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The map label for this gene is rumA

Identifier: 86604758

GI number: 86604758

Start: 23329

End: 24723

Strand: Reverse

Name: rumA

Synonym: CYA_0025

Alternate gene names: 86604758

Gene position: 24723-23329 (Counterclockwise)

Preceding gene: 86604759

Following gene: 86604757

Centisome position: 0.84

GC content: 62.51

Gene sequence:

>1395_bases
ATGGAGCAGCCAATCCCTTCTCTGGCCCCTGATCCCATCAAGCCGGGGGATCTCCTATCCCTGGAGATCACCGACCTAAC
CCCTGAGGGCGATGGCATTGCCCATACGCTTGGCCACGGGCGGGGTCGGGTGGTGTTTGTCGCCCAAGCGGTGCCAGGGG
ATCGCGTGGTGGCTCAGATAACTGAGATCAAGCGCAACTATCTGGTCGGGCGAATTCGCTCCCTCATAGCTCCCTCGCCG
CAGCGGGTGCGGCCGGCCTGCATTGTGGCGGACAAGTGCGGTGGCTGTCAGTGGCAGCACGTCAGCTACGCTCAGCAATT
GGTTACCAAGCAGCAGATCCTCAGGGATGCGCTGGAACGAATTGGCGGTCTTACTCTGCCGGAGCCTGTCCCGATTTTGG
GATCCCCGCAGCCGCTGCGCTACCGCAATAAGGTCACCTACCCCATTGCCAAAGCAGAGGGCCAGCCGCTGCGCATGGGC
TACTACCGACCGGGCAGCCACCGCCTGGTGAACCTCAACCAGTGCCCTGTGCAGGACGAGCGGCTGGATGTGTTCTTGCG
GGAAATTAAGCGGGATCTGCAGGAGGCCGGCTGGAGCGTCTACGACGAAAGCCGCCACCAGGGCCATCTGCGTCACTTGG
GCTTGCGCATTGGTCGCCGCACCCGCGAGGTGCTCCTCACCTTGGTCAGTTGCAGCCGCGACCTGCCCCACCTAGAAAGG
TGGGCCAAGCGCTGGATGCAGTGCTACCCCGACCTGGTGGGGGTTTGTCTCAACCTCAACCCGGCCCGCACCAACACCAT
CTTTGGCCCGCAAACGGAGGTGATTGCCGGGCGGGGATCCCTGCGGGAAGAATTGGCCGGTCTTTCCTTTTGGATTGACA
GCACCTCCTTCTTTCAAGTCAACACGGAGCAGGCGGAGGCCTTTTTTACCTGGATAGTGGATCAGCTCCAGCTGCGCGGC
GAGGAGATCGTCCTGGACGCCTACTGCGGCATCGGCACCCTAACCCTGCTCTTGGCCCGGCGGGCCAAACGGGCGATAGG
GGTGGAAGTCCTGCCGGCGGCGGTGGCCCAGGCCCGCTACAATGCCCAGTTCAACGGCATCCCCTCGGCCCAATTTTTCC
AGGGCGCAGTGGAAACGGTTTTGCCAACTCTGCCTCCTGCCGATATTGTGGTGTTGGATCCGCCGCGGCGCGGCTGCGAG
CGGCAGGTACTCGAGATCCTAAAGCAGCGACAACCCCAGCGCCTTGTCTACATTAGCTGTCACCCTGCCACCTTGGCGCG
CGACCTGCAAATTCTCATGGCCGAGGGATCCTACCGCCTAACCCAATGGCGAGCGGCCGACTTCTTTCCCCAAACAGGCC
ATGTAGAGGGGGTGGCCTTTTTGGAGCGGGTCTGA

Upstream 100 bases:

>100_bases
GATTTCGATTGACCAAAACTTTGGCGGCGCAGGCCCCGCCCTGAGGACTCTGTCCCGCTTGCGCGAATAGGGCTGGGGAG
AACGTCAACCACCTTTTTCC

Downstream 100 bases:

>100_bases
GGGCAACTCCAAAAACCTTGCCCAGCTTGCCGCAGAGCCGCCCTCTTGGTAAACGCAGCAGCCAGAGGCGACAAATGCTA
ACGTCAAAATAGCGACCTTA

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MEQPIPSLAPDPIKPGDLLSLEITDLTPEGDGIAHTLGHGRGRVVFVAQAVPGDRVVAQITEIKRNYLVGRIRSLIAPSP
QRVRPACIVADKCGGCQWQHVSYAQQLVTKQQILRDALERIGGLTLPEPVPILGSPQPLRYRNKVTYPIAKAEGQPLRMG
YYRPGSHRLVNLNQCPVQDERLDVFLREIKRDLQEAGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRDLPHLER
WAKRWMQCYPDLVGVCLNLNPARTNTIFGPQTEVIAGRGSLREELAGLSFWIDSTSFFQVNTEQAEAFFTWIVDQLQLRG
EEIVLDAYCGIGTLTLLLARRAKRAIGVEVLPAAVAQARYNAQFNGIPSAQFFQGAVETVLPTLPPADIVVLDPPRRGCE
RQVLEILKQRQPQRLVYISCHPATLARDLQILMAEGSYRLTQWRAADFFPQTGHVEGVAFLERV

Sequences:

>Translated_464_residues
MEQPIPSLAPDPIKPGDLLSLEITDLTPEGDGIAHTLGHGRGRVVFVAQAVPGDRVVAQITEIKRNYLVGRIRSLIAPSP
QRVRPACIVADKCGGCQWQHVSYAQQLVTKQQILRDALERIGGLTLPEPVPILGSPQPLRYRNKVTYPIAKAEGQPLRMG
YYRPGSHRLVNLNQCPVQDERLDVFLREIKRDLQEAGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRDLPHLER
WAKRWMQCYPDLVGVCLNLNPARTNTIFGPQTEVIAGRGSLREELAGLSFWIDSTSFFQVNTEQAEAFFTWIVDQLQLRG
EEIVLDAYCGIGTLTLLLARRAKRAIGVEVLPAAVAQARYNAQFNGIPSAQFFQGAVETVLPTLPPADIVVLDPPRRGCE
RQVLEILKQRQPQRLVYISCHPATLARDLQILMAEGSYRLTQWRAADFFPQTGHVEGVAFLERV
>Mature_464_residues
MEQPIPSLAPDPIKPGDLLSLEITDLTPEGDGIAHTLGHGRGRVVFVAQAVPGDRVVAQITEIKRNYLVGRIRSLIAPSP
QRVRPACIVADKCGGCQWQHVSYAQQLVTKQQILRDALERIGGLTLPEPVPILGSPQPLRYRNKVTYPIAKAEGQPLRMG
YYRPGSHRLVNLNQCPVQDERLDVFLREIKRDLQEAGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRDLPHLER
WAKRWMQCYPDLVGVCLNLNPARTNTIFGPQTEVIAGRGSLREELAGLSFWIDSTSFFQVNTEQAEAFFTWIVDQLQLRG
EEIVLDAYCGIGTLTLLLARRAKRAIGVEVLPAAVAQARYNAQFNGIPSAQFFQGAVETVLPTLPPADIVVLDPPRRGCE
RQVLEILKQRQPQRLVYISCHPATLARDLQILMAEGSYRLTQWRAADFFPQTGHVEGVAFLERV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=412, Percent_Identity=30.8252427184466, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI51173878, Length=412, Percent_Identity=30.8252427184466, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI269784680, Length=426, Percent_Identity=25.1173708920188, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI269784676, Length=426, Percent_Identity=25.1173708920188, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI20149688, Length=426, Percent_Identity=25.1173708920188, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI269784678, Length=368, Percent_Identity=25.5434782608696, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1789148, Length=461, Percent_Identity=28.4164859002169, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787083, Length=390, Percent_Identity=25.1282051282051, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790403, Length=179, Percent_Identity=29.0502793296089, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6322909, Length=442, Percent_Identity=22.8506787330317, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24666579, Length=421, Percent_Identity=27.5534441805226, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52037; Mature: 52037

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQPIPSLAPDPIKPGDLLSLEITDLTPEGDGIAHTLGHGRGRVVFVAQAVPGDRVVAQI
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHH
TEIKRNYLVGRIRSLIAPSPQRVRPACIVADKCGGCQWQHVSYAQQLVTKQQILRDALER
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGLTLPEPVPILGSPQPLRYRNKVTYPIAKAEGQPLRMGYYRPGSHRLVNLNQCPVQDE
HCCCCCCCCCCCCCCCCCCCCCCCEECEEECCCCCCEEEEEECCCCCCEEECCCCCCCHH
RLDVFLREIKRDLQEAGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRDLPHLER
HHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
WAKRWMQCYPDLVGVCLNLNPARTNTIFGPQTEVIAGRGSLREELAGLSFWIDSTSFFQV
HHHHHHHHCHHHHHHEECCCCCCCCCCCCCCCCEEECCCHHHHHHCCEEEEECCCEEEEE
NTEQAEAFFTWIVDQLQLRGEEIVLDAYCGIGTLTLLLARRAKRAIGVEVLPAAVAQARY
EHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
NAQFNGIPSAQFFQGAVETVLPTLPPADIVVLDPPRRGCERQVLEILKQRQPQRLVYISC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEE
HPATLARDLQILMAEGSYRLTQWRAADFFPQTGHVEGVAFLERV
CCHHHHHHHHHEEECCCEEEEEEEHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MEQPIPSLAPDPIKPGDLLSLEITDLTPEGDGIAHTLGHGRGRVVFVAQAVPGDRVVAQI
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHH
TEIKRNYLVGRIRSLIAPSPQRVRPACIVADKCGGCQWQHVSYAQQLVTKQQILRDALER
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGLTLPEPVPILGSPQPLRYRNKVTYPIAKAEGQPLRMGYYRPGSHRLVNLNQCPVQDE
HCCCCCCCCCCCCCCCCCCCCCCCEECEEECCCCCCEEEEEECCCCCCEEECCCCCCCHH
RLDVFLREIKRDLQEAGWSVYDESRHQGHLRHLGLRIGRRTREVLLTLVSCSRDLPHLER
HHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
WAKRWMQCYPDLVGVCLNLNPARTNTIFGPQTEVIAGRGSLREELAGLSFWIDSTSFFQV
HHHHHHHHCHHHHHHEECCCCCCCCCCCCCCCCEEECCCHHHHHHCCEEEEECCCEEEEE
NTEQAEAFFTWIVDQLQLRGEEIVLDAYCGIGTLTLLLARRAKRAIGVEVLPAAVAQARY
EHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
NAQFNGIPSAQFFQGAVETVLPTLPPADIVVLDPPRRGCERQVLEILKQRQPQRLVYISC
CCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEE
HPATLARDLQILMAEGSYRLTQWRAADFFPQTGHVEGVAFLERV
CCHHHHHHHHHEEECCCEEEEEEEHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]