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The map label for this gene is ilvB

Identifier: 86361004

GI number: 86361004

Start: 297588

End: 299213

Strand: Direct

Name: ilvB

Synonym: RHE_PF00274

Alternate gene names: 86361004

Gene position: 297588-299213 (Clockwise)

Preceding gene: 86361003

Following gene: 86361008

Centisome position: 46.32

GC content: 61.56

Gene sequence:

>1626_bases
ATGAGCAGGAAAGAAACGACCGGCCAGGCGATCACCCGTTCGCTTGTCGCCCACGGAATTGACACGATCTTCGGCATCCC
CGGCGCCCATATGTATGATTTCAACGACGCGCTTTATGACGCCGGCGACAAGATCCGGTTCATTCACACCCGACATGAAC
AGGGCGCAGCCTACATGGCCTACGGCTATGCCAAATCCACCGGCCGGATCGGCGCCTATACAGTCGTCCCCGGCCCGGGC
GTCCTCAATTCCGGCGCGGCCCTCTGCACCGCCTACGGTGCCAATGCGCCGGTGCTCTGCATCACCGGCAACATCATGTC
GCACCTGATTGGCCGAGGCAGAGGACAGCTGCACGAACTTCCGGATCAGCTCGCGACGATGCGCGGGATTACCAAGACGG
CCGAGCGCATCAACCACCCGTCCGAGGCCGGTACAGTTATGGCTGGGCTCGTCGCCAAAATGCTCTCTGGCCGCCAAGGC
CCGGGAGCGGTCGAAGCCCCTTGGGACGTGTTCGGACAGTCCGCCCCTGAAATCGACGTTCGGGTTGGCGCGAGAGTGCC
TCATCCGGCCGTAAACCCCAATCAGATCGCCGCAGCAGCGGCGTTAATATCAGGCGCCAGCAATCCGATGATCATGGTCG
GTGGCGGCGCGGCGGATGCCAGCGCGGAGATCGCCGCGTTGGCGGAATTGCTGCAGGCGCCCGTCACCTCGCATCGCTCC
GGCAAGGGCATCGTCCCCGACGACCATCCGATTTATCTGAATTTCGTCGCCGCCTATGAATATTGGAAGAAGGTCGACGT
GCTGATCGGGATCGGCAGCCGGCTCGAATTGCAGTTCATGCGCTGGAAGTGGCTGCCCAAGGGGCTCAAGATCATCCGCA
TCGATGTCGACCCCACCGAAATGGTCCGCCTCAAGCCTGATCTCGGCATTGTCGCGGCCGCGAGAGATGGAACGCAGGCT
CTAGCCGATGCGGTGGCCGGCTCCCGCCGCGAGGATCGCACGCGCGAATTTGCCGAGCTCAATGATGAGGCAAAGTCTCG
TTTTTCGGCGGTGCAGCCGCAGCTTGGCTATCTCCAAGCCATTCGCGAGGCCCTGCCGAGAGACGGTTTCTTCGTCGAGG
AGATCAGCCAGATGGGCTTTACCGCCCGCTTCGCCTTCCCAGTCTACGGCCCTCGCCAATACGTGACATGCGGTTACCAG
GACAATCTTGGTTTCGGCTTCAACACCGCCTTAGGCGTGAAAGTCGCCCATCCCGACAAAGCCGTGATCTCCGTTTCGGG
CGATGGCGGCTTCATGTTCGGCGTCCAGGAACTTGCCACCGCCGTCCAGCACAAGATCGCCGTCGTCGCCATCGTGTTCA
ACAATTCCGCCTACGGCAATGTGCTGCGCGACCAGAAGCAAACCTATAAGGGCCGCACTCTCGGTTCGGATCTGACCAAT
CCCGATTTCGTCGCCCTCGGAGAGAGCTTCGGCATCCGATCCTTCAGGGCGACGAGCCCCGAAGAATTGAAAAACATACT
CGAGACAGCGCTCGCACTCGACGAGCCCGTCCTCATCGAAGTGCCCGTCGAAAAGGGATCGGAGGCGAGCCCCTGGCCCT
TCATCCATCCGGCTCCGCACGAATGA

Upstream 100 bases:

>100_bases
CTCGCCGGCATGCAGCCCGCCGATGCCGGCGCCATCGTCGCGGCGCTCCGGGCCGAAGGCTGCCTGAAATCATAACCGAA
TGCACCGTTGGAGCCTCCGA

Downstream 100 bases:

>100_bases
CCGGCAGGACGTGGAGCCCTATCAAGGCGATTTGAGCAAGACCGTCGCCAGCGGAGGCAGCGTCAGCCAGAGCGAATGCG
GCCTGCCGTGCGCGGGTACG

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSRKETTGQAITRSLVAHGIDTIFGIPGAHMYDFNDALYDAGDKIRFIHTRHEQGAAYMAYGYAKSTGRIGAYTVVPGPG
VLNSGAALCTAYGANAPVLCITGNIMSHLIGRGRGQLHELPDQLATMRGITKTAERINHPSEAGTVMAGLVAKMLSGRQG
PGAVEAPWDVFGQSAPEIDVRVGARVPHPAVNPNQIAAAAALISGASNPMIMVGGGAADASAEIAALAELLQAPVTSHRS
GKGIVPDDHPIYLNFVAAYEYWKKVDVLIGIGSRLELQFMRWKWLPKGLKIIRIDVDPTEMVRLKPDLGIVAAARDGTQA
LADAVAGSRREDRTREFAELNDEAKSRFSAVQPQLGYLQAIREALPRDGFFVEEISQMGFTARFAFPVYGPRQYVTCGYQ
DNLGFGFNTALGVKVAHPDKAVISVSGDGGFMFGVQELATAVQHKIAVVAIVFNNSAYGNVLRDQKQTYKGRTLGSDLTN
PDFVALGESFGIRSFRATSPEELKNILETALALDEPVLIEVPVEKGSEASPWPFIHPAPHE

Sequences:

>Translated_541_residues
MSRKETTGQAITRSLVAHGIDTIFGIPGAHMYDFNDALYDAGDKIRFIHTRHEQGAAYMAYGYAKSTGRIGAYTVVPGPG
VLNSGAALCTAYGANAPVLCITGNIMSHLIGRGRGQLHELPDQLATMRGITKTAERINHPSEAGTVMAGLVAKMLSGRQG
PGAVEAPWDVFGQSAPEIDVRVGARVPHPAVNPNQIAAAAALISGASNPMIMVGGGAADASAEIAALAELLQAPVTSHRS
GKGIVPDDHPIYLNFVAAYEYWKKVDVLIGIGSRLELQFMRWKWLPKGLKIIRIDVDPTEMVRLKPDLGIVAAARDGTQA
LADAVAGSRREDRTREFAELNDEAKSRFSAVQPQLGYLQAIREALPRDGFFVEEISQMGFTARFAFPVYGPRQYVTCGYQ
DNLGFGFNTALGVKVAHPDKAVISVSGDGGFMFGVQELATAVQHKIAVVAIVFNNSAYGNVLRDQKQTYKGRTLGSDLTN
PDFVALGESFGIRSFRATSPEELKNILETALALDEPVLIEVPVEKGSEASPWPFIHPAPHE
>Mature_540_residues
SRKETTGQAITRSLVAHGIDTIFGIPGAHMYDFNDALYDAGDKIRFIHTRHEQGAAYMAYGYAKSTGRIGAYTVVPGPGV
LNSGAALCTAYGANAPVLCITGNIMSHLIGRGRGQLHELPDQLATMRGITKTAERINHPSEAGTVMAGLVAKMLSGRQGP
GAVEAPWDVFGQSAPEIDVRVGARVPHPAVNPNQIAAAAALISGASNPMIMVGGGAADASAEIAALAELLQAPVTSHRSG
KGIVPDDHPIYLNFVAAYEYWKKVDVLIGIGSRLELQFMRWKWLPKGLKIIRIDVDPTEMVRLKPDLGIVAAARDGTQAL
ADAVAGSRREDRTREFAELNDEAKSRFSAVQPQLGYLQAIREALPRDGFFVEEISQMGFTARFAFPVYGPRQYVTCGYQD
NLGFGFNTALGVKVAHPDKAVISVSGDGGFMFGVQELATAVQHKIAVVAIVFNNSAYGNVLRDQKQTYKGRTLGSDLTNP
DFVALGESFGIRSFRATSPEELKNILETALALDEPVLIEVPVEKGSEASPWPFIHPAPHE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=558, Percent_Identity=26.1648745519713, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI21361361, Length=550, Percent_Identity=26, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=27.906976744186, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=29.4223826714801, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=30.2491103202847, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=28.1818181818182, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=25.7194244604317, Blast_Score=142, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=23.5500878734622, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=25.7142857142857, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=25.7142857142857, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=26.4184397163121, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6320816, Length=559, Percent_Identity=26.4758497316637, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=26.713532513181, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58061; Mature: 57930

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKETTGQAITRSLVAHGIDTIFGIPGAHMYDFNDALYDAGDKIRFIHTRHEQGAAYMA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCEEEEEEEECCCCCEEEE
YGYAKSTGRIGAYTVVPGPGVLNSGAALCTAYGANAPVLCITGNIMSHLIGRGRGQLHEL
EEEECCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCEEEEECHHHHHHHHCCCCHHHHH
PDQLATMRGITKTAERINHPSEAGTVMAGLVAKMLSGRQGPGAVEAPWDVFGQSAPEIDV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEE
RVGARVPHPAVNPNQIAAAAALISGASNPMIMVGGGAADASAEIAALAELLQAPVTSHRS
EECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHCCCC
GKGIVPDDHPIYLNFVAAYEYWKKVDVLIGIGSRLELQFMRWKWLPKGLKIIRIDVDPTE
CCCCCCCCCCEEEEHHHHHHHHHHEEEEEECCCCEEEEEEHEEECCCCCEEEEEECCHHH
MVRLKPDLGIVAAARDGTQALADAVAGSRREDRTREFAELNDEAKSRFSAVQPQLGYLQA
EEEECCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHH
IREALPRDGFFVEEISQMGFTARFAFPVYGPRQYVTCGYQDNLGFGFNTALGVKVAHPDK
HHHHCCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCC
AVISVSGDGGFMFGVQELATAVQHKIAVVAIVFNNSAYGNVLRDQKQTYKGRTLGSDLTN
EEEEEECCCCEEECHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
PDFVALGESFGIRSFRATSPEELKNILETALALDEPVLIEVPVEKGSEASPWPFIHPAPH
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
E
C
>Mature Secondary Structure 
SRKETTGQAITRSLVAHGIDTIFGIPGAHMYDFNDALYDAGDKIRFIHTRHEQGAAYMA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHCCCCEEEEEEEECCCCCEEEE
YGYAKSTGRIGAYTVVPGPGVLNSGAALCTAYGANAPVLCITGNIMSHLIGRGRGQLHEL
EEEECCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCEEEEECHHHHHHHHCCCCHHHHH
PDQLATMRGITKTAERINHPSEAGTVMAGLVAKMLSGRQGPGAVEAPWDVFGQSAPEIDV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEE
RVGARVPHPAVNPNQIAAAAALISGASNPMIMVGGGAADASAEIAALAELLQAPVTSHRS
EECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHCCCC
GKGIVPDDHPIYLNFVAAYEYWKKVDVLIGIGSRLELQFMRWKWLPKGLKIIRIDVDPTE
CCCCCCCCCCEEEEHHHHHHHHHHEEEEEECCCCEEEEEEHEEECCCCCEEEEEECCHHH
MVRLKPDLGIVAAARDGTQALADAVAGSRREDRTREFAELNDEAKSRFSAVQPQLGYLQA
EEEECCCCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHH
IREALPRDGFFVEEISQMGFTARFAFPVYGPRQYVTCGYQDNLGFGFNTALGVKVAHPDK
HHHHCCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCC
AVISVSGDGGFMFGVQELATAVQHKIAVVAIVFNNSAYGNVLRDQKQTYKGRTLGSDLTN
EEEEEECCCCEEECHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
PDFVALGESFGIRSFRATSPEELKNILETALALDEPVLIEVPVEKGSEASPWPFIHPAPH
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]