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The map label for this gene is clpB

Identifier: 86604906

GI number: 86604906

Start: 176758

End: 179400

Strand: Reverse

Name: clpB

Synonym: CYA_0180

Alternate gene names: 86604906

Gene position: 179400-176758 (Counterclockwise)

Preceding gene: 86604907

Following gene: 86604902

Centisome position: 6.12

GC content: 62.28

Gene sequence:

>2643_bases
ATGCAGCCCACCAATCCCAACCAATTTACCGAGAAAGCCTGGAGCGCCATTGTCCAATCCCAAGATGTAGCCCGGCGGCA
CCAGCAGCAACAGTTGGAAACCGAGCATTTGCTGATCTCCCTTCTGGATCAAGAGGGGGGTCTCACCCAGACCATTTTGC
AGCGGGCCGGCCTCGATCCGAGGCTGGTGCGGGATAGAGTGGAAAGCTTTATCAACCAGCAGCCCAAGCTGGCGCGGGTG
GATCAGCTCTACCTAGGCCGCGGCCTGGAGACCACGCTGGATCGAGCCGAGGAGTTTCGCAAAGAGTATGGCGATGATTT
TATCTCGGTAGAGCACTTGCTTTTGGGATCCCTGGACGACGAGCGGGTGGGCAAGCGCGTTCTAGCACCCCTGGGCTTGA
CGGTCGAGCGCCTCAAGCCCGTTATCCAACAGGTGCGAGGCAAACAGCGGGTTACCGACAAAAATCCCGAGTCCCGCTAC
GAGGCCCTGGAGCGCTATGGCCGCGACTTGACCAAGGCCGCCCGCGAAGGGAAGCTGGATCCGGTGATTGGGCGGGACGA
CGAGATCCGCCGCGTCATTCAGGTGCTCTCCCGCCGCACCAAAAACAACCCCGTCCTCATCGGCGAGCCGGGTGTGGGGA
AAACCGCCATCGTCGAGGGCCTGGCCCAGCGCATCAACCGGGGAGACGTGCCGGAGTCGCTGAAAAACCGGCGGGTGATC
GCCCTGGATATGGGATCCCTGATTGCCGGCGCCAAGTACCGCGGCGAGTTTGAAGACCGCCTCAAAGCCGTGCTCAAGGA
GGTCACCGAATCGGAAGGGCAGATCATCCTCTTCATCGACGAGCTGCACACCGTGGTGGGGGCAGGGGCGGCCGAAGGGG
CGATGGATGCCGGCAACCTGCTCAAGCCCATGCTGGCCCGCGGCGAATTGCGCTGCATTGGCGCCACCACCCTGGATGAA
TACCGCAAGCACATCGAAAAAGACGCCGCCCTGGAGCGCCGCTTCCAGCCGGTGTATGTGGATCAGCCCTCCGTGGAAGA
CACCATCTCCATCCTGCGCGGCCTCAAAGAGCGCTACGAGCTGCACCACGGGGTCAAAATCAGCGACTCGGCCCTGGTGG
CAGCGGCAGTCCTCTCCAACCGCTACATCTCCGAGCGCTTCCTGCCCGACAAGGCCATCGACCTGGTGGACGAAGCGGCG
GCCAAGCTGAAGATGGAGATCACCTCCAAGCCCGTCGAATTGGATGAAATCGACCGGCGCATCATGCAACTGCAGATGGA
GGAGCTCTCCCTCAAGAAAGAAGACGACCCCGCCTCCAAAGAGCGCCTGGGCAAAATTGAGCAGGAGCTAGCCAACTTGA
TGGAGCGGCAGCGGGAGCTGAGCGCCCGTTGGCAAATGGAAAAAGAGGCCATCGAGCGGCTGCAAAACCTCAAGGCCGAG
CGGGATGCCGTCAAGCTGCAGATTGAACAGGCAGAACAAAAATATGACCTCAACCGCGCTGCCGAGCTCAAATACGGCAA
GCTCATGGAGCTGGAGCGCCAGATCCAAGAGCAGGAGGCCCACCTGGCCAAGCTGCAGGCCAACGGCGAGCCCTTGTTGC
GGGAGCAGGTGACGCCCGAAGACATTGCCGAGATCGTCTCCCGCTGGACCGGGATCCCGGTCACCAGCCTGATGGAGTCG
GAAAAGCAGAAGCTGCTGCGCCTGGAAGCCCACTTGCACCAGCGGGTGGTGGGCCAGGACGAGGCGGTAGCGGCGGTGGC
AGCCGCCATCCGGCGGGCCCGGGCCGGCCTCAAGGATCCCAATCGGCCCATCGGCTCGTTCCTGTTCATGGGGCCCACCG
GCGTCGGGAAGACCGAGCTGGCCCGCGCCCTGGCGGAGTTCCTCTTCGACACCTCCGAGGCCATGGTGCGCATCGACATG
TCGGAGTACATGGAGAAGCACTCCGTCTCCCGCTTGATTGGGGCTCCGCCGGGCTATGTGGGCTACGAAGAAGGCGGGCA
GCTCTCGGAAGCGGTGCGGCGGCGCCCCTACTCGGTGGTGCTCTTTGACGAGGTGGAAAAAGCCCACCCTGATGTCTTCA
ACATCCTGCTGCAGGTGCTGGACGACGGCCGCATCACCGACTCCCAAGGCAGGACGGTGGACTTCAAAAACACCATCATC
ATCATGACCAGCAACATTGGCAGCGAGCTCATCCTGGAAATTGGCGGCGACGAGAGCCGCTACGAGGAGATGAAAAGCCA
GGTGCTGGCCATGCTGCGGCACCACTTCCGGCCGGAGTTCCTCAACCGGGTGGATGAACTGATCATCTTCCACGCTCTAA
CCAAAGAGCAAATCCGCCAGATCGTCAGCCTGCAGATGCGGCGGGTGGAGCAGTTGCTGGCGGATCAGCAAATTGAAATT
GTCCTCACCGAAGAGGCCAAGGACTACCTGGCCGAGCTGGGCTACGACCCCGTGTTTGGCGCCCGCCCGCTGAAGCGGGT
AATCCAGCGGGAGATCGAAAACCCCCTCGCCACCAAGCTGCTGGAAAACGAGTTCCTGCCCGGCGACCGCATCCTGGTGG
AGGTGGCCAACGAGCGGCTGATCTTCCGCAAGATCGGCTCTCAGCCCCGCCAAGAGCCGCCTGCTGCTGTAGCCGTCAGC
TAG

Upstream 100 bases:

>100_bases
CAGCAGCGCTTCCCCAAGTCGGCAGCAAGGGCTGAGAGCCCCTTGGCTGCCCCCTGCATTGTCTTCCTATACACTGTAAA
AAAACTCCTGCCAAACCCCT

Downstream 100 bases:

>100_bases
GCCAGTTGGCTAGAGGCTGATCACCTGGTCAGGTGGGTTGTCTTGCAAGGCCGCGTAGAGGTCGGGGAAGCAGCCCACCT
GCACCCCTTTGACCACGTTT

Product: ATP-dependent chaperone protein ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MQPTNPNQFTEKAWSAIVQSQDVARRHQQQQLETEHLLISLLDQEGGLTQTILQRAGLDPRLVRDRVESFINQQPKLARV
DQLYLGRGLETTLDRAEEFRKEYGDDFISVEHLLLGSLDDERVGKRVLAPLGLTVERLKPVIQQVRGKQRVTDKNPESRY
EALERYGRDLTKAAREGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRINRGDVPESLKNRRVI
ALDMGSLIAGAKYRGEFEDRLKAVLKEVTESEGQIILFIDELHTVVGAGAAEGAMDAGNLLKPMLARGELRCIGATTLDE
YRKHIEKDAALERRFQPVYVDQPSVEDTISILRGLKERYELHHGVKISDSALVAAAVLSNRYISERFLPDKAIDLVDEAA
AKLKMEITSKPVELDEIDRRIMQLQMEELSLKKEDDPASKERLGKIEQELANLMERQRELSARWQMEKEAIERLQNLKAE
RDAVKLQIEQAEQKYDLNRAAELKYGKLMELERQIQEQEAHLAKLQANGEPLLREQVTPEDIAEIVSRWTGIPVTSLMES
EKQKLLRLEAHLHQRVVGQDEAVAAVAAAIRRARAGLKDPNRPIGSFLFMGPTGVGKTELARALAEFLFDTSEAMVRIDM
SEYMEKHSVSRLIGAPPGYVGYEEGGQLSEAVRRRPYSVVLFDEVEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTII
IMTSNIGSELILEIGGDESRYEEMKSQVLAMLRHHFRPEFLNRVDELIIFHALTKEQIRQIVSLQMRRVEQLLADQQIEI
VLTEEAKDYLAELGYDPVFGARPLKRVIQREIENPLATKLLENEFLPGDRILVEVANERLIFRKIGSQPRQEPPAAVAVS

Sequences:

>Translated_880_residues
MQPTNPNQFTEKAWSAIVQSQDVARRHQQQQLETEHLLISLLDQEGGLTQTILQRAGLDPRLVRDRVESFINQQPKLARV
DQLYLGRGLETTLDRAEEFRKEYGDDFISVEHLLLGSLDDERVGKRVLAPLGLTVERLKPVIQQVRGKQRVTDKNPESRY
EALERYGRDLTKAAREGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRINRGDVPESLKNRRVI
ALDMGSLIAGAKYRGEFEDRLKAVLKEVTESEGQIILFIDELHTVVGAGAAEGAMDAGNLLKPMLARGELRCIGATTLDE
YRKHIEKDAALERRFQPVYVDQPSVEDTISILRGLKERYELHHGVKISDSALVAAAVLSNRYISERFLPDKAIDLVDEAA
AKLKMEITSKPVELDEIDRRIMQLQMEELSLKKEDDPASKERLGKIEQELANLMERQRELSARWQMEKEAIERLQNLKAE
RDAVKLQIEQAEQKYDLNRAAELKYGKLMELERQIQEQEAHLAKLQANGEPLLREQVTPEDIAEIVSRWTGIPVTSLMES
EKQKLLRLEAHLHQRVVGQDEAVAAVAAAIRRARAGLKDPNRPIGSFLFMGPTGVGKTELARALAEFLFDTSEAMVRIDM
SEYMEKHSVSRLIGAPPGYVGYEEGGQLSEAVRRRPYSVVLFDEVEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTII
IMTSNIGSELILEIGGDESRYEEMKSQVLAMLRHHFRPEFLNRVDELIIFHALTKEQIRQIVSLQMRRVEQLLADQQIEI
VLTEEAKDYLAELGYDPVFGARPLKRVIQREIENPLATKLLENEFLPGDRILVEVANERLIFRKIGSQPRQEPPAAVAVS
>Mature_880_residues
MQPTNPNQFTEKAWSAIVQSQDVARRHQQQQLETEHLLISLLDQEGGLTQTILQRAGLDPRLVRDRVESFINQQPKLARV
DQLYLGRGLETTLDRAEEFRKEYGDDFISVEHLLLGSLDDERVGKRVLAPLGLTVERLKPVIQQVRGKQRVTDKNPESRY
EALERYGRDLTKAAREGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRINRGDVPESLKNRRVI
ALDMGSLIAGAKYRGEFEDRLKAVLKEVTESEGQIILFIDELHTVVGAGAAEGAMDAGNLLKPMLARGELRCIGATTLDE
YRKHIEKDAALERRFQPVYVDQPSVEDTISILRGLKERYELHHGVKISDSALVAAAVLSNRYISERFLPDKAIDLVDEAA
AKLKMEITSKPVELDEIDRRIMQLQMEELSLKKEDDPASKERLGKIEQELANLMERQRELSARWQMEKEAIERLQNLKAE
RDAVKLQIEQAEQKYDLNRAAELKYGKLMELERQIQEQEAHLAKLQANGEPLLREQVTPEDIAEIVSRWTGIPVTSLMES
EKQKLLRLEAHLHQRVVGQDEAVAAVAAAIRRARAGLKDPNRPIGSFLFMGPTGVGKTELARALAEFLFDTSEAMVRIDM
SEYMEKHSVSRLIGAPPGYVGYEEGGQLSEAVRRRPYSVVLFDEVEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTII
IMTSNIGSELILEIGGDESRYEEMKSQVLAMLRHHFRPEFLNRVDELIIFHALTKEQIRQIVSLQMRRVEQLLADQQIEI
VLTEEAKDYLAELGYDPVFGARPLKRVIQREIENPLATKLLENEFLPGDRILVEVANERLIFRKIGSQPRQEPPAAVAVS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=329, Percent_Identity=37.9939209726444, Blast_Score=222, Evalue=1e-57,
Organism=Escherichia coli, GI1788943, Length=856, Percent_Identity=57.9439252336449, Blast_Score=993, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=406, Percent_Identity=41.1330049261084, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=712, Percent_Identity=54.2134831460674, Blast_Score=790, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=855, Percent_Identity=43.5087719298246, Blast_Score=690, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 99752; Mature: 99752

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPTNPNQFTEKAWSAIVQSQDVARRHQQQQLETEHLLISLLDQEGGLTQTILQRAGLDP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCH
RLVRDRVESFINQQPKLARVDQLYLGRGLETTLDRAEEFRKEYGDDFISVEHLLLGSLDD
HHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCH
ERVGKRVLAPLGLTVERLKPVIQQVRGKQRVTDKNPESRYEALERYGRDLTKAAREGKLD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRINRGDVPESLKNRRVI
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCEEE
ALDMGSLIAGAKYRGEFEDRLKAVLKEVTESEGQIILFIDELHTVVGAGAAEGAMDAGNL
EEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPMLARGELRCIGATTLDEYRKHIEKDAALERRFQPVYVDQPSVEDTISILRGLKERYE
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHH
LHHGVKISDSALVAAAVLSNRYISERFLPDKAIDLVDEAAAKLKMEITSKPVELDEIDRR
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IMQLQMEELSLKKEDDPASKERLGKIEQELANLMERQRELSARWQMEKEAIERLQNLKAE
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAVKLQIEQAEQKYDLNRAAELKYGKLMELERQIQEQEAHLAKLQANGEPLLREQVTPE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
DIAEIVSRWTGIPVTSLMESEKQKLLRLEAHLHQRVVGQDEAVAAVAAAIRRARAGLKDP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NRPIGSFLFMGPTGVGKTELARALAEFLFDTSEAMVRIDMSEYMEKHSVSRLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGQLSEAVRRRPYSVVLFDEVEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTII
CCCCCCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEE
IMTSNIGSELILEIGGDESRYEEMKSQVLAMLRHHFRPEFLNRVDELIIFHALTKEQIRQ
EEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IVSLQMRRVEQLLADQQIEIVLTEEAKDYLAELGYDPVFGARPLKRVIQREIENPLATKL
HHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHH
LENEFLPGDRILVEVANERLIFRKIGSQPRQEPPAAVAVS
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC
>Mature Secondary Structure
MQPTNPNQFTEKAWSAIVQSQDVARRHQQQQLETEHLLISLLDQEGGLTQTILQRAGLDP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCH
RLVRDRVESFINQQPKLARVDQLYLGRGLETTLDRAEEFRKEYGDDFISVEHLLLGSLDD
HHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCH
ERVGKRVLAPLGLTVERLKPVIQQVRGKQRVTDKNPESRYEALERYGRDLTKAAREGKLD
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRINRGDVPESLKNRRVI
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCEEE
ALDMGSLIAGAKYRGEFEDRLKAVLKEVTESEGQIILFIDELHTVVGAGAAEGAMDAGNL
EEEHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHH
LKPMLARGELRCIGATTLDEYRKHIEKDAALERRFQPVYVDQPSVEDTISILRGLKERYE
HHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHH
LHHGVKISDSALVAAAVLSNRYISERFLPDKAIDLVDEAAAKLKMEITSKPVELDEIDRR
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IMQLQMEELSLKKEDDPASKERLGKIEQELANLMERQRELSARWQMEKEAIERLQNLKAE
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAVKLQIEQAEQKYDLNRAAELKYGKLMELERQIQEQEAHLAKLQANGEPLLREQVTPE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHH
DIAEIVSRWTGIPVTSLMESEKQKLLRLEAHLHQRVVGQDEAVAAVAAAIRRARAGLKDP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NRPIGSFLFMGPTGVGKTELARALAEFLFDTSEAMVRIDMSEYMEKHSVSRLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGQLSEAVRRRPYSVVLFDEVEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTII
CCCCCCHHHHHHHHCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEE
IMTSNIGSELILEIGGDESRYEEMKSQVLAMLRHHFRPEFLNRVDELIIFHALTKEQIRQ
EEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IVSLQMRRVEQLLADQQIEIVLTEEAKDYLAELGYDPVFGARPLKRVIQREIENPLATKL
HHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHH
LENEFLPGDRILVEVANERLIFRKIGSQPRQEPPAAVAVS
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]