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The map label for this gene is lipA-1

Identifier: 86605405

GI number: 86605405

Start: 701015

End: 701869

Strand: Reverse

Name: lipA-1

Synonym: CYA_0692

Alternate gene names: 86605405

Gene position: 701869-701015 (Counterclockwise)

Preceding gene: 86605406

Following gene: 86605403

Centisome position: 23.93

GC content: 61.4

Gene sequence:

>855_bases
ATGTCGGTTAGCCCTCTTCCCAGTTGGGTCAAGCGTTCCATCGGCAAAGCCAGCGAGACTTCAGCGGTGCAGCAGGTGAT
CAAGCAGCGGGGCCTGCACACCATTTGTGAGGAGGGGCGCTGTCCAAATCGAGCCGAGTGCTACAGCCAAAAAACCGCTA
CATTTCTGCTGATGGGAGGGATCTGTACCCGCGCCTGCTCTTTTTGCCAGGTGGCCAGTGGACGGCCCGCACCGTTGGAT
CCCGCAGAGCCGGAGAAAGTGGCCGAGGCGGTTGAGCTGCTGGGGCTCAGCTATGTGGTACTCACCTCGGTGGCCCGCGA
CGATCTGCCCGACCAGGGATCCGGTCGCTTTGTGGAGGTCATGGAGGCAATTCGGCGGCGCTGCCCCGGCACCCAGATCG
AGGTGCTTACCCCCGACTTTCGCGGGGATCGCGAATGCATTGCCCGTGTGGTGGCTGCCCAGCCTGTTTGCTACAACCAC
AACATCGAAACGGTGCGACGCCTCTCCCAGGCAGTACGGCGCAGCTCCGGCTACGACTTGTCCTTGCGGGTTTTGCGAAC
GGTCAAGGAGCTGAATCCTGGATTACCCACCAAGTCTGGGCTGATGCTGGGGCATGGGGAGACCCGCGCAGAAGTGCTGG
AAACTTTCCGGGATCTGCTGGCGGTGGGATGCAATCGTCTGACTCTGGGGCAATACCTACAACCTTCCTTGGCCCATCGC
CCTGTGGAACGCTACTGGACTCCGGAAGAGTTTGCCGAGCTGGGCCAGATAGCCCGCCAGATGGGGTTTCGCCAGGTGCG
CTCAGGGCCATTGGTGCGCAGCTCCTACCACGCTGCCGAAATGGTTGAGAACTAG

Upstream 100 bases:

>100_bases
AGCTTCATCATGTCAGAATGGCAGAGCAGGCGCTGCATCCTTCGGCTGTAGAGGGGCGCAGTCTTGTTGCCGATACCCAG
TTAACGCAACGGTTCTCCCT

Downstream 100 bases:

>100_bases
GCCAGGGAGCTTGGCAGAGAGGCTGTGCAGTCTGTCATAGCCAACCTTTCCCGTCCGATCAAGGCACGAAGGCCCGCTTC
CAGGCCGAACGCTCGGGCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGGICTRACSFCQVASGRPAPLD
PAEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEVMEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNH
NIETVRRLSQAVRRSSGYDLSLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCNRLTLGQYLQPSLAHR
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN

Sequences:

>Translated_284_residues
MSVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGGICTRACSFCQVASGRPAPLD
PAEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEVMEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNH
NIETVRRLSQAVRRSSGYDLSLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCNRLTLGQYLQPSLAHR
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN
>Mature_283_residues
SVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGGICTRACSFCQVASGRPAPLDP
AEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEVMEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNHN
IETVRRLSQAVRRSSGYDLSLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCNRLTLGQYLQPSLAHRP
VERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=43.3098591549296, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=42.4489795918367, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1786846, Length=275, Percent_Identity=47.6363636363636, Blast_Score=269, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=43.006993006993, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=287, Percent_Identity=43.5540069686411, Blast_Score=234, Evalue=8e-63,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=44.5229681978799, Blast_Score=240, Evalue=9e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 31457; Mature: 31325

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ICTRACSFCQVASGRPAPLDPAEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNHNIETVRRLSQAVRRSSGYDL
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCH
SLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCNRLTLGQYLQPSLAHR
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHC
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SVSPLPSWVKRSIGKASETSAVQQVIKQRGLHTICEEGRCPNRAECYSQKTATFLLMGG
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
ICTRACSFCQVASGRPAPLDPAEPEKVAEAVELLGLSYVVLTSVARDDLPDQGSGRFVEV
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
MEAIRRRCPGTQIEVLTPDFRGDRECIARVVAAQPVCYNHNIETVRRLSQAVRRSSGYDL
HHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCH
SLRVLRTVKELNPGLPTKSGLMLGHGETRAEVLETFRDLLAVGCNRLTLGQYLQPSLAHR
HHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHC
PVERYWTPEEFAELGQIARQMGFRQVRSGPLVRSSYHAAEMVEN
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]