Click here to switch to the map view.

The map label for this gene is cas3

Identifier: 86605447

GI number: 86605447

Start: 739627

End: 742308

Strand: Reverse

Name: cas3

Synonym: CYA_0735

Alternate gene names: 86605447

Gene position: 742308-739627 (Counterclockwise)

Preceding gene: 86605448

Following gene: 86605446

Centisome position: 25.31

GC content: 56.11

Gene sequence:

>2682_bases
ATGAGCAAGTCCCGATTTTGGGCCAAGACCGGACAGGGACAGTTGCGAGAAGATGGCCGACCTCTGTACCATCCCGTCCT
CTGCCACCTAGCCGATACGGCAGCTGTGGCCATGGAGATCGTGCAAACCTACCTTAGCCCCCTTGCTCGTCAGCAGCTCT
CAGTAGGATTGGGGCTGGAAGGGGATCCCTTAGCTCGCTTTTGCGGGTTCATGGCAGGGTGTCACGACCTAGGCAAAGTC
AGTCCTGCCTTTCAATTTCAAGTGAGCGAGGTGGGAAAAGCGCTGGTGGGATCCCGTCTCTACGGCCTGTGGCGCAGCTA
TCCCAACACTGGCCAAGCAGCCCCTCATGGAACGGTTACCGCCGCAACCTTGCCCGGCTTTCTGGAAGAGCTGGGGCTGC
CCAAGAAACTGGCCCAAAAATTGTCAGCTATTGTCGGCGGTCATCATGGCTTTTTCCCGGGACTTCAGGATATTCAAAGT
CTCTCCCCCGACGATGTCGGTCGAGGGGAGTGGATCCCATTTCGTCGAGAGATCTTCCAGCAACTACAAGATTTTGTTGG
GCTGAAGAGGGAGGGGATCCCTGGCCGCTGCAGCAATTCCGCCGCCATGATCTTGGCCGGCCTGGCAACCGTTGCCGACT
GGATTGCCTCGAATACGGAGAGTTTTCCCTACGCCAACGACGAGCCCTTCGAGACCTACGCTCAAGGAATAGCCGAAAAG
GCCCAACAAGCCCTGAAAGCCCAGGGTTGGATTGCGCCCCCCACAGACAGCCCTCTTACTTTTCAGGCTCTCTTTGACTT
CATCAGCGAGCCGCGACCGCTGCAATTGGCAGCCATTAACCTGAAGGACAGGCTGGCTCCACCCTGCTTGGTCATTGTCG
AGGCCGCCATGGGAGAGGGCAAAACCGAAGCTGCGCTCTACCTGGCCGACCACCTGCAGCATCAAGCCGGAGCCGGTGGC
TTTTACATCGGCCTGCCTACCCAGGCAACCAGCAACGCCATGTTCGAGCGCGTCAAGTCATTTTTGCAAAAGCGCTATCC
CGATGCCATCGTAAACCTAACCCTCAGCCATGGGGCAGCAGCTCTCAAAGAAGATTACCTGGACACCGTCTGTCGCCTGG
ATCAGGTTTATGACCAGGAGGGCAAAGGCGTTTTTGCCAGCGAGTGGCACACTGCCCGCAAGCGGACGCTGCTCAGCCCT
TACGGGGTGGGCACCCCTGATCAGGCTCTGATGGGAGCAGTACGGGCCCGCCACCAGTTTGTCCGCCTGTTTGGTCTGGC
AGGCCGCATCATCATCCTGGACGAGATCCACGCCTACGACCTCTACACCAGCACTCTCCTGGAGCGATTTTTGGAGTGGG
CAGCCGTTTTGGGATCCCCCGTGATCGCCCTCTCGGCCACCTTGCCCGCCGGTACCCGCCAACGATTGCTGGCTGCCTAT
GCCAGAGGCTGTGGACAGCCCCCGCCCGACTCACCTAATGCCCCTTACCCTCGCATCACAGCCCTAGCTTCTGGAGCGGC
CCTAGCTCAGTCTTTTGCTCCTTCCGAGCATGTGCGCCGTCAATTGGGGATCCGCTGGGTCAGGGATGACCAGTGGCCCG
CAGAACTCTCGCAAGTGCTTTCCAAAGGGGGCGGGTGCGTTGCTGTTATCTGCTCAACGGTAGATCGCGCCCAGTGGGTG
TTTCAGCATCTCCAGAAGTATTTTCCGCCGGAAGAATTGGGCCTGTTTCACGGGCGCTTTTTATTCAAAGATCGAGAGCA
GATTGAGAAGCAATGCCTGGAGAGATTCGGCAAAAATGGACATCTCCGTCCCTCTCGTTTTGTTTTGGTGGCAACCCAGG
TCATTGAGCAAAGCTTGGATGTGGATTTTGACTTAATGATCAGCGATCCCGCTCCCATTGATCTTCTCCTACAGCGCTCT
GGGCGGCTCCACCGCCATCCGCGGGACAAGCGACCACCTGAGCTATCTGCTCCCTACTTGTGGATAGTGGAACCAGAGCT
AGCCGAGGAGGGCAAGGCTAATTTTCGGGAGAGCAGCTCTATCTACGATCGGCACATTCTCCTACGCAGTTGGCTGGCTC
TAAGAGAGCGTTGCAACGTGCAACTGCCTGAAGAAATCGACGACCTAATCGAATCGGTATATGACTTGGAGATGCCTATC
CCCGAAGCATTGGAAACCGTTCATGCTCAAGATTGGCGAGAGTCTTTGGAAAAGTACAAAGTTGAAGAAGAGGATGCTAA
AAGATCTCGAGCTAATGAAGTAAAGATCCCACCGCCAAATGCTGATGTGAGGCCCGAACAGTTTACCTACCTAAAAAAAG
AGGATGACGAAAGCACTATCGCTGCCGTTACCAGACTTGGGCAGCCTTCGGTGGCGACAATTTTCTTGCAACAAGGGAAG
ACCGGTTTAGTCTTTCCCGGCTCTCACGATCCAGTGGATCTAGAAACTGAGCCCAGCCTGCCGATGATTCGTAGGCTACT
GGCCCACAGTGTTCGCATTGCTTCCCGCCAGGAGCTTGTAAAGGAGCTGCTGAGGCGGGAAAACCCCAAAACTTGGACAT
CTGCTTTGCTGCGTAACTGTCGCTACGTTGTCTTGAACGCCGAAGGCTGGGCTCAAGTTGGCAAATGGCGACTGCGGTTG
GATCCTAACCTAGGGATCACCATCGAGAAGATGAACAGCTAA

Upstream 100 bases:

>100_bases
GCAGAGGAGCCAGAGTGCTGGAGCCTCCGGATTTGGTGGACATGATGCGGCAAGAGATCTGCGAGATGAGCCGGCAGTAC
GTTGACGAGGAGCCCTAGCG

Downstream 100 bases:

>100_bases
CGTTAGGGAGGAGCTCATGGAAACTCAGTCCTTTAATCTCACCAAAGAAAAGTGGATCCCTGTACTCGATCCCGATTTTC
GAATCCAAGAGTTGTCTTTA

Product: CRISPR-associated helicase Cas3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 893; Mature: 892

Protein sequence:

>893_residues
MSKSRFWAKTGQGQLREDGRPLYHPVLCHLADTAAVAMEIVQTYLSPLARQQLSVGLGLEGDPLARFCGFMAGCHDLGKV
SPAFQFQVSEVGKALVGSRLYGLWRSYPNTGQAAPHGTVTAATLPGFLEELGLPKKLAQKLSAIVGGHHGFFPGLQDIQS
LSPDDVGRGEWIPFRREIFQQLQDFVGLKREGIPGRCSNSAAMILAGLATVADWIASNTESFPYANDEPFETYAQGIAEK
AQQALKAQGWIAPPTDSPLTFQALFDFISEPRPLQLAAINLKDRLAPPCLVIVEAAMGEGKTEAALYLADHLQHQAGAGG
FYIGLPTQATSNAMFERVKSFLQKRYPDAIVNLTLSHGAAALKEDYLDTVCRLDQVYDQEGKGVFASEWHTARKRTLLSP
YGVGTPDQALMGAVRARHQFVRLFGLAGRIIILDEIHAYDLYTSTLLERFLEWAAVLGSPVIALSATLPAGTRQRLLAAY
ARGCGQPPPDSPNAPYPRITALASGAALAQSFAPSEHVRRQLGIRWVRDDQWPAELSQVLSKGGGCVAVICSTVDRAQWV
FQHLQKYFPPEELGLFHGRFLFKDREQIEKQCLERFGKNGHLRPSRFVLVATQVIEQSLDVDFDLMISDPAPIDLLLQRS
GRLHRHPRDKRPPELSAPYLWIVEPELAEEGKANFRESSSIYDRHILLRSWLALRERCNVQLPEEIDDLIESVYDLEMPI
PEALETVHAQDWRESLEKYKVEEEDAKRSRANEVKIPPPNADVRPEQFTYLKKEDDESTIAAVTRLGQPSVATIFLQQGK
TGLVFPGSHDPVDLETEPSLPMIRRLLAHSVRIASRQELVKELLRRENPKTWTSALLRNCRYVVLNAEGWAQVGKWRLRL
DPNLGITIEKMNS

Sequences:

>Translated_893_residues
MSKSRFWAKTGQGQLREDGRPLYHPVLCHLADTAAVAMEIVQTYLSPLARQQLSVGLGLEGDPLARFCGFMAGCHDLGKV
SPAFQFQVSEVGKALVGSRLYGLWRSYPNTGQAAPHGTVTAATLPGFLEELGLPKKLAQKLSAIVGGHHGFFPGLQDIQS
LSPDDVGRGEWIPFRREIFQQLQDFVGLKREGIPGRCSNSAAMILAGLATVADWIASNTESFPYANDEPFETYAQGIAEK
AQQALKAQGWIAPPTDSPLTFQALFDFISEPRPLQLAAINLKDRLAPPCLVIVEAAMGEGKTEAALYLADHLQHQAGAGG
FYIGLPTQATSNAMFERVKSFLQKRYPDAIVNLTLSHGAAALKEDYLDTVCRLDQVYDQEGKGVFASEWHTARKRTLLSP
YGVGTPDQALMGAVRARHQFVRLFGLAGRIIILDEIHAYDLYTSTLLERFLEWAAVLGSPVIALSATLPAGTRQRLLAAY
ARGCGQPPPDSPNAPYPRITALASGAALAQSFAPSEHVRRQLGIRWVRDDQWPAELSQVLSKGGGCVAVICSTVDRAQWV
FQHLQKYFPPEELGLFHGRFLFKDREQIEKQCLERFGKNGHLRPSRFVLVATQVIEQSLDVDFDLMISDPAPIDLLLQRS
GRLHRHPRDKRPPELSAPYLWIVEPELAEEGKANFRESSSIYDRHILLRSWLALRERCNVQLPEEIDDLIESVYDLEMPI
PEALETVHAQDWRESLEKYKVEEEDAKRSRANEVKIPPPNADVRPEQFTYLKKEDDESTIAAVTRLGQPSVATIFLQQGK
TGLVFPGSHDPVDLETEPSLPMIRRLLAHSVRIASRQELVKELLRRENPKTWTSALLRNCRYVVLNAEGWAQVGKWRLRL
DPNLGITIEKMNS
>Mature_892_residues
SKSRFWAKTGQGQLREDGRPLYHPVLCHLADTAAVAMEIVQTYLSPLARQQLSVGLGLEGDPLARFCGFMAGCHDLGKVS
PAFQFQVSEVGKALVGSRLYGLWRSYPNTGQAAPHGTVTAATLPGFLEELGLPKKLAQKLSAIVGGHHGFFPGLQDIQSL
SPDDVGRGEWIPFRREIFQQLQDFVGLKREGIPGRCSNSAAMILAGLATVADWIASNTESFPYANDEPFETYAQGIAEKA
QQALKAQGWIAPPTDSPLTFQALFDFISEPRPLQLAAINLKDRLAPPCLVIVEAAMGEGKTEAALYLADHLQHQAGAGGF
YIGLPTQATSNAMFERVKSFLQKRYPDAIVNLTLSHGAAALKEDYLDTVCRLDQVYDQEGKGVFASEWHTARKRTLLSPY
GVGTPDQALMGAVRARHQFVRLFGLAGRIIILDEIHAYDLYTSTLLERFLEWAAVLGSPVIALSATLPAGTRQRLLAAYA
RGCGQPPPDSPNAPYPRITALASGAALAQSFAPSEHVRRQLGIRWVRDDQWPAELSQVLSKGGGCVAVICSTVDRAQWVF
QHLQKYFPPEELGLFHGRFLFKDREQIEKQCLERFGKNGHLRPSRFVLVATQVIEQSLDVDFDLMISDPAPIDLLLQRSG
RLHRHPRDKRPPELSAPYLWIVEPELAEEGKANFRESSSIYDRHILLRSWLALRERCNVQLPEEIDDLIESVYDLEMPIP
EALETVHAQDWRESLEKYKVEEEDAKRSRANEVKIPPPNADVRPEQFTYLKKEDDESTIAAVTRLGQPSVATIFLQQGKT
GLVFPGSHDPVDLETEPSLPMIRRLLAHSVRIASRQELVKELLRRENPKTWTSALLRNCRYVVLNAEGWAQVGKWRLRLD
PNLGITIEKMNS

Specific function: Unknown

COG id: COG1203

COG function: function code R; Predicted helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789119, Length=851, Percent_Identity=29.8472385428907, Blast_Score=262, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006483
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR006474
- InterPro:   IPR014021 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 99410; Mature: 99278

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSRFWAKTGQGQLREDGRPLYHPVLCHLADTAAVAMEIVQTYLSPLARQQLSVGLGLE
CCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDPLARFCGFMAGCHDLGKVSPAFQFQVSEVGKALVGSRLYGLWRSYPNTGQAAPHGTVT
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
AATLPGFLEELGLPKKLAQKLSAIVGGHHGFFPGLQDIQSLSPDDVGRGEWIPFRREIFQ
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHH
QLQDFVGLKREGIPGRCSNSAAMILAGLATVADWIASNTESFPYANDEPFETYAQGIAEK
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
AQQALKAQGWIAPPTDSPLTFQALFDFISEPRPLQLAAINLKDRLAPPCLVIVEAAMGEG
HHHHHHHCCCCCCCCCCCEEHHHHHHHHCCCCCEEEEEECHHHHCCCCHHHHHHHHHCCC
KTEAALYLADHLQHQAGAGGFYIGLPTQATSNAMFERVKSFLQKRYPDAIVNLTLSHGAA
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHEEEEHHHCHH
ALKEDYLDTVCRLDQVYDQEGKGVFASEWHTARKRTLLSPYGVGTPDQALMGAVRARHQF
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
VRLFGLAGRIIILDEIHAYDLYTSTLLERFLEWAAVLGSPVIALSATLPAGTRQRLLAAY
HHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
ARGCGQPPPDSPNAPYPRITALASGAALAQSFAPSEHVRRQLGIRWVRDDQWPAELSQVL
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCHHHHHHHH
SKGGGCVAVICSTVDRAQWVFQHLQKYFPPEELGLFHGRFLFKDREQIEKQCLERFGKNG
HCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HLRPSRFVLVATQVIEQSLDVDFDLMISDPAPIDLLLQRSGRLHRHPRDKRPPELSAPYL
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEE
WIVEPELAEEGKANFRESSSIYDRHILLRSWLALRERCNVQLPEEIDDLIESVYDLEMPI
EEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PEALETVHAQDWRESLEKYKVEEEDAKRSRANEVKIPPPNADVRPEQFTYLKKEDDESTI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHH
AAVTRLGQPSVATIFLQQGKTGLVFPGSHDPVDLETEPSLPMIRRLLAHSVRIASRQELV
HHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KELLRRENPKTWTSALLRNCRYVVLNAEGWAQVGKWRLRLDPNLGITIEKMNS
HHHHHCCCCCHHHHHHHHCCEEEEECCCCHHHHCCEEEEECCCCCEEEEECCC
>Mature Secondary Structure 
SKSRFWAKTGQGQLREDGRPLYHPVLCHLADTAAVAMEIVQTYLSPLARQQLSVGLGLE
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDPLARFCGFMAGCHDLGKVSPAFQFQVSEVGKALVGSRLYGLWRSYPNTGQAAPHGTVT
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
AATLPGFLEELGLPKKLAQKLSAIVGGHHGFFPGLQDIQSLSPDDVGRGEWIPFRREIFQ
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHH
QLQDFVGLKREGIPGRCSNSAAMILAGLATVADWIASNTESFPYANDEPFETYAQGIAEK
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
AQQALKAQGWIAPPTDSPLTFQALFDFISEPRPLQLAAINLKDRLAPPCLVIVEAAMGEG
HHHHHHHCCCCCCCCCCCEEHHHHHHHHCCCCCEEEEEECHHHHCCCCHHHHHHHHHCCC
KTEAALYLADHLQHQAGAGGFYIGLPTQATSNAMFERVKSFLQKRYPDAIVNLTLSHGAA
CCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHEEEEHHHCHH
ALKEDYLDTVCRLDQVYDQEGKGVFASEWHTARKRTLLSPYGVGTPDQALMGAVRARHQF
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
VRLFGLAGRIIILDEIHAYDLYTSTLLERFLEWAAVLGSPVIALSATLPAGTRQRLLAAY
HHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHH
ARGCGQPPPDSPNAPYPRITALASGAALAQSFAPSEHVRRQLGIRWVRDDQWPAELSQVL
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCHHHHHHHH
SKGGGCVAVICSTVDRAQWVFQHLQKYFPPEELGLFHGRFLFKDREQIEKQCLERFGKNG
HCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HLRPSRFVLVATQVIEQSLDVDFDLMISDPAPIDLLLQRSGRLHRHPRDKRPPELSAPYL
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEE
WIVEPELAEEGKANFRESSSIYDRHILLRSWLALRERCNVQLPEEIDDLIESVYDLEMPI
EEECHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PEALETVHAQDWRESLEKYKVEEEDAKRSRANEVKIPPPNADVRPEQFTYLKKEDDESTI
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHH
AAVTRLGQPSVATIFLQQGKTGLVFPGSHDPVDLETEPSLPMIRRLLAHSVRIASRQELV
HHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KELLRRENPKTWTSALLRNCRYVVLNAEGWAQVGKWRLRLDPNLGITIEKMNS
HHHHHCCCCCHHHHHHHHCCEEEEECCCCHHHHCCEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2005873; 7984428 [H]