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The map label for this gene is pbpG [H]

Identifier: 86604932

GI number: 86604932

Start: 214082

End: 216415

Strand: Reverse

Name: pbpG [H]

Synonym: CYA_0208

Alternate gene names: 86604932

Gene position: 216415-214082 (Counterclockwise)

Preceding gene: 86604933

Following gene: 86604931

Centisome position: 7.38

GC content: 65.68

Gene sequence:

>2334_bases
ATGTCTGCTTCTGAAGACAAGAATTCCCTACGGGGCACCGTCGCTCGCTTCTTCAAGACCCTGGCGAGGCCCTTTCCGGT
CGGGCCTACGGCCCTCCTCAAGCCTGCCGAGCCCCCTCCCCCCAAACGCCCGGTGCGCCTGCAGATCACCTGCCCAGGAG
AGCCTCCCCAGGTGTTTGTCCTGCAGGGATCCGACATTCTTGGTCGCAGCCAGACGGTGGCCACCATCCGCATCCCCGCC
CCTGCGGTGAGCCATCTGCACGCCCGCATTCGCCCGCGTCCGGGCTGGCGTCTGCCTGGCTTGGGGTGGCGGATCCCTCT
GCATCCCCTCCTCTACTGGCTGGGGTGGAACGCTGGTCGCTACCAACTGGAAGATCAAGACTCCACCAACGGCGTCTTTC
GCCTCAAGCCCTTTGGCGGCGCCGAGCGGGTGCGGCGGGTGATCCTGCGCCACGGCCAGCGACTCAGCCTTGGCCCGCCC
CGGGATCCGGAGAGCGTGGTTATTCAGGTGTTGGATCCACCGCCGCCGCAGGTGTACGGGGTGCGCGGCTGCCTGATCCT
GATGGCGCTCTTGGGGCTGGGGGCGAGGCTTTGGATCGGCCATGAGTGGAGCAAGTTCTCAGTAGAACCGCCGCTGGCCG
CCGATCGCAACCCCCTGATCGTCCTGGCCGGGGATCGGCAAACCGAGCTGCGCCGCCACCAGGGGGATCTCTACCGGGAG
CTGCCCAACCTGGAAGCCTTTGGCCCGATTCTGCCCAAAGTGGTGGTGGCCGCCGAAGATCACCGCTTCTACTCCCACTT
CGGGGTCGATCTGCTGGGCATTGCCCGCGCCTTTTGGGTCAACCTGCGCTCCGGGGAGGTGCAGCAGGGGGGCAGCTCCA
TCAGCCAACAACTGGCTCGCACCGTCTTGCGCGACTACACCGGCTCAGGCAACACCTTTGGCCGCAAGCTGCGGGAGGCC
GTCGCCGCCCTCAAGCTGGAACGGCGCTACAGCAAAGACGAAATCCTCACCCTCTACCTCAACAACGTCTACCTGGGCAA
CGGCCTCTACGGCTTTGAGACCGCCGCCCAGTTCTACTTCGGGATCCCCAGCCGCCAGCTCAGCCTTTCCGAAGCCGCCA
CCCTGGCCGCCATTCTGCCCGCCCCCAACGCCTTCAACCCCGTCGCCAACTACGAGGCCGCCGTGCGGGGCCGGGATCGG
GTGTTGGATCGCCTCAGCGAGCTCAAAGTTTTCCCGGAAGAGGAGATTCGGCGGGCCCGGCGCAGCCGCCTCACCCTCAA
CCCCAACCTGCGCTCCCAGACCTCGACAGTTGCCCCCTACTTCTACAGCACCGTCTTCCAGGAGCTGCGGCAGCTTTTGG
GGCAAGATCTCTCGGCAGAGGGCAACTTCGTCATCGAGACGACGCTGCATTTGCCCTACCAGCGGCTGGCGGAGCAGGCC
CTGGCCCAAACGGTGCGCGAGGTGGGATCCCGCCTGGGCTTCAACCAAGGCGCCTTGGTCAGCCTCGACAGCCGCAACGG
CGAGATCCTAGCTCTCGTGGGCGGGATCGACTACACCGCCAGCCCCTTTAACCGTGCCACCCAAGCCCAACGGCAGCCCG
GATCCACCTTCAAAGTCTTCGCCTATGCCGCTGCCCTCAGCCAGGGGATCCCGCTGTCGCAGGTGCTCTCCTGTGAGCCG
CTTACTTGGGGCAACCTCACCTTTCAGGGCTGCCGCTCGGGAAGTGCCCCCATGGACTTGGCCCGCGGCCTGATCCTCTC
CGAAAACGTGATCGCCCTGCGGCTGGCCCAACAGGTAGGGCTGGAGCGGGTGGTGGCCATGGCGCGGCAAATGGGCATCC
AATCCCCCTTACAGCCCTATCCTTCCCTGGTTCTCGGCACGATGGAGGTGAACCTGCTGGAGCTGACCGCGGCCTTTGCC
CCTTTGGCCAATCGCGGCCTTTGGTCTAGGCCCCACACCATCCGCCGCATCCTGGATAGCAGCGATTGCGCCAACCCCGA
CGACTTTCGCACCTGCCGCGAGATCTACCCCGGTCGCGAGGATCCCCGCTCGCAGCGGCTGGTTTTGTCGGAGAGCGTCG
CCCAGCAGATGACCGCCGTATTGCAAGGGGTGATCCGCTCCGGCACCGGCACCGCCGCCCGCCTTGGGATCGGGGAGGCC
GGCAAAACGGGCACCACCACCGCCAACAGGGATCTGCTCTTTGTCGGCTACACGCCCAATCCGCCTCTGGTAACCGGCAT
TTGGCTGGGCAACGACGACGCCAGCCCCACCCGCGGCAGCAGCAGCATCGCCGCCCAAACTTGGAGAAACTACATGAGCC
AAGTCATTCAATAG

Upstream 100 bases:

>100_bases
CCCAGCCCCCAGAGCCACTCCAGCCGCCGCCGGCCCTCGTCCCGGCCAGACCCTGCAGGAGCTGCTGCGCCGCGCGCGCG
AATTGCGGGGAGATTGAGCC

Downstream 100 bases:

>100_bases
GCGACCTTGGCAAACAAGTTCTAACAACTTGACAGAAAACCTCTCCCCCTTCTCCAGGGGGAAGTGCCCGGTGGGAGCGT
TGCAAGCTGTCGCGAAACCG

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 777; Mature: 776

Protein sequence:

>777_residues
MSASEDKNSLRGTVARFFKTLARPFPVGPTALLKPAEPPPPKRPVRLQITCPGEPPQVFVLQGSDILGRSQTVATIRIPA
PAVSHLHARIRPRPGWRLPGLGWRIPLHPLLYWLGWNAGRYQLEDQDSTNGVFRLKPFGGAERVRRVILRHGQRLSLGPP
RDPESVVIQVLDPPPPQVYGVRGCLILMALLGLGARLWIGHEWSKFSVEPPLAADRNPLIVLAGDRQTELRRHQGDLYRE
LPNLEAFGPILPKVVVAAEDHRFYSHFGVDLLGIARAFWVNLRSGEVQQGGSSISQQLARTVLRDYTGSGNTFGRKLREA
VAALKLERRYSKDEILTLYLNNVYLGNGLYGFETAAQFYFGIPSRQLSLSEAATLAAILPAPNAFNPVANYEAAVRGRDR
VLDRLSELKVFPEEEIRRARRSRLTLNPNLRSQTSTVAPYFYSTVFQELRQLLGQDLSAEGNFVIETTLHLPYQRLAEQA
LAQTVREVGSRLGFNQGALVSLDSRNGEILALVGGIDYTASPFNRATQAQRQPGSTFKVFAYAAALSQGIPLSQVLSCEP
LTWGNLTFQGCRSGSAPMDLARGLILSENVIALRLAQQVGLERVVAMARQMGIQSPLQPYPSLVLGTMEVNLLELTAAFA
PLANRGLWSRPHTIRRILDSSDCANPDDFRTCREIYPGREDPRSQRLVLSESVAQQMTAVLQGVIRSGTGTAARLGIGEA
GKTGTTTANRDLLFVGYTPNPPLVTGIWLGNDDASPTRGSSSIAAQTWRNYMSQVIQ

Sequences:

>Translated_777_residues
MSASEDKNSLRGTVARFFKTLARPFPVGPTALLKPAEPPPPKRPVRLQITCPGEPPQVFVLQGSDILGRSQTVATIRIPA
PAVSHLHARIRPRPGWRLPGLGWRIPLHPLLYWLGWNAGRYQLEDQDSTNGVFRLKPFGGAERVRRVILRHGQRLSLGPP
RDPESVVIQVLDPPPPQVYGVRGCLILMALLGLGARLWIGHEWSKFSVEPPLAADRNPLIVLAGDRQTELRRHQGDLYRE
LPNLEAFGPILPKVVVAAEDHRFYSHFGVDLLGIARAFWVNLRSGEVQQGGSSISQQLARTVLRDYTGSGNTFGRKLREA
VAALKLERRYSKDEILTLYLNNVYLGNGLYGFETAAQFYFGIPSRQLSLSEAATLAAILPAPNAFNPVANYEAAVRGRDR
VLDRLSELKVFPEEEIRRARRSRLTLNPNLRSQTSTVAPYFYSTVFQELRQLLGQDLSAEGNFVIETTLHLPYQRLAEQA
LAQTVREVGSRLGFNQGALVSLDSRNGEILALVGGIDYTASPFNRATQAQRQPGSTFKVFAYAAALSQGIPLSQVLSCEP
LTWGNLTFQGCRSGSAPMDLARGLILSENVIALRLAQQVGLERVVAMARQMGIQSPLQPYPSLVLGTMEVNLLELTAAFA
PLANRGLWSRPHTIRRILDSSDCANPDDFRTCREIYPGREDPRSQRLVLSESVAQQMTAVLQGVIRSGTGTAARLGIGEA
GKTGTTTANRDLLFVGYTPNPPLVTGIWLGNDDASPTRGSSSIAAQTWRNYMSQVIQ
>Mature_776_residues
SASEDKNSLRGTVARFFKTLARPFPVGPTALLKPAEPPPPKRPVRLQITCPGEPPQVFVLQGSDILGRSQTVATIRIPAP
AVSHLHARIRPRPGWRLPGLGWRIPLHPLLYWLGWNAGRYQLEDQDSTNGVFRLKPFGGAERVRRVILRHGQRLSLGPPR
DPESVVIQVLDPPPPQVYGVRGCLILMALLGLGARLWIGHEWSKFSVEPPLAADRNPLIVLAGDRQTELRRHQGDLYREL
PNLEAFGPILPKVVVAAEDHRFYSHFGVDLLGIARAFWVNLRSGEVQQGGSSISQQLARTVLRDYTGSGNTFGRKLREAV
AALKLERRYSKDEILTLYLNNVYLGNGLYGFETAAQFYFGIPSRQLSLSEAATLAAILPAPNAFNPVANYEAAVRGRDRV
LDRLSELKVFPEEEIRRARRSRLTLNPNLRSQTSTVAPYFYSTVFQELRQLLGQDLSAEGNFVIETTLHLPYQRLAEQAL
AQTVREVGSRLGFNQGALVSLDSRNGEILALVGGIDYTASPFNRATQAQRQPGSTFKVFAYAAALSQGIPLSQVLSCEPL
TWGNLTFQGCRSGSAPMDLARGLILSENVIALRLAQQVGLERVVAMARQMGIQSPLQPYPSLVLGTMEVNLLELTAAFAP
LANRGLWSRPHTIRRILDSSDCANPDDFRTCREIYPGREDPRSQRLVLSESVAQQMTAVLQGVIRSGTGTAARLGIGEAG
KTGTTTANRDLLFVGYTPNPPLVTGIWLGNDDASPTRGSSSIAAQTWRNYMSQVIQ

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=532, Percent_Identity=30.4511278195489, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI1788867, Length=605, Percent_Identity=29.4214876033058, Blast_Score=170, Evalue=4e-43,
Organism=Escherichia coli, GI87082258, Length=278, Percent_Identity=35.9712230215827, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1789601, Length=196, Percent_Identity=36.2244897959184, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 85538; Mature: 85407

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50006 FHA_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASEDKNSLRGTVARFFKTLARPFPVGPTALLKPAEPPPPKRPVRLQITCPGEPPQVFV
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCEEEE
LQGSDILGRSQTVATIRIPAPAVSHLHARIRPRPGWRLPGLGWRIPLHPLLYWLGWNAGR
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
YQLEDQDSTNGVFRLKPFGGAERVRRVILRHGQRLSLGPPRDPESVVIQVLDPPPPQVYG
EEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCCCHHH
VRGCLILMALLGLGARLWIGHEWSKFSVEPPLAADRNPLIVLAGDRQTELRRHQGDLYRE
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
LPNLEAFGPILPKVVVAAEDHRFYSHFGVDLLGIARAFWVNLRSGEVQQGGSSISQQLAR
CCCCCHHHHHHHHEEEEECCCHHHHHHCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHH
TVLRDYTGSGNTFGRKLREAVAALKLERRYSKDEILTLYLNNVYLGNGLYGFETAAQFYF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHC
GIPSRQLSLSEAATLAAILPAPNAFNPVANYEAAVRGRDRVLDRLSELKVFPEEEIRRAR
CCCCCCCCHHHHHHHHEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
RSRLTLNPNLRSQTSTVAPYFYSTVFQELRQLLGQDLSAEGNFVIETTLHLPYQRLAEQA
HHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHH
LAQTVREVGSRLGFNQGALVSLDSRNGEILALVGGIDYTASPFNRATQAQRQPGSTFKVF
HHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHH
AYAAALSQGIPLSQVLSCEPLTWGNLTFQGCRSGSAPMDLARGLILSENVIALRLAQQVG
HHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCCCCCHHHHHCCHHCCCCHHHHHHHHHHH
LERVVAMARQMGIQSPLQPYPSLVLGTMEVNLLELTAAFAPLANRGLWSRPHTIRRILDS
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC
SDCANPDDFRTCREIYPGREDPRSQRLVLSESVAQQMTAVLQGVIRSGTGTAARLGIGEA
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
GKTGTTTANRDLLFVGYTPNPPLVTGIWLGNDDASPTRGSSSIAAQTWRNYMSQVIQ
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SASEDKNSLRGTVARFFKTLARPFPVGPTALLKPAEPPPPKRPVRLQITCPGEPPQVFV
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCEEEE
LQGSDILGRSQTVATIRIPAPAVSHLHARIRPRPGWRLPGLGWRIPLHPLLYWLGWNAGR
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
YQLEDQDSTNGVFRLKPFGGAERVRRVILRHGQRLSLGPPRDPESVVIQVLDPPPPQVYG
EEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCCCHHH
VRGCLILMALLGLGARLWIGHEWSKFSVEPPLAADRNPLIVLAGDRQTELRRHQGDLYRE
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
LPNLEAFGPILPKVVVAAEDHRFYSHFGVDLLGIARAFWVNLRSGEVQQGGSSISQQLAR
CCCCCHHHHHHHHEEEEECCCHHHHHHCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHH
TVLRDYTGSGNTFGRKLREAVAALKLERRYSKDEILTLYLNNVYLGNGLYGFETAAQFYF
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHHHHHC
GIPSRQLSLSEAATLAAILPAPNAFNPVANYEAAVRGRDRVLDRLSELKVFPEEEIRRAR
CCCCCCCCHHHHHHHHEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHH
RSRLTLNPNLRSQTSTVAPYFYSTVFQELRQLLGQDLSAEGNFVIETTLHLPYQRLAEQA
HHCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHH
LAQTVREVGSRLGFNQGALVSLDSRNGEILALVGGIDYTASPFNRATQAQRQPGSTFKVF
HHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCHHH
AYAAALSQGIPLSQVLSCEPLTWGNLTFQGCRSGSAPMDLARGLILSENVIALRLAQQVG
HHHHHHHCCCCHHHHHCCCCCCCCCEEEECCCCCCCHHHHHCCHHCCCCHHHHHHHHHHH
LERVVAMARQMGIQSPLQPYPSLVLGTMEVNLLELTAAFAPLANRGLWSRPHTIRRILDS
HHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC
SDCANPDDFRTCREIYPGREDPRSQRLVLSESVAQQMTAVLQGVIRSGTGTAARLGIGEA
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
GKTGTTTANRDLLFVGYTPNPPLVTGIWLGNDDASPTRGSSSIAAQTWRNYMSQVIQ
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]