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The map label for this gene is purU

Identifier: 86605467

GI number: 86605467

Start: 765802

End: 766650

Strand: Reverse

Name: purU

Synonym: CYA_0760

Alternate gene names: 86605467

Gene position: 766650-765802 (Counterclockwise)

Preceding gene: 86605468

Following gene: 86605466

Centisome position: 26.14

GC content: 59.84

Gene sequence:

>849_bases
ATGCCCACGGCCACCCTACTGGTGTCCTGTCCCGATCAAAAAGGCCTGGTAGCCAAGCTCTCCAACTTCATCTACAGCTA
CGACGGCAACATTCTTCACGCTGACCACCACACGGATCTCGAGGCAGGGTTGTTCCTAAGCCGCATCGAGTGGGATTTAG
AAGGCTTTCGGCTAGAACGGTCGGAGATTGTTCCTGCCTTCTCCGGCATTGCCCGCGGGATCCAGGCCAACTGGGAGCTG
CATTTTTCCGATACGCGCCGGCGCATTGCGGTGTGGGTTAGCAAGCAACCCCACTGTCTGTTGGATCTCATCTGGCGGCA
GAGGGCTGGGGAGCTGCCGGCGGAGATCCCTCTGATCATCAGCAACCACCCCGACTTGGAGCCGCTGGCCCGCTCTTTTG
GCATCGACTACTACCACATCCCCGTTTCTCCAGAAAACAGAGCCGAGGCAGAGGCCCGACAACTTGCCTTGCTGCAGGAA
TATCGGATTGACTTAGTGGTGCTGGCCAAATACATGCAGGTCTTGAGTGGGTGGCTGCTCCGCCAGGCGCCCCCGGTGAT
CAACATTCACCACTCTACGCTGCCTGCTTTTGCCGGTGCTAACCCCTACCAGCGTGCCCACCAGCGGGGCGTCAAGATCA
TCGGCGCCACCGCCCACTACGCCACCGAGGAGCTGGATGAAGGGCCAATCATCGAGCAGGACGTAGTGCGGGTGAGTCAC
CGCGACACGGTAGCTGACCTCATCCGCAAGGGCCGCGACGTGGAGCGCCTAGTCTTGGCCCGGGCCGTGCGCTATCACCT
AGAAAATCGCGTCCTGGTGTACTGCAACAAAACGGTGGTGTTTGCCTAG

Upstream 100 bases:

>100_bases
GGTTGTTGCAAGAGCTCAAGACTGAAACCCCCTGGCCAGGGGCGCTTGCCCTCAGACCAATCCAAGTCCTAGGGTAAAGG
GGCGAGCTCTTTGCACTTCT

Downstream 100 bases:

>100_bases
GGAAACTGTCCCTTCCCTTTTGGGTGCAAAGGCCGAGCTCTGGAAAAGAAGCTGCTTTGCCAAACTGGAAAAAAGTCAGG
CTAGAGGTGGGATTATGGCC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPTATLLVSCPDQKGLVAKLSNFIYSYDGNILHADHHTDLEAGLFLSRIEWDLEGFRLERSEIVPAFSGIARGIQANWEL
HFSDTRRRIAVWVSKQPHCLLDLIWRQRAGELPAEIPLIISNHPDLEPLARSFGIDYYHIPVSPENRAEAEARQLALLQE
YRIDLVVLAKYMQVLSGWLLRQAPPVINIHHSTLPAFAGANPYQRAHQRGVKIIGATAHYATEELDEGPIIEQDVVRVSH
RDTVADLIRKGRDVERLVLARAVRYHLENRVLVYCNKTVVFA

Sequences:

>Translated_282_residues
MPTATLLVSCPDQKGLVAKLSNFIYSYDGNILHADHHTDLEAGLFLSRIEWDLEGFRLERSEIVPAFSGIARGIQANWEL
HFSDTRRRIAVWVSKQPHCLLDLIWRQRAGELPAEIPLIISNHPDLEPLARSFGIDYYHIPVSPENRAEAEARQLALLQE
YRIDLVVLAKYMQVLSGWLLRQAPPVINIHHSTLPAFAGANPYQRAHQRGVKIIGATAHYATEELDEGPIIEQDVVRVSH
RDTVADLIRKGRDVERLVLARAVRYHLENRVLVYCNKTVVFA
>Mature_281_residues
PTATLLVSCPDQKGLVAKLSNFIYSYDGNILHADHHTDLEAGLFLSRIEWDLEGFRLERSEIVPAFSGIARGIQANWELH
FSDTRRRIAVWVSKQPHCLLDLIWRQRAGELPAEIPLIISNHPDLEPLARSFGIDYYHIPVSPENRAEAEARQLALLQEY
RIDLVVLAKYMQVLSGWLLRQAPPVINIHHSTLPAFAGANPYQRAHQRGVKIIGATAHYATEELDEGPIIEQDVVRVSHR
DTVADLIRKGRDVERLVLARAVRYHLENRVLVYCNKTVVFA

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=192, Percent_Identity=31.7708333333333, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI209869995, Length=192, Percent_Identity=31.7708333333333, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI209869993, Length=192, Percent_Identity=31.7708333333333, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1787483, Length=273, Percent_Identity=43.2234432234432, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=27.979274611399, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=168, Percent_Identity=34.5238095238095, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582400, Length=204, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32041; Mature: 31910

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTATLLVSCPDQKGLVAKLSNFIYSYDGNILHADHHTDLEAGLFLSRIEWDLEGFRLER
CCCCEEEEECCCCCCHHHHHHHHHEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEECH
SEIVPAFSGIARGIQANWELHFSDTRRRIAVWVSKQPHCLLDLIWRQRAGELPAEIPLII
HHHCHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEE
SNHPDLEPLARSFGIDYYHIPVSPENRAEAEARQLALLQEYRIDLVVLAKYMQVLSGWLL
CCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQAPPVINIHHSTLPAFAGANPYQRAHQRGVKIIGATAHYATEELDEGPIIEQDVVRVSH
HCCCCEEEEECCCCCHHCCCCHHHHHHHCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHC
RDTVADLIRKGRDVERLVLARAVRYHLENRVLVYCNKTVVFA
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEC
>Mature Secondary Structure 
PTATLLVSCPDQKGLVAKLSNFIYSYDGNILHADHHTDLEAGLFLSRIEWDLEGFRLER
CCCEEEEECCCCCCHHHHHHHHHEECCCCEEECCCCCCHHHHHHHHHHHHCCCCEEECH
SEIVPAFSGIARGIQANWELHFSDTRRRIAVWVSKQPHCLLDLIWRQRAGELPAEIPLII
HHHCHHHHHHHHCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEE
SNHPDLEPLARSFGIDYYHIPVSPENRAEAEARQLALLQEYRIDLVVLAKYMQVLSGWLL
CCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQAPPVINIHHSTLPAFAGANPYQRAHQRGVKIIGATAHYATEELDEGPIIEQDVVRVSH
HCCCCEEEEECCCCCHHCCCCHHHHHHHCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHC
RDTVADLIRKGRDVERLVLARAVRYHLENRVLVYCNKTVVFA
CHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]