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The map label for this gene is folK

Identifier: 86604753

GI number: 86604753

Start: 19706

End: 20212

Strand: Reverse

Name: folK

Synonym: CYA_0019

Alternate gene names: 86604753

Gene position: 20212-19706 (Counterclockwise)

Preceding gene: 86604754

Following gene: 86604751

Centisome position: 0.69

GC content: 65.88

Gene sequence:

>507_bases
ATGCAGCCCGACCTAGCCTACATTGCCCTGGGCAGCAACCTAGGGGATCCGCTGCAGCAATTGCGCTCGGCTGGGCAGAA
GTTGCTCAGGTTGGGCCAGGTGGTGGCTCGCTCCAGCCTCTACCGCAGCCCCGCCCAAGGGGGGCCACCCGGCCAGCCCG
ACTACCTCAATGCTGTGGTGGCGTTGGATCCCTTTCCCCCTTACCAATCGCCCCTGGAGTTGCTGAGGGCGCTGCTGGCC
ATCGAGCAGGAGGCAGGGCGAGTGCGGCGCATCCGCTGGGAGGCCAGGACCCTGGACTTGGATCTGCTGGCAATGGGCAA
CCGGATCCTGAAGAGCCCTGAGCTAACCCTGCCCCACCCTCGCATGATGGAGCGCCCTTTTGTCCTGGCCCCGCTGTGCG
AGATTGCTCCCCATTGGCGCCACCCCGAAACCGGCATAGCCGCAGCTGAGGCGCTGGCTGCCCTGGATAGCTCCTGGCTG
GAGCGCACTGACCTAGGCTGGGATTGA

Upstream 100 bases:

>100_bases
GACGGTGCGGGTGCACAAGCCGCAAGCCCCCCTGCCCGGTTTGTTCGACGATGTCTACGTGGAGATTGTCCGCCACCGTT
CTTCCAATAGCTAACCCTCG

Downstream 100 bases:

>100_bases
GCTCTTTAGCGCAGCCGCCATTTTCGCCGCCAGATCCACCAGCCGCGAGGCATAGCCGTACTCGTTGTCGTACCAGGAGA
GCACCTTCACGGTGCGATCC

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MQPDLAYIALGSNLGDPLQQLRSAGQKLLRLGQVVARSSLYRSPAQGGPPGQPDYLNAVVALDPFPPYQSPLELLRALLA
IEQEAGRVRRIRWEARTLDLDLLAMGNRILKSPELTLPHPRMMERPFVLAPLCEIAPHWRHPETGIAAAEALAALDSSWL
ERTDLGWD

Sequences:

>Translated_168_residues
MQPDLAYIALGSNLGDPLQQLRSAGQKLLRLGQVVARSSLYRSPAQGGPPGQPDYLNAVVALDPFPPYQSPLELLRALLA
IEQEAGRVRRIRWEARTLDLDLLAMGNRILKSPELTLPHPRMMERPFVLAPLCEIAPHWRHPETGIAAAEALAALDSSWL
ERTDLGWD
>Mature_168_residues
MQPDLAYIALGSNLGDPLQQLRSAGQKLLRLGQVVARSSLYRSPAQGGPPGQPDYLNAVVALDPFPPYQSPLELLRALLA
IEQEAGRVRRIRWEARTLDLDLLAMGNRILKSPELTLPHPRMMERPFVLAPLCEIAPHWRHPETGIAAAEALAALDSSWL
ERTDLGWD

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=142, Percent_Identity=45.7746478873239, Blast_Score=107, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324073, Length=159, Percent_Identity=36.4779874213836, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18615; Mature: 18615

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPDLAYIALGSNLGDPLQQLRSAGQKLLRLGQVVARSSLYRSPAQGGPPGQPDYLNAVV
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEE
ALDPFPPYQSPLELLRALLAIEQEAGRVRRIRWEARTLDLDLLAMGNRILKSPELTLPHP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCCCCCCCCCC
RMMERPFVLAPLCEIAPHWRHPETGIAAAEALAALDSSWLERTDLGWD
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQPDLAYIALGSNLGDPLQQLRSAGQKLLRLGQVVARSSLYRSPAQGGPPGQPDYLNAVV
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEE
ALDPFPPYQSPLELLRALLAIEQEAGRVRRIRWEARTLDLDLLAMGNRILKSPELTLPHP
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCCCCCCCCCC
RMMERPFVLAPLCEIAPHWRHPETGIAAAEALAALDSSWLERTDLGWD
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]