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The map label for this gene is metE-2

Identifier: 86604896

GI number: 86604896

Start: 164140

End: 166383

Strand: Reverse

Name: metE-2

Synonym: CYA_0167

Alternate gene names: 86604896

Gene position: 166383-164140 (Counterclockwise)

Preceding gene: 86604897

Following gene: 86604895

Centisome position: 5.67

GC content: 62.03

Gene sequence:

>2244_bases
ATGGCCATTGAAACTCAAACCCTAGGCTATGCCCGCATGGGCAAGCGCCGCGAGCTGAAGAAAGCCCTGGAGGCCTTTTG
GGGCGGCACCCTGGAGGCGGAAGCCTTGTTGGCGACGCTGCAGGATCTGGAGAGTCAGGCCTGGCGAACCCAGCGACAGG
CCAACCTCGACCACATTGCCGTAGGAGATCAAACCCTCTACGACCACGTTTTGGACTGGGCCACCTGGCTGGGGCTGATT
CCCTCCCGGTTTCAGAGCTTATCAGGCCTGGAGCGCTACTTTGCCATGGCGCGGGGGCAGGAGGGGCTGCCGGCCTTGGA
AATGACCAAGTGGTTTGACACCAACTACCACTACCTGGTGCCGGAAATTGAGGCCGACGCGATCCCTCAGGCCCATTTCG
ACGACTTCCTGGCCACGGTGCGACGGGCGCAAGGGATCCTAGGAAACCGCACCAGCCCCGTCCTCCTCAGTCCGGTTACC
CTTCTTGCTCTAAGCCAGCGCTCTGGCGAGTTGCGGGGGGATCTGGAGAAGCTGCTTCCTCTCTACAGAGAACTCTTGCA
GGAGCTGCAGCGCCTGGGGATCCGGGAAGTGCAAGTCCATGAGCCCATCTTGGTGACCAGCGAAGGCAAAAGTCTTCAGG
AAGCGGTGGAGCAGACCTACCGCTGGCTTGCAAGCGTGGGGATCCCTCTCCACCTGGTTACCTACTTTGACGACTTGGGG
GAGACTTACCCCTGGGCGGTGGAGCTTCCGGTAGCTGGCCTCAGCCTCGACTTCACCCGCGGCCACAACCTGGAACTGGT
CAGAGCCCACGGCTTCCCCGCCGACAAGATCCTGGGGGCGGGGGTAGTGGACGGGCGCAACATCTGGCAGATCCAGCCCA
AAGCCGTTCTGGCCACTCTGCAAGAGCTGCAAGCCCTAGCCCCCAACCTGCGGGTGCAGCCTTCTTGCTCGCTCCAATTT
GTGCCCCACGACGCTGCTCTGGAAACCCACCTGCCAGAGCCTTTGCGCAATGTTCTCAGCTTTGCCGAGCAGAAGCTGGC
CGAGGTGGTTCTGCTGGCCCGCACCCTCAACGGCGAAGACACCGCCGCCCAGCAGCAGGAGCTGGAGCAGCAGTGGCAGG
CCTTCCAGGACTTCAATCCCCCCAACTCTACAGTGCGCCAGGCCTTGGCTAACCTGACGGCGCAGGACTTCCAGCGCTCC
CTTCCCTACGAGCAGCGTATAGGCCGGCAGGTCAAGCTGCCACCGCTGCCGACCACCACCATCGGCTCTTTTCCCCAAAC
CCCCGAAGTCCGGCAGTGGCGGGTCAAGTACAAAAAGGGCGAGATCTCCCAGGCCGAATACGAAGCTGCCATCGACGCCG
AAATTGCCAAGTGCATCCGCATCCAAGAAGAAATTGGCCTGGATGTGCTGGTGCATGGTGAGTTCGAGCGCACCGACATG
GTGGAATACTTCGCCCAACAACTGCAGGGCTTTGCTTTTACCGAGCACGGCTGGGTGCAGAGCTATGGCAGCCGCTGCGT
GCGTCCCCCCATTCTCTACGGCGACGTGTCTCGCCCTCGCCCCATGACGGTGCGGGAGTTCAAGGTGGCCCAATCTCTGA
CTCAAAAGCCGGTCAAGGGGATGCTGACAGGGCCGGTAACCATGCTCAACTGGTCCTTCCCCCGCGTTGACGTTTCCCGT
CGCGAGCAGGCCCTGCAAATTGCCCTGGCCCTGCGGGCAGAGGTGGCCGACTTGGAAGCTGCCGGAGCCGCGATGGTGCA
GGTGGACGAGCCAGCGCTGCGAGAGGGGCTGCCCTTGAAAAAAGAGCGCTGGCCAGAATACTTAAGTTGGGCGGTGGAGG
CCTTCCGCCTGACTACCGCTGGCGCCAAGCCCGAAACCCAGATCCACACCCACATGTGCTACTCGGAGTTCGGGGACATC
ATCGAGCACATCGAGCGTCTGGATGCCGACGTCCTCTCCATCGAAAACAGCCGCAGCAGCAACAAAACCTTGCTGCAGAT
TGCCCAAGCCGGCTATCGCCACCAGGTAGGCAACGGCGTTTACGACGTTCACAGCCCGGCGGTGCCCGACACCGAGCAGA
TTCTCCGGCAACTGCGCCTGGGAGTGGCCCACTTGCCGCTAGAGCAAACCTGGGTCAACCCCGACTGCGGCCTAAAAACC
CGCCGCTGGGAGGAAGTGATCCCTGCCCTGAAAAACATGGTGGCAGCCACTCAGCGTCTAAGGGAGGAGCTCCAGGCCAC
CTAG

Upstream 100 bases:

>100_bases
GCCACCGATAGGCATGTCGGCGGCCATCTCTTGCTGCCTAGGTTGGATGACAACTGGCGGCAACCTCAATTACCGAATTC
GATTCAGGACAAGGTACCTT

Downstream 100 bases:

>100_bases
CCCCCGGCAAGGTTGCTCAGGCCAGCGTCTCCTCCGCGGGCAACCGTGCGGCTACGACACTCCTAGGCCGCCTTGCAGCT
GGCAAATAGGGCAAGATGAG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MAIETQTLGYARMGKRRELKKALEAFWGGTLEAEALLATLQDLESQAWRTQRQANLDHIAVGDQTLYDHVLDWATWLGLI
PSRFQSLSGLERYFAMARGQEGLPALEMTKWFDTNYHYLVPEIEADAIPQAHFDDFLATVRRAQGILGNRTSPVLLSPVT
LLALSQRSGELRGDLEKLLPLYRELLQELQRLGIREVQVHEPILVTSEGKSLQEAVEQTYRWLASVGIPLHLVTYFDDLG
ETYPWAVELPVAGLSLDFTRGHNLELVRAHGFPADKILGAGVVDGRNIWQIQPKAVLATLQELQALAPNLRVQPSCSLQF
VPHDAALETHLPEPLRNVLSFAEQKLAEVVLLARTLNGEDTAAQQQELEQQWQAFQDFNPPNSTVRQALANLTAQDFQRS
LPYEQRIGRQVKLPPLPTTTIGSFPQTPEVRQWRVKYKKGEISQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
VEYFAQQLQGFAFTEHGWVQSYGSRCVRPPILYGDVSRPRPMTVREFKVAQSLTQKPVKGMLTGPVTMLNWSFPRVDVSR
REQALQIALALRAEVADLEAAGAAMVQVDEPALREGLPLKKERWPEYLSWAVEAFRLTTAGAKPETQIHTHMCYSEFGDI
IEHIERLDADVLSIENSRSSNKTLLQIAQAGYRHQVGNGVYDVHSPAVPDTEQILRQLRLGVAHLPLEQTWVNPDCGLKT
RRWEEVIPALKNMVAATQRLREELQAT

Sequences:

>Translated_747_residues
MAIETQTLGYARMGKRRELKKALEAFWGGTLEAEALLATLQDLESQAWRTQRQANLDHIAVGDQTLYDHVLDWATWLGLI
PSRFQSLSGLERYFAMARGQEGLPALEMTKWFDTNYHYLVPEIEADAIPQAHFDDFLATVRRAQGILGNRTSPVLLSPVT
LLALSQRSGELRGDLEKLLPLYRELLQELQRLGIREVQVHEPILVTSEGKSLQEAVEQTYRWLASVGIPLHLVTYFDDLG
ETYPWAVELPVAGLSLDFTRGHNLELVRAHGFPADKILGAGVVDGRNIWQIQPKAVLATLQELQALAPNLRVQPSCSLQF
VPHDAALETHLPEPLRNVLSFAEQKLAEVVLLARTLNGEDTAAQQQELEQQWQAFQDFNPPNSTVRQALANLTAQDFQRS
LPYEQRIGRQVKLPPLPTTTIGSFPQTPEVRQWRVKYKKGEISQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
VEYFAQQLQGFAFTEHGWVQSYGSRCVRPPILYGDVSRPRPMTVREFKVAQSLTQKPVKGMLTGPVTMLNWSFPRVDVSR
REQALQIALALRAEVADLEAAGAAMVQVDEPALREGLPLKKERWPEYLSWAVEAFRLTTAGAKPETQIHTHMCYSEFGDI
IEHIERLDADVLSIENSRSSNKTLLQIAQAGYRHQVGNGVYDVHSPAVPDTEQILRQLRLGVAHLPLEQTWVNPDCGLKT
RRWEEVIPALKNMVAATQRLREELQAT
>Mature_746_residues
AIETQTLGYARMGKRRELKKALEAFWGGTLEAEALLATLQDLESQAWRTQRQANLDHIAVGDQTLYDHVLDWATWLGLIP
SRFQSLSGLERYFAMARGQEGLPALEMTKWFDTNYHYLVPEIEADAIPQAHFDDFLATVRRAQGILGNRTSPVLLSPVTL
LALSQRSGELRGDLEKLLPLYRELLQELQRLGIREVQVHEPILVTSEGKSLQEAVEQTYRWLASVGIPLHLVTYFDDLGE
TYPWAVELPVAGLSLDFTRGHNLELVRAHGFPADKILGAGVVDGRNIWQIQPKAVLATLQELQALAPNLRVQPSCSLQFV
PHDAALETHLPEPLRNVLSFAEQKLAEVVLLARTLNGEDTAAQQQELEQQWQAFQDFNPPNSTVRQALANLTAQDFQRSL
PYEQRIGRQVKLPPLPTTTIGSFPQTPEVRQWRVKYKKGEISQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDMV
EYFAQQLQGFAFTEHGWVQSYGSRCVRPPILYGDVSRPRPMTVREFKVAQSLTQKPVKGMLTGPVTMLNWSFPRVDVSRR
EQALQIALALRAEVADLEAAGAAMVQVDEPALREGLPLKKERWPEYLSWAVEAFRLTTAGAKPETQIHTHMCYSEFGDII
EHIERLDADVLSIENSRSSNKTLLQIAQAGYRHQVGNGVYDVHSPAVPDTEQILRQLRLGVAHLPLEQTWVNPDCGLKTR
RWEEVIPALKNMVAATQRLREELQAT

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=758, Percent_Identity=47.2295514511873, Blast_Score=632, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=779, Percent_Identity=43.3889602053915, Blast_Score=580, Evalue=1e-166,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84253; Mature: 84122

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIETQTLGYARMGKRRELKKALEAFWGGTLEAEALLATLQDLESQAWRTQRQANLDHIA
CCCCCCCCCHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VGDQTLYDHVLDWATWLGLIPSRFQSLSGLERYFAMARGQEGLPALEMTKWFDTNYHYLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEC
PEIEADAIPQAHFDDFLATVRRAQGILGNRTSPVLLSPVTLLALSQRSGELRGDLEKLLP
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHCCCCHHHHHHHHHH
LYRELLQELQRLGIREVQVHEPILVTSEGKSLQEAVEQTYRWLASVGIPLHLVTYFDDLG
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
ETYPWAVELPVAGLSLDFTRGHNLELVRAHGFPADKILGAGVVDGRNIWQIQPKAVLATL
CCCCEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHCCCCCCCCCEEEECHHHHHHHH
QELQALAPNLRVQPSCSLQFVPHDAALETHLPEPLRNVLSFAEQKLAEVVLLARTLNGED
HHHHHHCCCCEECCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TAAQQQELEQQWQAFQDFNPPNSTVRQALANLTAQDFQRSLPYEQRIGRQVKLPPLPTTT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCC
IGSFPQTPEVRQWRVKYKKGEISQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
VEYFAQQLQGFAFTEHGWVQSYGSRCVRPPILYGDVSRPRPMTVREFKVAQSLTQKPVKG
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
MLTGPVTMLNWSFPRVDVSRREQALQIALALRAEVADLEAAGAAMVQVDEPALREGLPLK
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCC
KERWPEYLSWAVEAFRLTTAGAKPETQIHTHMCYSEFGDIIEHIERLDADVLSIENSRSS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
NKTLLQIAQAGYRHQVGNGVYDVHSPAVPDTEQILRQLRLGVAHLPLEQTWVNPDCGLKT
CHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCH
RRWEEVIPALKNMVAATQRLREELQAT
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIETQTLGYARMGKRRELKKALEAFWGGTLEAEALLATLQDLESQAWRTQRQANLDHIA
CCCCCCCCHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VGDQTLYDHVLDWATWLGLIPSRFQSLSGLERYFAMARGQEGLPALEMTKWFDTNYHYLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEC
PEIEADAIPQAHFDDFLATVRRAQGILGNRTSPVLLSPVTLLALSQRSGELRGDLEKLLP
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHCCCCHHHHHHHHHH
LYRELLQELQRLGIREVQVHEPILVTSEGKSLQEAVEQTYRWLASVGIPLHLVTYFDDLG
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
ETYPWAVELPVAGLSLDFTRGHNLELVRAHGFPADKILGAGVVDGRNIWQIQPKAVLATL
CCCCEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHCCCCCCCCCEEEECHHHHHHHH
QELQALAPNLRVQPSCSLQFVPHDAALETHLPEPLRNVLSFAEQKLAEVVLLARTLNGED
HHHHHHCCCCEECCCCCEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TAAQQQELEQQWQAFQDFNPPNSTVRQALANLTAQDFQRSLPYEQRIGRQVKLPPLPTTT
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEECCCCCCCC
IGSFPQTPEVRQWRVKYKKGEISQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
VEYFAQQLQGFAFTEHGWVQSYGSRCVRPPILYGDVSRPRPMTVREFKVAQSLTQKPVKG
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
MLTGPVTMLNWSFPRVDVSRREQALQIALALRAEVADLEAAGAAMVQVDEPALREGLPLK
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCC
KERWPEYLSWAVEAFRLTTAGAKPETQIHTHMCYSEFGDIIEHIERLDADVLSIENSRSS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
NKTLLQIAQAGYRHQVGNGVYDVHSPAVPDTEQILRQLRLGVAHLPLEQTWVNPDCGLKT
CHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCH
RRWEEVIPALKNMVAATQRLREELQAT
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA