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The map label for this gene is fixKf

Identifier: 86361237

GI number: 86361237

Start: 572421

End: 573152

Strand: Direct

Name: fixKf

Synonym: RHE_PF00508

Alternate gene names: 86361237

Gene position: 572421-573152 (Clockwise)

Preceding gene: 86361221

Following gene: 86361238

Centisome position: 89.09

GC content: 59.7

Gene sequence:

>732_bases
ATGCTGATGCAGAACAACCACGCGTTCGACAATGCCGAGAAGACGTTAGAAGGCCTTCATGCCGGCCAATCGCTGTCGTC
GCTTTTCGTAAGTTCCACCGCCGAGGTCGTCGCAGCCGGCAAGGCCATTTGTTGGGAGGGCGACAAGGCCAACCATCTCT
TCCAGGTGGAAGAAGGCGTCGTCCGCCTGCATCGCATCATCGGCGAAGGTCGCCGGGTGATAACCGCATTTCATTTCGCC
GGCGATCTCATCGGCGCTTCCCTGCAAAACGATTTCCTGTTCACGGCCGAGGCCGTCACCGAATGCAAGATCCGCCGGAT
ATCTCGCAAGAGTTTCCACGAAGAAGTCGCCCGTTCGAATGCGCTCGGCCCGGCCTATATCTCGCTGCTCTGCCAGGAGG
CGGCCGCTGCGCATGAACAGATGGTGCTGCTGTCGAAGAAGAATGCCGAGGAGCGCCTCTGCAGCTTCATCGTCAAGCTT
GCATCCCGCCGCAACCCGCAGCCGCGTCAGGGCCTCTTGCGTGTCCCCATGAACCGCCAGGACATTGCCGACCATCTCGG
TCTGACCATCGAGACCGTCTCCCGCACGATCACGAAACTTGCCGCGCGCAACATCGTTGTTCCCGAGGGCAGGCACGACC
TCAGGATCGTCAATCTCGCCCGGCTGGTGCAACTGTCCGGCGACGCTGATGATTTTTCGGAGAATTCCTGTCATAGGGTC
AACCTGCATTGA

Upstream 100 bases:

>100_bases
TTACACACTGTTACAAACTTTCACCCTTTGGCACCGTCCGCTCATTCCGGTGTGACGCAGCGGCCTTCTACTGCACCCAC
TGCCACAAGGGGACACGACG

Downstream 100 bases:

>100_bases
TGCAACAAGCCAGGATCGACGTTCATGCCCCATCGCCTCGTGTCACCGAGAACCGCATCGCCGCAGGAGCTGGATGCCAT
GGTGCACGTGCTCGACGGCG

Product: nitrogen fixation transcriptional regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLMQNNHAFDNAEKTLEGLHAGQSLSSLFVSSTAEVVAAGKAICWEGDKANHLFQVEEGVVRLHRIIGEGRRVITAFHFA
GDLIGASLQNDFLFTAEAVTECKIRRISRKSFHEEVARSNALGPAYISLLCQEAAAAHEQMVLLSKKNAEERLCSFIVKL
ASRRNPQPRQGLLRVPMNRQDIADHLGLTIETVSRTITKLAARNIVVPEGRHDLRIVNLARLVQLSGDADDFSENSCHRV
NLH

Sequences:

>Translated_243_residues
MLMQNNHAFDNAEKTLEGLHAGQSLSSLFVSSTAEVVAAGKAICWEGDKANHLFQVEEGVVRLHRIIGEGRRVITAFHFA
GDLIGASLQNDFLFTAEAVTECKIRRISRKSFHEEVARSNALGPAYISLLCQEAAAAHEQMVLLSKKNAEERLCSFIVKL
ASRRNPQPRQGLLRVPMNRQDIADHLGLTIETVSRTITKLAARNIVVPEGRHDLRIVNLARLVQLSGDADDFSENSCHRV
NLH
>Mature_243_residues
MLMQNNHAFDNAEKTLEGLHAGQSLSSLFVSSTAEVVAAGKAICWEGDKANHLFQVEEGVVRLHRIIGEGRRVITAFHFA
GDLIGASLQNDFLFTAEAVTECKIRRISRKSFHEEVARSNALGPAYISLLCQEAAAAHEQMVLLSKKNAEERLCSFIVKL
ASRRNPQPRQGLLRVPMNRQDIADHLGLTIETVSRTITKLAARNIVVPEGRHDLRIVNLARLVQLSGDADDFSENSCHRV
NLH

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=199, Percent_Identity=31.1557788944724, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26923; Mature: 26923

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMQNNHAFDNAEKTLEGLHAGQSLSSLFVSSTAEVVAAGKAICWEGDKANHLFQVEEGV
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
VRLHRIIGEGRRVITAFHFAGDLIGASLQNDFLFTAEAVTECKIRRISRKSFHEEVARSN
HHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
ALGPAYISLLCQEAAAAHEQMVLLSKKNAEERLCSFIVKLASRRNPQPRQGLLRVPMNRQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHH
DIADHLGLTIETVSRTITKLAARNIVVPEGRHDLRIVNLARLVQLSGDADDFSENSCHRV
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
NLH
CCC
>Mature Secondary Structure
MLMQNNHAFDNAEKTLEGLHAGQSLSSLFVSSTAEVVAAGKAICWEGDKANHLFQVEEGV
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
VRLHRIIGEGRRVITAFHFAGDLIGASLQNDFLFTAEAVTECKIRRISRKSFHEEVARSN
HHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHC
ALGPAYISLLCQEAAAAHEQMVLLSKKNAEERLCSFIVKLASRRNPQPRQGLLRVPMNRQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHH
DIADHLGLTIETVSRTITKLAARNIVVPEGRHDLRIVNLARLVQLSGDADDFSENSCHRV
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
NLH
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]