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The map label for this gene is purR [H]

Identifier: 86361018

GI number: 86361018

Start: 320585

End: 321622

Strand: Direct

Name: purR [H]

Synonym: RHE_PF00288

Alternate gene names: 86361018

Gene position: 320585-321622 (Clockwise)

Preceding gene: 86361017

Following gene: 86361020

Centisome position: 49.9

GC content: 63.29

Gene sequence:

>1038_bases
GTGGAACGTAAAGTCAATCTCAAAGATGTCGCACGCGATGCCGAAGTGTCGCTGAGCACGGCCTCGCACGCCCTGAACGG
CACTGCGCCGCTGACGATCGAAGTGCGGGAAAGAGTTCTGGATTCGGCAAAACGTCTCGGTTATCTCGACCGCCGGAGAA
AGAAGGCGACGATCGCCGCCTTGCACGTTCTGCTTCTCGCCATTCCTCACGATGCCGCGCCGGAGAGCGATCTCAATCTG
GTGAGCTGGACGATTCTCAATGGTCTTCGCAGGGAATGCGAGCGTCGCGGCATCCGCATCGTGCCCTACGTCAGCAGCGG
CCGGCGCATCGACGGCGCCGAGGTCAGGCAGATCGCCATATCAGAACGCGCTGATGGCATCGTGGTGCTGAATGATGATC
AGCCCGAACTCATCCGCAGCCTCGCCTCGGCGGATATGCCCGTCGTCATCGTCAACGGCGAGGATCCCGCCATGCTGGTC
GATACGGTGACACCTGAAAACCGCTTCGGCGCACGGCTCGGAATCGAGCACCTCCTGGCGCTTGGCCACCGCCGGATCCT
GCATCTGACCTGGAAGGGCCGCACGACCATCCAGCGCCGGTATGACGGTTTTTCCGATGCCTATTTCGCCGCACAGCTTC
CCGTGCCGAAGGATATGATCATCGAGGCGGAGGGATATGAGCCCCGGCATGGCGAAGCGGCCATCAATGCGCTGCTCCAT
CGCGACCCCACGCTGAAAGGCGCAACCGCCGTCTTCTGCGCGGCCGACAATCTGGCGCTCGGCTGCCTGAAGGCATTGGC
CGATCGCGATATACGCGTTCCCGACGCAATATCCGTGCTCGGCTTCGACGACATCGTTCCCGGCGAGTTCAGTCGCCCGC
CGCTGTCCACCGTGAGCGTGCCGACCGACCGTCTCGGCGCGGCAGCGCTCGCGCTCATCGAACAGCGGTTGATTGCCAAT
GATCCGCAGCGGCCGGCTCATCGTCTGGAGCTCGGCTGCCATCTCGTCCTGCGGGGCAGCATCGCACCGCCGCGCTAA

Upstream 100 bases:

>100_bases
AAAATATCAACGCAAGCACATGGCTTTTCTAAAGTCTTTATGTGAAAACATCGAGTTAGCTTATGCTTCGAAGGTACGAT
CTGAATTCCTGGCGGAAAAA

Downstream 100 bases:

>100_bases
AATGCCGGAAGAGCCAGCCTCTCGAGCTCCTAAGCTCACCTCAGCGCAAGCGCTCAGCCCGCAGCCGTGTATCGCGCGGG
CTTTCTCCAAAGGTGCTGCG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MERKVNLKDVARDAEVSLSTASHALNGTAPLTIEVRERVLDSAKRLGYLDRRRKKATIAALHVLLLAIPHDAAPESDLNL
VSWTILNGLRRECERRGIRIVPYVSSGRRIDGAEVRQIAISERADGIVVLNDDQPELIRSLASADMPVVIVNGEDPAMLV
DTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYFAAQLPVPKDMIIEAEGYEPRHGEAAINALLH
RDPTLKGATAVFCAADNLALGCLKALADRDIRVPDAISVLGFDDIVPGEFSRPPLSTVSVPTDRLGAAALALIEQRLIAN
DPQRPAHRLELGCHLVLRGSIAPPR

Sequences:

>Translated_345_residues
MERKVNLKDVARDAEVSLSTASHALNGTAPLTIEVRERVLDSAKRLGYLDRRRKKATIAALHVLLLAIPHDAAPESDLNL
VSWTILNGLRRECERRGIRIVPYVSSGRRIDGAEVRQIAISERADGIVVLNDDQPELIRSLASADMPVVIVNGEDPAMLV
DTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYFAAQLPVPKDMIIEAEGYEPRHGEAAINALLH
RDPTLKGATAVFCAADNLALGCLKALADRDIRVPDAISVLGFDDIVPGEFSRPPLSTVSVPTDRLGAAALALIEQRLIAN
DPQRPAHRLELGCHLVLRGSIAPPR
>Mature_345_residues
MERKVNLKDVARDAEVSLSTASHALNGTAPLTIEVRERVLDSAKRLGYLDRRRKKATIAALHVLLLAIPHDAAPESDLNL
VSWTILNGLRRECERRGIRIVPYVSSGRRIDGAEVRQIAISERADGIVVLNDDQPELIRSLASADMPVVIVNGEDPAMLV
DTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYFAAQLPVPKDMIIEAEGYEPRHGEAAINALLH
RDPTLKGATAVFCAADNLALGCLKALADRDIRVPDAISVLGFDDIVPGEFSRPPLSTVSVPTDRLGAAALALIEQRLIAN
DPQRPAHRLELGCHLVLRGSIAPPR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=350, Percent_Identity=28, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790194, Length=342, Percent_Identity=27.4853801169591, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=29.1291291291291, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790369, Length=362, Percent_Identity=25.6906077348066, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787906, Length=319, Percent_Identity=26.9592476489028, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789456, Length=318, Percent_Identity=24.5283018867925, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786540, Length=308, Percent_Identity=26.9480519480519, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789202, Length=301, Percent_Identity=26.2458471760797, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789068, Length=309, Percent_Identity=24.2718446601942, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787580, Length=339, Percent_Identity=24.7787610619469, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=25.5663430420712, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=26.5060240963855, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37780; Mature: 37780

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKVNLKDVARDAEVSLSTASHALNGTAPLTIEVRERVLDSAKRLGYLDRRRKKATIAA
CCCCCCHHHHHCCHHEEHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LHVLLLAIPHDAAPESDLNLVSWTILNGLRRECERRGIRIVPYVSSGRRIDGAEVRQIAI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHH
SERADGIVVLNDDQPELIRSLASADMPVVIVNGEDPAMLVDTVTPENRFGARLGIEHLLA
HCCCCEEEEECCCCHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
LGHRRILHLTWKGRTTIQRRYDGFSDAYFAAQLPVPKDMIIEAEGYEPRHGEAAINALLH
HCCCEEEEEEECCCCHHHHHCCCCCCCEEEEECCCCHHEEEEECCCCCCCHHHHHHHHHH
RDPTLKGATAVFCAADNLALGCLKALADRDIRVPDAISVLGFDDIVPGEFSRPPLSTVSV
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCEECC
PTDRLGAAALALIEQRLIANDPQRPAHRLELGCHLVLRGSIAPPR
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEEEECCCCCCC
>Mature Secondary Structure
MERKVNLKDVARDAEVSLSTASHALNGTAPLTIEVRERVLDSAKRLGYLDRRRKKATIAA
CCCCCCHHHHHCCHHEEHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LHVLLLAIPHDAAPESDLNLVSWTILNGLRRECERRGIRIVPYVSSGRRIDGAEVRQIAI
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHH
SERADGIVVLNDDQPELIRSLASADMPVVIVNGEDPAMLVDTVTPENRFGARLGIEHLLA
HCCCCEEEEECCCCHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
LGHRRILHLTWKGRTTIQRRYDGFSDAYFAAQLPVPKDMIIEAEGYEPRHGEAAINALLH
HCCCEEEEEEECCCCHHHHHCCCCCCCEEEEECCCCHHEEEEECCCCCCCHHHHHHHHHH
RDPTLKGATAVFCAADNLALGCLKALADRDIRVPDAISVLGFDDIVPGEFSRPPLSTVSV
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCEECC
PTDRLGAAALALIEQRLIANDPQRPAHRLELGCHLVLRGSIAPPR
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA