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The map label for this gene is gldA [C]

Identifier: 86605691

GI number: 86605691

Start: 1005261

End: 1006382

Strand: Reverse

Name: gldA [C]

Synonym: CYA_0992

Alternate gene names: 86605691

Gene position: 1006382-1005261 (Counterclockwise)

Preceding gene: 86605697

Following gene: 86605688

Centisome position: 34.32

GC content: 66.22

Gene sequence:

>1122_bases
ATGCTGAGAACTTGTGAATTCGACCTGGCCATTCCGGCCCCAGGCCGGCTATACCGCGGCAGCAGCATCCTCGAGGCTGT
CCCCCTGACAGCTCTGGGATCCCGCGTGTTGGTGATCGGGGGGGAGCGCTCCCTGGCCGTGGCCGAGCTGCCCCTGAAGC
GCAATCTGGGGCGGGATCCCGCCGTTCAGGTTTACTGGAGCACCTATGGTGTCGACTGCAGCGAGTCGGAGCTGGCTCGC
CTACAGGCCCTGGCCACGGCAGAGTCCGTAACCGGCATTGTAGGGGTGGGGGGCGGCAAGGCCCTAGACACGGCCAAGTT
GGTGGCCCATCGCTTGGGGATCCCGGTGGCGACGGTGCCCACCTCCGCCGCCACCTGTGCCGCCTGGAGCGCCCTGTCCA
ACATCTACTCTGACACAGGTGCCTTTCAGCGGGATGTGGCTTTGGATCGGGCACCTGCCGCTTTGATTCTGGACTATGAG
CTCATCCTGCAGGCCCCGGCGCGCACCCTGGTGGCCGGGATTGGAGATGCCTTGGCCAAGTGGTACGAGTCTTCTGTCAG
CAGCGGCGCCTCGGAAGATGCCCTGGTGATCGGAGCTGTGCAGCAGGCCCGCGTTTTGCGGGATTTGCTCTTGCAACAAT
CGGTGGCGGCCCTGCGGGAGCCGGGTGGCACGGCTTGGAAGCAGGTGGTGGATGCCTGCATTTGCCTGGCGGGGATCGTC
GGCGGCATGGGAGGGGCTAAGTGTCGCACCGTGGCCGCCCACGCCGTTCACAACGGCCTAACCCATTTGCCGGGACACCG
GGCCACCCTGCACGGGGAGAAAGTGGCCTTTGGCATTTTGGCCCAGCTCCGCCTAGAGGAGATCCTGCAGGGAAGCCAGT
TGGCGCTGGCCGCCCGCACCCAGTTGCTGGCGTTCTACCGCCAGGTGGGGCTGCCCCTCAACTTGCGGGATCTCCACCTC
GACCACCTGGGATCCGCCGAGCTGCTGCAAGCAGCCCAGGTGGCCTGCCAGCCAGGCTCCGATATCCACCACCTGCCCTT
CCCCGTCAGCCCGGAAGCCCTGCTGGTGGCTCTTACCACCACCGACCTGACCAGCTCCAGCCCCGCCTTGCTGCGGCTTT
AG

Upstream 100 bases:

>100_bases
GTAGCTACACCCTAGATGGGAAAGGCAGAACTTGCGGCTAGAATCGTAGTCTGTATCTATATATATGGACACTGGGCTGA
CCCTATCGCCCCTCTTTGAA

Downstream 100 bases:

>100_bases
GCCCGCCAGCCTCTGCCACCTTAAACTTTGCCGGTACGGGCCTGAGTTTTCCTCACCCGCCGCCGCGCCTCGCCGTAGGC
CCCGGAGGTGGCGCCTGCCA

Product: alcohol dehydrogenase, iron-containing

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLRTCEFDLAIPAPGRLYRGSSILEAVPLTALGSRVLVIGGERSLAVAELPLKRNLGRDPAVQVYWSTYGVDCSESELAR
LQALATAESVTGIVGVGGGKALDTAKLVAHRLGIPVATVPTSAATCAAWSALSNIYSDTGAFQRDVALDRAPAALILDYE
LILQAPARTLVAGIGDALAKWYESSVSSGASEDALVIGAVQQARVLRDLLLQQSVAALREPGGTAWKQVVDACICLAGIV
GGMGGAKCRTVAAHAVHNGLTHLPGHRATLHGEKVAFGILAQLRLEEILQGSQLALAARTQLLAFYRQVGLPLNLRDLHL
DHLGSAELLQAAQVACQPGSDIHHLPFPVSPEALLVALTTTDLTSSSPALLRL

Sequences:

>Translated_373_residues
MLRTCEFDLAIPAPGRLYRGSSILEAVPLTALGSRVLVIGGERSLAVAELPLKRNLGRDPAVQVYWSTYGVDCSESELAR
LQALATAESVTGIVGVGGGKALDTAKLVAHRLGIPVATVPTSAATCAAWSALSNIYSDTGAFQRDVALDRAPAALILDYE
LILQAPARTLVAGIGDALAKWYESSVSSGASEDALVIGAVQQARVLRDLLLQQSVAALREPGGTAWKQVVDACICLAGIV
GGMGGAKCRTVAAHAVHNGLTHLPGHRATLHGEKVAFGILAQLRLEEILQGSQLALAARTQLLAFYRQVGLPLNLRDLHL
DHLGSAELLQAAQVACQPGSDIHHLPFPVSPEALLVALTTTDLTSSSPALLRL
>Mature_373_residues
MLRTCEFDLAIPAPGRLYRGSSILEAVPLTALGSRVLVIGGERSLAVAELPLKRNLGRDPAVQVYWSTYGVDCSESELAR
LQALATAESVTGIVGVGGGKALDTAKLVAHRLGIPVATVPTSAATCAAWSALSNIYSDTGAFQRDVALDRAPAALILDYE
LILQAPARTLVAGIGDALAKWYESSVSSGASEDALVIGAVQQARVLRDLLLQQSVAALREPGGTAWKQVVDACICLAGIV
GGMGGAKCRTVAAHAVHNGLTHLPGHRATLHGEKVAFGILAQLRLEEILQGSQLALAARTQLLAFYRQVGLPLNLRDLHL
DHLGSAELLQAAQVACQPGSDIHHLPFPVSPEALLVALTTTDLTSSSPALLRL

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=367, Percent_Identity=35.6948228882834, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786815, Length=335, Percent_Identity=39.4029850746269, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 39004; Mature: 39004

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTCEFDLAIPAPGRLYRGSSILEAVPLTALGSRVLVIGGERSLAVAELPLKRNLGRDP
CCCEEEEEEECCCCCCEECCCHHHHHHCHHHCCCEEEEEECCCCEEEEECCHHHCCCCCC
AVQVYWSTYGVDCSESELARLQALATAESVTGIVGVGGGKALDTAKLVAHRLGIPVATVP
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHCCCEEECC
TSAATCAAWSALSNIYSDTGAFQRDVALDRAPAALILDYELILQAPARTLVAGIGDALAK
CCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHH
WYESSVSSGASEDALVIGAVQQARVLRDLLLQQSVAALREPGGTAWKQVVDACICLAGIV
HHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GGMGGAKCRTVAAHAVHNGLTHLPGHRATLHGEKVAFGILAQLRLEEILQGSQLALAART
CCCCCCHHHHHHHHHHHCCHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QLLAFYRQVGLPLNLRDLHLDHLGSAELLQAAQVACQPGSDIHHLPFPVSPEALLVALTT
HHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
TDLTSSSPALLRL
CCCCCCCCCEEEC
>Mature Secondary Structure
MLRTCEFDLAIPAPGRLYRGSSILEAVPLTALGSRVLVIGGERSLAVAELPLKRNLGRDP
CCCEEEEEEECCCCCCEECCCHHHHHHCHHHCCCEEEEEECCCCEEEEECCHHHCCCCCC
AVQVYWSTYGVDCSESELARLQALATAESVTGIVGVGGGKALDTAKLVAHRLGIPVATVP
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHCCCEEECC
TSAATCAAWSALSNIYSDTGAFQRDVALDRAPAALILDYELILQAPARTLVAGIGDALAK
CCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHH
WYESSVSSGASEDALVIGAVQQARVLRDLLLQQSVAALREPGGTAWKQVVDACICLAGIV
HHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GGMGGAKCRTVAAHAVHNGLTHLPGHRATLHGEKVAFGILAQLRLEEILQGSQLALAART
CCCCCCHHHHHHHHHHHCCHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QLLAFYRQVGLPLNLRDLHLDHLGSAELLQAAQVACQPGSDIHHLPFPVSPEALLVALTT
HHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEE
TDLTSSSPALLRL
CCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.5 {glycerol}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]