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The map label for this gene is sigW [H]

Identifier: 86361152

GI number: 86361152

Start: 475398

End: 476108

Strand: Direct

Name: sigW [H]

Synonym: RHE_PF00422

Alternate gene names: 86361152

Gene position: 475398-476108 (Clockwise)

Preceding gene: 86361151

Following gene: 86361153

Centisome position: 73.99

GC content: 63.71

Gene sequence:

>711_bases
ATGACAGCAATTGCCGATCCGGTCGCCCGACGCCCCCAGAACATATCAGCAATTCCCGATGCCGAGCTCGTGTCCCTTGC
AAAGCAGGGTGACGAGCCCGCCATCCGCGCCATTGTGCAGCGTCACAACCGGCGCCTGTTCCGCACCGCGCGCGCCATCA
TCCGGAGCGATTGGGAAGCGGAAGACGTCGTGCAGGCCGTCTATGTCAAGGCCTTCACCAATCTGGCGACATTCCGCGGC
GAGGCCGAGCTCTCGACATGGCTGACCCGGATAACGCTTAACGAGGCGCTGGGCCGCGTTCGCGCACGAAAGAACACCAC
CGGGCTTGAGGAAATCGACATGCAGACGACATCGCGGGGTGGCGAAGTCCTCCAGTTCCCCTCCTCGCTCTCGGCAACCG
ATCCCGAAAGCGAGCTGTCGCGAAGCCAGGCGCGGCATCTTCTCGAACAGGCCGTCGACGACCTTCCGGACGAATTTCGC
GCCATCTTCGTGCTGCGCGAGGTCGAGGGCATGAGCACGGAGGAGGCCGCATCCTATCTCGGCATCAAACCTGAAACCGC
CAAGACCCGGCTGCATCGGGCGCGCAAGCTGATGCGCCAGTCGATCGAAAAACAGCTCTCCGGCGCTTTCTCTGCGCTGT
TCCCCTTCGACGGCGCCCGCTGTGCCTCCATGGGCGACCGCGTCATCGCAGCCCTTCGCCAGACATCGTGA

Upstream 100 bases:

>100_bases
AGCGGTCGATTTCTATTGCCGTTTTCATCCCGCCATGACGGGCAAGCTGGACGTCCGGCCGTAGGGCCGGGCTCCGATCC
AAAGCAATGCGGAGATTTGC

Downstream 100 bases:

>100_bases
GATCCGGTATCAGCACGCGATTGCTTGGACGCTGCAACGCGAGGCAGGCAGACCGTATGTTCGTCTCGAAATCATCAAGC
ATTCCAGACGGCGGCGCGGA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTAIADPVARRPQNISAIPDAELVSLAKQGDEPAIRAIVQRHNRRLFRTARAIIRSDWEAEDVVQAVYVKAFTNLATFRG
EAELSTWLTRITLNEALGRVRARKNTTGLEEIDMQTTSRGGEVLQFPSSLSATDPESELSRSQARHLLEQAVDDLPDEFR
AIFVLREVEGMSTEEAASYLGIKPETAKTRLHRARKLMRQSIEKQLSGAFSALFPFDGARCASMGDRVIAALRQTS

Sequences:

>Translated_236_residues
MTAIADPVARRPQNISAIPDAELVSLAKQGDEPAIRAIVQRHNRRLFRTARAIIRSDWEAEDVVQAVYVKAFTNLATFRG
EAELSTWLTRITLNEALGRVRARKNTTGLEEIDMQTTSRGGEVLQFPSSLSATDPESELSRSQARHLLEQAVDDLPDEFR
AIFVLREVEGMSTEEAASYLGIKPETAKTRLHRARKLMRQSIEKQLSGAFSALFPFDGARCASMGDRVIAALRQTS
>Mature_235_residues
TAIADPVARRPQNISAIPDAELVSLAKQGDEPAIRAIVQRHNRRLFRTARAIIRSDWEAEDVVQAVYVKAFTNLATFRGE
AELSTWLTRITLNEALGRVRARKNTTGLEEIDMQTTSRGGEVLQFPSSLSATDPESELSRSQARHLLEQAVDDLPDEFRA
IFVLREVEGMSTEEAASYLGIKPETAKTRLHRARKLMRQSIEKQLSGAFSALFPFDGARCASMGDRVIAALRQTS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=189, Percent_Identity=26.4550264550265, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 26253; Mature: 26122

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00024 HEMOPEXIN ; PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIADPVARRPQNISAIPDAELVSLAKQGDEPAIRAIVQRHNRRLFRTARAIIRSDWEA
CCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EDVVQAVYVKAFTNLATFRGEAELSTWLTRITLNEALGRVRARKNTTGLEEIDMQTTSRG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GEVLQFPSSLSATDPESELSRSQARHLLEQAVDDLPDEFRAIFVLREVEGMSTEEAASYL
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHC
GIKPETAKTRLHRARKLMRQSIEKQLSGAFSALFPFDGARCASMGDRVIAALRQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAIADPVARRPQNISAIPDAELVSLAKQGDEPAIRAIVQRHNRRLFRTARAIIRSDWEA
CCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EDVVQAVYVKAFTNLATFRGEAELSTWLTRITLNEALGRVRARKNTTGLEEIDMQTTSRG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GEVLQFPSSLSATDPESELSRSQARHLLEQAVDDLPDEFRAIFVLREVEGMSTEEAASYL
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHC
GIKPETAKTRLHRARKLMRQSIEKQLSGAFSALFPFDGARCASMGDRVIAALRQTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]