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The map label for this gene is purN

Identifier: 86605346

GI number: 86605346

Start: 643786

End: 644448

Strand: Reverse

Name: purN

Synonym: CYA_0632

Alternate gene names: 86605346

Gene position: 644448-643786 (Counterclockwise)

Preceding gene: 86605351

Following gene: 86605345

Centisome position: 21.97

GC content: 62.75

Gene sequence:

>663_bases
ATGGACTTGCCTAACCTCCTCTGGCCAGACCCCGGCGAGATCCCGGCTCCTCCCCAGCCGGGGATCCTGGGCATCTTGGC
TTCCGGCAACGGCAGCAACTTCGAGGCCATCGCCCAAGCCATCGATGCTGGCGAGCTACGGGCGCAAATTGCCGTCGTGA
TCACCAACAATCCAGACGCCTACGTGCGGCAGCGGGCGCGGCGAAGAGGGATCCCTTGTATTCTGCTCAACCATCGGCAC
TATGCCAGCCGGGAGGCGCTGGACGCGGCCATCTTGCAAGTGCTCCAGGAGTATCAGGTGGAGTGGGTGATCATGGCCGG
CTGGATGCGTTTGGTGACCCAAGTGTTGCTGTCTGCCTATCCGGAGCGGGTTTTAAACCTCCACCCCAGCCTCTTGCCCA
GCTTCAAGGGCCTGCGGGCGGTGGAGCAGGCGCTGGAGTACGGCGTGAAAATCACCGGCTGCACCGTCCACCGGGTCACG
TTGGAAATGGATAGCGGCCCCATTGTGGCGCAAGCAGCCGTACCGGTTTTGCCGGAAGATACGGTGGAATCCCTTTACCG
GCGCATCCAGGCCCAGGAACACCGCCTCTACCCGCTGGCCATCCGCCTCTGTTTGGCCGAGGGATCCCGCCTCCCCAAGT
TCTCGGAGAAGCTGCACAATTGA

Upstream 100 bases:

>100_bases
AGCCTAGTTTTTGGACTCTCTCTCCGACAAGAGCAGGTCGCAGCCAAATCCGGCTGGGTCTAAAATAGCGGGGCTGCGGA
CTCTCCCTCGAGTTTTTCGT

Downstream 100 bases:

>100_bases
GGGTGAGCCCGCCGCTGGCGCCCCCTATGGCCGAAATCTCGCCGTTGCTCGTTTTCGACAGCTCTGCGATCCTGGCCGGT
CGCCCGGCTCTGTGGCAGGA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MDLPNLLWPDPGEIPAPPQPGILGILASGNGSNFEAIAQAIDAGELRAQIAVVITNNPDAYVRQRARRRGIPCILLNHRH
YASREALDAAILQVLQEYQVEWVIMAGWMRLVTQVLLSAYPERVLNLHPSLLPSFKGLRAVEQALEYGVKITGCTVHRVT
LEMDSGPIVAQAAVPVLPEDTVESLYRRIQAQEHRLYPLAIRLCLAEGSRLPKFSEKLHN

Sequences:

>Translated_220_residues
MDLPNLLWPDPGEIPAPPQPGILGILASGNGSNFEAIAQAIDAGELRAQIAVVITNNPDAYVRQRARRRGIPCILLNHRH
YASREALDAAILQVLQEYQVEWVIMAGWMRLVTQVLLSAYPERVLNLHPSLLPSFKGLRAVEQALEYGVKITGCTVHRVT
LEMDSGPIVAQAAVPVLPEDTVESLYRRIQAQEHRLYPLAIRLCLAEGSRLPKFSEKLHN
>Mature_220_residues
MDLPNLLWPDPGEIPAPPQPGILGILASGNGSNFEAIAQAIDAGELRAQIAVVITNNPDAYVRQRARRRGIPCILLNHRH
YASREALDAAILQVLQEYQVEWVIMAGWMRLVTQVLLSAYPERVLNLHPSLLPSFKGLRAVEQALEYGVKITGCTVHRVT
LEMDSGPIVAQAAVPVLPEDTVESLYRRIQAQEHRLYPLAIRLCLAEGSRLPKFSEKLHN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=187, Percent_Identity=42.7807486631016, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI209869995, Length=187, Percent_Identity=42.7807486631016, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI209869993, Length=187, Percent_Identity=42.7807486631016, Blast_Score=157, Evalue=7e-39,
Organism=Escherichia coli, GI1788846, Length=200, Percent_Identity=40, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1787483, Length=164, Percent_Identity=33.5365853658537, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=36.8715083798883, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=193, Percent_Identity=29.0155440414508, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=39.7905759162304, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 24419; Mature: 24419

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPNLLWPDPGEIPAPPQPGILGILASGNGSNFEAIAQAIDAGELRAQIAVVITNNPDA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHH
YVRQRARRRGIPCILLNHRHYASREALDAAILQVLQEYQVEWVIMAGWMRLVTQVLLSAY
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
PERVLNLHPSLLPSFKGLRAVEQALEYGVKITGCTVHRVTLEMDSGPIVAQAAVPVLPED
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEEEECCCCEEEECCCCCCCHH
TVESLYRRIQAQEHRLYPLAIRLCLAEGSRLPKFSEKLHN
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MDLPNLLWPDPGEIPAPPQPGILGILASGNGSNFEAIAQAIDAGELRAQIAVVITNNPDA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEECCCHH
YVRQRARRRGIPCILLNHRHYASREALDAAILQVLQEYQVEWVIMAGWMRLVTQVLLSAY
HHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
PERVLNLHPSLLPSFKGLRAVEQALEYGVKITGCTVHRVTLEMDSGPIVAQAAVPVLPED
HHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEEEECCCCEEEECCCCCCCHH
TVESLYRRIQAQEHRLYPLAIRLCLAEGSRLPKFSEKLHN
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]