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The map label for this gene is ooxB

Identifier: 86361087

GI number: 86361087

Start: 400914

End: 402176

Strand: Direct

Name: ooxB

Synonym: RHE_PF00357

Alternate gene names: 86361087

Gene position: 400914-402176 (Clockwise)

Preceding gene: 86361086

Following gene: 86361088

Centisome position: 62.4

GC content: 63.42

Gene sequence:

>1263_bases
ATGATACACCCTGTGTCGACCCTACCCCCTTCCGCCTGTCGCGCCTCCTTGACGGGTCGAAGGTCAAGGTCGGCGCAATC
TGATCGTGAGGCCAGATCCGGAGAAGTCCGTGGGCGACCTGGGAAAGCTAGCGCAATGGATTACGACGTAATCGTCATCG
GCGGCGGGCTGGTAGGCGTATCGATAGCCTGGGGCTTGTCGAGATGCGGACAAAGGGTCGCAATCGTCGATGAAGGCGAC
GTGGCAAACAGGGCCGCCCGGGCGAATTTCGGTCTGGTCTGGAGCTCAAGCAAGGGCCTCGGCAACGCCGCCTATTCGCG
GTGGTCGCGAACCTCGACGGCGAACTGGCATCGGCTGGCAGCCGAGCTCAAGGCCGAAACGGGCATCGACACCGGTTTTG
TGCAGTCGGGCGGCTTCTCAATTCGGCTCACCTCAACGGAGCTGGAACGCGGGATCGCATCGATGGCGAAGCTCGCCGCC
CAACCAGGAATGGACGCGGAAGGAACGCCGCCGCTCGAATATGAGATACTCGACCAAGCGGAGACGCGCCGCCGTTTGCC
CCTGGTCGGCGACACAGTGGCAGGCGCTATTTTCAGCCCTCTCGACGGACACGCAAACCCGCTGCGGCTGTTCGCGGTCT
TTCATAAGGCGCTGCGGCAGCGCGGCGTCCATTATTTCCCGCATCGCAAGACGAATGCTATCCGCCATAGCGCCGGCGCT
TTCCAAGTGGCGGGAGAAGGATGGAGGCTCGGTGCCGGCAAGGTCGTGCTTGCAGCCGGCATCGGCAATACCGCGCTGGC
CGGGATCGTTGGGCTGAGCGTTCCGCTCTCGCCGAGCCGCGGCCAGATCATCGTCACCGAGCGGCTGAAGCCCTTCCTCA
GCCATGCCACTGGCTATCTGCGGCAGACAGACGAGGGCTCTGTGCTGATCGGCGAAAGCAAGGAGGCGGCGATCGATCAT
CAGCGCCCGCGAGGACCAATCACCGCCGTTCTGGCGGAACGCGCATTGCGCACCTTCCCACTGCTCAGGGACGCCAAGGC
GGTGCGGATCTGGGCCGCATTTCGTGTGGTGACGCCGGACGGCTACCCCATTTACGAGCAATCGGAGAGGTTTCCAGGTG
CTTTTGCATTCGCCTGCCATTCCGGCGTGACGCTGGCGGCGAACCATGCGCTGGTCCTGCCGCGGCAGATTTCGGCCGGG
GCGCTTTCTGACGATCTTTGCGTATTTCATTCCCGGAGGTTCCATGTTCCGGCGGCTGTCTGA

Upstream 100 bases:

>100_bases
GCTGACGGTGTGAGCGGGCTCTATCTGGCCGCGGGCTGCTCCGGCCATGGCTTCGGCCTTGGCCCGGCCATCGGCCTTCT
GGCGGCCGACCTCGTTGCCA

Downstream 100 bases:

>100_bases
TAGAAATGAGAGTACGGTCTCCTTCACATTCAACGCCGCGCCGACCACGGCCTGCAGCGGCGACAGCGTCGCTGCCGCAC
TGCTGGCTGCCGGACATTGC

Product: D-octopine oxidase, subunit B protein

Products: Dihydroxyacetone phosphate; Ubiquinol-8 [C]

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MIHPVSTLPPSACRASLTGRRSRSAQSDREARSGEVRGRPGKASAMDYDVIVIGGGLVGVSIAWGLSRCGQRVAIVDEGD
VANRAARANFGLVWSSSKGLGNAAYSRWSRTSTANWHRLAAELKAETGIDTGFVQSGGFSIRLTSTELERGIASMAKLAA
QPGMDAEGTPPLEYEILDQAETRRRLPLVGDTVAGAIFSPLDGHANPLRLFAVFHKALRQRGVHYFPHRKTNAIRHSAGA
FQVAGEGWRLGAGKVVLAAGIGNTALAGIVGLSVPLSPSRGQIIVTERLKPFLSHATGYLRQTDEGSVLIGESKEAAIDH
QRPRGPITAVLAERALRTFPLLRDAKAVRIWAAFRVVTPDGYPIYEQSERFPGAFAFACHSGVTLAANHALVLPRQISAG
ALSDDLCVFHSRRFHVPAAV

Sequences:

>Translated_420_residues
MIHPVSTLPPSACRASLTGRRSRSAQSDREARSGEVRGRPGKASAMDYDVIVIGGGLVGVSIAWGLSRCGQRVAIVDEGD
VANRAARANFGLVWSSSKGLGNAAYSRWSRTSTANWHRLAAELKAETGIDTGFVQSGGFSIRLTSTELERGIASMAKLAA
QPGMDAEGTPPLEYEILDQAETRRRLPLVGDTVAGAIFSPLDGHANPLRLFAVFHKALRQRGVHYFPHRKTNAIRHSAGA
FQVAGEGWRLGAGKVVLAAGIGNTALAGIVGLSVPLSPSRGQIIVTERLKPFLSHATGYLRQTDEGSVLIGESKEAAIDH
QRPRGPITAVLAERALRTFPLLRDAKAVRIWAAFRVVTPDGYPIYEQSERFPGAFAFACHSGVTLAANHALVLPRQISAG
ALSDDLCVFHSRRFHVPAAV
>Mature_420_residues
MIHPVSTLPPSACRASLTGRRSRSAQSDREARSGEVRGRPGKASAMDYDVIVIGGGLVGVSIAWGLSRCGQRVAIVDEGD
VANRAARANFGLVWSSSKGLGNAAYSRWSRTSTANWHRLAAELKAETGIDTGFVQSGGFSIRLTSTELERGIASMAKLAA
QPGMDAEGTPPLEYEILDQAETRRRLPLVGDTVAGAIFSPLDGHANPLRLFAVFHKALRQRGVHYFPHRKTNAIRHSAGA
FQVAGEGWRLGAGKVVLAAGIGNTALAGIVGLSVPLSPSRGQIIVTERLKPFLSHATGYLRQTDEGSVLIGESKEAAIDH
QRPRGPITAVLAERALRTFPLLRDAKAVRIWAAFRVVTPDGYPIYEQSERFPGAFAFACHSGVTLAANHALVLPRQISAG
ALSDDLCVFHSRRFHVPAAV

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI197927446, Length=436, Percent_Identity=24.5412844036697, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI21361378, Length=436, Percent_Identity=24.5412844036697, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI24797151, Length=283, Percent_Identity=27.208480565371, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130091, Length=247, Percent_Identity=26.7206477732794, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.1.99.5 [C]

Molecular weight: Translated: 44872; Mature: 44872

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHPVSTLPPSACRASLTGRRSRSAQSDREARSGEVRGRPGKASAMDYDVIVIGGGLVGV
CCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHH
SIAWGLSRCGQRVAIVDEGDVANRAARANFGLVWSSSKGLGNAAYSRWSRTSTANWHRLA
HHHHHHHHCCCEEEEEECCCHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHH
AELKAETGIDTGFVQSGGFSIRLTSTELERGIASMAKLAAQPGMDAEGTPPLEYEILDQA
HHHHHHCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCH
ETRRRLPLVGDTVAGAIFSPLDGHANPLRLFAVFHKALRQRGVHYFPHRKTNAIRHSAGA
HHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHCCCC
FQVAGEGWRLGAGKVVLAAGIGNTALAGIVGLSVPLSPSRGQIIVTERLKPFLSHATGYL
EEECCCCEEECCCCEEEEECCCCHHHHHHHEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
RQTDEGSVLIGESKEAAIDHQRPRGPITAVLAERALRTFPLLRDAKAVRIWAAFRVVTPD
EECCCCCEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
GYPIYEQSERFPGAFAFACHSGVTLAANHALVLPRQISAGALSDDLCVFHSRRFHVPAAV
CCCCCCCCCCCCCEEEEECCCCCEEECCCEEEECCCCCCCCCCCCHHEEECCCCCCCCCC
>Mature Secondary Structure
MIHPVSTLPPSACRASLTGRRSRSAQSDREARSGEVRGRPGKASAMDYDVIVIGGGLVGV
CCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHHH
SIAWGLSRCGQRVAIVDEGDVANRAARANFGLVWSSSKGLGNAAYSRWSRTSTANWHRLA
HHHHHHHHCCCEEEEEECCCHHHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHH
AELKAETGIDTGFVQSGGFSIRLTSTELERGIASMAKLAAQPGMDAEGTPPLEYEILDQA
HHHHHHCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCH
ETRRRLPLVGDTVAGAIFSPLDGHANPLRLFAVFHKALRQRGVHYFPHRKTNAIRHSAGA
HHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHHCCCC
FQVAGEGWRLGAGKVVLAAGIGNTALAGIVGLSVPLSPSRGQIIVTERLKPFLSHATGYL
EEECCCCEEECCCCEEEEECCCCHHHHHHHEEECCCCCCCCEEEEHHHHHHHHHHHHHHH
RQTDEGSVLIGESKEAAIDHQRPRGPITAVLAERALRTFPLLRDAKAVRIWAAFRVVTPD
EECCCCCEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCC
GYPIYEQSERFPGAFAFACHSGVTLAANHALVLPRQISAGALSDDLCVFHSRRFHVPAAV
CCCCCCCCCCCCCEEEEECCCCCEEECCCEEEECCCCCCCCCCCCHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glycerol 3-phosphate; Ubiquinone-8 [C]

Specific reaction: Glycerol 3-phosphate + Ubiquinone-8 --> Dihydroxyacetone phosphate + Ubiquinol-8 [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8045881 [H]