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The map label for this gene is cobA

Identifier: 86605631

GI number: 86605631

Start: 938697

End: 939455

Strand: Reverse

Name: cobA

Synonym: CYA_0929

Alternate gene names: 86605631

Gene position: 939455-938697 (Counterclockwise)

Preceding gene: 86605632

Following gene: 86605630

Centisome position: 32.03

GC content: 63.11

Gene sequence:

>759_bases
ATGGGCAAAGTGTATCTGGTGGGGGCAGGACCTGGGGATCCGGGACTGCTGACGGTGAAGGGCAAGGCCCTGCTCGCCCA
TGCGGACGTGGTCATCTACGATGCCCTGGTGGGATCCCCCATCTTGGATCTGATCCCCCCCACAGCAGAGCGCATCTATG
CTGGCAAACAGCGGGGGGCTCACACCTTGCCCCAGGAGCAGATCACGGCCCTGTTGATTGAAAAAGCGCAGACACGAGCT
GTAGTGGTGCGGCTCAAGGGCGGGGATCCCTTCATGTTTGGGCGGGGGGGAGAGGAGATGCTGGCCCTGGTGCAGGCCGG
GATCCCGGTGGAGGTGGTGCCGGGAATTACAGCGGGCATGGCCGCCAGCGCCTATGCCGGGATCCCCCTCACCCATCGCG
ACCTCAGCTCCTCGGTTGCTTTTGTTACTGGCCACGAGGCGGCAGGCAAATACCGCCCCCAGGTCAATTGGCGTGCCCTA
GCCGAGGCCGTAGAAACCTTGGTGATCTACATGGGCATGCACAACCTCGGCTACATCGCTGCCGAATTGTTAGCGGGAGG
AAAAGATCCCGCTACCCCAGTCGCCTTGATCCGCTGGGGCACCACCCCGGAGCAAGAGATTCATCTGCTCCGTTTGGAGT
CTCTGGCCGGTCAGCCCTCGCCTGCGGCGCCGCCCGCCATTGTCGTGGTGGGGGCGGTGGTCAATTTGGCCAGCCTGTTG
CCCACCTTGTACCCCCTTGCCCCTTCAGAACCTCAATGA

Upstream 100 bases:

>100_bases
ACCCTGCAGACGGCCTGTCTGACCTGGAAGTAGAGTTGGTTGGTCAAAACTGTCCTGTTCCAGCAGAATAGATATCTCCG
GAAAACCATTGGGACTCGAG

Downstream 100 bases:

>100_bases
ACGCCTTTTTTCGCAAGCTGAGCGCGCTCTTTGCCGCCTACTACGCCTACATGCTGGAGTACCGGGCGGAGCTGCTCCTG
TGGGCCTTGGCCGGATCCCT

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MGKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSPILDLIPPTAERIYAGKQRGAHTLPQEQITALLIEKAQTRA
VVVRLKGGDPFMFGRGGEEMLALVQAGIPVEVVPGITAGMAASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRAL
AEAVETLVIYMGMHNLGYIAAELLAGGKDPATPVALIRWGTTPEQEIHLLRLESLAGQPSPAAPPAIVVVGAVVNLASLL
PTLYPLAPSEPQ

Sequences:

>Translated_252_residues
MGKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSPILDLIPPTAERIYAGKQRGAHTLPQEQITALLIEKAQTRA
VVVRLKGGDPFMFGRGGEEMLALVQAGIPVEVVPGITAGMAASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRAL
AEAVETLVIYMGMHNLGYIAAELLAGGKDPATPVALIRWGTTPEQEIHLLRLESLAGQPSPAAPPAIVVVGAVVNLASLL
PTLYPLAPSEPQ
>Mature_251_residues
GKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSPILDLIPPTAERIYAGKQRGAHTLPQEQITALLIEKAQTRAV
VVRLKGGDPFMFGRGGEEMLALVQAGIPVEVVPGITAGMAASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRALA
EAVETLVIYMGMHNLGYIAAELLAGGKDPATPVALIRWGTTPEQEIHLLRLESLAGQPSPAAPPAIVVVGAVVNLASLLP
TLYPLAPSEPQ

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=47.1074380165289, Blast_Score=213, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6322922, Length=240, Percent_Identity=36.6666666666667, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 26249; Mature: 26118

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSPILDLIPPTAERIYAGKQRGA
CCEEEEEECCCCCCCEEEEECCEEEEECCEEEEHHHHCCCHHHHCCCCHHHHHCCCCCCC
HTLPQEQITALLIEKAQTRAVVVRLKGGDPFMFGRGGEEMLALVQAGIPVEVVPGITAGM
CCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCEEECCCCHHHH
AASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRALAEAVETLVIYMGMHNLGYIA
HHHHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
AELLAGGKDPATPVALIRWGTTPEQEIHLLRLESLAGQPSPAAPPAIVVVGAVVNLASLL
HHHHCCCCCCCCCEEEEEECCCCHHHHHEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHH
PTLYPLAPSEPQ
HHHCCCCCCCCC
>Mature Secondary Structure 
GKVYLVGAGPGDPGLLTVKGKALLAHADVVIYDALVGSPILDLIPPTAERIYAGKQRGA
CEEEEEECCCCCCCEEEEECCEEEEECCEEEEHHHHCCCHHHHCCCCHHHHHCCCCCCC
HTLPQEQITALLIEKAQTRAVVVRLKGGDPFMFGRGGEEMLALVQAGIPVEVVPGITAGM
CCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCEEECCCCHHHH
AASAYAGIPLTHRDLSSSVAFVTGHEAAGKYRPQVNWRALAEAVETLVIYMGMHNLGYIA
HHHHHCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
AELLAGGKDPATPVALIRWGTTPEQEIHLLRLESLAGQPSPAAPPAIVVVGAVVNLASLL
HHHHCCCCCCCCCEEEEEECCCCHHHHHEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHH
PTLYPLAPSEPQ
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]