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The map label for this gene is aes [H]

Identifier: 86360794

GI number: 86360794

Start: 60421

End: 61404

Strand: Direct

Name: aes [H]

Synonym: RHE_PF00060

Alternate gene names: 86360794

Gene position: 60421-61404 (Clockwise)

Preceding gene: 86360793

Following gene: 86360795

Centisome position: 9.4

GC content: 64.13

Gene sequence:

>984_bases
GTGTCTCCGGGAGCAGCATCGCACCCGCGGCTGGCGTCGCTCACTTCCCACGAGGTCGGCGGCGCCCGCAGGCTCAATGC
CCTGCTGCGGCTCCTTCCCAGCTATAATACCGATCGACGCTGGAACGCCAAGACTGTGCAGGCCGCTATCCACATAGCGC
AACTCCTGGTGCCGGACGGGTTGGGGCGCCACAGGGACGTTGTCACTCGCGCTGTCTTGGCCGATGGCAAGCGCATCCGC
CTCAGGCTAATCCATCCGAACGGAGCCCGCCGCGGCCTCTACGTCCATTTTCACGGCGGCGCCTGGGTCATGGGCAACGC
CCGCCTTGACGACGGCATCACCCGCCCGATCGCCAAAGACTGTCAGGTGGTGGTGGCCGGCGTCGATTTTCATAATGCCG
CCGACGACCGGCTAGACCTTGCTCTTCTGGACAGCAAGGCGTCGATCGAATGGCTGGCCGATCATCTCGCCGAGTTCGAG
GTCGAGCATCTCGTCCTCGGCGGCGAGTCATCAGGCGCTCAACTCGCTGCAGAAGCGCTTCTTCATCTGCGCCAATGCCG
CAAGGCGGAGGCCGTCGTCGGATTTGTTTCGGTGTGCGGCGCTTTCGATCTCGGCGGTTCACAAAGCCTGCGGCTCTCGA
CGGGCAGAAGTCTGGTGATAGACGGGCAATCGGCGCTCCGCAATCTGCAGCGACTGACGCCAAGCCTTTCCGGGCGCGAG
GCGAAAGGTCCGTTGTCTGCCGATCTCTCGGGCCTGCCTCCGGCCCTGTTGGTCGCGGGGGCGCTCGATCCGATCCTCGA
CGACAGCATCTTGATGTATGAGCGATGGCAGAGCCAAAACGGCAATGCCGACTGCGTCGTCTTCCCGGAGGCCCCGCACG
GCTTCAACCGCTTCCCGACCAAACTGGCAGCCAGAGCAAAAAGCCTTGTCAGAGCGTGGATATCGCAGACGCTCGACCGC
ACTGCGAGTGCGGGTAGGGGCTGA

Upstream 100 bases:

>100_bases
ATGGGCCTGATGGGGCGCTGAAGCGCGCGTCGCAGAAGATCCTGCCGGACCTTAGAATGCATCTAACGCGAATCCGAGGG
CTGGCAGCGAAGTGAGGGAC

Downstream 100 bases:

>100_bases
TCGAGCGCAGGCCTACGAGTCTGTACGCTATTGCACAATGTATTGCTCAATTCTAGACGGTTAGGTTCGCCCGCAGCAAG
GGTCGCATCACTGATGCGCG

Product: hypothetical protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSPGAASHPRLASLTSHEVGGARRLNALLRLLPSYNTDRRWNAKTVQAAIHIAQLLVPDGLGRHRDVVTRAVLADGKRIR
LRLIHPNGARRGLYVHFHGGAWVMGNARLDDGITRPIAKDCQVVVAGVDFHNAADDRLDLALLDSKASIEWLADHLAEFE
VEHLVLGGESSGAQLAAEALLHLRQCRKAEAVVGFVSVCGAFDLGGSQSLRLSTGRSLVIDGQSALRNLQRLTPSLSGRE
AKGPLSADLSGLPPALLVAGALDPILDDSILMYERWQSQNGNADCVVFPEAPHGFNRFPTKLAARAKSLVRAWISQTLDR
TASAGRG

Sequences:

>Translated_327_residues
MSPGAASHPRLASLTSHEVGGARRLNALLRLLPSYNTDRRWNAKTVQAAIHIAQLLVPDGLGRHRDVVTRAVLADGKRIR
LRLIHPNGARRGLYVHFHGGAWVMGNARLDDGITRPIAKDCQVVVAGVDFHNAADDRLDLALLDSKASIEWLADHLAEFE
VEHLVLGGESSGAQLAAEALLHLRQCRKAEAVVGFVSVCGAFDLGGSQSLRLSTGRSLVIDGQSALRNLQRLTPSLSGRE
AKGPLSADLSGLPPALLVAGALDPILDDSILMYERWQSQNGNADCVVFPEAPHGFNRFPTKLAARAKSLVRAWISQTLDR
TASAGRG
>Mature_326_residues
SPGAASHPRLASLTSHEVGGARRLNALLRLLPSYNTDRRWNAKTVQAAIHIAQLLVPDGLGRHRDVVTRAVLADGKRIRL
RLIHPNGARRGLYVHFHGGAWVMGNARLDDGITRPIAKDCQVVVAGVDFHNAADDRLDLALLDSKASIEWLADHLAEFEV
EHLVLGGESSGAQLAAEALLHLRQCRKAEAVVGFVSVCGAFDLGGSQSLRLSTGRSLVIDGQSALRNLQRLTPSLSGREA
KGPLSADLSGLPPALLVAGALDPILDDSILMYERWQSQNGNADCVVFPEAPHGFNRFPTKLAARAKSLVRAWISQTLDRT
ASAGRG

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=245, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 35126; Mature: 34994

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPGAASHPRLASLTSHEVGGARRLNALLRLLPSYNTDRRWNAKTVQAAIHIAQLLVPDG
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LGRHRDVVTRAVLADGKRIRLRLIHPNGARRGLYVHFHGGAWVMGNARLDDGITRPIAKD
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHC
CQVVVAGVDFHNAADDRLDLALLDSKASIEWLADHLAEFEVEHLVLGGESSGAQLAAEAL
CEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHH
LHLRQCRKAEAVVGFVSVCGAFDLGGSQSLRLSTGRSLVIDGQSALRNLQRLTPSLSGRE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECHHHHHHHHHHCCCCCCCC
AKGPLSADLSGLPPALLVAGALDPILDDSILMYERWQSQNGNADCVVFPEAPHGFNRFPT
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCHH
KLAARAKSLVRAWISQTLDRTASAGRG
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPGAASHPRLASLTSHEVGGARRLNALLRLLPSYNTDRRWNAKTVQAAIHIAQLLVPDG
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
LGRHRDVVTRAVLADGKRIRLRLIHPNGARRGLYVHFHGGAWVMGNARLDDGITRPIAKD
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEECCCEEEEECCCCCCCCCCCHHHC
CQVVVAGVDFHNAADDRLDLALLDSKASIEWLADHLAEFEVEHLVLGGESSGAQLAAEAL
CEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHH
LHLRQCRKAEAVVGFVSVCGAFDLGGSQSLRLSTGRSLVIDGQSALRNLQRLTPSLSGRE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEECHHHHHHHHHHCCCCCCCC
AKGPLSADLSGLPPALLVAGALDPILDDSILMYERWQSQNGNADCVVFPEAPHGFNRFPT
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCCHH
KLAARAKSLVRAWISQTLDRTASAGRG
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA