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The map label for this gene is nadE

Identifier: 86604930

GI number: 86604930

Start: 210133

End: 211803

Strand: Reverse

Name: nadE

Synonym: CYA_0206

Alternate gene names: 86604930

Gene position: 211803-210133 (Counterclockwise)

Preceding gene: 86604931

Following gene: 86604928

Centisome position: 7.22

GC content: 64.33

Gene sequence:

>1671_bases
GTGAAGGTAGCGCTGTTGCAACTCAACTATACCGTTGGCGATCTCAGCGGCAATGCCGAGCGCATCCGTGCGGCGGTCGA
GGCGGTGGCAGCGGCAGGAGCGGAGCTGGCCATTACTTCTGAGCTGGCCCTGTTGGGGTACCCGCCCCGGGATCTGTTGC
TCTACCCGGCCCTGATTGAGAGGGTAGGCCAAGTTTTGCAGCAGTTGGCGGCCCGGCTGCGGGAGAGCATTCCGGTGGTG
GTGGGATCCGTGCAGCCCAACCCGCTGCCGGAGGGGCGACCCCTCTACAACGCCGCGGCCTGGCTGGAAGGGGGGCAGAT
CCGCCACTGGTTCCAGAAGTCGCTCCTGCCCACCTACGATGTCTTCGACGAAGACCGCTACTTTGAGCCGGGATCCCCGC
CCCAGCCCATTGAGTACCGTAGCCGCCGAGTGGGCTTCACCATCTGTGAAGACATCTGGAACGACCGCGACTTTTGGCAA
CACCGCCGCTACCACCGGGATCCGGTGGAAGAGATGGCCCGCTGCGGCGCCGATGTGCTGATCAACCTGTCGGCCTCTCC
CTTTAGCCTGGGCAAGCAAAAATTGCGGGTGCAGATGCTGGGATCCCTGGCCCGCAAGCACGCCCTGCCCATCCTCTATG
TCAACCAGGTGGGGGGCAACGACGACCTCATTTTCGACGGGGCCAGTTGCGCCCTAGACTGCCGGGGCCGGGTGGTGGCC
CAAGCCGCTGCCTTTCAGCCGGACTGCCTGATGGTAGATGTGGAGGCGCTGGTTCACCCTCCTGAGGAAGGCCCGGAAGT
TCAGGGATCCCGCTGGCCAGCGGAGCCGGAAGCGGAGCTCTACGAGGCGCTGGTTTTGGGCACCCGCGACTACGTCAGCA
AATGTGGCTTTCGCGAGGTGTTGCTGGGCCTGTCGGGAGGGATTGACTCGGCTGTTACTGCCGTCATTGCCGCCGACGCC
TTGGGGCCAGAGCGGGTGCTGGGGGTGCTCATGCCCTCTCCCTATTCCAGTCCCGGCAGCCTGACGGACGCCTACCAACT
GGCGCAAAACCTGGGGATCCCGACCCTGAAGCTGCCCATCCAGTCGATTATGGAGAGCTACCAGCACCTCCTGGCTGAGC
CCTTCGCTGGCCTGCCCCCCGACATCACGGAAGAGAACCTGCAGTCGCGCATCCGCGGCACCTTGCTCATGGCCCTCTCC
AACAAGTTTGGCCGGCTGCTCTTGACCACGGGCAACAAGTCGGAGCTGGCGGTGGGCTACTGCACCATCTACGGGGATAT
GTCCGGTGGCCTGGCCGTGATTTCCGATGTGCCCAAAACCTGGGTCTACCGCCTGGCCCGCTGGATCAACCGCCACCAGG
AACGGATTCCCCTCCCCATCCTCACCAAGCCCCCCTCAGCAGAGCTGCGACCTGGCCAGAAAGATAGCGATAGCCTGCCT
CCCTACGACTTGCTGGACGCTATCCTCCAGCGGCACATCGAACAGCACCAGTCGGCCGGTGAGCTGGTGGCCCAGGGGTT
TGAACCTGACACGGTGCAGCAGGTGCTGCACCTGGTGCAGAGTGCCGAGTTCAAGCGCCACCAGGCTCCCCCTGGCCTGC
GCGTGACCGATCGCGCCTTTGGCACCGGCTGGCGCATGCCCATTGCCAAACGCCAGGAAGGGATCAGTTGA

Upstream 100 bases:

>100_bases
TGCCAGCAACAGCGGAGTAGCCGACCCGCCTGTGGGCAAGCCGAACGACCTGAGCTGGCCTATCATCGTGGAGTCCGCCA
TTCTCGGCCAAGGGGGCCAA

Downstream 100 bases:

>100_bases
CCTTCACCCCCGGTCCCTCTCCCAGAGGGAGAGGAGGGTTGGGAGAGCGCAACCGGTTTCGCTTTTCCTGCAAGGATTGG
GGTAGGGGTGCGGGTATGTT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKVALLQLNYTVGDLSGNAERIRAAVEAVAAAGAELAITSELALLGYPPRDLLLYPALIERVGQVLQQLAARLRESIPVV
VGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYDVFDEDRYFEPGSPPQPIEYRSRRVGFTICEDIWNDRDFWQ
HRRYHRDPVEEMARCGADVLINLSASPFSLGKQKLRVQMLGSLARKHALPILYVNQVGGNDDLIFDGASCALDCRGRVVA
QAAAFQPDCLMVDVEALVHPPEEGPEVQGSRWPAEPEAELYEALVLGTRDYVSKCGFREVLLGLSGGIDSAVTAVIAADA
LGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPIQSIMESYQHLLAEPFAGLPPDITEENLQSRIRGTLLMALS
NKFGRLLLTTGNKSELAVGYCTIYGDMSGGLAVISDVPKTWVYRLARWINRHQERIPLPILTKPPSAELRPGQKDSDSLP
PYDLLDAILQRHIEQHQSAGELVAQGFEPDTVQQVLHLVQSAEFKRHQAPPGLRVTDRAFGTGWRMPIAKRQEGIS

Sequences:

>Translated_556_residues
MKVALLQLNYTVGDLSGNAERIRAAVEAVAAAGAELAITSELALLGYPPRDLLLYPALIERVGQVLQQLAARLRESIPVV
VGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYDVFDEDRYFEPGSPPQPIEYRSRRVGFTICEDIWNDRDFWQ
HRRYHRDPVEEMARCGADVLINLSASPFSLGKQKLRVQMLGSLARKHALPILYVNQVGGNDDLIFDGASCALDCRGRVVA
QAAAFQPDCLMVDVEALVHPPEEGPEVQGSRWPAEPEAELYEALVLGTRDYVSKCGFREVLLGLSGGIDSAVTAVIAADA
LGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPIQSIMESYQHLLAEPFAGLPPDITEENLQSRIRGTLLMALS
NKFGRLLLTTGNKSELAVGYCTIYGDMSGGLAVISDVPKTWVYRLARWINRHQERIPLPILTKPPSAELRPGQKDSDSLP
PYDLLDAILQRHIEQHQSAGELVAQGFEPDTVQQVLHLVQSAEFKRHQAPPGLRVTDRAFGTGWRMPIAKRQEGIS
>Mature_556_residues
MKVALLQLNYTVGDLSGNAERIRAAVEAVAAAGAELAITSELALLGYPPRDLLLYPALIERVGQVLQQLAARLRESIPVV
VGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYDVFDEDRYFEPGSPPQPIEYRSRRVGFTICEDIWNDRDFWQ
HRRYHRDPVEEMARCGADVLINLSASPFSLGKQKLRVQMLGSLARKHALPILYVNQVGGNDDLIFDGASCALDCRGRVVA
QAAAFQPDCLMVDVEALVHPPEEGPEVQGSRWPAEPEAELYEALVLGTRDYVSKCGFREVLLGLSGGIDSAVTAVIAADA
LGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPIQSIMESYQHLLAEPFAGLPPDITEENLQSRIRGTLLMALS
NKFGRLLLTTGNKSELAVGYCTIYGDMSGGLAVISDVPKTWVYRLARWINRHQERIPLPILTKPPSAELRPGQKDSDSLP
PYDLLDAILQRHIEQHQSAGELVAQGFEPDTVQQVLHLVQSAEFKRHQAPPGLRVTDRAFGTGWRMPIAKRQEGIS

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61234; Mature: 61234

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVALLQLNYTVGDLSGNAERIRAAVEAVAAAGAELAITSELALLGYPPRDLLLYPALIE
CEEEEEEEECEEECCCCCHHHHHHHHHHHHHCCCCEEEECCHHEECCCCHHHHHHHHHHH
RVGQVLQQLAARLRESIPVVVGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYD
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCC
VFDEDRYFEPGSPPQPIEYRSRRVGFTICEDIWNDRDFWQHRRYHRDPVEEMARCGADVL
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCEE
INLSASPFSLGKQKLRVQMLGSLARKHALPILYVNQVGGNDDLIFDGASCALDCRGRVVA
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCEEECCCCHHHH
QAAAFQPDCLMVDVEALVHPPEEGPEVQGSRWPAEPEAELYEALVLGTRDYVSKCGFREV
HHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHHHH
LLGLSGGIDSAVTAVIAADALGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPI
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHH
QSIMESYQHLLAEPFAGLPPDITEENLQSRIRGTLLMALSNKFGRLLLTTGNKSELAVGY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEE
CTIYGDMSGGLAVISDVPKTWVYRLARWINRHQERIPLPILTKPPSAELRPGQKDSDSLP
EEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
PYDLLDAILQRHIEQHQSAGELVAQGFEPDTVQQVLHLVQSAEFKRHQAPPGLRVTDRAF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
GTGWRMPIAKRQEGIS
CCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKVALLQLNYTVGDLSGNAERIRAAVEAVAAAGAELAITSELALLGYPPRDLLLYPALIE
CEEEEEEEECEEECCCCCHHHHHHHHHHHHHCCCCEEEECCHHEECCCCHHHHHHHHHHH
RVGQVLQQLAARLRESIPVVVGSVQPNPLPEGRPLYNAAAWLEGGQIRHWFQKSLLPTYD
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCC
VFDEDRYFEPGSPPQPIEYRSRRVGFTICEDIWNDRDFWQHRRYHRDPVEEMARCGADVL
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHCCCCEE
INLSASPFSLGKQKLRVQMLGSLARKHALPILYVNQVGGNDDLIFDGASCALDCRGRVVA
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCEEECCCCHHHH
QAAAFQPDCLMVDVEALVHPPEEGPEVQGSRWPAEPEAELYEALVLGTRDYVSKCGFREV
HHHCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCHHHH
LLGLSGGIDSAVTAVIAADALGPERVLGVLMPSPYSSPGSLTDAYQLAQNLGIPTLKLPI
HHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEHHHH
QSIMESYQHLLAEPFAGLPPDITEENLQSRIRGTLLMALSNKFGRLLLTTGNKSELAVGY
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEE
CTIYGDMSGGLAVISDVPKTWVYRLARWINRHQERIPLPILTKPPSAELRPGQKDSDSLP
EEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCC
PYDLLDAILQRHIEQHQSAGELVAQGFEPDTVQQVLHLVQSAEFKRHQAPPGLRVTDRAF
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
GTGWRMPIAKRQEGIS
CCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]