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The map label for this gene is groEL

Identifier: 86361060

GI number: 86361060

Start: 369683

End: 371311

Strand: Direct

Name: groEL

Synonym: RHE_PF00330

Alternate gene names: 86361060

Gene position: 369683-371311 (Clockwise)

Preceding gene: 86361059

Following gene: 86361063

Centisome position: 57.54

GC content: 60.71

Gene sequence:

>1629_bases
ATGGCTGCTAAAGAAGTCAAATTCAGCGTCGAAGCCCGCGAAAAAATGCTGCGCGGTGTCGACATCCTGGCAAACGCCGT
AAAGGTCACGCTCGGTCCGAAAGGCCGTAACGTGGTGATCGACAAGTCCTTCGGCGCGCCGCGCATCACCAAGGACGGCG
TTTCGGTCGCCAAGGAAATTGAACTTGAAGACAAGTTCGAAAACATGGGTGCGCAGATGGTCCGCGAAGTCGCTTCGAAG
ACCAGCGACATCGCGGGCGACGGCACCACGACCGCAACCGTTCTTGCCCAGGCGATCGTCAAGGAGGGCGCCAAGGCCGT
TACGTCAGGCATGAACCCGATGGACCTGAAGCGCGGAATCGATCTTGCCGTCAGTGCTATCGTCGAAGAACTGAAGGCCA
ACGCCCGCAAGATATCGAATAATGCCGAAATTGCCCAGGTCGGAACGATCTCGGCCAACGGCGATGCCGAAATCGGCCGC
TTCCTGGCGGAGGCCGTGCAAAAGGTCGGCAATGACGGTGTCATCACCGTTGAAGAAGCCAAGACCGCCGAAACCGAACT
CGAGGTCGTCGAAGGCATGCAGTTCGACCGCGGCTATTTGAGTCCCTATTTCGTCACCAATGCCGACAAGATGCGGGTCG
AATTCGAGGATCCCTATATCCTCATTCACGAGAAGAAGCTGTCGAACCTGCAGTCGATGCTGCCGATCCTCGAAGCCGTC
GTTCAGTCCGGCAAGCCGCTCCTCATCATCGCTGAAGATGTCGAAGGCGAAGCCCTTGCTACCCTCGTCGTCAACAAGCT
GCGCGGCGGCCTGAAGATCGCTGCCGTCAAGGCGCCCGGCTTCGGCGACCGCCGCAAGGCCATGCTCGAAGACATCGCCA
TCCTCACCGCCGGCACCGTCATCTCCGAAGATCTCGGCATCAAGCTTGAGAATGTGACGCTCAATATGCTCGGCCGGGCA
AAGAAGATCACGGTCGAGAAGGAAAACACCACCATCATCGACGGCGTCGGTTCGAAGGAGGAGATTTCGGGCCGCATCGC
CCAGATCAAGGCCCAGATCGAAGAGACCACCTCCGACTACGATCGCGAGAAGCTGCAGGAACGTCTTGCCAAACTCGCCG
GCGGTGTTGCCGTTATCCGCGTCGGCGGCTCGACGGAGGTCGAGGTGAAGGAGAAGAAGGATCGCGTCGACGACGCGCTT
CATGCAACAAGGGCAGCGGTCGAGGAAGGCATCCTGCCCGGTGGCGGCGTGGCGCTGCTGCGCGCGGTCAAGGCGCTCGA
CAATATCAAGACAGCCAATGACGACCAGCGCGTCGGCGTCGAAATCGTCCGCCGTGCGCTCGAGGCCCCGGCTCGCCAGA
TCGCCGAAAACGCCGGCGCGGAAGGCTCGGTCGTTGTCGGCAAGCTGCGCGAGAAGAGCGACTTCTCCTATGGCTGGAAT
GCCCAGACCGGCGAATTCGGCGATCTCTACGCGCAGGGCGTCATCGATCCAGCCAAGGTCGTTCGCACCGCGCTGCAGGA
CGCATCCTCCATCGCCGGCCTGCTGGTGACGACGGAAGCGATGATCGCCGAGAAGCCGAAGAAGGATGCTCCTCCGGCAA
TGCCGGCCGGCGCCGGTATGGACTTTTAA

Upstream 100 bases:

>100_bases
CGCTCCTCGCGCTTTGGATCTTAATTTCTACGCATGTCGTTCTCGCAAAACCGCTGTACACTTGGCGACATGCGCTAGCT
GCCGATGAAGGAGTGAGGAC

Downstream 100 bases:

>100_bases
GACAGGGTCGCAGGCTCGCCTGGAGACCCCGGGCGGGCCGTTTCCGAGGGAATGAAGATGGTTCAAACGATCCTAAGCCG
ATCGCAGGTGCCGCTGGACT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein 2; Protein Cpn60 2 [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MAAKEVKFSVEAREKMLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELEDKFENMGAQMVREVASK
TSDIAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGIDLAVSAIVEELKANARKISNNAEIAQVGTISANGDAEIGR
FLAEAVQKVGNDGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPILEAV
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTVISEDLGIKLENVTLNMLGRA
KKITVEKENTTIIDGVGSKEEISGRIAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDAL
HATRAAVEEGILPGGGVALLRAVKALDNIKTANDDQRVGVEIVRRALEAPARQIAENAGAEGSVVVGKLREKSDFSYGWN
AQTGEFGDLYAQGVIDPAKVVRTALQDASSIAGLLVTTEAMIAEKPKKDAPPAMPAGAGMDF

Sequences:

>Translated_542_residues
MAAKEVKFSVEAREKMLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELEDKFENMGAQMVREVASK
TSDIAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGIDLAVSAIVEELKANARKISNNAEIAQVGTISANGDAEIGR
FLAEAVQKVGNDGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPILEAV
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTVISEDLGIKLENVTLNMLGRA
KKITVEKENTTIIDGVGSKEEISGRIAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDAL
HATRAAVEEGILPGGGVALLRAVKALDNIKTANDDQRVGVEIVRRALEAPARQIAENAGAEGSVVVGKLREKSDFSYGWN
AQTGEFGDLYAQGVIDPAKVVRTALQDASSIAGLLVTTEAMIAEKPKKDAPPAMPAGAGMDF
>Mature_541_residues
AAKEVKFSVEAREKMLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEIELEDKFENMGAQMVREVASKT
SDIAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGIDLAVSAIVEELKANARKISNNAEIAQVGTISANGDAEIGRF
LAEAVQKVGNDGVITVEEAKTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPILEAVV
QSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTVISEDLGIKLENVTLNMLGRAK
KITVEKENTTIIDGVGSKEEISGRIAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDALH
ATRAAVEEGILPGGGVALLRAVKALDNIKTANDDQRVGVEIVRRALEAPARQIAENAGAEGSVVVGKLREKSDFSYGWNA
QTGEFGDLYAQGVIDPAKVVRTALQDASSIAGLLVTTEAMIAEKPKKDAPPAMPAGAGMDF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=53.6723163841808, Blast_Score=580, Evalue=1e-165,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=53.6723163841808, Blast_Score=580, Evalue=1e-165,
Organism=Homo sapiens, GI5453607, Length=548, Percent_Identity=23.1751824817518, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=66.7924528301887, Blast_Score=706, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=54.0642722117202, Blast_Score=580, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI193210679, Length=211, Percent_Identity=56.3981042654028, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17564182, Length=543, Percent_Identity=22.2836095764273, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17532603, Length=523, Percent_Identity=23.5181644359465, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=58.93536121673, Blast_Score=618, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6322350, Length=542, Percent_Identity=24.3542435424354, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322524, Length=195, Percent_Identity=24.1025641025641, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=56.8181818181818, Blast_Score=606, Evalue=1e-173,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=56.8181818181818, Blast_Score=606, Evalue=1e-173,
Organism=Drosophila melanogaster, GI45550936, Length=525, Percent_Identity=52.9523809523809, Blast_Score=569, Evalue=1e-162,
Organism=Drosophila melanogaster, GI45550132, Length=525, Percent_Identity=52.9523809523809, Blast_Score=569, Evalue=1e-162,
Organism=Drosophila melanogaster, GI45550935, Length=525, Percent_Identity=52.9523809523809, Blast_Score=569, Evalue=1e-162,
Organism=Drosophila melanogaster, GI17864606, Length=544, Percent_Identity=45.4044117647059, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24584129, Length=536, Percent_Identity=36.3805970149254, Blast_Score=351, Evalue=9e-97,
Organism=Drosophila melanogaster, GI19921262, Length=536, Percent_Identity=36.3805970149254, Blast_Score=351, Evalue=9e-97,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 57697; Mature: 57566

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKEVKFSVEAREKMLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEI
CCCCHHEEHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHC
ELEDKFENMGAQMVREVASKTSDIAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DLAVSAIVEELKANARKISNNAEIAQVGTISANGDAEIGRFLAEAVQKVGNDGVITVEEA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPILEAV
CCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCHH
ISEDLGIKLENVTLNMLGRAKKITVEKENTTIIDGVGSKEEISGRIAQIKAQIEETTSDY
HHHCCCCEEEEEEEEHHCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDALHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RAVKALDNIKTANDDQRVGVEIVRRALEAPARQIAENAGAEGSVVVGKLREKSDFSYGWN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
AQTGEFGDLYAQGVIDPAKVVRTALQDASSIAGLLVTTEAMIAEKPKKDAPPAMPAGAGM
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DF
CC
>Mature Secondary Structure 
AAKEVKFSVEAREKMLRGVDILANAVKVTLGPKGRNVVIDKSFGAPRITKDGVSVAKEI
CCCHHEEHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHC
ELEDKFENMGAQMVREVASKTSDIAGDGTTTATVLAQAIVKEGAKAVTSGMNPMDLKRGI
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DLAVSAIVEELKANARKISNNAEIAQVGTISANGDAEIGRFLAEAVQKVGNDGVITVEEA
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KTAETELEVVEGMQFDRGYLSPYFVTNADKMRVEFEDPYILIHEKKLSNLQSMLPILEAV
CCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHH
VQSGKPLLIIAEDVEGEALATLVVNKLRGGLKIAAVKAPGFGDRRKAMLEDIAILTAGTV
HHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCHH
ISEDLGIKLENVTLNMLGRAKKITVEKENTTIIDGVGSKEEISGRIAQIKAQIEETTSDY
HHHCCCCEEEEEEEEHHCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
DREKLQERLAKLAGGVAVIRVGGSTEVEVKEKKDRVDDALHATRAAVEEGILPGGGVALL
HHHHHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RAVKALDNIKTANDDQRVGVEIVRRALEAPARQIAENAGAEGSVVVGKLREKSDFSYGWN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
AQTGEFGDLYAQGVIDPAKVVRTALQDASSIAGLLVTTEAMIAEKPKKDAPPAMPAGAGM
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA