGTTTCCCGCAGATCGGC
GGCTTTGCCTTCAGCTTCGATCCCTCGCGGCCACCGGGATCCCGCGTGGTCTCCTTGCGGGTGGACACGCCCACCGGCCG
GGATGTGGTGGTGCGCAACGGCCAGTTGCAAGGGGATCCCCGCCGTACCTTCCGCCTGGTGACGTTGGGCTTTTTGGCCG
ACGGCGGGGATGACTATCCCTTTCCGCGCCTGTCTAACCCCAACCGGGTGAACTTGGCTCCCAGCTCCGGCAACAGCTTC
GATACCCCCGGCAGCGAGCAGGATGCCCTGGCCCGCTACTTGCGGCAGATCGGCGTGTTTACCCAAGCCGACACTCCGGC
TGCCCAAGATACCCGCATCCAGAACCTGTCGGTGCGCCAAGATACGGTGCTGCAGGGCTTGTAG

Upstream 100 bases:

>100_bases
GGATCCCCGGGAAGCTTAACCTGCGCTTGACCTAACCTTAAACAGGTCATAGCGAAATCTCCTGTCTAGCCTTGGTAACG
TGAATGCGGATGCGTCTTGA

Downstream 100 bases:

>100_bases
GCTGGGCTACTCTCAACCGGTGGATGAAGCCAACCGCCCCTCTCCTCTCTGGGGAGAGGGGATGGTTTTAATGAGGAGCC
ATTCAGCATTCTCGACCCAA

Product: 5'-nucleotidase family protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MVMKPACRARGAAPAEGGLPAWLNALAKFLRVGLCCLLLSLASACGTTDSSSVPPAGGGAGFIASDPNVRLSSQPPAFTL
QILHASDLEASIAAVQDAPRFSAVVNALRPQFPNTVVLSSGDNYIPSPFYNASTDPSLRGRYDRTPGKADIEIINAIGFE
AAAFGNHEFDQGTRQIRDLIRPDTSRGYAGARFPYLSANLSFSGTGELTSSDFAADGQEASSIRGKIVKTAVITVGGQRI
GLVGATTTRLAAISSPGPEVRVEGKVDDTDQVNAALIQNFVNQLTGQGINKIILLAHLQQLQNEVTLASQLRDVDIIIAG
GSHRVIAKPTDRLRPEDVRTGDYPILRSSASGQPVAVVNTGANYRYVGRLVVGFDANGVIARIDPVSGAYATDDQGVAAV
RGTPNPDVVRIANEIGNIIRTKDGRTFGSTTQFLNGLRAEVRTEETNLGNLTADANLAYARTVDPTTVISLKNGGGIRAP
IGATTGGGGGEPVRRIPPLANPDAGKQAGQVSQLDIETSLAFNNGLSLVTVTAEELKQLMEHGVAQSQPGATPGRFPQIG
GFAFSFDPSRPPGSRVVSLRVDTPTGRDVVVRNGQLQGDPRRTFRLVTLGFLADGGDDYPFPRLSNPNRVNLAPSSGNSF
DTPGSEQDALARYLRQIGVFTQADTPAAQDTRIQNLSVRQDTVLQGL

Sequences:

>Translated_687_residues
MVMKPACRARGAAPAEGGLPAWLNALAKFLRVGLCCLLLSLASACGTTDSSSVPPAGGGAGFIASDPNVRLSSQPPAFTL
QILHASDLEASIAAVQDAPRFSAVVNALRPQFPNTVVLSSGDNYIPSPFYNASTDPSLRGRYDRTPGKADIEIINAIGFE
AAAFGNHEFDQGTRQIRDLIRPDTSRGYAGARFPYLSANLSFSGTGELTSSDFAADGQEASSIRGKIVKTAVITVGGQRI
GLVGATTTRLAAISSPGPEVRVEGKVDDTDQVNAALIQNFVNQLTGQGINKIILLAHLQQLQNEVTLASQLRDVDIIIAG
GSHRVIAKPTDRLRPEDVRTGDYPILRSSASGQPVAVVNTGANYRYVGRLVVGFDANGVIARIDPVSGAYATDDQGVAAV
RGTPNPDVVRIANEIGNIIRTKDGRTFGSTTQFLNGLRAEVRTEETNLGNLTADANLAYARTVDPTTVISLKNGGGIRAP
IGATTGGGGGEPVRRIPPLANPDAGKQAGQVSQLDIETSLAFNNGLSLVTVTAEELKQLMEHGVAQSQPGATPGRFPQIG
GFAFSFDPSRPPGSRVVSLRVDTPTGRDVVVRNGQLQGDPRRTFRLVTLGFLADGGDDYPFPRLSNPNRVNLAPSSGNSF
DTPGSEQDALARYLRQIGVFTQADTPAAQDTRIQNLSVRQDTVLQGL
>Mature_687_residues
MVMKPACRARGAAPAEGGLPAWLNALAKFLRVGLCCLLLSLASACGTTDSSSVPPAGGGAGFIASDPNVRLSSQPPAFTL
QILHASDLEASIAAVQDAPRFSAVVNALRPQFPNTVVLSSGDNYIPSPFYNASTDPSLRGRYDRTPGKADIEIINAIGFE
AAAFGNHEFDQGTRQIRDLIRPDTSRGYAGARFPYLSANLSFSGTGELTSSDFAADGQEASSIRGKIVKTAVITVGGQRI
GLVGATTTRLAAISSPGPEVRVEGKVDDTDQVNAALIQNFVNQLTGQGINKIILLAHLQQLQNEVTLASQLRDVDIIIAG
GSHRVIAKPTDRLRPEDVRTGDYPILRSSASGQPVAVVNTGANYRYVGRLVVGFDANGVIARIDPVSGAYATDDQGVAAV
RGTPNPDVVRIANEIGNIIRTKDGRTFGSTTQFLNGLRAEVRTEETNLGNLTADANLAYARTVDPTTVISLKNGGGIRAP
IGATTGGGGGEPVRRIPPLANPDAGKQAGQVSQLDIETSLAFNNGLSLVTVTAEELKQLMEHGVAQSQPGATPGRFPQIG
GFAFSFDPSRPPGSRVVSLRVDTPTGRDVVVRNGQLQGDPRRTFRLVTLGFLADGGDDYPFPRLSNPNRVNLAPSSGNSF
DTPGSEQDALARYLRQIGVFTQADTPAAQDTRIQNLSVRQDTVLQGL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=566, Percent_Identity=27.208480565371, Blast_Score=146, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19922446, Length=524, Percent_Identity=25, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24654424, Length=524, Percent_Identity=25, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28573524, Length=573, Percent_Identity=24.2582897033159, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19922444, Length=531, Percent_Identity=24.8587570621469, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI221329836, Length=576, Percent_Identity=24.3055555555556, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24641187, Length=574, Percent_Identity=23.5191637630662, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 72279; Mature: 72279

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMKPACRARGAAPAEGGLPAWLNALAKFLRVGLCCLLLSLASACGTTDSSSVPPAGGGA
CCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFIASDPNVRLSSQPPAFTLQILHASDLEASIAAVQDAPRFSAVVNALRPQFPNTVVLSS
CEEECCCCEEECCCCCEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEC
GDNYIPSPFYNASTDPSLRGRYDRTPGKADIEIINAIGFEAAAFGNHEFDQGTRQIRDLI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHH
RPDTSRGYAGARFPYLSANLSFSGTGELTSSDFAADGQEASSIRGKIVKTAVITVGGQRI
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEEEECCCEE
GLVGATTTRLAAISSPGPEVRVEGKVDDTDQVNAALIQNFVNQLTGQGINKIILLAHLQQ
EEEECCHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LQNEVTLASQLRDVDIIIAGGSHRVIAKPTDRLRPEDVRTGDYPILRSSASGQPVAVVNT
HHHHHHHHHHCCCEEEEEECCCCEEEECCHHCCCCCCCCCCCCCEEECCCCCCCEEEEEC
GANYRYVGRLVVGFDANGVIARIDPVSGAYATDDQGVAAVRGTPNPDVVRIANEIGNIIR
CCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHEE
TKDGRTFGSTTQFLNGLRAEVRTEETNLGNLTADANLAYARTVDPTTVISLKNGGGIRAP
CCCCCCCCCHHHHHHHHHHHHEECCCCCCCEEECCCEEEEEECCCEEEEEEECCCCEECC
IGATTGGGGGEPVRRIPPLANPDAGKQAGQVSQLDIETSLAFNNGLSLVTVTAEELKQLM
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHH
EHGVAQSQPGATPGRFPQIGGFAFSFDPSRPPGSRVVSLRVDTPTGRDVVVRNGQLQGDP
HCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCC
RRTFRLVTLGFLADGGDDYPFPRLSNPNRVNLAPSSGNSFDTPGSEQDALARYLRQIGVF
CCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCE
TQADTPAAQDTRIQNLSVRQDTVLQGL
ECCCCCCCCCCEECCCCCCHHHHHCCC
>Mature Secondary Structure
MVMKPACRARGAAPAEGGLPAWLNALAKFLRVGLCCLLLSLASACGTTDSSSVPPAGGGA
CCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFIASDPNVRLSSQPPAFTLQILHASDLEASIAAVQDAPRFSAVVNALRPQFPNTVVLSS
CEEECCCCEEECCCCCEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEC
GDNYIPSPFYNASTDPSLRGRYDRTPGKADIEIINAIGFEAAAFGNHEFDQGTRQIRDLI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCHHHHHHHHHHHH
RPDTSRGYAGARFPYLSANLSFSGTGELTSSDFAADGQEASSIRGKIVKTAVITVGGQRI
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEEEECCCEE
GLVGATTTRLAAISSPGPEVRVEGKVDDTDQVNAALIQNFVNQLTGQGINKIILLAHLQQ
EEEECCHHHEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LQNEVTLASQLRDVDIIIAGGSHRVIAKPTDRLRPEDVRTGDYPILRSSASGQPVAVVNT
HHHHHHHHHHCCCEEEEEECCCCEEEECCHHCCCCCCCCCCCCCEEECCCCCCCEEEEEC
GANYRYVGRLVVGFDANGVIARIDPVSGAYATDDQGVAAVRGTPNPDVVRIANEIGNIIR
CCCEEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHEE
TKDGRTFGSTTQFLNGLRAEVRTEETNLGNLTADANLAYARTVDPTTVISLKNGGGIRAP
CCCCCCCCCHHHHHHHHHHHHEECCCCCCCEEECCCEEEEEECCCEEEEEEECCCCEECC
IGATTGGGGGEPVRRIPPLANPDAGKQAGQVSQLDIETSLAFNNGLSLVTVTAEELKQLM
CCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHH
EHGVAQSQPGATPGRFPQIGGFAFSFDPSRPPGSRVVSLRVDTPTGRDVVVRNGQLQGDP
HCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCC
RRTFRLVTLGFLADGGDDYPFPRLSNPNRVNLAPSSGNSFDTPGSEQDALARYLRQIGVF
CCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCE
TQADTPAAQDTRIQNLSVRQDTVLQGL
ECCCCCCCCCCEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]