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The map label for this gene is norB

Identifier: 86361245

GI number: 86361245

Start: 580726

End: 582066

Strand: Direct

Name: norB

Synonym: RHE_PF00516

Alternate gene names: 86361245

Gene position: 580726-582066 (Clockwise)

Preceding gene: 86361244

Following gene: 86361246

Centisome position: 90.38

GC content: 58.91

Gene sequence:

>1341_bases
ATGAAATACAAGACACAAAGCGTCGCCATGCTCTATTTCTACGGAGCGCTCGGCCTCTTCATGGCGCAGCTGCTATTCGG
CGTCCTGGCGGGAACGATCTACGTTCTGCCGAATACGCTGCCGGTCGAGCTTCCGTTCAACATCGTCCGCATGATCCACA
CGAACGCGCTCATCGTCTGGCTGTTGATGGGCTTCATGGGCACCACCTATTTCCTGCTTCCGGAGGAGTGCGAGACGGAA
CTCTACAGCCCGAAACTCGCCATCGCGCAATTCTGGATCTTCCTGATAGCCGCAGCGGTCGCCGTCATAGGTTACCTGTT
CCACATCCACGAGGGCCGCGAGTTTCTCGAGCAGCCTTTCGCCATCAAGGTCGGCATCGTCGTCGTCGCCTTGATGTTCC
TGTTCAACGTGACGATGACGGCTTTGAAGGGGCGCAAGACCACCATCACCAACATCCTGCTGTTCGGGCTCTGGGGTGTG
GCGATCTTTTTCCTGTTTGCCTTCTACAACCCCGTCAACCTGTCGCTGGACAAGATGTACTGGTGGTATGTCGTCCATCT
GTGGGTCGAAGGCGTCTGGGAGCTGATCATGGCCTCGGTCCTGGCTTTCCTGATGATCAGGCTGAACGGCATCGACCGGG
AGGTCATCGAGAAATGGCTCTATGTGATCATCGGCCTGGCGCTCTTCTCCGGCATTCTCGGCACCGGCCATCACTATTAT
TGGATCGGTGCGCCCGGATACTGGCAGTGGATCGGCTCGCTTTTCTCGACGCTCGAGGTCGCCCCCTTCTTCACGATGGT
GATTTTCACCTTCGTCATGACCTGGAAGGCTGGCCGCAAGCATGAGAACAAGGCGGCGCTGTTGTGGTCGATCGGATGCT
CGGTCATGGCCTTCTTCGGCGCCGGCGTCTGGGGCTTCCTCCATACGCTGTCCTCGGTGAACTATTATACGCATGGCACG
CAGGTGACGGCGGCGCACGGGCACCTCGCCTTCTTCGGCGCCTACGTCATGCTCAATCTGGCGGCGATGGCCTATGCCAT
TCCTCAGATCCGCGGCCGTGCGCCCTATAATCAGTGGCTTTCGATCACGAGCTTCTGGATCATGTGCACGGCGATGTCGG
TGATGACCTTCGCCCTCACCTTTGCCGGCATCATCCAGGTTCATCTGCAGAGAATACTCGGCGAGGCCTATATGGACGTG
CAGGACCAGCTCGGCGTCTTCTACTGGGTGAGACTCGGCTCCGGCGTCTTCGTCTTCATCAGCGCGCTGATGTTCATCTG
GTCGGTCCTCGTTCCGGGCCGCGCCCGCCTGCCCGACGCAATCGCACAGCCCGCCGAATAG

Upstream 100 bases:

>100_bases
AAGTGAACGATCTCGCGGACTTCCTCGAATGGGTCAGCAAGATGAACACGCAGGCGTGGCCGCCGAACCAGGCGGGCTGA
CGGGCGAGAGGATCAAGCAA

Downstream 100 bases:

>100_bases
GCGGGCTCCCCCCTTTGGAGAACAGAGAAATGACGATCGCGCTTAATCTTCCGCTCAATCCTAATGCGGGAATTCCGCAC
TACACCGCCCATGGAAACGA

Product: nitric oxide reductase protein

Products: NA

Alternate protein names: NOR large subunit; Nitric oxide reductase cytochrome b subunit [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKYKTQSVAMLYFYGALGLFMAQLLFGVLAGTIYVLPNTLPVELPFNIVRMIHTNALIVWLLMGFMGTTYFLLPEECETE
LYSPKLAIAQFWIFLIAAAVAVIGYLFHIHEGREFLEQPFAIKVGIVVVALMFLFNVTMTALKGRKTTITNILLFGLWGV
AIFFLFAFYNPVNLSLDKMYWWYVVHLWVEGVWELIMASVLAFLMIRLNGIDREVIEKWLYVIIGLALFSGILGTGHHYY
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTFVMTWKAGRKHENKAALLWSIGCSVMAFFGAGVWGFLHTLSSVNYYTHGT
QVTAAHGHLAFFGAYVMLNLAAMAYAIPQIRGRAPYNQWLSITSFWIMCTAMSVMTFALTFAGIIQVHLQRILGEAYMDV
QDQLGVFYWVRLGSGVFVFISALMFIWSVLVPGRARLPDAIAQPAE

Sequences:

>Translated_446_residues
MKYKTQSVAMLYFYGALGLFMAQLLFGVLAGTIYVLPNTLPVELPFNIVRMIHTNALIVWLLMGFMGTTYFLLPEECETE
LYSPKLAIAQFWIFLIAAAVAVIGYLFHIHEGREFLEQPFAIKVGIVVVALMFLFNVTMTALKGRKTTITNILLFGLWGV
AIFFLFAFYNPVNLSLDKMYWWYVVHLWVEGVWELIMASVLAFLMIRLNGIDREVIEKWLYVIIGLALFSGILGTGHHYY
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTFVMTWKAGRKHENKAALLWSIGCSVMAFFGAGVWGFLHTLSSVNYYTHGT
QVTAAHGHLAFFGAYVMLNLAAMAYAIPQIRGRAPYNQWLSITSFWIMCTAMSVMTFALTFAGIIQVHLQRILGEAYMDV
QDQLGVFYWVRLGSGVFVFISALMFIWSVLVPGRARLPDAIAQPAE
>Mature_446_residues
MKYKTQSVAMLYFYGALGLFMAQLLFGVLAGTIYVLPNTLPVELPFNIVRMIHTNALIVWLLMGFMGTTYFLLPEECETE
LYSPKLAIAQFWIFLIAAAVAVIGYLFHIHEGREFLEQPFAIKVGIVVVALMFLFNVTMTALKGRKTTITNILLFGLWGV
AIFFLFAFYNPVNLSLDKMYWWYVVHLWVEGVWELIMASVLAFLMIRLNGIDREVIEKWLYVIIGLALFSGILGTGHHYY
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTFVMTWKAGRKHENKAALLWSIGCSVMAFFGAGVWGFLHTLSSVNYYTHGT
QVTAAHGHLAFFGAYVMLNLAAMAYAIPQIRGRAPYNQWLSITSFWIMCTAMSVMTFALTFAGIIQVHLQRILGEAYMDV
QDQLGVFYWVRLGSGVFVFISALMFIWSVLVPGRARLPDAIAQPAE

Specific function: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen red

COG id: COG3256

COG function: function code P; Nitric oxide reductase large subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heme-copper respiratory oxidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000883 [H]

Pfam domain/function: PF00115 COX1 [H]

EC number: =1.7.99.7 [H]

Molecular weight: Translated: 50513; Mature: 50513

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50855 COX1 ; PS00077 COX1_CUB ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKTQSVAMLYFYGALGLFMAQLLFGVLAGTIYVLPNTLPVELPFNIVRMIHTNALIVW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLMGFMGTTYFLLPEECETELYSPKLAIAQFWIFLIAAAVAVIGYLFHIHEGREFLEQPF
HHHHHHCCEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AIKVGIVVVALMFLFNVTMTALKGRKTTITNILLFGLWGVAIFFLFAFYNPVNLSLDKMY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
WWYVVHLWVEGVWELIMASVLAFLMIRLNGIDREVIEKWLYVIIGLALFSGILGTGHHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTFVMTWKAGRKHENKAALLWSIGCSVMAFFG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AGVWGFLHTLSSVNYYTHGTQVTAAHGHLAFFGAYVMLNLAAMAYAIPQIRGRAPYNQWL
HHHHHHHHHHHHCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SITSFWIMCTAMSVMTFALTFAGIIQVHLQRILGEAYMDVQDQLGVFYWVRLGSGVFVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHH
SALMFIWSVLVPGRARLPDAIAQPAE
HHHHHHHHHHCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MKYKTQSVAMLYFYGALGLFMAQLLFGVLAGTIYVLPNTLPVELPFNIVRMIHTNALIVW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
LLMGFMGTTYFLLPEECETELYSPKLAIAQFWIFLIAAAVAVIGYLFHIHEGREFLEQPF
HHHHHHCCEEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AIKVGIVVVALMFLFNVTMTALKGRKTTITNILLFGLWGVAIFFLFAFYNPVNLSLDKMY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
WWYVVHLWVEGVWELIMASVLAFLMIRLNGIDREVIEKWLYVIIGLALFSGILGTGHHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WIGAPGYWQWIGSLFSTLEVAPFFTMVIFTFVMTWKAGRKHENKAALLWSIGCSVMAFFG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AGVWGFLHTLSSVNYYTHGTQVTAAHGHLAFFGAYVMLNLAAMAYAIPQIRGRAPYNQWL
HHHHHHHHHHHHCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SITSFWIMCTAMSVMTFALTFAGIIQVHLQRILGEAYMDVQDQLGVFYWVRLGSGVFVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHH
SALMFIWSVLVPGRARLPDAIAQPAE
HHHHHHHHHHCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7508388; 2542222; 9521721 [H]