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The map label for this gene is ribD

Identifier: 86605105

GI number: 86605105

Start: 400816

End: 401931

Strand: Reverse

Name: ribD

Synonym: CYA_0386

Alternate gene names: 86605105

Gene position: 401931-400816 (Counterclockwise)

Preceding gene: 86605108

Following gene: 86605104

Centisome position: 13.7

GC content: 64.61

Gene sequence:

>1116_bases
ATGAACCTGGACGCCGACCGGCAGTGGATGGAACGCTGCCTCTCCTTGGCCCGCTCGACACCACAGCGCCCCTCCCCTAA
CCCGCGGGTAGCCTGCGTTATTGTCCGGGGGGATCAGGTGGTGGGGGAAGGTGTTCATCTGGGAGCCGGCCAGCCCCACG
CTGAGGTCTTGGCTCTGCAGCAGGCGGGATCCCTGGCCAAGGGCGCTACCCTCTACGTCAACCTCGAGCCCTGCAACCAC
TACGGGCGCACCCCTCCCTGTACCGAGGCCATCCTGGCTGCCGGGATCCGGAGGGTGGTGGTGGGAATGCAGGATCCCAA
TCCCCTGGTGGCCGGCAAAGGGATCCGCCGCCTGCAGGAGGCAGGGGTTGAGGTGACGGTGGGGGTGCTGGAGCAGGAAT
GTCAGGAGCTCAACGAAGGCTTTGCCTTCGCCATTGTGCAGAAACGGTGTTTTGGCCTGCTCAAATACGCCATGACCCTA
GACGGCAAGAGCGCCGCCGCCACCGGCCACAGCCGCTGGATCAGCAATCCGCTGGCGCGAGAGTGGGTTCACCGCCAGCG
GGCCTGGCACGATGCAGTCATCGTGGGCTCCGGCACCGTCTGGCACGATAACCCCCACCTTACCTGCCGCCTGCCGGATT
TTCACGGATCCCAGCCCCTGCGAGTGGTTCTCAGCCGCTCTCTGAATCTGCCCCCGCTGGCCCACCTGTGGCGAGCGGAG
GAGGCGCCAACTTTGGTGATTACCGAGGCCACTGAAGCCCATCCCCTCTGGGGATCCCTGGCCAAGCAAGGGGTGGAGCT
GCTGCACCTGGATCAAGTAACCCCGGCTCAAGCGGCGCAGGTTCTCTTTGAGCGAGGCTGTCTGAGTGCCCTCTGGGAAT
GCGGCGGCACCTTGGCCTGGGCAGCCCTTAAAGATGGGGCCATCCAGAAGGTGGCCGCCTTCATCGCTCCCAAGATCTTG
GGCGGGGCCGACGCGCCAACCCCCGTAGCAGGAGAAGGGATCCCGGATGTGAACCAAAGCTGGCAACTGTTGGAGCCGCA
ACTGCAGACTGTGGGGGATAACTGGTTGATAACCGGGAAGATCCTCCCTTCTGGCGCCGACGACTCCGGTGAATGA

Upstream 100 bases:

>100_bases
GTAACCAGCTTACCAAAGCCGCGCCGCAGCCGGCTCCCTGCCAAGAAGCAGCTAGGATCCAAAACACCGCCTGCTAAAGT
GCTCCCCGAAAGCGCCTCCC

Downstream 100 bases:

>100_bases
TTTGTTCTCGATGACTCTCAATTGTCATCCGAGAGAGTTTTAGGTTTTTGAACGAACAAAATCATATCTGCTTAAGGTAA
ACGAGTATACTGCTCAGAAA

Product: riboflavin biosynthesis protein RibD

Products: NA

Alternate protein names: Diaminohydroxyphosphoribosylaminopyrimidine deaminase; DRAP deaminase; Riboflavin-specific deaminase; 5-amino-6-(5-phosphoribosylamino)uracil reductase; HTP reductase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MNLDADRQWMERCLSLARSTPQRPSPNPRVACVIVRGDQVVGEGVHLGAGQPHAEVLALQQAGSLAKGATLYVNLEPCNH
YGRTPPCTEAILAAGIRRVVVGMQDPNPLVAGKGIRRLQEAGVEVTVGVLEQECQELNEGFAFAIVQKRCFGLLKYAMTL
DGKSAAATGHSRWISNPLAREWVHRQRAWHDAVIVGSGTVWHDNPHLTCRLPDFHGSQPLRVVLSRSLNLPPLAHLWRAE
EAPTLVITEATEAHPLWGSLAKQGVELLHLDQVTPAQAAQVLFERGCLSALWECGGTLAWAALKDGAIQKVAAFIAPKIL
GGADAPTPVAGEGIPDVNQSWQLLEPQLQTVGDNWLITGKILPSGADDSGE

Sequences:

>Translated_371_residues
MNLDADRQWMERCLSLARSTPQRPSPNPRVACVIVRGDQVVGEGVHLGAGQPHAEVLALQQAGSLAKGATLYVNLEPCNH
YGRTPPCTEAILAAGIRRVVVGMQDPNPLVAGKGIRRLQEAGVEVTVGVLEQECQELNEGFAFAIVQKRCFGLLKYAMTL
DGKSAAATGHSRWISNPLAREWVHRQRAWHDAVIVGSGTVWHDNPHLTCRLPDFHGSQPLRVVLSRSLNLPPLAHLWRAE
EAPTLVITEATEAHPLWGSLAKQGVELLHLDQVTPAQAAQVLFERGCLSALWECGGTLAWAALKDGAIQKVAAFIAPKIL
GGADAPTPVAGEGIPDVNQSWQLLEPQLQTVGDNWLITGKILPSGADDSGE
>Mature_371_residues
MNLDADRQWMERCLSLARSTPQRPSPNPRVACVIVRGDQVVGEGVHLGAGQPHAEVLALQQAGSLAKGATLYVNLEPCNH
YGRTPPCTEAILAAGIRRVVVGMQDPNPLVAGKGIRRLQEAGVEVTVGVLEQECQELNEGFAFAIVQKRCFGLLKYAMTL
DGKSAAATGHSRWISNPLAREWVHRQRAWHDAVIVGSGTVWHDNPHLTCRLPDFHGSQPLRVVLSRSLNLPPLAHLWRAE
EAPTLVITEATEAHPLWGSLAKQGVELLHLDQVTPAQAAQVLFERGCLSALWECGGTLAWAALKDGAIQKVAAFIAPKIL
GGADAPTPVAGEGIPDVNQSWQLLEPQLQTVGDNWLITGKILPSGADDSGE

Specific function: Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)- pyrimidinedione 5'-phosphate [H]

COG id: COG1985

COG function: function code H; Pyrimidine reductase, riboflavin biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the HTP reductase family [H]

Homologues:

Organism=Escherichia coli, GI1786616, Length=334, Percent_Identity=39.5209580838323, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI145693172, Length=147, Percent_Identity=36.734693877551, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193
- InterPro:   IPR004794
- InterPro:   IPR011549
- InterPro:   IPR002734 [H]

Pfam domain/function: PF00383 dCMP_cyt_deam_1; PF01872 RibD_C [H]

EC number: =3.5.4.26; =1.1.1.193 [H]

Molecular weight: Translated: 39928; Mature: 39928

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDADRQWMERCLSLARSTPQRPSPNPRVACVIVRGDQVVGEGVHLGAGQPHAEVLALQ
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEECCCEEECCCCCHHHHHHHH
QAGSLAKGATLYVNLEPCNHYGRTPPCTEAILAAGIRRVVVGMQDPNPLVAGKGIRRLQE
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHH
AGVEVTVGVLEQECQELNEGFAFAIVQKRCFGLLKYAMTLDGKSAAATGHSRWISNPLAR
CCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHH
EWVHRQRAWHDAVIVGSGTVWHDNPHLTCRLPDFHGSQPLRVVLSRSLNLPPLAHLWRAE
HHHHHHHHHCCEEEEECCEEEECCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHCCC
EAPTLVITEATEAHPLWGSLAKQGVELLHLDQVTPAQAAQVLFERGCLSALWECGGTLAW
CCCEEEEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
AALKDGAIQKVAAFIAPKILGGADAPTPVAGEGIPDVNQSWQLLEPQLQTVGDNWLITGK
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCEEEEEE
ILPSGADDSGE
ECCCCCCCCCC
>Mature Secondary Structure
MNLDADRQWMERCLSLARSTPQRPSPNPRVACVIVRGDQVVGEGVHLGAGQPHAEVLALQ
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCEEECCCEEECCCCCHHHHHHHH
QAGSLAKGATLYVNLEPCNHYGRTPPCTEAILAAGIRRVVVGMQDPNPLVAGKGIRRLQE
HCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHH
AGVEVTVGVLEQECQELNEGFAFAIVQKRCFGLLKYAMTLDGKSAAATGHSRWISNPLAR
CCCEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHH
EWVHRQRAWHDAVIVGSGTVWHDNPHLTCRLPDFHGSQPLRVVLSRSLNLPPLAHLWRAE
HHHHHHHHHCCEEEEECCEEEECCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHCCC
EAPTLVITEATEAHPLWGSLAKQGVELLHLDQVTPAQAAQVLFERGCLSALWECGGTLAW
CCCEEEEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
AALKDGAIQKVAAFIAPKILGGADAPTPVAGEGIPDVNQSWQLLEPQLQTVGDNWLITGK
HHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCHHHHHCCCCEEEEEE
ILPSGADDSGE
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]